Article
Multidisciplinary Sciences
Uxue Ulanga, Matthew Russell, Stefano Patassini, Julie Brazzatti, Ciaren Graham, Anthony D. Whetton, Robert L. J. Graham
Summary: Murine models are widely used in biology and pathology research, and targeted quantitative proteomic analysis is a new tool. SWATH-MS technique requires prior acquisition of mass spectra to generate an assay reference library. Inducing pluripotency from fibroblast population leads to major changes in expressed proteins, and a reference library has been created to support such studies.
Article
Cell Biology
Ahmad Salti, Solmaz Etemad, Marta Suarez Cubero, Eva Albertini, Beata Kovacs-Szalka, Max Holzknecht, Elia Cappuccio, Maria Cavinato, Frank Edenhofer, Pidder Jansen Durr
Summary: By studying the role of mitochondria in cellular reprogramming processes, it was found that FAHD1 plays an important role in regulating mitochondrial function, potentially acting as a negative regulator of cell reprogramming.
Article
Multidisciplinary Sciences
Lucy LeBlanc, Mijeong Kim, Aparna Kambhampati, Albert J. Son, Nereida Ramirez, Jonghwan Kim
Summary: Cell density impacts gene expression and differentiation in mouse embryonic stem cells; β-catenin is primarily at adherens junctions at high density but translocates to the nucleus at low density; Esrrb maintains pluripotency gene expression at high density and decreases DNA association at low density.
Article
Biochemistry & Molecular Biology
Fabiana Passaro, Ilaria De Martino, Federico Zambelli, Giorgia Di Benedetto, Matteo Barbato, Anna Maria D'Erchia, Caterina Manzari, Graziano Pesole, Margherita Mutarelli, Davide Cacchiarelli, Dario Antonini, Silvia Parisi, Tommaso Russo
Summary: This study reveals a new role for YAP in governing the epigenetic dynamics of pluripotency exit. Transient downregulation of YAP leads to genome-wide alteration of DNA methylation remodeling during ESC differentiation, potentially involving the regulation of molecules such as RNA ephemeron and Dnmt3l.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Meghali Aich, Asgar Hussain Ansari, Li Ding, Vytautas Iesmantavicius, Deepanjan Paul, Chunaram Choudhary, Souvik Maiti, Frank Buchholz, Debojyoti Chakraborty
Summary: This study reveals that the lncRNA-associated protein TOBF1 regulates the alternative splicing of transcripts necessary for maintaining stem cell identity in mouse ESCs. One of the affected genes is serine/arginine splicing factor 1 (SRSF1), whose splicing leads to global changes in splicing and expression of downstream genes involved in the maintenance of ESC pluripotency. The study shows that TOBF1 chromatin occupancy, pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and splicing converge in specific nuclear territories, collectively contributing to the maintenance of mouse ESC identity.
Article
Multidisciplinary Sciences
Tianhang Lv, Siyuan Jiang, Xiaoshan Wang, Yong Hou
Summary: In this study, we used single-cell RNA sequencing to analyze A-to-I RNA editing sites, and found that the editing frequency varied widely at the single-cell level and showed dynamic changes. We also observed a correlation between A-to-I RNA editing level and the expression of the RNA editing enzyme ADAR1. Functional analysis revealed that ADAR1-dependent A-to-I editing may regulate the expression of key genes involved in cellular response to interferon-beta and protein complex stability to promote mesenchymal-epithelial transition (MET). Furthermore, we identified a negative correlation between A-to-I RNA editing frequency and the expression of certain genes.
Article
Cell & Tissue Engineering
Martin Bergert, Sergio Lembo, Sumana Sharma, Luigi Russo, Danica Milovanovic, Kristjan H. Gretarsson, Mandy Boermel, Pierre A. Neveu, Jamie A. Hackett, Evangelia Petsalaki, Alba Diz-Munoz
Summary: Cell membrane mechanics play a crucial role in the exit from naive pluripotency of mouse embryonic stem cells, with a decrease in membrane-to-cortex attachment identified as an essential cell-intrinsic mechanism for differentiation.
