Article
Plant Sciences
Yao Zheng, Cecile Cabassa-Hourton, Holger Eubel, Guillaume Chevreux, Laurent Lignieres, Emilie Crilat, Hans-Peter Braun, Sandrine Lebreton, Arnould Savoure
Summary: This study reveals evidence of physical proximity and interactions among ProDH, P5CDH, and OAT in the mitochondria of Arabidopsis during dark-induced leaf senescence, suggesting the formation of a complex during P5C metabolism.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biology
Jona Merx, Rianne E. van Outersterp, Udo F. H. Engelke, Veronique Hendriks, Ron A. Wevers, Marleen C. D. G. Huigen, Huub W. A. H. Waterval, Irene M. L. W. Korver-Keularts, Jasmin Mecinovie, Floris P. J. T. Rutjes, Jos Oomens, Karlien L. M. Coene, Jonathan Martens, Thomas J. Boltje
Summary: Biomarkers of hyperprolinemia type II (HPII) were identified and characterized using metabolomics and spectroscopy methods. These biomarkers showed similar metabolic signatures as in patients with hyperprolinemia type I (HPI) and pyridoxine-dependent epilepsy, and could differentiate HPII from HPI.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roger S. Holmes
Summary: ALDH4A1 and ALDH4A2 are genes that encode mitochondrial enzymes involved in the metabolism of proline and ornithine to glutamate. The sequences and structures of ALDH4A1 are highly conserved in vertebrates and invertebrates. The human ALDH4A1 gene is more highly expressed in the liver and kidney cortex. Most vertebrates have a single ALDH4A1 gene, but there are exceptions.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Plant Sciences
Naveed Ul Mushtaq, Seerat Saleem, Inayatullah Tahir, Reiaz Ul Rehman
Summary: This study found that selenium (Se) had a better effect on the metabolism of proline in plants under salt stress. Selenium can alleviate the symptoms of salt stress and improve plant performance by promoting the accumulation of proline.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Oseeyi Daudu, Kaylen R. Meeks, Lu Zhang, Javier Seravalli, John J. Tanner, Donald F. Becker
Summary: This study investigated the Thr171Met variant of PYCR1, which is found in patients with malignant melanoma and lung adenocarcinoma. The variant significantly affects the catalytic activity of PYCR1 but does not affect thermostability. The results suggest that the impact of the T171M variant on PYCR1 function in cancer is not due to increased catalytic activity.
Article
Plant Sciences
Wei Yan, Shuting Yuan, Yazhou Zu, Zhenyi Chang, Yiqi Li, Zhufeng Chen, Gang Xie, Lei Chen, Changqing Lu, Xing Wang Deng, Chengwei Yang, Chunjue Xu, Xiaoyan Tang
Summary: Cold stress is a major limitation to rice production and distribution. However, the molecular mechanisms behind cold tolerance in rice remain unclear. This study reveals that ornithine d-aminotransferase (OsOAT) plays a role in cold tolerance during the vegetative and reproductive development of rice.
Article
Biochemistry & Molecular Biology
Sagar M. Patel, Javier Seravalli, Kyle M. Stiers, John J. Tanner, Donald F. Becker
Summary: L-Thioproline is a cyclic sulfur-containing analog of l-proline found in various organisms, and human PYCR isozymes 1 and 2 show significant dehydrogenase activity towards L-T4C. This suggests a new enzyme function for PYCRs in the metabolism of L-T4C, leading to cysteine formation.
Article
Plant Sciences
Xingwen Wang, Lan Wu, Li Xiang, Ranran Gao, Qinggang Yin, Mengyue Wang, Zhaoyu Liu, Liang Leng, Yanyan Su, Huihua Wan, Tingyu Ma, Shilin Chen, Yuhua Shi
Summary: This study compared the differences between the LAP and HAP chemotypes of Artemisia annua, the plant source of artemisinin, through comprehensive analysis of genome resequencing, metabolome, and transcriptome data. The results showed that the HAP chemotype had a higher accumulation of sesquiterpenes compared to the LAP chemotype. A gene called DBR2-Like (DBR2L) was found to be potentially involved in artemisinin biosynthesis. The analysis of DBR2L promoters revealed complicated variations among different varieties of A. annua, which may affect the expression level and yield of artemisinin.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
M. S. Nair, Y. Huang, D. A. Fidock, M. J. Towler, P. J. Weathers
Summary: The study found that A3, BUR, MED, and SAM cultivars of Artemisia annua have strong activity against five variants of SARS-CoV-2, potentially providing a cost-effective therapy to help combat the rapid global spread of these variants.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Agricultural Engineering
Shiyang Zhang, Huiting Xu, Guixian Zhong, Dawei Huang, Kerui Jiang, Jian Wu, Bo Wang
Summary: This study reports a strongly expressed LTPG in floral tissues, which is positively correlated with artemisinin biosynthetic genes. The recombinant LTPG protein displayed binding activity with fatty acids and artemisinin precursors, resulting in an increase in terpenoids and lipids. Furthermore, overexpression of LTPG repressed ethylene signaling in tobacco plants. Overall, this work illustrates the positive regulatory role of LTPG in terpenoids and lipids accumulation by exporting hydrophobic metabolites and repressing ethylene signaling.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Chunhong Cui, Jiaxin Wang, Ling Guo, Chuanyue Wu
Summary: PINCH-1 protein promotes the expression of the key enzyme P5CS involved in proline biosynthesis, affecting cell proliferation and lung adenocarcinoma progression.
