Article
Infectious Diseases
Reena Lamichhane-Khadka, Santosh Dulal, Jesus A. Cuaron, Richard Pfeltz, Sushim Kumar Gupta, Brian J. Wilkinson, John E. Gustafson
Summary: Comparative genomic sequencing of two laboratory-derived vancomycin-intermediate Staphylococcus aureus (VISA) strains (MM66-3 and MM66-4) revealed unique mutations compared to the parent strain MM66. Transcriptional profiling showed similar changes in both VISA strains, including reduced autolysis, growth in the presence of salt, and virulence factor activity. Mutations in the key gene apt may play a role in altered adenine metabolism in VISA strains.
Article
Biochemical Research Methods
Yu-Ching Liu, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chuan Lin, Chao-Jung Chen
Summary: The rapid identification of methicillin-sensitive Staphylococcus aureus (MSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-intermediate S. aureus (VISA) is crucial for accurate treatment, timely intervention, and outbreak prevention. Proteomics analysis of 90 S. aureus isolates led to the discovery of protein biomarkers that can discriminate among MSSA, hVISA, and VISA strains, with two VISA, two hVISA, and one MSSA protein biomarkers identified and verified for high sensitivities and specificities. Data is available via MassIVE with identifier MSV000085776.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Microbiology
Robby Markwart, Niklas Willrich, Tim Eckmanns, Guido Werner, Olaniyi Ayobami
Summary: The study found that resistance to linezolid and daptomycin in VREF and MRSA between 2014 and 2018 was relatively low, indicating the continued effectiveness of these last-resort antibiotics in Europe. However, efforts to monitor and contain antimicrobial resistance should be strengthened at regional and national levels.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Siheng Wang, Chang Cai, Yingbo Shen, Chengtao Sun, Qingxin Shi, Ningjun Wu, Shufang Zheng, Jiao Qian, Rong Zhang, Hongwei Zhou
Summary: Contezolid exhibits potent activity against MRSA and VRE strains. It shows similar or slightly better antimicrobial activity compared to linezolid. Contezolid may have potential benefits for treating infections caused by MRSA and VRE pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Alina Dietrich, Ursula Steffens, Mike Gajdiss, Anna-Lena Boschert, Jana Katharina Droege, Christiane Szekat, Peter Sass, Imran T. Malik, Jan Bornikoel, Laura Reinke, Boris Macek, Mirita Franz-Wachtel, Kay Nieselt, Theresa Harbig, Kirstin Scherlach, Heike Broetz-Oesterhelt, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Summary: Resistance to antibiotics is a growing problem and alternative antimicrobial therapies are needed. Cervimycin, a polyketide antibiotic, shows promising activity against multidrug-resistant staphylococci and vancomycin-resistant enterococci. This study identified the Clp system and the essential kinase WalK as mutational hotspots for cervimycin resistance in Staphylococcus aureus. Furthermore, cervimycin-resistant strains exhibited vancomycin-intermediate resistance phenotype, suggesting a role of cervimycin in perturbing the bacterial cell envelope.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shawna McCallin, Carmen Menzi, Swenja Lassen, Jean Daraspe, Frank Oechslin, Philippe Moreillon
Summary: In Vancomycin-intermediate Staphylococcus aureus (VISA), exposure to vancomycin may lead to cross-resistance to phage. VISA strains with intermediate levels of vancomycin resistance showed decreased sensitivity to phage. Serial passaging with vancomycin induced reduced vancomycin susceptibility and phage sensitivity. Understanding the interference between antibiotic and phage resistance mechanisms is important for designing effective therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Samantha D. Yeager, Justin E. Oliver, Mahmoud A. Shorman, Laurence R. Wright, Michael P. Veve
Summary: This study compared the effectiveness and safety of oral step-down linezolid with standard parenteral therapy in MRSA bloodstream infection. The results showed that linezolid had similar clinical outcomes and safety profiles compared to parenteral therapy, with fewer adverse events requiring re-hospitalization. Additional research is needed to explore the use of step-down linezolid in MRSA bloodstream infection.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Javier Campanini-Salinas, Juan Andrades-Lagos, Nicolas Hinojosa, Fabian Moreno, Pedro Alarcon, Gerardo Gonzalez-Rocha, Ian E. Burbulis, David Vasquez-Velasquez
Summary: New quinone compounds show promising inhibitory activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus, including clinical isolates. The compounds did not induce resistance in the clinical strains and were as effective as vancomycin in protecting larvae from MRSA infection. These quinone derivatives have potential as drug candidates for further development.
