Article
Biochemistry & Molecular Biology
Diego Carballares, Roberto Fernandez-Lafuente, Javier Rocha-Martin
Summary: This paper presents different methodologies for the immobilization of D-amino acid oxidase (DAAO). The results suggest that immobilization on glutaraldehyde support at pH 7 provides the best stability and activity for the enzyme.
PROCESS BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Rath Pichyangkura
Summary: The study prepared OPL-CLEAs for LFOS synthesis, demonstrating their advantages in catalytic conditions and stability compared to free enzymes. OPL-CLEAs efficiently synthesized LFOS with high yield and retained significant activity after multiple reuse.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Veymar G. Tacias-Pascacio, Roberto Morellon-Sterling, Daniel Castaneda-Valbuena, Angel Berenguer-Murcia, Majid Rasool Kamli, Olga Tavano, Roberto Fernandez-Lafuente
Summary: This paper reviews the immobilization of papain on different supports for the first time, with a special focus on the preparation of magnetic biocatalysts and specific problems related to the immobilization of proteases. The benefits of proper immobilization and potential artifacts are discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Chemistry, Applied
Archita Sharma, Karan Singh Thatai, Tanya Kuthiala, Gursharan Singh, Shailendra Kumar Arya
Summary: Immobilized enzymes, with their sturdy and resistant characteristics, have high catalytic activity and show potential for practical applications. The exploitation of polysaccharides as enzyme supports has gained attention for its versatility and wide application in various industries.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Review
Biotechnology & Applied Microbiology
Rafael C. Rodrigues, Angel Berenguer-Murcia, Diego Carballares, Roberto Morellon-Sterling, Roberto Fernandez-Lafuente
Summary: The use of enzyme immobilization can improve enzyme stability by generating favorable enzyme environments, preventing enzyme subunit dissociation, creating more stable enzyme conformations, or enzyme rigidification. Choosing an ideal immobilization protocol and support active groups, as well as utilizing multipoint covalent attachment, can significantly impact the effectiveness of enzyme immobilization.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Chan Hee Lee, Hye Sun Lee, Jae Won Lee, Jangyong Kim, Jin Hyung Lee, Eon Seon Jin, Ee Taek Hwang
Summary: Enzyme immobilization using inorganic materials, such as CaCO3, has been shown to improve enzyme stability and activity. In this study, in situ and crosslink-immobilized enzymes in a CaCO3 carrier were compared, with both methods preserving over 30% of initial enzyme activity after 180 days. However, differences in enzyme loading, activity, and characteristics were observed between the two methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Chemical
Victor K. Sharma, Thomas P. Binder, Alan M. Allgeier
Summary: Biocatalytic processes using immobilized enzymes have been successfully used for the efficient conversion of biomass-derived chemicals. In this study, yeast alcohol dehydrogenase (YADH) was immobilized on a functionalized polymeric resin using a covalent bonding strategy. The immobilized YADH showed high activity retention and extended longevity compared to soluble YADH, resulting in improved efficiency of ethanol-dependent furfural reduction. The study also investigated and mitigated the mass transport limitations on the immobilized-enzyme-catalyzed reaction through process design.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hung-Yueh Chen, Ching-Hsiang Lin, Chih-Yao Hou, Hui-Wen Lin, Chang-Wei Hsieh, Kuan-Chen Cheng
Summary: This study aimed to produce active ingredients siamenoside I and mogroside IV in the traditional Chinese medicine Siraitia grosvenorii using a continuous bioreactor with immobilized enzymes. Results showed that immobilized beta-glucosidase exhibited optimal activity, leading to the highest concentrations of siamenoside I and mogroside IV by controlling the flow rate.
Article
Biochemistry & Molecular Biology
Cintia Wanda Rivero, Natalia Soledad Garcia, Jesus Fernandez-Lucas, Lorena Betancor, Gustavo Pablo Romanelli, Jorge Abel Trelles
Summary: Enzyme-mediated synthesis of cladribine was successfully achieved using a novel 2'-deoxyribosyltransferase (NDT)-based biocatalyst. The immobilized derivative showed 98% retained activity, with stability in a wide range of pH and temperature, and extremely high reusability in the enzymatic production of cladribine. This study demonstrates the potential of green production of cladribine at a small scale using the immobilized biocatalyst.
Article
Chemistry, Multidisciplinary
Xuan Ji, Yao Li, Suo Wang, Xu Fei, Jing Tian, Longquan Xu, Yi Wang
Summary: This study successfully immobilized lipase by modifying magnetic hydrogel microspheres, resulting in improved stability and activity of the enzyme. The produced lipase/tannic acid magnetic hydrogel microspheres exhibited sustained high catalytic activity through consecutive cycles of reactions.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Hector Sanchez-Moran, James S. Weltz, Daniel K. Schwartz, Joel L. Kaar
Summary: "The long-term goal of biotechnology is to develop design rules for stabilizing enzymes when immobilized to materials. By investigating the stabilization of lipases immobilized on PEGMA and SBMA copolymer brush surfaces, it was found that the stability of the lipases at elevated temperatures depended on the fraction of PEGMA in the brush layer. This understanding can lead to the rational tuning of enzyme-material interfaces for reliable ruggedization of enzymes under extreme conditions."
