Article
Multidisciplinary Sciences
Zhiyou Zong, Xinzhuo Liu, Zhuo Ye, Dingbin Liu
Summary: This study presents a double-switch pH-low insertion peptide (D-S pHLIP) system to selectively harvest cTME-EVs from the blood serum of tumor mouse models, overcoming the current technical challenges in cTME-EVs research. The cTME-EVs anchored by D-S pHLIP were magnetically enriched and analyzed with high-resolution mRNA sequencing, resulting in a significantly higher number of differentially expressed TME-related genes and hub genes compared to the gold-standard ultracentrifugation. This work could have a significant impact on TME research and clinical liquid biopsy for cancer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Chemistry, Multidisciplinary
Sara Hassanpour Tamrin, Amir Sanati Nezhad, Arindom Sen
Summary: Exosomes are cell-derived structures containing lipids, proteins, and nucleic acids, playing roles in physiological and pathological processes. Microfluidic technology offers simple, cost-effective, precise, and fast processing for exosome isolation, yet faces challenges hindering their clinical utility.
Article
Chemistry, Analytical
Haimeng Pei, Zhaojun Han, Yuan Wang, Chang Xu, Yong Li, Yuanyuan Fan, Lu Li, Bo Tang
Summary: This study presents a label-free microfluidic method to isolate low-adhesion cells with stem properties and evaluate CSC-specific drugs. The method is highly versatile and allows for the isolation of cancer stem-like cells and the evaluation of CSC-specific drugs on a large scale.
ANALYTICAL CHEMISTRY
(2023)
Article
Biology
Samantha C. Schwager, Katherine M. Young, Lauren A. Hapach, Caroline M. Carlson, Jenna A. Mosier, Tanner J. McArdle, Wenjun Wang, Curtis Schunk, Anissa L. Jayathilake, Madison E. Bates, Francois Bordeleau, Marc A. Antonyak, Richard A. Cerione, Cynthia A. Reinhart-King
Summary: Weakly migratory cancer cells disseminate from the primary tumor via communication with stromal cells and enhance cancer cell migration and metastasis by releasing microvesicles rich in Tg2.
Article
Chemistry, Multidisciplinary
Jingjing Ding, Guihong Lu, Weidong Nie, Li-Li Huang, Yahui Zhang, Wenlin Fan, Guanghao Wu, Houli Liu, Hai-Yan Xie
Summary: An engineered self-activatable photo-EV for synergistic trimodal anticancer therapy has been reported, which utilizes M1 EVs to simultaneously load multiple drugs, achieving synergism of immunotherapy, photodynamic therapy, and chemotherapy with great promise in fighting cancers.
ADVANCED MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Yawei Zhang, Liang Zhao, Yaocheng Li, Shuangshuang Wan, Zhiyao Yuan, Guangyue Zu, Fei Peng, Xianguang Ding
Summary: Extracellular vesicles (EVs) serve as attractive bioinformatic nanocabinets for liquid biopsy, but current challenges include inefficient methods for EV enrichment and low sensitivity of EV bioinformatic decoding technologies. New EV nanotechnology is actively being developed to overcome these challenges and promote clinical application.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xingle Yu, Xiaohui Chen, Zixin Sun, Ruyan Niu, Yun Deng, Liu Wang, Ying Zhu, Liangliang Zhang, Hong Zhang, Kang Wang, Jichun Yang, Wei Gu, Guoxiang Liu, Yang Luo
Summary: This study proposed a DNAzyme-triggered assembly and disassembly system for the conversion of single nano-sized sEVs into clusters, which could be enriched by centrifugation and then broken into single sEVs in the presence of magnesium ions. The quantification of sEVs was achieved by recording the increase in fluorescence upon nucleic acid cleavage, with a detection limit as low as 54 particles/μL. The entire process could be completed in 1.5 hours without the need for ultracentrifugation.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Lingyun Feng, Lijuan Guo, Yoshimasa Tanaka, Li Su