Article
Infectious Diseases
Hideo Kato, Mao Hagihara, Nobuhiro Asai, Yuichi Shibata, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
Summary: This study found through a meta-analysis of RCTs and CSs that LZD has significantly higher clinical cure and microbiological eradication rates compared to VCM in the treatment of proven MRSA pneumonia. However, there was no significant difference in mortality and adverse events between VCM and LZD.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Grace Kaul, Abdul Akhir, Manjulika Shukla, Kundan S. Rawat, Chandra P. Sharma, Komal G. Sangu, Haridas B. Rode, Atul Goel, Sidharth Chopra
Summary: Nitazoxanide exhibits potent activity against clinical drug-resistant S. aureus and Enterococcus spp., concentration-dependent killing, ability to eradicate S. aureus biofilm, and lack of generation of resistance in S. aureus. Nitazoxanide synergizes strongly with linezolid against linezolid-susceptible and -resistant S. aureus, showing superior activity compared to untreated control and drug-alone treatment groups, both in vitro and in vivo.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Shuaishuai Wang, Huiping Liu, Jun Mao, Yu Peng, Yisong Yan, Yaowen Li, Na Zhang, Lifang Jiang, Yanyan Liu, Jiabin Li, Xiaohui Huang
Summary: The combination of linezolid and fosfomycin shows promise in treating severe infections caused by VREfm, producing sustained bactericidal effects and eradicating resistant subpopulations. The study also highlights the relationship between fosfomycin resistance and bacterial virulence in VREfm, showing that the development of fosfomycin resistance may come at the expense of bacterial virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Multidisciplinary Sciences
Pooi Yin Chung, Pey Lin Narissa Loh, Hui-min Neoh, Ramliza Ramli
Summary: Staphylococcus aureus is a significant threat to human health and has developed resistance to conventional antimicrobials. The presence of biofilms complicates treatment for infections caused by multi-drug resistant S. aureus. Alpha-amyrin has been shown to reduce biomass and inhibit metabolic activity of S. aureus. This study demonstrates that alpha-amyrin can reduce biofilm biomass of MSSA, MRSA, and VISA isolates, suggesting its potential as a therapeutic agent for chronic S. aureus infections.
Article
Engineering, Biomedical
Mariam Taha, Rozanne Arulanandam, Andrew Chen, Jean-Simon Diallo, Hesham Abdelbary
Summary: There is controversial clinical evidence regarding the added antibacterial benefit of locally administering antiseptic solutions or antibiotics to the infected joint space. The objectives of this in vitro study were to test the efficacy of povidone-iodine (PVP-I) and vancomycin in treating premature and developed Staphylococcus aureus biofilms grown on titanium implant surfaces. The combination of PVP-I and vancomycin is superior in reducing viable S. aureus cells in immature biofilms grown on titanium surface without causing significant cytotoxicity to muscle tissue.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Infectious Diseases
Yanlei Xu, Bingjie Wang, Huilin Zhao, Xinyi Wang, Lulin Rao, Wenxiu Ai, Jingyi Yu, Yinjuan Guo, Xiaocui Wu, Fangyou Yu, Shuying Chen
Summary: The study found that all MRSA isolates remained sensitive to vancomycin, teicoplanin, linezolid and daptomycin, but an increase in MIC50 and MIC90 was observed for linezolid.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Surgery
Francisco Lopez-Medrano, Jose Tiago Silva, Mario Fernandez-Ruiz, Elisa Vidal, Julia Origuen, Antonia Calvo-Cano, Enrique Luna-Huerta, Esperanza Merino, Domingo Hernandez, Cristina Jironda-Gallegos, Rosa Escudero, Francesca Gioia, Antonio Moreno, Cristina Roca, Elisa Cordero, Dario Janeiro, Beatriz Sanchez-Sobrino, Maria Milagro Montero, Dolores Redondo, Francisco Javier Candel, Isabel Perez-Flores, Carlos Arminanzas, Claudia Gonzalez-Rico, Maria Carmen Farinas, Emilio Rodrigo, Belen Loeches, Maria O. Lopez-Oliva, Miguel Montejo, Ricardo Lauzurica, Juan Pablo Horcajada, Julio Pascual, Amado Andres, Jose Maria Aguado
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Article
Microbiology
Francisco Javier Candel Gonzalez, Jose Manuel Vinuela-Prieto, Juan Gonzalez del Castillo, Pablo Barreiro Garcia, Marcos Fragiel Saavedra, Alba Hernandez Piriz, David Jimenez Virumbrales, Jesus Canora Lebrato, Gonzalo Garcia de Casasola, Ruth Gil Prieto, Jesus San-Roman Montero, Justo Ortega Anselmi, Raquel Barba Martin, Fernando Prados Roa, Javier Marco Martinez, Antonio Zapatero Gaviria
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2020)
Article
Geriatrics & Gerontology
Francisco Javier Candel, Pablo Barreiro, Jesus San Roman, Maria del Mar Carretero, Juan Carlos Sanz, Marta Perez-Abeledo, Belen Ramos, Jose Manuel Vinuela-Prieto, Jesus Canora, Francisco Javier Martinez-Peromingo, Raquel Barba, Antonio Zapatero
Summary: The study highlights the high prevalence of COVID-19 among residents in nursing homes, especially those who are frail and have comorbidities. Factors associated with seropositivity among staff include age, obesity, job type, workplace seroprevalence, and contact with external COVID-19 cases. Strengthening control measures is crucial to prevent COVID-19 transmission in care facilities.
Article
Virology
Pablo Barreiro, Francisco Javier Candel, Juan Carlos Sanz, Jesus San Roman, Maria del Mar Carretero, Marta Perez-Abeledo, Belen Ramos, Jose Manuel Vinuela-Prieto, Jesus Canora, Francisco Javier Martinez-Peromingo, Antonio Zapatero
Summary: The study examined the virological significance of different serological patterns in asymptomatic COVID-19 individuals, finding that the combination of IgM-S and IgG-N is important for detecting SARS-CoV-2 infection. The occurrence of IgM-S(+) alone reactivity is rare, and only a small proportion is associated with viral shedding.
Article
Emergency Medicine
Maria L. Gandia-Gonzalez, Jose M. Vinuela-Prieto, Laura Barrios, Carlos Alarcon, Fuat Arikan, Cinta Arraez, Carlos J. Dominguez, Jose F. Alen, Raquel Gutierrez-Gonzalez, Angel Horcajadas, Fernando Munoz Hernandez, Alejandra Narvaez, Igor Paredes, Rebeca Perez-Alfayate, Angel Rodriguez de Lope, Fernando Ruiz-Juretschke, Freddy J. Salge Arrieta, Sonia Tejada, Martin Tamarit, Thomaz Topczewski, Jesus Lafuente
Summary: The study evaluated the impact of COVID-19 outbreak on the number of neurosurgical emergencies in different regions of Spain. It was found that the most affected regions experienced a significant drop in the need for emergency surgeries, while the number of life-threatening patients did not decrease.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Immunology
Jose Manuel Vinuela-Prieto, Maria Carmen Escarpa Falcon, Francisco Javier Candel, Alonso Mateos Rodriguez, Juan Ignacio Torres Gonzalez, Francisco del Rio Gallegos
Summary: This study found that factors such as interviewer experience, donation after brain death, number of interviews conducted per family, age of the main decision maker, and cultural levels of the family were independently associated with family refusal to consent to donation. The logistic regression model displayed good predictive power for both derivation and validation cohorts, with an overall predictive accuracy of 80.9% and 74.4%, respectively.
TRANSPLANTATION PROCEEDINGS
(2021)
Article
Environmental Sciences
Antonio Lastra, Jaime Botello, Alejandro Pinilla, Joseba Inaki Urrutia, Jesus Canora, Juan Sanchez, Pascual Fernandez, Francisco Javier Candel, Antonio Zapatero, Monica Ortega, Jaime Flores
Summary: This study aims to share the methodology and implementation of SARS-CoV-2 detection in wastewater in the Madrid region, with weekly testing of 289 sampling points to provide data support for early warning of the COVID-19 pandemic.
ENVIRONMENTAL RESEARCH
(2022)
Article
Clinical Neurology
Victor E. Staartjes, Vittorio Stumpo, Luca Ricciardi, Nicolai Maldaner, Hubert A. J. Eversdijk, Moira Vieli, Olga Ciobanu-Caraus, Antonino Raco, Massimo Miscusi, Andrea Perna, Luca Proietti, Giorgio Lofrese, Michele Dughiero, Francesco Cultrera, Nicola Nicassio, Seong Bae An, Yoon Ha, Aymeric Amelot, Irene Alcobendas, Jose M. Vinuela-Prieto, Maria L. Gandia-Gonzalez, Pierre-Pascal Girod, Sara Lener, Nikolaus Koegl, Anto Abramovic, Nico Akhavan Safa, Christoph J. Laux, Mazda Farshad, Dave O'Riordan, Markus Loibl, Anne F. Mannion, Alba Scerrati, Granit Molliqaj, Enrico Tessitore, Marc L. Schroeder, W. Peter Vandertop, Martin N. Stienen, Luca Regli, Carlo Serra
Summary: This study developed clinical prediction models for long-term functional impairment, back pain, and leg pain after lumbar fusion for degenerative disease. The models showed moderate ability to identify patients who are likely to benefit from surgery. However, more robust tools are needed to predict outcomes in this patient population in the era of personalized medicine.