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Roberto Morellon-Sterling, Juan M. Bolivar, Roberto Fernandez-Lafuente
Summary: The immobilization of ficin on vinyl sulfone agarose caused its almost complete inactivation, possibly due to the modification of catalytic cysteine. By switching off ficin with dipyridyl-disulfide during immobilization and switching it on with 1 M beta-mercaptoethanol, the activity of the immobilized ficin could be significantly increased.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Infectious Diseases
Marik M. Mueller, Ruslan Nedielkov, Katja M. Arndt
Summary: This study focuses on the contamination and antimicrobial resistance caused by the antibiotic florfenicol in animal farming and aquaculture. The researchers developed a novel enzymatic strategy for florfenicol inactivation and optimized a hydrolase for this purpose. The hydrolase remained active in different media and various environmentally-friendly application strategies were developed. The findings have important implications for the treatment of waste milk and aquacultural wastewater.
Article
Food Science & Technology
Pamela M. da Silva, Eli Emanuel Esparza-Flores, Anike H. Virgili, Eliana W. de Menezes, Roberto Fernandez-Lafuente, Lucas Dal Magro, Rafael C. Rodrigues
Summary: This study investigated the impact of different support matrices and active groups on the nutritional properties of orange juice after clarification. The results showed that immobilized enzyme on chitosan activated using genipin exhibited the highest catalytic activity and effectively reduced the turbidity of orange juice. The enzymatic treatment also maintained the natural sugar content, total soluble solids, and pH of the juice but caused color changes.
Review
Biotechnology & Applied Microbiology
Sara Arana-Pena, Diego Carballares, Roberto Morellon-Sterlling, Angel Berenguer-Murcia, Andres R. Alcantara, Rafael C. Rodrigues, Roberto Fernandez-Lafuente
Summary: Enzyme co-immobilization offers kinetic advantages but also drawbacks, such as the need to discard the entire biocatalyst if one enzyme becomes inactivated. The co-immobilization of cofactors and enzymes is of particular interest for the construction of artificial cells and complex one-pot transformations.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Applied
Tiago Lima de Albuquerque, Sandy Danielle Luncindo Gomes, Jose Edvan Marques, Ivanildo Jose da Silva, Maria Valderez Ponte Rocha
Article
Biochemistry & Molecular Biology
Daniela B. Hirata, Tiago L. Albuquerque, Nazzoly Rueda, Jose J. Virgen-Ortiz, Veymar G. Tacias-Pascacio, Roberto Fernandez-Lafuente
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2016)
Article
Biochemistry & Molecular Biology
Nazzoly Ruedaa, Cleiton S. dos Santos, Maria Daniela Rodriguez, Tiago L. Albuquerque, Oveimar Barbosa, Rodrigo Torres, Claudia Ortiz, Roberto Fernandez-Lafuente
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2016)
Article
Biochemistry & Molecular Biology
Nazzoly Rueda, Tiago L. Albuquerque, Rocio Bartolome-Cabrero, Laura Fernandez-Lopez, Rodrigo Torres, Claudia Ortiz, Jose C. S. dos Santos, Oveimar Barbosa, Roberto Fernandez-Lafuente
Article
Biochemistry & Molecular Biology
Laura Fernandez-Lopez, Nazzoly Rueda, Rocio Bartolome-Cabrero, Maria D. Rodriguez, Tiago L. Albuquerque, Jose C. S. dos Santos, Oveimar Barbosa, Roberto Fernandez-Lafuente
PROCESS BIOCHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Tiago L. de Albuquerque, Sara Peirce, Nazzoly Rueda, Antonio Marzocchella, Luciana R. B. Goncalves, Maria Valderez Ponte Rocha, Roberto Fernandez-Lafuente
PROCESS BIOCHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Tiago L. de Albuquerque, Nazzoly Rueda, Jose C. S. dos Santos, Oveimar Barbosa, Claudia Ortiz, Baris Binay, Ece Ozdemir, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
PROCESS BIOCHEMISTRY
(2016)
Article
Chemistry, Multidisciplinary
Daniela B. Hirata, Tiago L. Albuquerque, Nazzoly Rueda, Jose M. Sanchez-Montero, Eduardo Garcia-Verdugo, Raul Porcar, Roberto Fernandez-Lafuente
Article
Engineering, Environmental
Maria Valderez Ponte Rocha, Tigressa Helena Soares Rodrigues, Tiago Lima de Albuquerque, Luciana Rocha B. Goncalves, Gorete Ribeiro de Macedo
CHEMICAL ENGINEERING JOURNAL
(2014)
Review
Biochemistry & Molecular Biology
Tiago Lima de Albuquerque, Ivanildo Jose da Silva, Gorete Ribeiro de Macedo, Maria Valderez Ponte Rocha
PROCESS BIOCHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Tiago Lima de Albuquerque, Sandy Danielle Lucindo Gomes, Alan Portal D'Almeida, Roberto Fernandez-Lafuente, Luciana Rocha Barros Goncalves, Maria Valderez Ponte Rocha
PROCESS BIOCHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Maria de Fatima Matos de Freitas, Lucas C. Hortencio, Tiago Lima de Albuquerque, Maria Valderez Ponte Rocha, Luciana Rocha Barros Goncalves
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2020)
Article
Biochemistry & Molecular Biology
Ariane G. Santos, Tiago L. de Albuquerque, Bernardo D. Ribeiro, Maria Alice Z. Coelho
Summary: This article discusses the wide application of biotechnology and bioengineering techniques in production, particularly in the production of biofuels, chemicals, pharmaceuticals, and food additives. It also introduces new production processes like in situ product recovery or extractive fermentation, as well as main bioproducts and technological concepts mentioned in recent literature.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2021)
Article
Engineering, Chemical
Tiago Lima de Albuquerque, Sandy Danielle Lucindo Gomes, Ivanildo Jose da Silva Junior, Luciana Rocha Barros Goncalves, Maria Valderez Ponte Rocha
Summary: This work evaluates xylitol production by three yeasts from different carbon sources and evaluates the potential of xylitol production from hemicellulose hydrolysate of cashew bagasse.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)