Summary: sEVs are important mediators in cell-to-cell communication and play a crucial role in cancer development and progression. Tumor-derived sEVs regulate processes such as proliferation, metastasis, drug resistance, and immunosuppression, and have a significant impact on the tumor microenvironment. They have the potential to be used as biomarkers and therapeutic targets for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
I. -Ying Kuo, Chih-Hsiung Hsieh, Wan-Ting Kuo, Chih-Peng Chang, Yi-Ching Wang
Summary: Cells in the tumor microenvironment require a class of checkpoints to regulate various secretion and transport processes. Recent studies have shown that Rab proteins mediate communication between diverse cargoes and substrates through vesicular trafficking and secretion pathways in the tumor microenvironment. This review focuses on the role and regulation of vesicular secretion pathways in the tumor microenvironment, and describes the latest research advances and future directions.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Oncology
Chuanshi He, Ling Li, Linlin Wang, Wanrong Meng, Yaying Hao, Guiquan Zhu
Summary: The article reviews the role of exosome-mediated interactions between cells under radiotherapy conditions, highlighting the importance of exosomes in the tumor microenvironment and the rebuilding of regulatory processes under radiation stimulation.
CANCER BIOLOGY & MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Masatoshi Ito, Kai Kudo, Hiroshi Higuchi, Hiroko Otsuka, Masayuki Tanaka, Nahoko Fukunishi, Takuma Araki, Masako Takamatsu, Yoko Ino, Yayoi Kimura, Ai Kotani
Summary: The study revealed that EVs derived from EBV-transformed lymphomas contain immunomodulatory proteins and phospholipids associated with TAM formation, which may contribute to the development of lymphomas.
Review
Oncology
Abhimanyu Thakur, Amanda Johnson, Emily Jacobs, Kui Zhang, Jonathan Chen, Zhubo Wei, Qizhou Lian, Huanhuan Joyce Chen
Summary: Exosomal communication in the tumor microenvironment is crucial for cancer development, progression, and metastasis. Exosomes can communicate with neighboring cells or distant organs, promoting tumor growth and cancer progression. Recent studies have identified potential energy sources for exosomes, including mitochondria, adenosine triphosphate, and glycolytic enzymes. This review emphasizes the importance of exosome energy sources and how they maintain structural integrity and stability during communication.
Article
Oncology
Alexander Cocks, Filippo Del Vecchio, Verena Martinez-Rodriguez, Monique Schukking, Muller Fabbri
Summary: This review article discusses the interaction between tumor-associated macrophages (TAMs) and cancer cells, with a focus on the pro-tumoral phenotype elicited by EV-contained miRNAs released by TAMs and transferred to cancer cells.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Hee Cheol Yang, Yoo Min Ham, Jeong Ah Kim, Won Jong Rhee
Summary: A novel single-step, equipment-free EV concentration method using super absorbent polymer (SAP) beads was developed in this study, which efficiently concentrates EVs while maintaining high purity of the EV solution. The method was shown to successfully enrich EVs in human urine samples and culture medium, outperforming conventional ultrafiltration methods.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Oncology
Ling Zhang, Mengchi Sun, Zhonggui He, Jin Sun, Heran Li, Qiuhua Luo
Summary: Extracellular vesicles (EVs) can serve as carriers to enhance the efficacy and specificity of cancer immunotherapy (CIT). By modulating tumor immunity, EVs have the potential to improve low response rates and individual differences in CIT. The development of biohybrid EVs shows promise in overcoming deficits in tumor immunity.
