Article
Fisheries
Nattanan Srisakultiew, Suksan Kumkhong, Lucie Marandel, Elisabeth Plagnes-Juan, Stephane Panserat, Surintorn Boonanuntanasarn
Summary: The objective of this study was to investigate the effects of different durations of early carbohydrate feeding stimulus on the growth performance and carbohydrate metabolism of Nile tilapia later in life. The results showed that early high carbohydrate/low protein stimulus improved the growth performance of juveniles and enhanced their utilization of dietary carbohydrates. Metabolism modifications linked to early stimulus were observed irrespective of the duration of early stimulus.
Article
Biochemistry & Molecular Biology
Yunqiu Wang, Mengqi Jia, Chuanjie Liang, Nan Sheng, Xiaodan Wang, Fang Wang, Yanhai Luo, Jin Jiang, Liangyu Cai, Huanmin Niu, Deyu Zhu, Effat Un Nesa, Charles Y. F. Young, Huiqing Yuan
Summary: AGR2 plays a crucial role in fatty acid uptake and activation, interacting with FABP1 to affect lipid accumulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Genetics & Heredity
Tiange Feng, Weizhen Zhang, Ziru Li
Summary: This review summarizes the cargo substances detected in intestinal extracellular vesicles and connects these molecules with the fine-tuning regulation of glucose and lipid metabolism in liver, muscle, pancreas, and adipose tissue.
Article
Fisheries
Wen-Hao Zhou, Chen-Chen Wu, Samwel M. Limbu, Rui-Xin Li, Li-Qiao Chen, Fang Qiao, Yuan Luo, Mei-Ling Zhang, Tao Han, Zhen-Yu Du
Summary: This study investigated the effects of different dietary carbohydrate complexities on fish metabolism and liver health in Nile tilapia. The results showed that Nile tilapia utilizes polysaccharides and disaccharides better than monosaccharides, and feeding on monosaccharides causes insulin resistance and glucose metabolism disorders.
Article
Fisheries
Rui-Xin Li, Ling-Yun Chen, Bing Yao, Samad Rahimnejad, Jiong Ren, Yuan Luo, Fang Qiao, Mei-Ling Zhang, Zhen-Yu Du
Summary: Trehalose supplementation can reduce liver cholesterol accumulation and alleviate liver damage caused by a high-cholesterol diet in Nile tilapia, but these effects are not related to autophagy activities.
Article
Fisheries
Rui-Xin Li, Ling-Yun Chen, Bing Yao, Samad Rahimnejad, Jiong Ren, Yuan Luo, Fang Qiao, Mei-Ling Zhang, Zhen-Yu Du
Summary: Trehalose has been found to reduce liver and serum cholesterol accumulation in Nile tilapia fed a high-cholesterol diet, but this effect is not related to autophagy activities.
Article
Fisheries
Huiwen Sun, Dongyan Guan, Jiting Wang, Zhen Wang, Yang Li, Haojun Han, Xiang Li, Tingting Fang
Summary: The addition of glimepiride at an optimal dosage can improve insulin secretion and liver/muscle glycogen synthesis in GIFT tilapia, without significantly impacting growth indexes.
AQUACULTURE REPORTS
(2021)
Review
Pharmacology & Pharmacy
Hewen Jiang, Dijie Li, Ying Han, Nanxi Li, Xiaohui Tao, Jin Liu, Zongkang Zhang, Yuanyuan Yu, Luyao Wang, Sifan Yu, Ning Zhang, Huan Xiao, Xin Yang, Yihao Zhang, Ge Zhang, Bao-Ting Zhang
Summary: Lipid and glucose metabolism are crucial for human activities and their dysregulation can lead to diabetes and obesity. Recent studies have shown that the bone, acting as an endocrine organ, plays a role in systemic metabolism through bone-derived molecules. Sclerostin, a protein produced by osteocytes, not only inhibits bone formation but also affects lipid and glucose metabolism. This paper provides a comprehensive review of the effects of sclerostin on lipid and glucose metabolism, discusses the progress in sclerostin research, and explores its potential manipulation for obesity and diabetes treatment.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jing Gu, Mengya Geng, Mengxiang Qi, Lizhuo Wang, Yao Zhang, Jialin Gao
Summary: Lysosomal membrane proteins play crucial roles in glucose and lipid metabolism, interacting with transport proteins like GLUT8, NPC1, and NPC2. Knockout animals of CLC-5 and SIDT2 show metabolic disorders, and proteins like V-ATPase and LAMP-2 interact with those related to glucose and lipid metabolism, highlighting the importance of LMPs in glycolipid metabolism.
Article
Fisheries
Lijuan Yu, Juan Tian, Chenxi Zhang, Zhiyong Xue, Xing Lu, Hua Wen, Ming Jiang, Fan Wu
Summary: This study investigated the effects of different levels of acetylferulic paeonol ester (APE) on the growth performance, body composition, serum biochemical indicators, and lipid and glucose metabolism of Nile tilapia. The results showed that APE supplementation improved weight gain and decreased liver lipid content. It also affected serum biochemical parameters related to lipid and glucose metabolism. Transcriptome analysis revealed changes in gene expression related to fatty acid oxidation and degradation. APE influenced the hepatic glucose and lipid metabolism of tilapia at both enzymatic and mRNA levels.
