Article
Cell Biology
Abdellatif Elseoudi, Takashi Nishida, Tomomi Mizukawa, Takako Hattori, Kazumi Kawata, Eman A. Taha, Masaharu Takigawa, Satoshi Kubota
Summary: FGF-1, in addition to inducing cell proliferation, also acts as a catabolic molecule in chondrocytes, repressing CCN2 and amplifying its own production through positive feedback regulation. The regulation of CCN2 repression and FGF1 induction is affected by HDAC1, while FOXA1 plays a key role as a transcription factor in this process.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2021)
Article
Multidisciplinary Sciences
Alireza Savadipour, Robert J. Nims, Neda Rashidi, Jaquelin M. Garcia- Castorena, Ruhang Tang, Gabrielle K. Marushack, Sara J. Oswald, Wolfgang B. Liedtke, Farshid Guilak
Summary: Through AFM and FEM studies, we found that PIEZO1 and PIEZO2 mediate calcium (Ca2+) signaling in chondrocytes at moderately high levels of cellular deformation, while at the highest strains, PIEZO1 functions independently of PIEZO2. Biophysical factors that increase apparent chondrocyte membrane tension also enhance PIEZO1-driven Ca2+ signaling. We identified a threshold of membrane stretch ratio that leads to PIEZO1-induced chondrocyte mechanical injury, suggesting potential targets for osteoarthritis prevention or treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Priyanka Kothari, Ashish Kumar Tripathi, Aboli Girme, Divya Rai, Ruchi Singh, Shradha Sinha, Dharmendra Choudhary, Geet Kumar Nagar, Rakesh Maurya, Lal Hingorani, Ritu Trivedi
Summary: DS extract showed protective effects on joint cartilage and subchondral bone deterioration in vivo, while CAFG inhibited the effects induced by IL-1 beta and could be a potential therapeutic for treating osteoarthritis.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Cell & Tissue Engineering
Zhuoxuan Jia, Bijun Kang, Yizuo Cai, Chingyu Chen, Zheyuan Yu, Wei Li, Wenjie Zhang
Summary: The study found that CEFFE (a cell-free fat extract containing multiple cytokines) inhibits the progression of osteoarthritis by promoting cartilage regeneration and limiting joint inflammation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Alice Ramesova, Barbora Vesela, Eva Svandova, Herve Lesot, Eva Matalova
Summary: The study reveals new functions of Caspase-1 in chondrocyte differentiation and lipid metabolism pathways, showing that inhibiting Caspase-1 affects the expression of Cd36 and Pparg, impacting chondrogenic nodule formation. Additionally, the downregulation of Cd36 affects Rankl and Opg oppositely to the inhibition of Caspase-1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
K. S. M. Reed, V. Ulici, C. Kim, S. Chubinskaya, R. F. Loeser, D. H. Phanstiel
Summary: Treatment of normal human articular chondrocytes with fibronectin fragments can recapitulate key aspects of the osteoarthritis chondrocyte phenotype, showing promising potential for future osteoarthritis studies, especially considering its compatibility with genomics and genome-editing techniques.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Biochemistry & Molecular Biology
Carmen Nunez-Carro, Margarita Blanco-Blanco, Tatiana Montoya, Karla M. Villagran-Andrade, Tamara Hermida-Gomez, Francisco J. Blanco, Maria C. de Andres
Summary: Osteoarthritis is a chronic disease that affects articular cartilage and leads to its degeneration. Recent research has shown the importance of the interplay between inflammatory processes and epigenetic regulation in the development of osteoarthritis. However, the lack of specific protocols to extract histones from human articular cartilage has hindered further investigation in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xianming Wang, Yu Cai, Cuixi Wu, Jiamin Liang, Kangning Tang, Zefeng Lin, Lingling Chen, Yao Lu, Qing Wang
Summary: The study demonstrates the use of functionalized small copper sulfide nanoparticles (B2M-CuS NPs) targeted at senescent chondrocytes for the treatment of osteoarthritis. These nanoparticles catalyze the formation of toxic •OH from H2O2 via peroxidase-like activity, effectively clearing senescent cells and promoting cartilage regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Integrative & Complementary Medicine
Zhen Li, Zhenyue Chen, Jiayi Chen, Zhutong Liu, Zehui Li, He Sun, Xiaochao Wang, Jinqiang Wei, Xuewei Cao, Decai Zheng
Summary: This study found that monotropein (MON), the main active ingredient of Morinda officinalis F.C. How, has therapeutic effects on chondrocytes and a mouse model of OA. It alleviates OA progression by inhibiting cartilage matrix degradation, apoptosis, and pyroptosis of chondrocytes through NF-kappa B pathway inactivation. MON shows promise as an alternative treatment for OA.
