Journal
CHEMISTRYSELECT
Volume 2, Issue 6, Pages 2044-2054Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/slct.201601270
Keywords
Azo-bond cleavage; Cu ''-azo dye; HCT116 cells ~; MCF-7 cells; MOLT-4 cells; topo I inhibition
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Funding
- University Grants Commission, New Delhi [39-749/2010]
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Toxicity of azo dyes and theirinteraction with biological systems has either been overlooked or not exploited properly. A Cu '' complex of 2-(2-hydroxyphenylazo)-indole-3/-acetic acid ( HPIA) was synthesized to see role of metal ions on azo toxicity. It was characterized with UV-Vis, IR, EPR, mass spectrometry, elemental and thermogravimetric analysis. Evidence suggest formation of a bis-azo complex with stability constant logb=12.88. DFT calculations based on spectroscopic evidence suggest 1: 2:: CuII:HPIA complex. Enzyme assay on reductive cleavage of the azo bond shows complex forms less amines than HPIA. Binding of complex to DNA was similar to HPIA. As the complex restricts reduction of azo bond to toxic amines and has comparable binding with DNA it could be less cytotoxic. Cis and trans HPIA and the complex were treated to normal HEK293T cells; activity was comparable at low concentrations. When compounds were treated to human colon carcinomaHCT116 cells, ALL MOLT-4 human leukemia cells and MCF-7 breast cancer cells activity of the complex was better than cisor trans HPIA. The study revealed complex formation of HPIA with CuII targets carcinoma cellsmore than HPIA.
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