Journal
NANOMATERIALS
Volume 7, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/nano7090248
Keywords
Gram-positive bacteria; L. casei; L. monocytogenes; Raman scattering; SERS; silver nanoparticles
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Funding
- Romanian National Authority for Scientific Research and Innovation, CNCS-UEFISCDI [PN-II-RU-TE-2014-4-0862, PN-III-P2-2.1-PED-2016-1237, 17 PED/2017]
- project Research Center and Advanced Technologies for Alternative Energies [CETATEA-623/11.03.2014]
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Raman scattering and its particular effect, surface-enhanced Raman scattering (SERS), are whole-organism fingerprinting spectroscopic techniques that gain more and more popularity in bacterial detection. In this work, two relevant Gram-positive bacteria species, Lactobacillus casei (L. casei) and Listeria monocytogenes (L. monocytogenes) were characterized based on their Raman and SERS spectral fingerprints. The SERS spectra were used to identify the biochemical structures of the bacterial cell wall. Two synthesis methods of the SERS-active nanomaterials were used and the recorded spectra were analyzed. L. casei and L. monocytogenes were successfully discriminated by applying Principal Component Analysis (PCA) to their specific spectral data.
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