Review
Biology
Boyang Liu, Han Zhao, Keliang Wu, Joerg Grosshans
Summary: Embryonic cells are able to sense temporal gradients of regulatory signals to determine the timing of cell cycle progression or remodeling, accurately linking the cell cycle with the developmental program. The mid-blastula transition serves as a paradigm for timing in early embryogenesis in various animals, with recent studies indicating multiple independent signals contribute to the timing of different processes. In Drosophila, cell cycle remodeling relies on gradual changes of signals over time, linked to Cdk1 activity through readout mechanisms involving thresholds.
Review
Neurosciences
Jiafeng Chen, Lijuan Huang, Yue Yang, Wei Xu, Qingchun Qin, Rongxing Qin, Xiaojun Liang, Xinyu Lai, Xiaoying Huang, Minshan Xie, Li Chen
Summary: Nervous system diseases pose challenges to neuroscience research due to limited access to research materials. Somatic cell reprogramming offers a novel approach to obtain neurons. This review provides an overview of the techniques involved in somatic cell reprogramming for neurological research and therapy.
Review
Cell Biology
Feng Li, Ying Ye, Xiaohua Lei, Wensheng Zhang
Summary: With the development of science and technology, mankind's exploration of outer space has increased tremendously. Settling in outer space or on other planets could help solve the Earth's resource crisis, but such settlement will first face the problem of reproduction. There are considerable differences between outer space and the Earth's environment, with the effects of gravity being one of the most significant. Studying the possible effects and underlying mechanisms of microgravity on embryonic stem cell (ESC) differentiation and embryonic development could help provide solutions to healthy living and reproduction in deep space. This article summarizes recent research progress on the effects of microgravity on ESCs and early embryonic development and proposes hypotheses regarding the potential mechanisms. In addition, we discuss the controversies and key questions in the field and indicate directions for future research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yujian Dai, Jialei Sun, Na He, Liyou An, Chunhui Hou, Fuliang Du
Summary: The regulatory function of the nucleosome remodeling and deacetylase (NuRD) complex in maintaining the pluripotency of naive mouse embryonic stem cells (mES) cultured in a protein kinase C inhibitor (PKCi) system is explored in this study. It is found that PKCi suppresses the expression of NuRD complex subunits and knockdown of MBD3, a subunit of NuRD complex, increases the pluripotency of PKCi-derived mES.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Guangsuo Xing, Zichao Liu, Luyuan Huang, Danyun Zhao, Tao Wang, Hao Yuan, Yi Wu, Linpeng Li, Qi Long, Yanshuang Zhou, Zhihong Hao, Yang Liu, Jianghuan Lu, Shiting Li, Jieying Zhu, Bo Wang, Junwei Wang, Jing Liu, Jiekai Chen, Duanqing Pei, Xingguo Liu, Keshi Chen
Summary: The study revealed that Mkk6 acts as a chromatin relaxer during reprogramming by promoting histone acetylation levels and facilitating transcription factor binding, ultimately enhancing pluripotency gene expression.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Genetics & Heredity
Yamei Li, Qiang Sun
Summary: This review summarizes the epigenetic barriers in somatic cell nuclear transfer (SCNT) embryos and discusses strategies to correct these defects and prevent abnormalities in cloned animals.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Elisa Pedone, Mario Failli, Gennaro Gambardella, Rossella De Cegli, Antonella La Regina, Diego di Bernardo, Lucia Marucci
Summary: This study found that moderate beta-catenin levels play an important role in cell differentiation, promoting the formation of EpiLC in vitro.