Article
Biochemistry & Molecular Biology
Mobina Alemi, Angela Oliveira, Sofia C. Tavares, Jose Ricardo Vieira, Marco G. Alves, Pedro F. Oliveira, Isabel Cardoso
Summary: TTR haploinsufficiency (TTR+/-) leads to elevated glucose levels in plasma and primary hepatocyte culture media, as well as reduced expression of glucose transporters GLUT1, GLUT3, and GLUT4. TTR also increases mitochondrial density in the liver and protects against mitochondrial oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Giusi Ianiro, Antonella Niro, Luigi Rosa, Piera Valenti, Giovanni Musci, Antimo Cutone
Summary: Pathological conditions such as obesity, diabetes, hypertension, heart disease, and cancer are associated with abnormal metabolic states, commonly referred to as the metabolic syndrome. Lactoferrin (Lf), a nutraceutical protein, can play a dual role in human metabolism, either boosting or resetting it under physiological and pathological conditions. Lf consumption is safe and has several benefits for human health, including the control of glucose and lipid metabolism, reduction of inflammation, and regulation of iron absorption and balance. It can be recommended as a promising natural adjuvant for the treatment of metabolic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Gastroenterology & Hepatology
Renate A. A. A. Ruigrok, Rinse K. Weersma, Arnau Vich Vila
Summary: The review highlights the importance of studying the microbial communities in the small intestine, which has been largely overlooked compared to the fecal microbiota. It provides an overview of methods for studying the composition and dynamics of the small intestinal microbiota and discusses its role in maintaining physiological functions and the development of diseases. Understanding and characterizing the small intestinal microbiota has the potential to advance gut microbiome research and the development of diagnostic and therapeutic approaches for diseases.
Article
Environmental Sciences
Ruijia Zhang, Wenhua Lu, Lanyin Tu, Yingshi Lin, Jin Sun, Baowei Chen, Tiangang Luan
Summary: This study used metabolic flux analysis to investigate the interconnections of glucose, glutamine, and fatty acids in PFOA-treated human colorectal cancer cells. The results showed that PFOA treatment led to metabolic remodeling in intestinal cells, potentially related to the loss of glucose in biomass synthesis and energy metabolism.
ENVIRONMENTAL POLLUTION
(2023)
Review
Cell Biology
Nadya M. Morrow, Antonio A. Hanson, Erin E. Mulvihill
Summary: Enteroendocrine cells play a crucial role in regulating nutrient intake and disposal through the production and secretion of peptides. Understanding the identity of cells expressing Glp1r, Glp2r, and Gipr in different models can provide insights into dysregulation seen in obesity and diabetes. Further research on G-protein coupled receptor circuits in the intestine and their alterations with high-fat diet feeding is also important in understanding metabolic diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Marine & Freshwater Biology
Minghui Li, Shengfei Dai, Xingyong Liu, Hesheng Xiao, Deshou Wang
Summary: Reverse genetics approaches, especially CRISPR/Cas9, are essential for understanding complex biological processes and genetic engineering. Nile tilapia, with its XX/XY sex determination system, serves as a valuable model for studying fish sex determination and reproduction. The detailed protocol provided in this study will facilitate the application of CRISPR/Cas9 in tilapia research and aquaculture studies.
Article
Biochemistry & Molecular Biology
Wenjing Tao, Luohao Xu, Lin Zhao, Zexian Zhu, Xin Wu, Qianwen Min, Deshou Wang, Qi Zhou
Summary: Researchers have reported the newly produced genomes of Nile tilapia and blue tilapia, providing detailed insights into their sex chromosome turnover mechanisms and sex-determining pathways, which are crucial for the development of genetic sex control in aquaculture. The study suggests that unequal distribution of repeat content may impact the local recombination rate, making some chromosomes more likely to become sex chromosomes.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Biochemistry & Molecular Biology
Feilong Wang, Zuliang Qin, Zhiqiang Li, Shuangyi Yang, Tian Gao, Lina Sun, Deshou Wang
Summary: In this study, the precise expression patterns of dnmt3aa and dnmt3ab in tilapia gonads were elucidated. Mutation of dnmt3aa and dnmt3ab were achieved, showing that dnmt3aa(-/-) mutants exhibited lower GSI, increased apoptosis, and reduced sperm quality, while dnmt3ab(-/-) mutants displayed normal gonadal development. Results suggest that dnmt3aa, but not dnmt3ab, plays important roles in maintaining gametogenesis in teleosts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Evolutionary Biology
Chenxu Wang, Baoyue Lu, Tao Li, Guangyuan Liang, Mengmeng Xu, Xingyong Liu, Wenjing Tao, Linyan Zhou, Thomas D. Kocher, Deshou Wang
Summary: The diverse color patterns of cichlid fishes are important in mate choice and speciation. Different genes control body and eye pigmentation in Nile tilapia. Several genes, including mitf, kita/kitlga, and pmel, are significant for color patterning in tilapia, making it an excellent model system for studying vertebrate color patterning.
