How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

Monitoring of malolactic fermentation in wine using an electrochemical bienzymatic biosensor for l-lactate with long term stability

(2016) Pablo Giménez-Gómez et al.   ANALYTICA CHIMICA ACTA

Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease

(2016) Yasemin Ispirli Doğaç et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Protein engineering of microbial cholesterol oxidases: a molecular approach toward development of new enzymes with new properties

(2016) Zahra Moradpour et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improvement of the stability and activity of immobilized glucose oxidase on modified iron oxide magnetic nanoparticles

(2016) Mahboube Abbasi et al.   APPLIED SURFACE SCIENCE

Stabilization of lipase from Thermomyces lanuginosus by crosslinking in PEGylated polyurethane particles by polymerization: Application on fish oil ethanolysis

(2016) Eliane Pereira Cipolatti et al.   BIOCHEMICAL ENGINEERING JOURNAL

Effect of Mutation and Substrate Binding on the Stability of Cytochrome P450BM3 Variants

(2016) Inacrist Geronimo et al.   BIOCHEMISTRY

A Central Cysteine Residue Is Essential for the Thermal Stability and Function of SUMO-1 Protein and SUMO-1 Peptide–Protein Conjugates

(2016) Hervé Drobecq et al.   BIOCONJUGATE CHEMISTRY

Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability

(2016) Sarah Moraïs et al.   Biotechnology for Biofuels

Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

(2016) Wei Xia et al.   Biotechnology for Biofuels

Enhanced Heat Stability of α-Chymotrypsin through Single-Enzyme Confinement in Attoliter Liposomes

(2016) Makoto Yoshimoto et al.   CHEMBIOCHEM

Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

(2016) Nazzoly Rueda et al.   CHEMICAL RECORD

Enzyme immobilization on a nanoadsorbent for improved stability against heavy metal poisoning

(2016) R.P. Pogorilyi et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Assessment of the fouling mechanisms of an ultrafiltration membrane bioreactor during synthesis of galacto-oligosaccharides: Effect of the operational variables

(2016) Andrés Córdova et al.   DESALINATION

Effect of changing the nanoscale environment on activity and stability of nitrate reductase

(2016) Veena Sachdeva et al.   ENZYME AND MICROBIAL TECHNOLOGY

Immobilization of papaya laccase in chitosan led to improved multipronged stability and dye discoloration

(2016) Nivedita Jaiswal et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability

(2016) Enping Lai et al.   JOURNAL OF APPLIED POLYMER SCIENCE

Thermostability of Enzymes from Molecular Dynamics Simulations

(2016) Tim Zeiske et al.   Journal of Chemical Theory and Computation

Urease immobilized polymer hydrogel: Long-term stability and enhancement of enzymatic activity

(2016) S.N. Raju Kutcherlapati et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

Highly stable enzymatic membrane for fast treatment of antibiotic-polluted water

(2016) Zhengyang Zhao et al.   JOURNAL OF MEMBRANE SCIENCE

Covalent immobilization of glucose oxidase on mesocellular silica foams: Characterization and stability towards temperature and organic solvents

(2016) N. Balistreri et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Recyclable solid-phase biocatalyst with improved stability by sol–gel entrapment of β-d-galactosidase

(2016) Emese Biró et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Contribution of main chain and side chain atoms and their locations to the stability of thermophilic proteins

(2016) Dharma Rao Tompa et al.   JOURNAL OF MOLECULAR GRAPHICS & MODELLING

Encapsulation of a Nerve Agent Detoxifying Enzyme by a Mesoporous Zirconium Metal–Organic Framework Engenders Thermal and Long-Term Stability

(2016) Peng Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection

(2016) Hao Liang et al.   Nanoscale

Enhancing protein stability with extended disulfide bonds

(2016) Tao Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ion exchange of β-galactosidase: The effect of the immobilization pH on enzyme stability

(2016) Tiago L. de Albuquerque et al.   PROCESS BIOCHEMISTRY

Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment

(2016) Tiago L.de Albuquerque et al.   PROCESS BIOCHEMISTRY

Bioinspired stability improvement of layer-by-layer microcapsules using a bioadhesive for enzyme encapsulation