Article
Pharmacology & Pharmacy
Jiaqin Wu, Yuna Qian, Cheng Chen, Fan Feng, Lianhong Pan, Li Yang, Chunli Wang
Summary: This study demonstrated that Hesperetin (HPT) has chondroprotective and anti-inflammatory effects on chondrocytes through the AMPK signaling pathway, inhibiting TNF-alpha-induced cartilage degradation and promoting chondrocyte proliferation. RNA sequencing identified potential molecular targets, with 549 differentially expressed genes, and highlighted the importance of the AMPK pathway in mediating HPT's protective effects. Overall, HPT shows promise as a natural drug for osteoarthritis treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Rheumatology
Caining Wen, Limei Xu, Xiao Xu, Daping Wang, Yujie Liang, Li Duan
Summary: IGF-1 plays a crucial role in promoting chondrocyte proliferation and matrix production, while inhibiting chondrocyte apoptosis in articular cartilage repair. Optimizing IGF delivery systems can enhance the efficacy of cartilage repair treatments.
ARTHRITIS RESEARCH & THERAPY
(2021)
Article
Orthopedics
Zhimin Wu, Ying Wang, Guoqiang Yan, Chengai Wu
Summary: Eugenol has been shown to have anti-inflammatory, antioxidant, and antiapoptotic effects in multiple systems and organs of the human body. This study demonstrates its therapeutic potential for osteoarthritis (OA) by protecting cartilage and subchondral bone, inhibiting inflammatory molecules, and blocking IL-1β-induced signaling pathways.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biology
Yuchen He, Katelyn E. Lipa, Peter G. Alexander, Karen L. Clark, Hang Lin
Summary: Osteoarthritis (OA) is a common degenerative joint disease with no effective treatment currently available. This review examines the similarities between OA chondrocytes and aging cells, and proposes potential methods to reverse the osteoarthritic phenotype. The challenges and future perspectives in OA research are also discussed.
Article
Plant Sciences
Huan Yu, Sai Yao, Chengchong Zhou, Fangda Fu, Huan Luo, Weibin Du, Hongting Jin, Peijian Tong, Di Chen, Chengliang Wu, Hongfeng Ruan
Summary: The study demonstrated that morroniside can attenuate the progression of osteoarthritis in mice by promoting cartilage matrix synthesis, inhibiting chondrocyte pyroptosis, reducing osteophyte formation, and subchondral sclerosis.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Oncology
Chiharu Sogawa, Takanori Eguchi, Manh Tien Tran, Masayuki Ishige, Kilian Trin, Yuka Okusha, Eman Ahmed Taha, Yanyin Lu, Hotaka Kawai, Norio Sogawa, Masaharu Takigawa, Stuart K. Calderwood, Kuniaki Okamoto, Ken-ichi Kozaki
Article
Oncology
Eman A. Taha, Chiharu Sogawa, Yuka Okusha, Hotaka Kawai, May Wathone Oo, Abdellatif Elseoudi, Yanyin Lu, Hitoshi Nagatsuka, Satoshi Kubota, Ayano Satoh, Kuniaki Okamoto, Takanori Eguchi
Article
Oncology
Yuka Okusha, Takanori Eguchi, Manh T. Tran, Chiharu Sogawa, Kaya Yoshida, Mami Itagaki, Eman A. Taha, Kisho Ono, Eriko Aoyama, Hirohiko Okamura, Ken-ichi Kozaki, Stuart K. Calderwood, Masaharu Takigawa, Kuniaki Okamoto
Article
Cell Biology
Takanori Eguchi, Chiharu Sogawa, Kisho Ono, Masaki Matsumoto, Manh Tien Tran, Yuka Okusha, Benjamin J. Lang, Kuniaki Okamoto, Stuart K. Calderwood
Article
Biochemistry & Molecular Biology
Mariko Seyama, Kaya Yoshida, Kayo Yoshida, Natsumi Fujiwara, Kisho Ono, Takanori Eguchi, Hotaka Kawai, Jiajie Guo, Yao Weng, Yuan Haoze, Kenta Uchibe, Mika Ikegame, Akira Sasaki, Hitoshi Nagatsuka, Kuniaki Okamoto, Hirohiko Okamura, Kazumi Ozaki
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Manh Tien Tran, Yuka Okusha, Yunxia Feng, Masatoshi Morimatsu, Penggong Wei, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Hirohiko Okamura, Keiji Naruse, Takayuki Tsukuba, Kuniaki Okamoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Editorial Material
Medicine, General & Internal
Takanori Eguchi
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Cell Biology
Chiharu Sogawa, Takanori Eguchi, Yuri Namba, Yuka Okusha, Eriko Aoyama, Kazumi Ohyama, Kuniaki Okamoto
Summary: Research has shown that tumoroids of metastatic colorectal cancer exhibit different responses to cisplatin, imatinib, and 5-fluorouracil in a 3D culture system, indicating that this system is useful for modeling drug resistance in cancer with enhanced stem cell properties.