EUROPEAN SPINE JOURNAL
(2022)
Article
Microbiology
Pablo Barreiro, Juan Carlos Sanz, Jesus San Roman, Marta Perez-Abeledo, Mar Carretero, Gregoria Megias, Jose Manuel Vinuela-Prieto, Belen Ramos, Jesus Canora, Francisco Javier Martinez-Peromingo, Raquel Barba, Antonio Zapatero, Francisco Javier Candel
Summary: Assessing T-cell responses to SARS-CoV-2 antigens is important for determining long-lasting protection against breakthrough infections or reinfections. The interferon gamma release assay, a validated method for testing cellular immunity in mycobacterial infections, has been proposed for patients with SARS-CoV-2 infection or vaccination.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Marina Penuelas, Cristina Garcia-Salguero, Melania Inigo, Jose Manuel Vinuela-Prieto, Francisco Javier Candel, Jose Luis del Pozos, Esther Culebras
Summary: The study found that the IMI/REL combination has high activity against bacteria carrying KPC enzymes, while there was no change in activity against P. aeruginosa and A. baumannii strains carrying VIM enzyme. In P. aeruginosa strains without carbapenemase, the MIC for IMI/REL was reduced.
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2021)
Article
Immunology
Jesus San Roman, Francisco Javier Candel, Juan Carlos Sanz, Paloma Lopez, Rocio Menendez-Colino, Pablo Barreiro, Maria del Mar Carretero, Marta Perez-Abeledo, Jose Manuel Vinuela-Prieto, Belen Ramos, Jesus Canora, Raquel Barba, Antonio Zapatero-Gaviria, Franciso Javier Martinez-Peromingo
Summary: This study analyzed the immune response of residents and staff in nursing homes after receiving the COVID-19 vaccine. The study found that younger age and prior COVID-19 infection were associated with stronger humoral immune response after vaccination. However, the immune response may wane over time and reinfection seems to be more frequent in older adults.
Article
Virology
Fernando Cava, Jesus San Roman, Pablo Barreiro, Francisco Javier Candel, Francisco Javier Alvarez-Timon, David Melero, Nerea Coya, Raquel Guillen, David Cantarero-Prieto, Javier Lera-Torres, Noelia Cobo-Ortiz, Jesus Canora, Francisco Javier Martinez-Peromingo, Raquel Barba, Maria del Mar Carretero, Juan Emilio Losa, Antonio Zapatero
Summary: Prediction tools can help guide public health decisions by forecasting COVID-19 case surges and hospitalizations. Low cycle threshold (CT) counts can indicate a higher concentration of SARS-CoV-2 in the respiratory tract, serving as a marker for increased viral transmission. This study validated CT counts as an epidemiological marker for predicting pandemic waves, showing a temporal association between CT counts and COVID-19 cases and hospital admissions.
Review
Medicine, General & Internal
Francisco Javier Candel, Miguel Salavert, Miren Basaras, Marcio Borges, Rafael Canton, Emilia Cercenado, Catian Cilloniz, Angel Estella, Juan M. Garcia-Lechuz, Jose Garnacho Montero, Federico Gordo, Agustin Julian-Jimenez, Francisco Javier Martin-Sanchez, Emilio Maseda, Mayra Matesanz, Rosario Menendez, Manuel Miron-Rubio, Raul Ortiz de Lejarazu, Eva Polverino, Pilar Retamar-Gentil, Luis Alberto Ruiz-Iturriaga, Susana Sancho, Leyre Serrano
Summary: This paper reviews important issues in the approach to community-acquired pneumonia, including updates in etiology, diagnostic guidance, treatment, management, and prevention.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Infectious Diseases
Carlota Gudiol, Manuela Aguilar-Guisado, Jose Ramon Azanza, Francisco Javier Candel, Rafael Canton, Jordi Carratala, Carolina Garcia-Vidal, Isidro Jarque, Manuel Lizasoain, Jose Molina Gil-Bermejo, Isabel Ruiz-Camps, Isabel Sanchez-Ortega, Carlos Solano, Maria Suarez-Lledo, Lourdes Vazquez, Rafael de la Camara
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2020)