Making microenvironments: A look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications

Low, but not too low, oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human dermal fibroblast culture

(2016) Abhigyan Satyam et al.   Acta Biomaterialia

DNA conformational behavior and compaction in biomimetic systems: Toward better understanding of DNA packaging in cell

(2016) Anatoly Zinchenko   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Two-Dimensional Crowding Uncovers a Hidden Conformation of α-Synuclein

(2016) Priya R. Banerjee et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Calcineurin in a Crowded World

(2016) Erik C. Cook et al.   BIOCHEMISTRY

Macromolecular crowding favors the fibrillization of β2-microglobulin by accelerating the nucleation step and inhibiting fibril disassembly

(2016) Xu-Dong Luo et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Macromolecular Crowding Modulates Actomyosin Kinetics

(2016) Jinghua Ge et al.   BIOPHYSICAL JOURNAL

Crowding Activates Heat Shock Protein 90

(2016) Jackson C. Halpin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The interplay of intrinsic disorder and macromolecular crowding on α-synuclein fibril formation

(2016) Nobu C. Shirai et al.   JOURNAL OF CHEMICAL PHYSICS

Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract

(2016) Guang-Ying Sun et al.   JOURNAL OF SEPARATION SCIENCE

ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs

(2016) Michelle H. Lee et al.   Scientific Reports

Macromolecular Crowding Regulates the Gene Expression Profile by Limiting Diffusion

(2016) Mahdi Golkaram et al.   PLoS Computational Biology

Macromolecular crowding meets oxygen tension in human mesenchymal stem cell culture - A step closer to physiologically relevant in vitro organogenesis

(2016) Daniela Cigognini et al.   Scientific Reports

Macromolecular crowding-assisted fabrication of liquid-crystalline imprinted polymers

(2015) Chen Zhang et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Challenge of Mimicking the Influences of the Cellular Environment on RNA Structure by PEG-Induced Macromolecular Crowding

(2015) Jillian Tyrrell et al.   BIOCHEMISTRY

Macromolecular crowding: Macromolecules friend or foe

(2015) Shruti Mittal et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding

(2015) Marina C. Prewitz et al.   BIOMATERIALS

Mimicking the in vivo Environment – The Effect of Crowding on RNA and Biomacromolecular Folding and Activity

(2015) Erica Fiorini et al.   CHIMIA

PEG-induced molecular crowding leads to a relaxed conformation, higher thermal stability and lower catalytic efficiency of Escherichia coli β-galactosidase

(2015) Verónica Nolan et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Macromolecular crowding conditions enhance glycation and oxidation of whey proteins in ultrasound-induced Maillard reaction

(2015) Marija Perusko et al.   FOOD CHEMISTRY

Effect of Dextran 70 on the thermodynamic and structural properties of proteins

(2015) Gurumayum Suraj Sharma et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Synergistic Rate Boosting of Collagen Fibrillogenesis in Heterogeneous Mixtures of Crowding Agents

(2015) Jean-Yves Dewavrin et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Molecular crowding effects on conformation and stability of G-quadruplex DNA structure: Insights from molecular dynamics simulation

(2015) A. Verdian Doghaei et al.   JOURNAL OF THEORETICAL BIOLOGY

A sensor for quantification of macromolecular crowding in living cells

(2015) Arnold J Boersma et al.   NATURE METHODS

Macromolecular crowding creates heterogeneous environments of gene expression in picolitre droplets

(2015) Maike M. K. Hansen et al.   Nature Nanotechnology

Cosolvent and crowding effects on the polymerization kinetics of actin

(2015) Christopher Rosin et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Interactions between Skeletal Muscle Myoblasts and their Extracellular Matrix Revealed by a Serum Free Culture System

(2015) Vishal Chaturvedi et al.   PLoS One

Analysis of the size dependence of macromolecular crowding shows that smaller is better

(2015) Kim A. Sharp   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kinetics of polymer looping with macromolecular crowding: effects of volume fraction and crowder size

(2015) Jaeoh Shin et al.   Soft Matter

Making More Matrix: Enhancing the Deposition of Dermal–Epidermal Junction ComponentsIn Vitroand Accelerating Organotypic Skin Culture Development, Using Macromolecular Crowding

(2015) Paula Benny et al.   TISSUE ENGINEERING PART A

Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies

(2015) Pramod Kumar et al.   Scientific Reports

Cell-derived matrices for tissue engineering and regenerative medicine applications