Article
Biochemical Research Methods
Laura Marcu, Stephen A. Boppart, Mark R. Hutchinson, Juergen Popp, Brian C. Wilson
JOURNAL OF BIOMEDICAL OPTICS
(2018)
Article
Biochemical Research Methods
Stephen A. Boppart, J. Quincy Brown, Camile S. Farah, Esther Kho, Laura Marcu, Christobel M. Saunders, Henricus J. C. M. Sterenborg
JOURNAL OF BIOMEDICAL OPTICS
(2018)
Article
Optics
Fredrick A. South, Yuan-Zhi Liu, Andrew J. Bower, Yang Xu, P. Scott Carney, Stephen A. Boppart
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2018)
Article
Medical Laboratory Technology
Sarah J. Erickson-Bhatt, Kelly J. Mesa, Marina Marjanovic, Eric J. Chaney, Adeel Ahmad, Pin-Chieh Huang, Z. George Liu, Kelly Cunningham, Stephen A. Boppart
TRANSLATIONAL RESEARCH
(2018)
Article
Multidisciplinary Sciences
Sixian You, Haohua Tu, Eric J. Chaney, Yi Sun, Youbo Zhao, Andrew J. Bower, Yuan-Zhi Liu, Marina Marjanovic, Saurabh Sinha, Yang Pu, Stephen A. Boppart
NATURE COMMUNICATIONS
(2018)
Article
Biochemical Research Methods
Jungeun Won, Guillermo L. Monroy, Pin-Chieh Huang, Roshan Dsouza, Malcolm C. Hill, Michael A. Novak, Ryan G. Porter, Eric Chaney, Ronit Barkalifa, Stephen A. Boppart
BIOMEDICAL OPTICS EXPRESS
(2018)
Article
Biochemical Research Methods
Sixian You, Yi Sun, Eric J. Chaney, Youbo Zhao, Jianxin Chen, Stephen A. Boppart, Haohua Tu
BIOMEDICAL OPTICS EXPRESS
(2018)
Article
Biochemical Research Methods
Sixian You, Yi Sun, Eric J. Chaney, Youbo Zhao, Jianxin Chen, Stephen A. Boppart, Haohua Tu
BIOMEDICAL OPTICS EXPRESS
(2018)
Article
Multidisciplinary Sciences
Yi Sun, Sixian You, Haohua Tu, Darold R. Spillman, Eric J. Chaney, Marina Marjanovic, Joanne Li, Ronit Barkalifa, Jianfeng Wang, Anna M. Higham, Natasha N. Luckey, Kimberly A. Cradock, Z. George Liu, Stephen A. Boppart
Article
Radiology, Nuclear Medicine & Medical Imaging
Yuan Liu, Haohua Tu, Sixian You, Eric J. Chaney, Marina Marjanovic, Stephen A. Boppart
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2019)
Article
Optics
Yi Sun, Haohua Tu, Sixian You, Chi Zhang, Yuan-Zhi Liu, Stephen A. Boppart
Article
Optics
Stephen A. Boppart, Sixian You, Lianhuang Li, Jianxin Chen, Haohua Tu
Article
Multidisciplinary Sciences
Sixian You, Ronit Barkalifa, Eric J. Chaney, Haohua Tu, Jaena Park, Janet Elise Sorrells, Yi Sun, Yuan-Zhi Liu, Lin Yang, Danny Z. Chen, Marina Marjanovic, Saurabh Sinha, Stephen A. Boppart
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Chemistry, Analytical
Jungeun Won, Guillermo L. Monroy, Roshan I. Dsouza, Darold R. Spillman, Jonathan McJunkin, Ryan G. Porter, Jindou Shi, Edita Aksamitiene, MaryEllen Sherwood, Lindsay Stiger, Stephen A. Boppart
Summary: Middle ear infection is a common inflammatory disease, with subjective diagnostic methods currently in use. To improve diagnosis, researchers have integrated optical coherence tomography technology into a handheld imaging probe to non-invasively and quantitatively assess middle ear infections.
Article
Radiology, Nuclear Medicine & Medical Imaging
Yi Sun, Sixian You, Xiaoxi Du, Allison Spaulding, Z. George Liu, Eric J. Chaney, Darold R. Spillman Jr, Marina Marjanovic, Haohua Tu, Stephen A. Boppare
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2020)