Article
Biochemistry & Molecular Biology
Sagar M. Patel, Javier Seravalli, Xinwen Liang, John J. Tanner, Donald F. Becker
Summary: PYCR is an important enzyme for L-proline biosynthesis, with mutations in the PYCR2 gene linked to postnatal microcephaly and hypomyelination disorders. The disease-related variants R119C and R251C show catalytic impairment and structural defects, contributing to the pathology of these disorders.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Plant Sciences
Corentin Dourmap, Anne Marmagne, Sandrine Lebreton, Gilles Clement, Anne Guivarc'h, Arnould Savoure, Celine Masclaux-Daubresse
Summary: This study investigated the phenotypes of Arabidopsis plants with mutations in the p5cdh gene and found that these mutants had lower seed yield and produced lighter seeds, indicating defects in stem-to-seed resource allocation. Furthermore, under high nitrate supply, the defects in carbon, nitrogen, and biomass allocation to seeds in p5cdh mutants were significantly amplified. Interestingly, these defects were not observed in the prodh1 prodh2 double-mutant. This study highlights the essential role of P5CDH in the carbon and nitrogen remobilization for seed development in Arabidopsis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Magda Chalecka, Adam Kazberuk, Jerzy Palka, Arkadiusz Surazynski
Summary: The study focuses on the role of amino acid metabolism, particularly nonessential amino acids, in the regulation of various cellular processes. P5C serves as a central component in energy production and interconversion of carbons within the cell, linking multiple metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Felix Frimpong, Carel W. Windt, Dagmar van Dusschoten, Ali A. Naz, Michael Frei, Fabio Fiorani
Summary: The study found that barley genotypes carrying a wild allele at P5cs1 locus have an advantage in drought resistance at the reproductive stage by accumulating proline, maintaining photosynthetic activity, and intrinsic water use efficiency, ultimately achieving higher seed productivity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Shehla Adhami, Humaira Farooqi, Malik Z. Abdin, Ram Prasad, Asrar A. Malik
Summary: This study identified the chemical composition of Chlorophytum comosum and demonstrated its significant antiproliferative effects against lung and breast cancer cell lines, while showing no impact on normal cell lines.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Nivedita, Abdul Rawoof, Nirala Ramchiary, Malik Z. Abdin
Summary: Transcriptome analysis of P. indica under salt stress revealed 661 differentially expressed genes mainly involved in metabolic and molecular processes such as response to salt stress and oxidoreductase activity. Genes related to cell wall integrity, sterol biosynthesis, and oxidative stress were up-regulated in P. indica subjected to salt stress. These salt-responsive DEGs may play a potential role in salt stress regulation.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Vishesh Kumar, Priyanka Jain, Sureshkumar Venkadesan, Suhas Gorakh Karkute, Jyotika Bhati, Malik Zainul Abdin, Amitha Mithra Sevanthi, Dwijesh Chandra Mishra, Krishna Kumar Chaturvedi, Anil Rai, Tilak Raj Sharma, Amolkumar U. Solanke
Summary: This study analyzed the transcriptome of rice plants in response to panicle blast infection, identifying key genes involved in defense response, cell wall strengthening pathways, and potential mechanisms of panicle blast resistance. Understanding these mechanisms could aid in breeding resistant rice varieties.
Review
Genetics & Heredity
Ankit Gupta, Krishna Murari Sinha, Malik Z. Abdin, Niti Puri, Angamuthu Selvapandiyan
Summary: Currently, there is no effective vaccine for parasitic diseases, leading to a reliance on chemotherapy that is threatened by drug resistance in parasites. NDKs of pathogens have diverse functions and can be classified into two groups, with potential for developing drugs targeting group 2 NDKs. This review provides insights into the structures and functions of human NMEs and pathogen NDKs, suggesting NDKs as potential drug targets.
Article
Plant Sciences
Chunoti Changwal, Tushita Shukla, Zakir Hussain, Neera Singh, Abhijit Kar, Virendra P. Singh, M. Z. Abdin, Ajay Arora
Summary: High endogenous SA is associated with low ET evolution and high SL, while high lycopene content is correlated with high SA levels. Total antioxidants, PG, and PME decrease with fruit development, while lipid peroxidation increases. SlPAL4 and SlPAL6 genes play significant but opposite roles in regulating fruit ripening.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Bhawna Bonthala, M. Z. Abdin, Lalit Arya, Chithra D. Pandey, Vedprakash Sharma, Priya Yadav, Manjusha Verma
Summary: We developed 45,066 perfect SSR markers for bottle gourd through genome-wide analysis and utilized them for genetic diversity and population structure analysis of 96 accessions. The generated SSR markers provide a valuable tool for breeding and gene discovery in bottle gourd and other cucurbits species.