(2015) Lindsay E. Fitzpatrick et al.   Biomaterials Science

Tuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowding

(2014) Jean-Yves Dewavrin et al.   Acta Biomaterialia

Macromolecular Crowding Meets Tissue Engineering by Self-Assembly: A Paradigm Shift in Regenerative Medicine

(2014) Abhigyan Satyam et al.   ADVANCED MATERIALS

Intracellular crowding effects on the self-association of the bacterial cell division protein FtsZ

(2014) Lamis Naddaf et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Macromolecular Crowding as a Regulator of Gene Transcription

(2014) Hiroaki Matsuda et al.   BIOPHYSICAL JOURNAL

Applying Molecular Crowding Models to Simulations of Virus Capsid Assembly In Vitro

(2014) Gregory R. Smith et al.   BIOPHYSICAL JOURNAL

Differential subcellular and extracellular localisations of proteins required for insulin-like growth factor- and extracellular matrix-induced signalling events in breast cancer progression

(2014) Helen C Plant et al.   BMC CANCER

The cytoplasmic cage domain of the mechanosensitive channel MscS is a sensor of macromolecular crowding

(2014) Ian Rowe et al.   JOURNAL OF GENERAL PHYSIOLOGY

Macromolecular Crowding Effect upon in Vitro Enzyme Kinetics: Mixed Activation–Diffusion Control of the Oxidation of NADH by Pyruvate Catalyzed by Lactate Dehydrogenase

(2014) Cristina Balcells et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria

(2014) Matthias Graupp et al.   LARYNGOSCOPE

Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation

(2014) Rafi Rashid et al.   TISSUE ENGINEERING PART C-METHODS

Enhancement of biological reactions on cell surfaces via macromolecular crowding

(2014) Rafi Chapanian et al.   Nature Communications

The crowd you're in with: Effects of different types of crowding agents on protein aggregation

(2013) Leonid Breydo et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Off-the-shelf human decellularized tissue-engineered heart valves in a non-human primate model

(2013) Benedikt Weber et al.   BIOMATERIALS

Influence of crowded cellular environments on protein folding, binding, and oligomerization: Biological consequences and potentials of atomistic modeling

(2013) Huan-Xiang Zhou   FEBS LETTERS

Increased Ribozyme Activity in Crowded Solutions

(2013) Ravi Desai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantitative influence of macromolecular crowding on gene regulation kinetics

(2013) M. Tabaka et al.   NUCLEIC ACIDS RESEARCH

Macromolecular Crowding Amplifies Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells by Enhancing the Pro-Adipogenic Microenvironment

(2013) Xiu Min Ang et al.   TISSUE ENGINEERING PART A

Macromolecular Crowding Effect on Cartilaginous Matrix Production: A Comparison of Two-Dimensional and Three-Dimensional Models

(2013) Bo Chen et al.   TISSUE ENGINEERING PART C-METHODS

Impact of macromolecular crowding on DNA replication

(2013) Barak Akabayov et al.   Nature Communications

The biology of the extracellular matrix

(2012) Dirk Hubmacher et al.   CURRENT OPINION IN RHEUMATOLOGY

Human fibroblast matrices bio-assembled under macromolecular crowding support stable propagation of human embryonic stem cells

(2012) Yanxian Peng et al.   Journal of Tissue Engineering and Regenerative Medicine

Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior

(2012) Adam S. Zeiger et al.   PLoS One

Antioxidation of Decellularized Stem Cell Matrix Promotes Human Synovium-Derived Stem Cell-Based Chondrogenesis

(2012) Ming Pei et al.   STEM CELLS AND DEVELOPMENT

Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies

(2011) Clarice Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

The extracellular matrix at a glance

(2010) C. Frantz et al.   JOURNAL OF CELL SCIENCE

Effect of Macromolecular Crowding on Reaction Rates: A Computational and Theoretical Study

(2009) Jun Soo Kim et al.   BIOPHYSICAL JOURNAL

The Scar-in-a-Jar: studying potential antifibrotic compounds from the epigenetic to extracellular level in a single well

(2009) CZC Chen et al.   BRITISH JOURNAL OF PHARMACOLOGY

Folding, Stability and Shape of Proteins in Crowded Environments: Experimental and Computational Approaches

(2009) Antonios Samiotakis et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids–in silico predictions and in vitro evidence

(2009) Karthik S. Harve et al.   NUCLEIC ACIDS RESEARCH

Efficacy of solvent extraction methods for acellularization of embryoid bodies

(2008) Rekha Nair et al.   JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

Analysis of the osteoinductive capacity and angiogenicity of anin vitrogenerated extracellular matrix

(2008) Quynh P. Pham et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A