Article
Chemistry, Analytical
Moon Hyeok Choi, Young Jun Seo
Summary: A novel hairpin structure-mediated diagnostic method has been developed for the colorimetric detection of miRNA, with high sensitivity (LOD = 132 aM) and selectivity, completed in just 30 minutes. The system can also detect miRNA using fluorescence with similar sensitivity, demonstrating the dual-sensing properties of the probe.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Cheng-Yu Fan, Xing Gao, Huiling Wang, Ying Xiong, Xiaoting Zou, Shi-Yu Liu
Summary: FAPa is a well-known serine protease that is highly related to tumor progression and invasion and has potential as a cancer biomarker. However, there is a lack of suitable fluorescent probes for FAPa due to their low emission windows or short Stokes shifts. In this study, a turn-on biosensor named TMN-AcGP was developed for FAPa activity sensing, which showed high sensitivity and improved optical properties for rapid and accurate detection of cancer cells and tumor tissues.
Article
Chemistry, Analytical
Tao Kong, Dong-Ying Bai, Zheng-Hui Liu, Yan-Bo Ma, Cai Zhang, Guo-Yong Wang, Shu-Hui Zhang
Summary: In this study, a colloidal gold-labeled monoclonal antibody nano-probe was synthesized for the detection of ethylenediamine tetraacetic acid (EDTA). An immunochromatographic assay based on this probe was developed, which was highly sensitive, convenient, and one-step competitive. The assay was successfully applied for the detection of EDTA in water samples, with a visual detection limit of about one ppm. It does not require any equipment or complicated sample preparation and can be completed within 10 minutes, making it suitable for on-site testing.
MICROCHEMICAL JOURNAL
(2022)
Article
Spectroscopy
Yaqian Li, Yi Zhou, Xiu Liu, Jieni Lei, Xin Qin, Guangyi Li, Zi Yang
Summary: In this paper, a near-infrared ratiometric fluorescent probe (YQ-1) was developed to detect hypochlorous acid (HClO) for comprehending its biological functions within cells. YQ-1 exhibited a fluorescence shift from red to green with a large blue shift (165 nm) in the presence of HClO, and the solution's color changed from pink to yellow. YQ-1 quickly detected HClO (within 40 s) with a low detection limit (4.47 x 10-7 mol/L) and was not affected by other interferences.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Shu-Yuan Xiao, Chang-Xiang Liu, Jing Peng, Rui Tan, Yu Peng, Ya-Wen Wang
Summary: A novel near-infrared fluorescent probe (SWJT-13) was designed and synthesized with a 3-bromopropargyne group as a recognition site for detecting Pd2+ ions. SWJT-13 can specifically detect Pd2+ ions and can be easily recognized by the naked eye under natural light. The probe has a large Stokes shift (155 nm) with a limit of detection (LOD) of 10.5 nM. The mechanism was verified using 1H NMR, MS, and Gaussian calculations. In addition, the detection of Pd2+ ions by the probe was studied in HeLa cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Dan Li, San Tu, Yi Le, Yue Zhou, Lan Yang, Yuyu Ding, Lei Huang, Li Liu
Summary: A reactive fluorescent probe SCP with high quantum yield was designed and synthesized for detection of fluoride ions. The SCP exhibited excellent performance in physiological conditions, including 62.8% quantum yield, good selectivity, and the ability to image HepG2 cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Tiantian Jiang, Weiwei Bian, Jianfei Kan, Yanyan Sun, Ning Ding, Wenjing Li, Jin Zhou
Summary: A new probe RB-CR for detecting Cr3+ was constructed based on the conjugation between rhodamine B and phenylthiourea, showing selectively, sensitively and rapidly response to Cr3+ complexation. The fluorescence imaging study suggested its potential application as a surveillance probe in living cells with low cytotoxicity, providing feasibility for exploring the Cr3+ recognition process.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Xueling Liu, Yali Wang, Guangshuai Zhou, Wenzhou Zhang
Summary: This study developed a fluorescent probe mito-ACS for imaging mitochondrial hypochlorite in living cells. The probe exhibited fast response, low detection limit, good water solubility, and selective localization to mitochondria. It showed great potential in fluorescent imaging of biological systems.
