Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

(2016) Shengbiao Hu et al.   Scientific Reports

EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology

(2016) Simon J. Moore et al.   ACS Synthetic Biology

Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii as an Example

(2016) Yi Wang et al.   ACS Synthetic Biology

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

CRMAGE: CRISPR Optimized MAGE Recombineering

(2016) Carlotta Ronda et al.   Scientific Reports

Expanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii

(2016) Bastian Molitor et al.   Scientific Reports

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Construction and evaluation of a fluorescence-based live attenuated Escherichia coli delivery system for generating oral vaccine candidate

(2015) Wenxin Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184

(2015) Vandana Chakravartty et al.   PLASMID

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

(2015) Chris R. Reisch et al.   Scientific Reports

Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica

(2015) Marcos H de Moraes et al.   AMB Express

Generation of an attenuated strain oral vaccine candidate using a novel double selection platform in Escherichia coli

(2014) Wenxin Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene

(2014) Andrew W. Vail et al.   TRANSGENIC RESEARCH

R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences

(2014) Arturo Casini et al.   ACS Synthetic Biology

A New Large-DNA-Fragment Delivery System Based on Integrase Activity from an Integrative and Conjugative Element

(2013) R. Miyazaki et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Two-Plasmid Vector System for Independently Controlled Expression of Green and Red Fluorescent Fusion Proteins in Staphylococcus aureus

(2013) Anthony J. Brzoska et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system

(2013) Gu-Zhen Cui et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Generalized bacterial genome editing using mobile group II introns and Cre-lox

(2013) P. J. Enyeart et al.   Molecular Systems Biology

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Computer-Assisted Engineering of the Synthetic Pathway for Biodegradation of a Toxic Persistent Pollutant

(2013) Nagendra Prasad Kurumbang et al.   ACS Synthetic Biology

“Curing” of plasmid DNA in acetogen using microwave or applying an electric pulse improves cell growth and metabolite production as compared to the plasmid-harboring strain

(2012) Vel Berzin et al.   ARCHIVES OF MICROBIOLOGY

Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids

(2012) Fuzhong Zhang et al.   NATURE BIOTECHNOLOGY

Adaptive laboratory evolution—harnessing the power of biology for metabolic engineering

(2011) Vasiliy A Portnoy et al.   CURRENT OPINION IN BIOTECHNOLOGY

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli

(2011) G. Bokinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolution of efficient pathways for degradation of anthropogenic chemicals

(2009) Shelley D Copley   Nature Chemical Biology

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS