Article
Biochemistry & Molecular Biology
Marianna Tyczewska, Patrycja Sujka-Kordowska, Marta Szyszka, Karol Jopek, Malgorzata Blatkiewicz, Ludwik K. Malendowicz, Marcin Rucinski
Summary: The homeostasis of the adrenal gland is crucial for its proper functioning in both non-stressful and stressful conditions, involving interactions between different types of cells. However, there is insufficient information regarding marker gene expression in the rat adrenal gland under non-stressful conditions. This study aimed to determine the expression of marker genes in different zones of the rat adrenal gland and revealed distinct expression patterns of interstitial cell markers in specific zones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shawn Miller Jr, Juan E. Abrahante, Avtar Roopra, Brendan J. Dougherty
Summary: Gonadectomy rapidly and persistently reduces circulating sex steroids, allowing for the interrogation of hormonal influence on neural function in ventral spinal cord tissue of adult rats using a next-generation RNA sequencing dataset.
Article
Biology
Yunhe Liu, Aoshen Wu, Xueqing Peng, Xiaona Liu, Gang Liu, Lei Liu
Summary: The developed scRNA simulation algorithm is able to recreate the actual data generation process, discover the impact of parameters on classification, compare normalization and clustering algorithms, and provide new insights into scRNA analysis.
Article
Anatomy & Morphology
Natalia Lagunas, Jose Manuel Fernandez-Garcia, Noemi Blanco, Antonio Ballesta, Beatriz Carrillo, Maria-Angeles Arevalo, Paloma Collado, Helena Pinos, Daniela Grassi
Summary: Sex steroid hormones exert organizational and activational effects on the brain and peripheral tissues. Estradiol, testosterone, and their metabolites exert their biological action through genomic and non-genomic mechanisms. The expression of estrogen receptors (ERs) and androgen receptors (AR) differs between males and females, suggesting a sex-dependent regulation. This study investigates the effects of estrogens and androgens on the pituitary and adrenal glands, and suggests that neonatal modification of these pathways can lead to permanent changes in neuroendocrine functions.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Biochemistry & Molecular Biology
Vadim Tseilikman, Maria Komelkova, Marina V. Kondashevskaya, Eugenia Manukhina, H. Fred Downey, Valerii Chereshnev, Margarita Chereshneva, Pavel Platkovskii, Anna Goryacheva, Anton Pashkov, Julia Fedotova, Olga Tseilikman, Natalya Maltseva, Olga Cherkasova, Charlotte Steenblock, Stefan R. Bornstein, Barbara Ettrich, George P. Chrousos, Enrico Ullmann
Summary: This study found high- and low-anxiety phenotypes in rats exposed to predator scent stress, with high-anxiety phenotype showing changes in adrenal function and histomorphology, while low-anxiety phenotype did not.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Hui-Yang Liao, Xuan Xiao, Rui Peng, Juan Le, Hai-Bo Wang, Shao-Ting Wang
Summary: Two new derivatization reactions were designed in this study to improve the feasible LC-MS/MS method for simultaneous quantification of 15 multiclass steroids. The method showed proper sample consumption, satisfactory sensitivity, and high throughput.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
N. Shukla, M. F. Levine, G. Gundem, D. Domenico, B. Spitzer, N. Bouvier, J. E. Arango-Ossa, D. Glodzik, J. S. Medina-Martinez, U. Bhanot, J. Gutierrez-Abril, Y. Zhou, E. Fiala, E. Stockfisch, S. Li, M. Rodriguez-Sanchez, T. O'Donohue, C. Cobbs, M. H. A. Roehrl, J. Benhamida, F. Iglesias Cardenas, M. Ortiz, M. Kinnaman, S. Roberts, M. Ladanyi, S. Modak, S. Farouk-Sait, E. Slotkin, M. A. Karajannis, F. Dela Cruz, J. Glade Bender, A. Zehir, A. Viale, M. F. Walsh, A. L. Kung, E. Papaemmanuil
Summary: The authors developed a workflow to deliver comprehensive cWGTS results within clinically-relevant timeframes. Compared to other diagnostic methods, cWGTS has the potential to capture all clinically reported mutations with comparable sensitivity. These findings provide important technical considerations for implementing cWGTS in clinical oncology.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Ze Wang, Mingming Chen, Na Liu, Yongkang Zhao, Jintao Ru, Chuanyu Qin, Tingdi Zhang
Summary: In this study, the metabolic mechanisms of testosterone and 17β-estradiol were compared by transcriptome analysis in C. testosteroni JLU460ET for the first time. The results showed that both substrates induced similar upregulated genes, but the induction level was higher for testosterone. A 100 kb steroid-degrading gene cluster and its associated genes were also revealed by transcriptome analysis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tanzeem Fatima, Rangachari Krishnan, Ashutosh Srivastava, Vageeshbabu S. Hanur, M. Srinivasa Rao
Summary: In this study, a genome-wide transcriptome analysis was conducted to identify the genes involved in high oil biosynthesis in East Indian Sandalwood. The results showed the presence of differentially expressed genes and revealed the participation of specific cytochrome families in high oil biosynthesis. These findings can contribute to the genetic improvement program for selecting superior sandalwood specimens with high oil production.