Article
Engineering, Environmental
Yunju Xiao, Weijiang Liu, Ye Zhang, Shuai Zheng, Jianfeng Liao, Huizhuang Shan, Benshun Tian, Tao Wu, Lei Zhang, Zhijie Tu, Yin Hua, Bing Gu, Xuejiao Hu
Summary: In this study, a rapid and sensitive surface-enhanced Raman scattering (SERS)-based biosensor was developed for pathogen detection. The biosensor utilized wheat germ agglutinin (WGA)-modified magnetic nanoparticles as capture elements and co-freezing aptamer/DTNB/AuNps tags as the signal source. The SERS platform showed a high analytical sensitivity, capable of detecting as low as 8 cells/mL of target bacteria. It also successfully detected DNA from difficult-to-culture or slow-growing pathogens. This sensitive and fast SERS platform has great potential for accurate and efficient identification of pathogenic bacteria from various samples.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Medicine, General & Internal
Isabel Machado, Garazi Goikoetxea, Enara Alday, Tania Jimenez, Xabier Arias-Moreno, Frank J. Hernandez, Luiza Hernandez
Summary: The identification of pathogens causing infectious diseases is currently based on laborious and time-consuming techniques. There is an urgent need for the development of novel methods that can considerably reduce detection times. Lateral flow-based systems provide fast, cheap, and easy to use alternatives for diagnosis.
Article
Agriculture, Dairy & Animal Science
Junping Wen, Hongchao Gou, Jing Liu, Hualiang Zhou, Qijie Lin, Xiaoyun Qu, Kaifeng Chen, Shaojun Wang, Haiyan Shen, Ming Liao, Jianmin Zhang
Summary: Salmonella enterica serovar Gallinarum biovars Pullorum is a bacterial pathogen causing systemic pullorum disease in the poultry industry, leading to significant losses. A specific rapid detection method targeting a single-nucleotide mutation in the rfbS gene was developed, showing high specificity and reproducibility.
Article
Multidisciplinary Sciences
Hyun-Jin Kang, Sang-Hoon Lee, Han-Shin Kim, Yong Woo Jung, Hee-Deung Park
Summary: This study proposed a method for detecting gram-negative bacteria by specifically binding polymyxin B to lipopolysaccharides. Fluorescent microscopy demonstrated that the method was sensitive enough to detect a single Escherichia coli cell and differentiate between gram-negative and gram-positive bacteria. The method could detect three cells per ml within one hour, indicating its sensitivity and rapid sensing capabilities.
Article
Biophysics
Skomantas Serapinas, Justina Gineityte, Marius Butkevicius, Rapolas Danilevicius, Marius Dagys, Dalius Ratautas
Summary: This paper presents a new bioelectronic device for the detection of nuclease activity in various fluids. The developed sensor enables rapid and sensitive determination of DNase activity, with high accuracy and reproducibility demonstrated in real human saliva samples. The technology has the potential to improve nuclease quality control processes in the pharmaceutical/biotechnology industry and provide new insights into the importance of nucleases for medical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
C. Suarez, W. R. Premasiri, H. Ingraham, A. N. Brodeur, L. D. Ziegler
Summary: A simple water extraction and transfer procedure is found to produce reproducible and highly sensitive SERS spectra of dried bloodstains on Ag nanoparticle substrates. The water/Ag methodology allows the detection and identification of diluted bloodstains on Ag substrates, while avoiding potential DNA damage. However, this water-only procedure is not effective on Au SERS substrates, due to the difference in the lysis and denaturation effects of blood cells on Ag and Au nanoparticles, respectively. The use of acetic acid is required for obtaining SERS spectra of dried bloodstains on Au substrates.
Article
Chemistry, Analytical
Xin Zhou, Hongmei Cao, Yong Zeng
Summary: In this study, a pneumatically automated microfluidic reactor device was designed to integrate analyte extraction/enrichment and DSNSA-mediated miRNA detection in a streamlined analysis workflow, achieving rapid and robust on-chip detection of miR-21 with reduced consumption of DSN enzyme. The optimized workflow demonstrated a limit-of-detection of 35 amol and has potential applications in clinical diagnosis and beyond.
Article
Surgery
Anna C. Beck, Anand Rajan, Shannon Landers, Sarah Kelley, Andrew M. Bellizzi, Geeta Lal, Sonia L. Sugg, James R. Howe, Carlos H. Chan, Ronald J. Weigel
Summary: The study showed that the tall cell variant of papillary thyroid carcinoma has increased expression of cancer stem cell markers compared to classic papillary thyroid carcinoma. A gene signature similar to the tall cell variant identified a subtype of classic papillary thyroid carcinoma with worse recurrence-free survival.
Review
Nutrition & Dietetics
Scott M. Ebert, Blake B. Rasmussen, Andrew R. Judge, Sarah M. Judge, Lars Larsson, Ronald C. Wek, Tracy G. Anthony, George R. Marcotte, Matthew J. Miller, Mark A. Yorek, Adrian Vella, Elena Volpi, Jennifer Stern, Matthew D. Strub, Zachary Ryan, John J. Talley, Christopher M. Adams
Summary: Activating transcription factor 4 (ATF4) is a multifunctional protein that regulates transcription and is involved in various cellular responses. Most of our knowledge about ATF4 comes from studies conducted in cultured cells, with limited understanding of its in vivo role. This review provides an overview of the basic biology of ATF4 and focuses on its role in mammalian skeletal muscle fibers.
JOURNAL OF NUTRITION
(2022)
Article
Oncology
Catherine G. Tran, Luis C. Borbon, Jacqueline L. Mudd, Ellen Abusada, Solmaz AghaAmiri, Sukhen C. Ghosh, Servando Hernandez Vargas, Guiying Li, Gabriella Beyer, Mary McDonough, Rachel Li, Carlos H. F. Chan, Susan A. Walsh, Thaddeus J. Wadas, Thomas O'Dorisio, M. Sue O'Dorisio, Ramaswamy Govindan, Paul F. Cliften, Ali Azhdarinia, Andrew M. Bellizzi, Ryan C. Fields, James R. Howe, Po Hien Ear
Summary: Gastroenteropancreatic neuroendocrine neoplasms (GEP NENs) are rare cancers with increasing incidence. The lack of suitable cellular and animal models for studying and testing new therapies has posed challenges for research on GEP NENs. This study successfully established two PDX models (NEC913 and NEC1452) and demonstrated their utility in drug discovery and research on GEP NENs.
Article
Psychology, Multidisciplinary
Hend M. Al-Kaylani, Bradley T. Loeffler, Sarah L. Mott, Melissa Curry, Sneha Phadke, Ellen van der Plas
Summary: This study found that young women with breast cancer do not report major health-related quality of life concerns during the first year of treatment. However, slight improvements in HRQOL were observed over a 12-month period. Anxiety/depression, marital status, reconstructive surgery, and baseline clinical staging were found to be associated with changes in HRQOL.
FRONTIERS IN PSYCHOLOGY
(2022)
Review
Obstetrics & Gynecology
Jad El Masri, Sneha Phadke
Summary: Many advancements have been made in contemporary breast cancer care, such as deescalation of therapy in low-risk populations, refining systemic therapy options, and advancing research into molecular biomarkers to achieve precision medicine goals. Supportive care guidelines and survivorship care have also been created to address the needs of an increasing number of breast cancer survivors.
CLINICAL OBSTETRICS AND GYNECOLOGY
(2022)
Article
Biochemical Research Methods
Nathan Basisty, Nicholas Shulman, Cameron Wehrfritz, Alexandra N. Marsh, Samah Shah, Jacob Rose, Scott Ebert, Matthew Miller, Dao-Fu Dai, Peter S. Rabinovitch, Christopher M. Adams, Michael J. MacCoss, Brendan MacLean, Birgit Schilling
Summary: In this study, a new computational tool, TurnoveR, was developed to accurately calculate protein turnover rates and integrated into the Skyline proteomics software. The computational pipeline of TurnoveR replicated previous study results and is expected to facilitate the broader application of protein turnover analysis in various relevant areas.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Oncology
Sneha Phadke
Summary: Neoadjuvant therapy has several potential benefits for the treatment of early-stage breast cancer, especially for patients with triple-negative or HER2+ subtypes. Recent studies have shown that adding the checkpoint inhibitor pembrolizumab to chemotherapy can significantly improve efficacy in triple-negative disease, while adjusting adjuvant treatment choices based on response to neoadjuvant therapy can help avoid certain chemotherapeutic drugs in patients with HER2+ breast cancer.
CURRENT ONCOLOGY REPORTS
(2022)
Article
Medicine, Research & Experimental
Qierra R. Brockman, Amanda Scherer, Gavin R. McGivney, Wade R. Gutierrez, Andrew P. Voigt, Alexandra L. Isaacson, Emily A. Laverty, Grace Roughton, Vickie Knepper-Adrian, Benjamin Darbro, Munir R. Tanas, Christopher S. Stipp, Rebecca D. Dodd
Summary: Loss of PRC2 has a critical role in driving metastasis in malignant peripheral nerve sheath tumors (MPNSTs), leading to increased collagen-dependent invasion, upregulation of matrix-remodeling enzymes, and elevated lung metastasis. Clinical sample analysis confirms that PRC2 loss is associated with metastatic disease, increased fibrosis, and decreased survival in MPNST patients.
Article
Medicine, Research & Experimental
Wade R. Gutierrez, Amanda Scherer, Jeffrey D. Rytlewski, Emily A. Laverty, Alexa P. Sheehan, Gavin R. McGivney, Qierra R. Brockman, Vickie Knepper-Adrian, Grace A. Roughton, Dawn E. Quelle, David J. Gordon, Varun Monga, Rebecca D. Dodd
Summary: This study explores the concept of using low-dose Decitabine to enhance the efficacy of chemotherapy. It shows that adding DAC to gemcitabine treatment improves survival and slows tumor growth in a mouse model of high-grade sarcoma. Unlike previous studies, DAC does not induce an antitumor T cell response in sarcoma and the synergy between DAC and gemcitabine is independent of the immune system.
