Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system

(2014) Wataru Fujii et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Screening Methods to Identify TALEN-Mediated Knockout Mice

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

Dual sgRNAs facilitate CRISPR/Cas9-mediated mouse genome targeting

(2014) Jiankui Zhou et al.   FEBS Journal

A Mouse Geneticist's Practical Guide to CRISPR Applications

(2014) P. Singh et al.   GENETICS

One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System

(2014) Wataru FUJII et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

(2014) Bin Shen et al.   NATURE METHODS

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering

(2014) Takuro Horii et al.   Scientific Reports

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

(2014) Akihiro Yasue et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Heritable gene-targeting with gRNA/Cas9 in rats

(2013) Xinli Hu et al.   CELL RESEARCH

Applications of cryopreserved unfertilized mouse oocytes for in vitro fertilization

(2013) Naomi Nakagata et al.   CRYOBIOLOGY

Translating human genetics into mouse: The impact of ultra-rapidin vivogenome editing

(2013) Tomomi Aida et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes

(2013) Daisuke Mashiko et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

(2013) Wataru Fujii et al.   NUCLEIC ACIDS RESEARCH

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

(2013) Tetsushi Sakuma et al.   Scientific Reports