JOURNAL OF HEREDITY
(2021)
Article
Biochemistry & Molecular Biology
Zhisheng Ma, Jing Yang, Qingqing Zhang, Chunmei Xu, Jing Wei, Lina Sun, Deshou Wang, Wenjing Tao
Summary: miR-133b, a member of the miR-133 family, plays a crucial role in tilapia oogenesis by regulating the expression of tagln2 gene and impacting estrogen synthesis. The study validates the role of miR-133b in fish reproduction through experiments in mice, laying the foundation for further exploration of the regulatory network in fish oogenesis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Lanying Yang, Xuefeng Zhang, Shujun Liu, Chenhua Zhao, Yiyang Miao, Li Jin, Deshou Wang, Linyan Zhou
Summary: In teleost fish, the gene Cyp17a1 plays a crucial role in steroidogenesis, sex determination, and fertility. Mutations in the cyp17a1 gene in Nile tilapia led to female-to-male sex reversal and deficiencies in hormone production, impacting both the sex determination and reproductive processes in fish. Overall, Cyp17a1 is found to be essential for E2 production in female sex determination and differentiation, as well as for T and 11-KT production in promoting spermatogenesis and fertility in male fish.
Review
Endocrinology & Metabolism
Linyan Zhou, Minghui Li, Deshou Wang
Summary: The involvement of sex steroids in sex determination and differentiation in non-mammalian vertebrates, especially in fish, is relatively conserved. Advances in genome sequencing and editing have led to significant progress in understanding steroidogenic pathways and hormonal regulation of sex determination in fish. Gene editing studies challenge traditional views on the roles of estrogen and androgen in ovarian and testicular development, and highlight the critical role of estrogen in female sex determination.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu Li, You Wu, Chenhua Zhao, Yiyang Miao, Jing Cai, Lingyun Song, Jing Wei, Tapas Chakraborty, Limin Wu, Deshou Wang, Linyan Zhou
Summary: The StAR2 gene plays a critical role in testicular development, spermatogenesis, and spermiation in tilapia by regulating androgen production. However, it may not be essential and could be compensated for in the absence of StAR2.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Xingyong Liu, Shengfei Dai, Jiahong Wu, Xueyan Wei, Xin Zhou, Mimi Chen, Dejie Tan, Deyong Pu, Minghui Li, Deshou Wang
Summary: The study analyzed the expression and functions of amh duplicates in Nile tilapia. It was found that amhy is expressed earlier and at a higher level during sex determination, and it determines sex by repressing the transcription of cyp19a1a. Mutations in amhy lead to sex reversal and altered germ cell proliferation.
Article
Evolutionary Biology
Chenxu Wang, Jia Xu, Thomas D. Kocher, Minghui Li, Deshou Wang
Summary: In this study, researchers identified two pmel genes in Nile tilapia and demonstrated their importance in pigmentation. Homozygous mutation of pmela resulted in a yellowish body color with weak vertical bars, while mutation of pmelb had a milder effect. Double mutation of both genes led to a decrease in melanophores and an increase in xanthophores, resulting in a golden body color. The findings provide new strategies for breeding golden tilapia and offer a new model for studying pmel function in vertebrates.
JOURNAL OF HEREDITY
(2022)
Article
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
Summary: Teleosts are important models for studying sex chromosomes and sex-determining genes. In this study, a high-contiguity chromosome-level genome assembly on a YY southern catfish was generated using Nanopore and Hi-C technologies. The study identified a candidate sex-determining gene in the sex-determining region and confirmed the role of amhr2y in male sex determination.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Chunmei Xu, Miao Yu, Qingqing Zhang, Zhisheng Ma, Kang Du, Huiqin You, Jing Wei, Deshou Wang, Wenjing Tao
Summary: In this study, the BRD gene family was identified and characterized in Nile tilapia, revealing sexually dimorphic expression patterns and their critical role in spermatogenesis. The findings provide insights into the understanding of sex differentiation and gonadal development in teleosts.
Correction
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xingyong Liu, Li Zhou, Wenbo Li, Jiahong Wu, Deshou Wang
Summary: In this study, it was found that GATA2 plays a critical role in lens morphogenesis in Nile tilapia. Mutations in the gata2a gene led to degeneration and apoptosis of lens fiber cells, causing progressive microphthalmia and blindness in adulthood. Transcriptome analysis revealed significant changes in the expression levels of genes encoding crystallin and genes involved in visual perception and metal ion binding after gata2a mutation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei
Summary: In this study, an integrated CRISPR/Cas9 system was established for gene knock-out and knock-in at a specific site in medaka. The system is simple, cost-effective, and efficient. The results showed that pCas9-mU6sgRNA driven by endogenous U6 promoter achieved high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. This study provides insights into other fish genome editing and promotes gene functional analysis.