(2016) Lei Zhang et al.   REACTIVE & FUNCTIONAL POLYMERS

Covalent biofunctionalization of chitosan nanofibers with trypsin for high enzyme stability

(2016) Jana Srbová et al.   REACTIVE & FUNCTIONAL POLYMERS

Improved activity and thermo-stability of the horse radish peroxidase with graphene quantum dots and its application in fluorometric detection of hydrogen peroxide

(2016) Zhou Xiaoyan et al.   SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion

(2016) Zhao Zhao et al.   Nature Communications

Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis

(2016) Prakash Chandra Rathi et al.   PLoS Computational Biology

Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001

(2016) Neha Srivastava et al.   Frontiers in Microbiology

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

(2016) Shihui Wang et al.   Catalysts

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

(2016) Johannes Then et al.   FEBS Open Bio

Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis

(2016) Boyang Guo et al.   PLoS One

Environmental Factors Modulating the Stability and Enzymatic Activity of the Petrotoga mobilis Esterase (PmEst)

(2016) Jose L. S. Lopes et al.   PLoS One

Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution

(2015) Mikito YASUZAWA et al.   ANALYTICAL SCIENCES

Enzyme and Mediator-coadsorbed Carbon Felt Electrode for Electrochemical Detection of Glucose Covered with Polymer Layers Based on Layer-by-Layer Technique

(2015) Soichi YABUKI et al.   ANALYTICAL SCIENCES

Enhanced Thermostability of Lipoxygenase from Anabaena sp. PCC 7120 by Site-Directed Mutagenesis Based on Computer-Aided Rational Design

(2015) Hanwen Diao et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization

(2015) Mailin Misson et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

(2015) Nur Royhaila Mohamad et al.   BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

Thermostable α-amylase immobilization: Enhanced stability and performance for starch biocatalysis

(2015) Gudi Satheesh Kumar et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents

(2015) Mohammad Pazhang et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery

(2015) Hiroaki Sakamoto et al.   BIOTECHNOLOGY LETTERS

Nanodevices for the immobilization of therapeutic enzymes

(2015) Valeria E. Bosio et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine

(2015) Silvia G. Padilla-Martínez et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Influences of operating conditions on continuous lactulose synthesis in an enzymatic membrane reactor system: A basis prior to long-term operation

(2015) Azis Boing Sitanggang et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization

(2015) Jeffrey Chun Yu Wu et al.   JOURNAL OF BIOTECHNOLOGY

Magnetic mesoporous enzyme-silica composites with high activity and enhanced stability

(2015) Jiandong Cui et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Laccase immobilization over multi-walled carbon nanotubes: Kinetic, thermodynamic and stability studies

(2015) Ana P.M. Tavares et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

Partial purification and characterisation of two actinomycete tyrosinases and their application in cross-linking reactions

(2015) Marilize Le Roes-Hill et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachment

(2015) Jose C.S. dos Santos et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method

(2015) Kyu Hwan Hyun et al.   JOURNAL OF POWER SOURCES

Molecular-Level Insights into Orientation-Dependent Changes in the Thermal Stability of Enzymes Covalently Immobilized on Surfaces

(2015) Tadeusz L. Ogorzalek et al.   LANGMUIR

Stability and enzyme activity of lysozyme in the presence of Fe3O4 nanoparticles

(2015) B. Shareghi et al.   MONATSHEFTE FUR CHEMIE

Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

(2014) Tetsuji Itoh et al.   ANALYST

Supporting Materials That Improve the Stability of Enzyme Membranes

(2014) Soichi YABUKI   ANALYTICAL SCIENCES

Toward cell-free biofuel production: Stable immobilization of oligomeric enzymes

(2014) J. Grimaldi et al.   BIOTECHNOLOGY PROGRESS

Directed Evolution of a Formate Dehydrogenase for Increased Tolerance to Ionic Liquids Reveals a New Site for Increasing the Stability

(2014) Julie L. L. Carter et al.   CHEMBIOCHEM

Chemical modification of chloroperoxidase for enhanced stability and activity

(2014) Milja Pešić et al.   PROCESS BIOCHEMISTRY

Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane

(2014) Soichi Yabuki et al.   Materials

A repeatedly refuelable mediated biofuel cell based on a hierarchical porous carbon electrode

