Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions

Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno-Free and Feeder-Free Conditions

(2017) Sacha Reichman et al.   STEM CELLS

Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human β Cell Function

(2016) H. Efsun Arda et al.   Cell Metabolism

Genome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes

(2016) Zengrong Zhu et al.   Cell Stem Cell

IDF Diabetes Atlas estimates of 2014 global health expenditures on diabetes

(2016) Joao da Rocha Fernandes et al.   DIABETES RESEARCH AND CLINICAL PRACTICE

Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling

(2016) Meike Hohwieler et al.   GUT

Generation of kidney organoids from human pluripotent stem cells

(2016) Minoru Takasato et al.   Nature Protocols

Human pancreatic beta-like cells converted from fibroblasts

(2016) Saiyong Zhu et al.   Nature Communications

Generation of stem cell-derived β-cells from patients with type 1 diabetes

(2016) Jeffrey R. Millman et al.   Nature Communications

Abnormalities of the Exocrine Pancreas in Type 1 Diabetes

(2015) Martha Campbell-Thompson et al.   Current Diabetes Reports

Islet formation in mice and men: lessons for the generation of functional insulin-producing β-cells from human pluripotent stem cells

(2015) Gopika Nair et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Human pancreas development

(2015) R. E. Jennings et al.   DEVELOPMENT

Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro

(2015) H. A. Russ et al.   EMBO JOURNAL

Nephron organoids derived from human pluripotent stem cells model kidney development and injury

(2015) Ryuji Morizane et al.   NATURE BIOTECHNOLOGY

TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors

(2015) Inês Cebola et al.   NATURE CELL BIOLOGY

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

(2015) Anca M Paşca et al.   NATURE METHODS

Ex Vivo Expansion and Differentiation of Human and Mouse Fetal Pancreatic Progenitors Are Modulated by Epidermal Growth Factor

(2015) Paola Bonfanti et al.   STEM CELLS AND DEVELOPMENT

Accelerated Maturation of Human Stem Cell-Derived Pancreatic Progenitor Cells into Insulin-Secreting Cells in Immunodeficient Rats Relative to Mice

(2015) Jennifer E. Bruin et al.   Stem Cell Reports

Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells

(2015) Kenichiro Taniguchi et al.   Stem Cell Reports

Differentiation of Human Induced Pluripotent Stem Cells into Insulin-Like Cell Clusters with miR-186 and miR-375 by using chemical transfection

(2014) Anahita Shaer et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Generation of Functional Human Pancreatic β Cells In Vitro

(2014) Felicia W. Pagliuca et al.   CELL

Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells

(2014) Alireza Rezania et al.   NATURE BIOTECHNOLOGY

The window period of NEUROGENIN3 during human gestation

(2014) Rachel J Salisbury et al.   Islets

A Massive Suspension Culture System With Metabolic Purification for Human Pluripotent Stem Cell-Derived Cardiomyocytes

(2014) Natsuko Hemmi et al.   Stem Cells Translational Medicine

FGF7 and cell density are required for final differentiation of pancreatic amylase-positive cells from human ES cells

(2013) Sakiko Takizawa-Shirasawa et al.   CELL AND TISSUE RESEARCH

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

(2013) Robert P. Fordham et al.   Cell Stem Cell

Development of the Human Pancreas From Foregut to Endocrine Commitment

(2013) R. E. Jennings et al.   DIABETES

Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence

(2013) E. Rodriguez-Seguel et al.   GENES & DEVELOPMENT

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney

(2013) M. Takasato et al.   NATURE CELL BIOLOGY

Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

(2013) T. Sato et al.   SCIENCE

Collagen IV-Modified Scaffolds Improve Islet Survival and Function and Reduce Time to Euglycemia

(2013) Woon Teck Yap et al.   TISSUE ENGINEERING PART A

Self-Renewing Endodermal Progenitor Lines Generated from Human Pluripotent Stem Cells

(2012) Xin Cheng et al.   Cell Stem Cell

Maturation of Human Embryonic Stem Cell-Derived Pancreatic Progenitors Into Functional Islets Capable of Treating Pre-existing Diabetes in Mice

