Article
Microbiology
Shikha Pachauri, Rinat Zaid, Pramod D. Sherkhane, Jamela Easa, Ada Viterbo, Ilan Chet, Benjamin A. Horwitz, Prasun K. Mukherjee
Summary: Trichoderma virens is a beneficial fungus that enhances crop yields by helping plants fight pathogens and abiotic stresses. The P and Q strains of T. virens have distinct genetic and phenotypic differences, with the Q strain producing strong antimicrobial agents.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Yu Liang, Dongyan Li, Yuyi Zheng, Yong Shen, Qin Li, Mengsha Wei, Haojie Yang, Saiyi Ye, Chunmei Chen, Hucheng Zhu, Yonghui Zhang
Summary: A document investigation on the fungus Trichoderma virens led to the isolation of thirteen undescribed carotane sesquiterpenes and homologues. Their structures were elucidated and absolute configurations were assigned. Two new branched compounds and novel compounds derived from ring rearrangements/expansions and ring cleavage were discovered. The compounds were also evaluated for their biological activities.
Article
Chemistry, Organic
Min Liang, Hai-Ning Lyu, Zi-Ying Ma, Er-Wei Li, Lei Cai, Wen-Bing Yin
Summary: Two potential non-ribosomal peptide synthetases (NRPSs) were identified from the genome of a guanophilic fungus Amphichorda guana through bioinformatics analysis and gene knockout experiments. Subsequent LC-MS guided isolation led to the discovery of a novel cyclodepsipeptide, isaridin H (1), with significant antifungal activity against Botrytis cinerea and Alternaria solani.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Review
Microbiology
Sheridan L. Woo, Rosa Hermosa, Matteo Lorito, Enrique Monte
Summary: This review discusses the ecophysiology and diversity of Trichoderma and the complexity of its relationships in the agroecosystem. Trichoderma has the potential as a direct and indirect biological control agent, biostimulant, and biofertilizer, which are useful for agricultural applications. Considerations are also presented on advancing Trichoderma applications in real-world scenarios, contributing towards eco-sustainable agriculture.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Wenyu Han, Zhongshou Wu, Zhenhui Zhong, Jason Williams, Steven E. Jacobsen, Zuodong Sun, Yi Tang
Summary: A comprehensive analysis of natural product biosynthetic gene clusters (BGCs) from the biocontrol fungus ThT22 revealed its versatile biosynthetic capacity. The biosynthesis pathway of a previously unknown compound eujavanicol A was identified through heterologous expression. The activation of silent BGCs in ThT22 was also demonstrated through transcriptomics and metabolic analysis, facilitating further exploration of the functional roles of natural products from ThT22.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Alessia Staropoli, Giuseppina Iacomino, Paola De Cicco, Sheridan L. Woo, Luigi Di Costanzo, Francesco Vinale
Summary: A bioactive chromone derivative with antibiotic and antitumor activities was isolated from Trichoderma harzianum M10 using OSMAC strategy. It showed significant growth inhibition against Rhizoctonia solani and reduced the viability of colorectal cancer cells in a concentration-dependent manner. Metabolomic analysis also revealed the modulation of several compounds by the cultivating conditions.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Chemistry, Organic
Dexiu Yan, Yudai Matsuda
Summary: Heterologous expression in the fungus Aspergillus funiculosus CBS 116.56 led to the discovery of four new meroterpenoids, funiculolides A-D, with funiculolide D featuring an unusual spirocyclopentanone moiety synthesized by the iron and alpha-ketoglutarate-dependent dioxygenase FncG.
Article
Plant Sciences
Guo-Bang Li, Jing Fan, Jin-Long Wu, Jia-Xue He, Jie Liu, Shuai Shen, Zeeshan Ghulam Nabi Gishkori, Xiao-Hong Hu, Yong Zhu, Shi-Xin Zhou, Yun-Peng Ji, Mei Pu, Jing-Hao Zhao, Zhi-Xue Zhao, He Wang, Ji-Wei Zhang, Yan-Yan Huang, Yan Li, Fu Huang, Wen-Ming Wang
Summary: Ustilaginoidea virens is a biotrophic fungal pathogen that colonizes rice floral organs and causes false smut disease. The pathogen uses a secreted protein UvCBP1 to manipulate plant immunity, compromising chitin-induced defense responses in Arabidopsis and rice.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Organic
Haochen Zou, Xin Xia, Qiushuang Xu, Haoxin Wang, Yuemao Shen, Yaoyao Li
Summary: The structural diversity of PoTeMs is mainly generated by cyclases and cytochrome P450s. In Saccharopolyspora hirsuta, six new cytotoxic PoTeMs, including the first examples of oxidized one-ring PoTeMs, were discovered through combinatorial reassembling and heterologous expression.