(2014) Shuji Fujita et al.   Scientific Reports

Layer-by-layer Nanoarchitectonics: Invention, Innovation, and Evolution

(2013) Katsuhiko Ariga et al.   CHEMISTRY LETTERS

New trends in enzyme immobilization at nanostructured interfaces for efficient electrocatalysis in biofuel cells

(2013) A. de Poulpiquet et al.   ELECTROCHIMICA ACTA

Dipeptide synthesis in near-anhydrous organic media: Long-term stability and reusability of immobilized Alcalase

(2013) P. Vossenberg et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Bio-based Films from Linter Cellulose and Its Acetates: Formation and Properties

(2013) Daniella Morgado et al.   Materials

Thermal and rheological properties of cellulose-graft-polyacrylamide synthesized by in situ graft copolymerization

(2013) Yue Zhang et al.   RSC Advances

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

(2012) Katsuhiko SATO et al.   ANALYTICAL SCIENCES

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

(2012) Soichi YABUKI et al.   ANALYTICAL SCIENCES

Shear and Extensional Rheology of Cellulose/Ionic Liquid Solutions

(2012) Simon J. Haward et al.   BIOMACROMOLECULES

An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity

(2012) Karel De Winter et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Recent advances of ionic liquids in separation science and mass spectrometry

(2012) Manishkumar D. Joshi et al.   RSC Advances

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

(2011) Soichi YABUKI   ANALYTICAL SCIENCES

Stability of lipase immobilized on O-pentynyl dextran

(2011) Muhammad Nazir Tahir et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Ionic Liquid-Inspired Cations Covalently Bound to Formate Dehydrogenase Improve its Stability and Activity in Ionic Liquids

(2011) Mourad Bekhouche et al.   ChemCatChem

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

(2011) Don A. Cowan et al.   ENZYME AND MICROBIAL TECHNOLOGY

Investigation of the structure and proteolytic activity of papain in aqueous miscible organic media

(2011) Carlos R. Llerena-Suster et al.   PROCESS BIOCHEMISTRY

Effects of water miscible organic solvents on the activity and conformation of the baeyer-villiger monooxygenases from Thermobifida fusca and Acinetobacter calcoaceticus: A comparative study

(2010) Francesco Secundo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enhanced stability of enzyme organophosphate hydrolase interfaced on the carbon nanotubes

(2010) Valber A. Pedrosa et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Electrochemical reaction of fructose dehydrogenase on carbon cryogel electrodes with controlled pore sizes

(2010) Seiya Tsujimura et al.   ELECTROCHEMISTRY COMMUNICATIONS

Amperometric assessment of glucose electrode behaviour in mixed solvents and determination of glucose in dairy products

(2010) Antonela Ninčević Grassino et al.   FOOD CHEMISTRY

Dependence of catalytic activity and long-term stability of enzyme hydrogel films on curing time

(2009) Joshua Lehr et al.   BIOELECTROCHEMISTRY

Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes

(2009) Juan M. Bolivar et al.   BIOMACROMOLECULES

Permeation regulation of charged species by the component change of polyion complex membranes

(2008) Soichi Yabuki et al.   ANALYTICAL BIOCHEMISTRY

Long-term activity of covalent grafted biocatalysts during intermittent use of a glucose/O2 biofuel cell

(2008) G. Merle et al.   ELECTROCHIMICA ACTA

Hydrogen peroxide biosensor based on a polyion complex membrane containing peroxidase and toluidine blue, and its application to the fabrication of a glucose sensor

(2008) Soichi Yabuki et al.   MICROCHIMICA ACTA

Control of internal (2D and 3D hexagonal) mesostructure and particle morphology of spherical mesoporous silica particles using the emulsion and solvent evaporation (ESE) method

(2008) Malin H. Sörensen et al.   MICROPOROUS AND MESOPOROUS MATERIALS

Surface modification of thin polyion complex film for surface plasmon resonance immunosensor

(2007) R KURITA et al.   SENSORS AND ACTUATORS B-CHEMICAL