JOURNAL OF APPLIED GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Vishesh Kumar, Pankaj K. Singh, Suhas Gorakh Karkute, Mohd Tasleem, Someshwar Bhagat, M. Z. Abdin, Amitha Mithra Sevanthi, Anil Rai, Tilak Raj Sharma, Nagendra K. Singh, Amolkumar U. Solanke
Summary: In this study, researchers standardized an effective method for screening and evaluating panicle blast disease in rice. The method is simple, reliable, and suitable for large-scale screening of germplasm resources. Through gene expression analysis, the researchers evaluated the method at the phenotypic and molecular levels and successfully identified resistant wild rice genotypes and mutants.
Review
Plant Sciences
Koushik Halder, Abira Chaudhuri, Malik Z. Abdin, Manoj Majee, Asis Datta
Summary: The stress response of plants involves a complex network of signaling cascades that regulate transcriptional reprogramming to adapt to abiotic stress conditions. Changes in chromatin modification and DNA methylation have significant effects on the expression of stress-responsive genes. This review primarily focuses on chromatin restructuring under severe abiotic stresses, the crosstalk between epigenetic regulators, and the initial research on stress priming in plants.
Article
Chemistry, Multidisciplinary
Sadia Iqrar, Kudsiya Ashrafi, Shazia Khan, Monica Saifi, Nazima Nasrullah, Malik Zainul Abdin
Summary: This study identified and validated the role of miRNAs in the regulation of sulfur uptake and assimilation pathways in Brassica juncea. The findings highlight the potential implications of these molecules in enhancing sulfur content in the plant.
Article
Infectious Diseases
Mohammad Islamuddin, Abuzer Ali, Wajihul Hasan Khan, Amena Ali, Syed Kazim Hasan, Mohd Abdullah, Kentaro Kato, Malik Zainul Abdin, Shama Parveen
Summary: Chikungunya is a disease caused by an alpha virus transmitted by infected mosquitoes. Early and accurate diagnosis is crucial in managing the infection.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biotechnology & Applied Microbiology
Monica Saifi, Kudsiya Ashrafi, Nazima Nasrullah, Urvashi Bhardwaj, M. Z. Abdin
Summary: Computational analysis was used to identify miRNAs in T. cordifolia and predict their targets, revealing their potential importance in plant secondary metabolite improvement. Selected miRNAs were validated, and their expression in T. cordifolia tissues was analyzed along with their target gene expression.
Article
Chemistry, Multidisciplinary
Kudsiya Ashrafi, Sadia Iqrar, Monica Saifi, Shazia Khan, Firdaus Qamar, Syed Naved Quadri, Anuradha Mishra, Malik Zainul Abdin
Summary: This study evaluated the influence of different plant growth regulators on the density of glandular trichomes and the content of steviol glycosides in Stevia rebaudiana. The results showed that specific plant growth regulators significantly increased glandular trichome density in leaf and stem tissues of Stevia rebaudiana, leading to a notable increase in steviol glycosides and other valuable secondary metabolites. Thus, the developed protocol can be used for large-scale propagation of Stevia with improved metabolic profile.
Article
Multidisciplinary Sciences
Nazima Nasrullah, Javed Ahmad, Monica Saifi, Irum Gul Shah, Umara Nissar, Syed Naved Quadri, Kudsiya Ashrafi, Malik Zainul Abdin
Summary: Stevia contains commercially important steviol glycosides, and by over-expressing certain genes, the ratio of rebaudioside-A to stevioside can be increased. This increases the palatability and commercial viability of Stevia plants.
Article
Chemistry, Multidisciplinary
Sadia Iqrar, Kudsiya Ashra, Shazia Khan, Monica Sai, Nazima Nasrullah, Malik Zainul Abdin
Summary: This study identifies different miRNAs involved in the regulation of sulfur uptake and assimilation pathways in Brassica juncea, demonstrating their potential role through the correlation between miRNA expression pattern and target gene expression. The study also explores the implications of these miRNAs in enhancing the sulfur content of B. juncea.
Review
Plant Sciences
Koushik Halder, Abira Chaudhuri, Malik Z. Abdin, Manoj Majee, Asis Datta
Summary: RNA interference (RNAi) and CRISPR/Cas9 are important biotechnological tools in the field of agriculture. RNAi can improve disease resistance in plants, while CRISPR/Cas9 can be used to develop virus-resistant plants. In addition, the use of RNAi-induced dsRNA has great potential in various applications.
FRONTIERS IN PLANT SCIENCE
(2022)