Article
Biochemistry & Molecular Biology
Gagan D. Gupta, Ravindra Bansal, Hiral Mistry, Bharati Pandey, Prasun K. Mukherjee
Summary: Trichoderma virens colonizes roots and forms a symbiotic relationship with plants, deriving nutrients from plants and offering defense in return. Tsp1, a cysteine-rich protein, acts as an inducer of susceptibility in plants. Evolutionary analysis suggests Tsp1 belongs to a novel effector protein family.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Rabea Schweiger, Fabiola Padilla-Arizmendi, Guillermo Nogueira-Lopez, Michael Rostas, Robert Lawry, Chris Brown, John Hampton, Johanna M. Steyaert, Caroline Mueller, Artemio Mendoza-Mendoza
Summary: The study investigated the interactions between maize and the endophytic fungus Trichoderma virens, revealing that fungal genotype can influence the metabolic composition of roots and is associated with two fungal genes. Colonization by T. virens leads to profound metabolic changes in plant roots, possibly contributing to systemic endophyte effects observed in later plant developmental stages.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Chemistry, Organic
Fangfang Jia, Mingliang Zhang, Jiangping Fan, Yang Wang, Xiangyu Ge, Xinyu Mi, Xiao Liu, Juan Wang, Peng-Fei Tu, She-Po Shi
Summary: Two novel 3,5-dimethylorsellinic acid derived meroterpenoids (peniciterpenolides A and B), one new spongian-type diterpene (peniciterpenolide C), and one new drimane-type sesquiterpene (peniciterpenolide D), along with a known meroterpenoid (atlantinone B), were isolated from an endophytic fungus identified from Huperzia serrata. These compounds exhibited inhibitory activity against nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophage cells with IC50 values ranging from 20.6 to 46.9 μM.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Plant Sciences
Monica De Palma, Teresa Docimo, Gianpiero Guida, Maria Salzano, Rossella Albrizio, Pasquale Giorio, Michelina Ruocco, Marina Tucci
Summary: Trichoderma spp. can enhance plant performance under water stress by modulating transcription factors and genes related to photosynthesis, antioxidant defences, and maintenance of a juvenile state. The fungi specifically affected genes involved in stress damage mitigation, helping plants recover growth and photosynthesis levels after stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Chiu-Yueh Hung, Chuanshu Zhu, Farooqahmed S. Kittur, Maotao He, Erland Arning, Jianhui Zhang, Asia J. Johnson, Gurpreet S. Jawa, Michelle D. Thomas, Tomas T. Ding, Jiahua Xie
Summary: The pathophysiology of Huntington's disease (HD), caused by the mutant huntingtin protein (mHtt), is still not fully understood. This study explored the early pathogenic events induced by expanded polyglutamine tracts in plants and mice models. The findings suggest that impaired GTP cyclohydrolase I (GTPCH) expression and folate metabolism may play a role in the pathogenesis of HD.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Organic
Lan Jiang, Xue Zhang, Yuya Sato, Guoliang Zhu, Atsushi Minami, Weiyan Zhang, Taro Ozaki, Bin Zhu, Zhixin Wang, Xinye Wang, Kangjie Lv, Jingyu Zhang, Yongheng Wang, Shushan Gao, Chengwei Liu, Tom Hsiang, Lixin Zhang, Hideaki Oikawa, Xueting Liu
Summary: Two previously unreported fungal bifunctional terpene synthases, FoFS and AtAS, have been discovered in this study. FoFS catalyzes the formation of fusoxypenes A-C and (-)-astellatene, while AtAS synthesizes preaspterpenacid I. The enantioselective synthesis of sesterterpenes and the cyclization pathways of the fusoxypenes were also characterized.