Article
Chemistry, Multidisciplinary
Jianling Bi, Emily Witt, Vanessa. A. A. Voltarelli, Vivian. R. R. Feig, Veena Venkatachalam, Hannah Boyce, Megan McGovern, Wade. R. R. Gutierrez, Jeffrey. D. D. Rytlewski, Kate. R. R. Bowman, Ashley. C. C. Rhodes, Austin. N. N. Cook, Benjamin. N. N. Muller, Matthew. G. G. Smith, Alexis Rebecca Ramos, Heena Panchal, Rebecca. D. D. Dodd, Michael. D. D. Henry, Adam Mailloux, Giovanni Traverso, Leo. E. E. Otterbein, James. D. D. Byrne
Summary: Tumor hypoxia is a major obstacle in cancer therapy, but gas-entrapping materials (GeMs) with tunable oxygen release show promise in mitigating hypoxia and enhancing the effectiveness of radiation and chemotherapy. This study demonstrates that injection or implantation of GeMs into tumors can deliver oxygen locally and generate an antitumorigenic tumor microenvironment. Overall, the use of oxygen-releasing GeMs as an adjunctive strategy for solid tumor treatment is promising.
Article
Oncology
Jeremy Chang, Yanan Liu, Stephanie A. Saey, Kevin C. Chang, Hannah R. Shrader, Kelsey L. Steckly, Maheen Rajput, Milan Sonka, Carlos H. F. Chan
Summary: This study introduces a novel machine learning predictive model utilizing a three-dimensional convoluted neural network to improve prediction accuracy of postoperative margin and lymph node status in PDAC patients based on preoperative CT scans. Results show promising performance in predicting postoperative outcomes, with the need for further validation in larger cohorts.
FRONTIERS IN ONCOLOGY
(2022)
Article
Surgery
Mohammed O. Suraju, Anthony Snow, Apoorve Nayyar, Jeremy Chang, Scott K. Sherman, Hisakazu Hoshi, James R. Howe, Carlos H. F. Chan
Summary: This study reviewed the prevalence, risk factors, and prognosis of peritoneal metastases (PMs) following resection of pancreatic intraductal papillary mucinous neoplasms (IPMNs) using the institution's experience and published literature. The study found that PMs are rare but may be more common in patients with invasive histology. Noninvasive IPMNs can also give rise to PMs. Further research is needed to understand the pathophysiology and risk factors of PM following IPMN resection and reinforce adherence to guidelines recommending long-term surveillance.
JOURNAL OF SURGICAL RESEARCH
(2023)
Article
Geriatrics & Gerontology
Matthew J. Miller, George R. Marcotte, Nathan Basisty, Cameron Wehrfritz, Zachary C. Ryan, Matthew D. Strub, Andrew T. McKeen, Jennifer I. Stern, Karl A. Nath, Blake B. Rasmussen, Andrew R. Judge, Birgit Schilling, Scott M. Ebert, Christopher M. Adams
Summary: Aging causes a decline in skeletal muscle strength and mass, leading to functional deficits and muscle atrophy. The role of ATF4, a transcription regulatory protein, in skeletal muscle aging was investigated. Knockout mice lacking the ATF4 gene showed significant protection from age-related declines in muscle strength, exercise capacity, and muscle mass, suggesting ATF4 as a critical mediator of skeletal muscle aging and providing new insights into the degenerative process that affects older adults' health and quality of life.
Article
Medicine, Research & Experimental
George R. Marcotte, Matthew J. Miller, Hawley E. Kunz, Zachary C. Ryan, Matthew D. Strub, Patrick M. Vanderboom, Carrie J. Heppelmann, Sarah Chau, Zachary D. Von Ruff, Sean P. Kilroe, Andrew T. Mckeen, Jason M. Dierdorff, Jennifer I. Stern, Karl A. Nath, Chad E. Grueter, Vitor A. Lira, Andrew R. Judge, Blake B. Rasmussen, K. Sreekumaran Nair, Ian R. Lanza, Scott M. Ebert, Christopher M. Adams
Summary: The expression of GADD45A in skeletal muscle is associated with mitochondrial loss, atrophy, and weakness. These findings provide new insights for identifying therapeutic targets for human muscle weakness.
Article
Biochemistry & Molecular Biology
Wade R. Gutierrez, Jeffrey D. Rytlewski, Amanda Scherer, Grace A. Roughton, Nina C. Carnevale, Krisha Y. Vyas, Gavin R. McGivney, Qierra R. Brockman, Vickie Knepper-Adrian, Rebecca D. Dodd
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)