Article
Health Care Sciences & Services
Chi Ngai Lo, Tarek Abdelkader, Yik Ming Choi, Anthony James Goff, Krishnasamy Suresh, Guiller Augustin Cea Carpio, Benjamin Soon
Summary: This study compares the effectiveness of 3D images produced by photogrammetry and traditional 2D images in introducing manual therapy skills to physiotherapy students. The results show that for learning three cervical spine assessment skills, 3D images produced by photogrammetry are more effective than 2D images for first-year physiotherapy students.
SIMULATION IN HEALTHCARE-JOURNAL OF THE SOCIETY FOR SIMULATION IN HEALTHCARE
(2022)
Article
Cell Biology
Yanyin Lu, Takanori Eguchi, Chiharu Sogawa, Eman A. Taha, Manh Tien Tran, Toshiki Nara, Penggong Wei, Shiro Fukuoka, Takuya Miyawaki, Kuniaki Okamoto
Summary: Researchers developed a combination method to characterize different types of EVs and identified five types of EVs in the culture supernatant of macrophage-like cells. Through molecular transfer assays, it was confirmed that macrophage-secreted EXO-S and EXO-L were more efficient in transferring GFP to oral carcinoma cells, but also significantly reduced the cell viability of oral carcinoma cells.
Article
Cell Biology
Takanori Eguchi, Eva Csizmadia, Hotaka Kawai, Mona Sheta, Kunihiro Yoshida, Thomas L. Prince, Barbara Wegiel, Stuart K. Calderwood
Summary: The prostate epithelial transcription factor SCAND1 can reverse the mesenchymal and hybrid E/M phenotypes of cancer cells, inhibit their proliferation and migration, and promote an epithelial phenotype. SCAND1 achieves this by repressing the gene expression of EMT drivers and the MAP3K-MEK-ERK signaling pathway. Overexpression of SCAND1 has shown potential therapeutic value in reducing tumor cell proliferation and metastasis.
Review
Biology
Mona Sheta, Eman A. Taha, Yanyin Lu, Takanori Eguchi
Summary: Extracellular vesicles (EVs) are cell-derived membrane-surrounded vesicles that carry bioactive molecules and deliver them to recipient cells. They can be classified into classical EVs (exosomes, microvesicles, and apoptotic bodies) and additional types (autophagic EVs, matrix vesicles, and stressed EVs). EVs are involved in the communication between cancer cells and tumor-associated cells (TAC), leading to the establishment of immunosuppressive and chemoresistant microenvironments. Cancer EVs play important roles in immunosuppression, immune evasion, and cellular transformation, and can change hot tumors into cold ones.
Article
Cell Biology
Kisho Ono, Chiharu Sogawa, Hotaka Kawai, Manh Tien Tran, Eman A. Taha, Yanyin Lu, May Wathone Oo, Yuka Okusha, Hirohiko Okamura, Soichiro Ibaragi, Masaharu Takigawa, Ken-Ichi Kozaki, Hitoshi Nagatsuka, Akira Sasaki, Kuniaki Okamoto, Stuart K. Calderwood, Takanori Eguchi
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Article
Cell Biology
Yuka Okusha, Manh Tien Tran, Mami Itagaki, Chiharu Sogawa, Takanori Eguchi, Tatsuo Okui, Tomoko Kadowaki, Eiko Sakai, Takayuki Tsukuba, Kuniaki Okamoto
Review
Biology
Takanori Eguchi, Eman Ahmed Taha, Stuart K. Calderwood, Kisho Ono