Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

(2016) Tomomi Aida et al.   BMC GENOMICS

In vivoandin vitrodisease modeling with CRISPR/Cas9

(2016) Tomoko Kato et al.   Briefings in Functional Genomics

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

(2016) Masakazu Hashimoto et al.   DEVELOPMENTAL BIOLOGY

A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes

(2016) Harumi Nakao et al.   GENESIS

Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair

(2016) Joffrey Mianné et al.   Genome Medicine

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

(2016) Yoshiko Nakagawa et al.   Biology Open

A non-inheritable maternal Cas9-based multiple-gene editing system in mice

(2016) Takayuki Sakurai et al.   Scientific Reports

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

(2015) Yoshiko Nakagawa et al.   BMC BIOTECHNOLOGY

Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse

(2015) Yoshiki Tsuchiya et al.   GENES TO CELLS

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

(2015) Tetsushi Sakuma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

(2015) Tetsushi Sakuma et al.   Nature Protocols

CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

(2015) Hiromi Miura et al.   Scientific Reports

Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos

(2015) Liren Wang et al.   Scientific Reports

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice

(2015) Toru Takeo et al.   PLoS One

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

(2015) Yu Hisano et al.   Scientific Reports

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system

(2014) Wataru Fujii et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

A Mouse Geneticist's Practical Guide to CRISPR Applications

(2014) P. Singh et al.   GENETICS

Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system

(2014) Seiya Mizuno et al.   MAMMALIAN GENOME

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

(2014) Shota Nakade et al.   Nature Communications

Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

(2014) Masafumi Inui et al.   Scientific Reports

Efficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology

(2014) Feng Li et al.   Scientific Reports

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Applications of cryopreserved unfertilized mouse oocytes for in vitro fertilization

(2013) Naomi Nakagata et al.   CRYOBIOLOGY

Translating human genetics into mouse: The impact of ultra-rapidin vivogenome editing

(2013) Tomomi Aida et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Biochemical DSB-repair model for mammalian cells in G1 and early S phases of the cell cycle

(2013) Reza Taleei et al.   MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

Osteopontin Undergoes Polymerizationin Vivoand Gains Chemotactic Activity for Neutrophils Mediated by Integrin α9β1

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