(2012) A. Rezania et al.   DIABETES

A Scalable System for Production of Functional Pancreatic Progenitors from Human Embryonic Stem Cells

(2012) Thomas C. Schulz et al.   PLoS One

The Expression of the Class 1 Glucose Transporter Isoforms in Human Embryonic Stem Cells, and the Potential Use of GLUT2 as a Marker for Pancreatic Progenitor Enrichment

(2012) Hana Segev et al.   STEM CELLS AND DEVELOPMENT

Deconstructing Pancreas Developmental Biology

(2012) C. M. Benitez et al.   Cold Spring Harbor Perspectives in Biology

Stage-specific signaling through TGF  family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells

(2011) M. C. Nostro et al.   DEVELOPMENT

Pancreatic islet and progenitor cell surface markers with cell sorting potential

(2011) J. Hald et al.   DIABETOLOGIA

INS GFP/w human embryonic stem cells facilitate isolation of in vitro derived insulin-producing cells

(2011) S. J. Micallef et al.   DIABETOLOGIA

Immunohistochemical characterisation of cells co-producing insulin and glucagon in the developing human pancreas

(2011) M. J. Riedel et al.   DIABETOLOGIA

Pancreatic ductal cells in development, regeneration, and neoplasia

(2011) Maximilian Reichert et al.   JOURNAL OF CLINICAL INVESTIGATION

Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells

(2011) Olivia G Kelly et al.   NATURE BIOTECHNOLOGY

Differentiation of Mesenchymal Stem Cells Derived from Pancreatic Islets and Bone Marrow into Islet-Like Cell Phenotype

(2011) Cristina Zanini et al.   PLoS One

Functional assessment of automatically sorted pancreatic islets using large particle flow cytometry

(2011) Anja Steffen et al.   Islets

MafA and MafB Regulate Genes Critical to  -Cells in a Unique Temporal Manner

(2010) I. Artner et al.   DIABETES

Production of Functional Glucagon-Secreting  -Cells From Human Embryonic Stem Cells

(2010) A. Rezania et al.   DIABETES

Mafa expression enhances glucose-responsive insulin secretion in neonatal rat beta cells

(2010) C. Aguayo-Mazzucato et al.   DIABETOLOGIA

Partial loss of pancreas endocrine and exocrine cells of human ARX-null mutation: Consideration of pancreas differentiation

(2010) Masayuki Itoh et al.   DIFFERENTIATION

Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice

(2010) J. E. Ayala et al.   Disease Models & Mechanisms

Conversion of adult pancreatic α-cells to β-cells after extreme β-cell loss

(2010) Fabrizio Thorel et al.   NATURE

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Expansion of Cord Blood CD34+ Cells in Presence of zVADfmk and zLLYfmk Improved Their In Vitro Functionality and In Vivo Engraftment in NOD/SCID Mouse

(2010) Sangeetha V. M et al.   PLoS One

The Ectopic Expression of Pax4 in the Mouse Pancreas Converts Progenitor Cells into α and Subsequently β Cells

(2009) Patrick Collombat et al.   CELL

Inhibition of Caspase-mediated Anoikis Is Critical for Basic Fibroblast Growth Factor-sustained Culture of Human Pluripotent Stem Cells

(2009) Xiaofang Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Endocrine Cell Clustering During Human Pancreas Development

(2009) Jongmin Jeon et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Quality by design for biopharmaceuticals

(2009) Anurag S Rathore et al.   NATURE BIOTECHNOLOGY

A small molecule that directs differentiation of human ESCs into the pancreatic lineage

(2009) Shuibing Chen et al.   Nature Chemical Biology

Expansion and Differentiation of Human Embryonic Stem Cells to Endoderm Progeny in a Microcarrier Stirred-Suspension Culture

(2009) Lye T. Lock et al.   TISSUE ENGINEERING PART A

Structure–activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors

(2008) Gregory D. Cuny et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo

(2008) Evert Kroon et al.   NATURE BIOTECHNOLOGY