Article
Multidisciplinary Sciences
Jihye Hwang, Eun Hwa Choi, Bia Park, Gyeongmin Kim, Chorong Shin, Joon Ha Lee, Jae Sam Hwang, Ui Wook Hwang
Summary: In this study, the gene expression abundance related to wing development and metamorphosis in the white-spotted flower chafer Protaetia brevitarsis seulensis was explored based on large-scale RNA-seq data. The transcriptome assembly consisted of 23,551 high-quality transcripts with a coverage of approximately 96.7%. A total of 265 wing development genes, 19 metamorphosis genes, and 1,314 candidates were identified. Network centrality analyses revealed that wing development- and metamorphosis-related genes had a high degree of betweenness centrality. These genes were expressed most highly in eggs, moderately in pupa and adults, and lowest in larva. This study provides valuable clues for understanding the genetic modulation mechanism of wing development and metamorphosis in P. b. seulensis.
Article
Biotechnology & Applied Microbiology
Jiri Vorel, Krystyna Cwiklinski, Pavel Roudnicky, Jana Ilgova, Lucie Jedlickova, John P. Dalton, Libor Mikes, Milan Gelnar, Martin Kasny
Summary: This study presented a comprehensive transcriptomic/secretome description of Eudiplozoon nipponicum, shedding light on the physiological pathways and protein molecules involved in host-parasite interactions. By integrating high-throughput sequencing techniques, mass spectrometry analysis, and bioinformatics, the study provided a better understanding of the molecular biology and biochemistry of these often neglected organisms. The research identified numerous transcripts and proteins responsible for immunomodulation, feeding, and development, offering valuable insights into the biology of Diplozoidae ectoparasites.
Article
Endocrinology & Metabolism
Maarten C. Bosland, Katherine Vega, Lori Horton, Michael J. Schlicht
Summary: The study aimed to determine if treatment with testosterone combined with nonestrogenic estrogen metabolites is sufficient for inducing a high incidence of prostate cancer in NBL rats. The results showed that long-term treatment with a nongenotoxic estrogen and testosterone, as well as uninhibited prostatic aromatase activity generating genotoxic estrogen, are all required for the development of prostate adenocarcinomas in NBL rats.
Article
Environmental Sciences
Peng Hao, Zongshuo Lv, Sicheng Wu, Xiqing Zhang, Changlong Gou, Lixia Wang, Yanbin Zhu, Wangdui Basang, Yunhang Gao
Summary: 17-beta-estradiol (E2) pollution is a major concern due to its risks to the environment and human health. However, the mechanism of microbial degradation of E2 is not well understood. This study investigated the location of E2-degrading enzymes and conducted transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The results revealed differentially expressed genes (DEGs) related to transport, metabolism, and stress response, providing new insights into the molecular mechanism of E2 biotransformation by M. resistens MZT7.
ENVIRONMENTAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Norifusa Iwahashi, Hironobu Umakoshi, Masatoshi Ogata, Tazuru Fukumoto, Hiroki Kaneko, Eriko Terada, Shunsuke Katsuhara, Naohiro Uchida, Katsuhiko Sasaki, Maki Yokomoto-Umakoshi, Yayoi Matsuda, Ryuichi Sakamoto, Yoshihiro Ogawa
Summary: This study evaluated the utility of transcriptome data from formalin-fixed paraffin-embedded (FFPE) samples in adrenocortical tumor research. The results demonstrated that FFPE samples can serve as a reliable alternative to fresh frozen samples for whole transcriptome profiling of adrenocortical tumors, enabling distinction of tumor types and detection of marker genes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Danyel Fernandes Contiliani, Joao Felipe Carlos de Oliveira Nebo, Rafael Vasconcelos Ribeiro, Larissa Mara Andrade, Rafael Favero Peixoto Junior, Carolina Gimiliani Lembke, Ricardo Silverio Machado, Daniel Nunes Silva, Mariana Belloti, Glaucia Mendes de Souza, Dilermando Perecin, Tiago Campos Pereira, Regina Celia de Matos Pires, Patricia Rezende Fontoura, Marcos G. A. Landell, Antonio Figueira, Silvana Creste
Summary: This study compared the transcriptional profiles of two sugarcane genotypes with contrasting drought tolerance in a drought-prone environment. The results revealed differentially expressed genes related to oxidoreductase activity, transcriptional regulation, amino acid metabolism, and translation, with a greater number of exclusive transcripts in the drought-tolerant genotype. The enhanced antioxidant system of the tolerant genotype protects photosystem II from oxidative damage, ensuring stable photochemical activity even after prolonged water shortage. Additionally, the tolerant genotype exhibits a more extensive set of responsive transcription factors, promoting the fine-tuning of drought-related molecular pathways. These findings provide insights into the molecular mechanisms of drought tolerance in sugarcane and have potential applications in plant breeding programs.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Marianna Tyczewska, Marta Szyszka, Karol Jopek, Marcin Rucinski
Summary: The expression changes of peptides and their receptors, as well as steroidogenesis enzymes, were investigated in the hypothalamus, pituitary, and adrenal glands of rats after intraperitoneal administration of peptides. The results showed significant expression changes in the hypothalamus and pituitary after 1-hour exposure to peptides, including a decrease in corticotropin-releasing hormone expression and a decrease in the expression of galanin receptors. The expression of steroidogenesis enzymes also changed, with an increase in cholesterol desmolase expression after peptide administration.
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Oncology
Igor Piotrowski, Katarzyna Kulcenty, Wiktoria Suchorska, Marcin Rucinski, Karol Jopek, Marta Kruszyna-Mochalska, Agnieszka Skrobala, Piotr Romanski, Adam Ryczkowski, Dorota Borowicz, Natalia Matuszak, Julian Malicki
Summary: This study investigated the effects of hypo-fractionated stereotactic body radiation therapy (SBRT) on out-of-field organs in prostate cancer treatment. The results showed higher radiation doses in the intestine and lung, with a small increase in DNA damage and cell killing in the intestines. Gene expression analysis revealed enrichment of radiation response-related biological processes in the prostate, but no clear changes in relevant processes in the lung and thyroid.
Article
Cell Biology
Iwona Mazur-Michalek, Marcin Rucinski, Mateusz Sowinski, Paulina Pietras, Marta Lesniczak-Staszak, Witold Szaflarski, Mark Isalan, Michal Mielcarek
Summary: This study identifies transcriptional biomarkers for Huntington's disease and reveals deregulated networks related to visual processes and muscle contractions. Some of the deregulated genes may be linked to other genetic ocular disorders.
Article
Genetics & Heredity
Mariusz J. Nawrocki, Karol Jopek, Mariusz Kaczmarek, Maciej Zdun, Paul Mozdziak, Marek Jemielity, Dorota Bukowska, Bartosz Kempisty, Bartlomiej Perek
Summary: Numerous cardiovascular diseases can lead to severe myocardial dysfunction, which is a common cause of death globally. Understanding the molecular mechanisms of these diseases is essential for developing effective therapies. In this study, the transcriptomic profile of atrial cardiomyocytes during long-term primary cell culture was analyzed, revealing changes in gene expression patterns. It was found that certain genes related to cellular architecture were downregulated during in vitro culture, potentially affecting the formation of intracellular structures.
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marianna Tyczewska, Patrycja Sujka-Kordowska, Marta Szyszka, Karol Jopek, Malgorzata Blatkiewicz, Ludwik K. Malendowicz, Marcin Rucinski
Summary: The homeostasis of the adrenal gland is crucial for its proper functioning in both non-stressful and stressful conditions, involving interactions between different types of cells. However, there is insufficient information regarding marker gene expression in the rat adrenal gland under non-stressful conditions. This study aimed to determine the expression of marker genes in different zones of the rat adrenal gland and revealed distinct expression patterns of interstitial cell markers in specific zones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Rut Bryl, Mariusz J. Nawrocki, Karol Jopek, Mariusz Kaczmarek, Dorota Bukowska, Pawel Antosik, Paul Mozdziak, Maciej Zabel, Piotr Dziegiel, Bartosz Kempisty
Summary: Heart failure is a leading cause of death globally, and current treatment options are often not sufficient. Autologous stem cell transplant shows promise as an alternative approach in clinical management. Studies have revealed differentially expressed genes in cell cultures from the heart, suggesting the involvement of stem cell maintenance and proliferation in myocardial regeneration.
Article
Biochemistry & Molecular Biology
Malgorzata Blatkiewicz, Kacper Kaminski, Marta Szyszka, Zaid Al-Shakarchi, Anna Olechnowicz, Ewelina Stelcer, Hanna Komarowska, Marianna Tyczewska, Anna Klimont, Marek Karczewski, Tomasz Wierzbicki, Joanna Mikolajczyk-Stecyna, Marek Ruchala, Ludwik K. Malendowicz, Marcin Rucinski
Summary: The study examines the association of ZWILCH gene with adrenocortical carcinoma (ACC). The higher expression of ZWILCH gene was found in ACC tissue compared to normal adrenal glands. Upregulation of ZWILCH is correlated with tumor mitotic rate and patient survival. The enhanced ZWILCH level is also associated with activation of genes involved in cell proliferation and inhibition of genes related to the immune system.
Article
Biochemistry & Molecular Biology
Remigiusz Domin, Michal Pytka, Mikolaj Zolynski, Jan Nizinski, Marcin Rucinski, Przemyslaw Guzik, Jacek Zielinski, Marek Ruchala
Summary: This study aimed to investigate the relationship between serum MOTS-c concentration and muscle strength parameters, as well as oxygen consumption, in physically active individuals. The results showed that higher serum MOTS-c concentration was associated with greater muscle mass, force, and power, but not with exercise capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalia Izycka, Marcin Rucinski, Malgorzata Andrzejewska, Sebastian Szubert, Ewa Nowak-Markwitz, Karolina Sterzynska
Summary: Recurrent disease and treatment-associated chemoresistance are major contributors to poor clinical outcomes in ovarian cancer patients. This study investigated the expression of CSC-associated markers ALDH1A, CD44, and CD133 to predict patient prognosis. ALDH1A1-positive patients had significantly shorter overall survival (OS) and progression-free survival (PFS). Multivariate analysis revealed that ALDH1A1 expression, FIGO stage III-IV, and residual disease were associated with shorter OS. Targeting ALDH1A1-positive cancer cells may be a promising therapeutic strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Anna Rawluszko-Wieczorek, Julia Lipowicz, Marta Nowacka, Kamila Ostrowska, Paulina Pietras, Malgorzata Blatkiewicz, Marcin Rucinski, Pawel Piotr Jagodzinski, Michal Nowicki
Summary: The presence of estrogen receptor beta (ER beta) is inversely correlated with the occurrence of colorectal cancer (CRC), and low expression of ER beta is associated with poorer overall survival of CRC patients. ER beta can inhibit tumorigenesis through various molecular pathways and also has an impact on the hypoxic response in CRC cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)