Article
Cell Biology
Rong Mu Xia, Dong Bo Yao, Xue Min Cai, Xiu Qin Xu
Summary: This study elucidated the regulatory role of histidine phosphatase LHPP in ESCs self-renewal through histidine dephosphorylation. LHPP negatively regulated the Wnt/β-catenin pathway by modulating histidine dephosphorylation, thereby inhibiting the self-renewal of ESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ming Sun, Bing Liao, Yu Tao, Hao Chen, Feng Xiao, Junjie Gu, Shaorong Gao, Ying Jin
JOURNAL OF CELLULAR PHYSIOLOGY
(2016)
Correction
Medicine, Research & Experimental
Lu Cui, Yuan Guan, Zepeng Qu, Jingfa Zhang, Bing Liao, Bo Ma, Jiang Qian, Dangsheng Li, Weiye Li, Guo-Tong Xu, Ying Jin
JOURNAL OF CLINICAL INVESTIGATION
(2016)
Article
Cell Biology
Bing Liao, Xiaoming Zhong, Huiming Xu, Feng Xiao, Zhuoqing Fang, Junjie Gu, Yue Chen, Yingming Zhao, Ying Jin
JOURNAL OF CELLULAR PHYSIOLOGY
(2013)
Article
Cell & Tissue Engineering
Shuang Li, Feng Xiao, Junmei Zhang, Xiaozhi Sun, Han Wang, Yanwu Zeng, Jing Hu, Fan Tang, Junjie Gu, Yingming Zhao, Ying Jin, Bing Liao
Article
Biochemistry & Molecular Biology
Jing Hu, Shuang Li, Xiaozhi Sun, Zhuoqing Fang, Lina Wang, Feng Xiao, Min Shao, Laixiang Ge, Fan Tang, Junjie Gu, Hongyao Yu, Yueshuai Guo, Xuejiang Guo, Bing Liao, Ying Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Xinyuan Liu, Zhuoqing Fang, Jing Wen, Fan Tang, Bing Liao, Naihe Jing, Dongmei Lai, Ying Jin
Article
Cell Biology
Hao Chen, Yanwu Zeng, Min Shao, Hanzhi Zhao, Zhuoqing Fang, Junjie Gu, Bing Liao, Ying Jin
Summary: This study reveals the previously unknown role of calcineurin A gamma and the NFATc3/SRPX2 axis in modulating the fate determination of human embryonic stem cells (hESCs), affecting the expression of lineage and epithelial-mesenchymal transition markers.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yujie Liu, Han Wang, Min Shao, Ying Jin, Bing Liao
Summary: Mitochondrial respiration plays a pivotal role in maintaining the stemness of mature human embryonic stem cells, and perturbations of mitochondrial respiration impair cell identity and function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Pingzhu Zhou, Yan Zhang, Isha Sethi, Lincai Ye, Michael A. Trembley, Yangpo Cao, Brynn N. Akerberg, Feng Xiao, Xiaoran Zhang, Kai Li, Blake D. Jardin, Neil Mazumdar, Qing Ma, Aibin He, Bin Zhou, William T. Pu
Summary: This study investigated the chromatin occupancy of GATA4 during heart development. The researchers found that GATA4 occupies genomic regions with lineage-specific enhancers in cardiomyocyte and endocardial lineages. They also discovered that non-pioneer transcription factors NKX2-5 and ETS1 guide the activity of GATA4, promoting its binding and opening of chromatin, and regulating endocardial development.
CIRCULATION RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Yangpo Cao, Xiaoran Zhang, Brynn N. Akerberg, Haiyun Yuan, Tomoya Sakamoto, Feng Xiao, Nathan J. VanDusen, Pingzhu Zhou, Mason E. Sweat, Yi Wang, Maksymilian Prondzynski, Jian Chen, Yan Zhang, Peizhe Wang, Daniel P. Kelly, William T. Pu
Summary: The study identified chamber-selective enhancers (CSEs) that contribute to the structural and functional differences between atrial and ventricular cardiomyocytes. Several candidate enhancers associated with cardiovascular disease were identified, and ERR alpha/gamma were found to be essential for ventricular identity. The findings provide important resources for future research on chamber-selective gene regulation.