Review
Developmental Biology
Lihua Ye, John F. Rawls
Summary: Developmental programs in animals not only shape their forms, but also equip them with the ability to sense and adapt to the microbial world they evolved within. Studies in human, mouse, zebrafish and Drosophila demonstrate the significant impact of microbiota on gut development and its communication with the nervous system, which has important implications for human and animal health.
Review
Gastroenterology & Hepatology
Hannah M. Kolev, Klaus H. Kaestner
Summary: This article introduces the fascinating process of mammalian intestinal development, from its earliest origins to a complex organ system. It explains the key signaling molecules and cell types involved in this process.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Multidisciplinary Sciences
Ningbo Wu, Hongxiang Sun, Xiaoyun Zhao, Yao Zhang, Jianmei Tan, Yuanyuan Qi, Qun Wang, Melissa Ng, Zhaoyuan Liu, Lingjuan He, Xiaoyin Niu, Lei Chen, Zhiduo Liu, Hua-Bing Li, Yi Arial Zeng, Manolis Roulis, Dou Liu, Jinke Cheng, Bin Zhou, Lai Guan Ng, Duowu Zou, Youqiong Ye, Richard A. Flavell, Florent Ginhoux, Bing Su
Summary: The study identified a subset of intestinal stromal cells, MRISCs, as the primary cellular source of WNT agonist R-spondin 1 following intestinal injury in mice. These MRISCs are located at the bases of colon crypts, maintain LGR5(+) intestinal stem cells, and protect against acute intestinal damage through enhanced R-spondin 1 production. The mechanism involves a reactive oxygen species (ROS)-MAP3K2-ERK5-KLF2 axis to augment WNT signaling for intestinal regeneration.
Review
Food Science & Technology
Ning Ma, Xiyue Chen, Chunchen Liu, Yiwei Sun, Lee J. Johnston, Xi Ma
Summary: This article reviews the critical role of intestinal stem cells (ISCs) in governing the proliferation and differentiation of the intestinal epithelium, as well as the regulation of ISCs by dietary factors. Through pathways such as mTORC1, PPAR/CPT1 alpha, PPAR gamma/beta-catenin, and Wnt/GSK-3 beta, factors such as caloric restriction, fasting, fat, glucose, and trace elements in the diet affect the function and self-renewal of ISCs, and may serve as potential targets for cancer prevention. However, a lack of precise definitions of nutrition intervention hinders our understanding of the biological response of ISCs to nutrients.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Nicole Verdile, Rolando Pasquariello, Gloriana Cardinaletti, Emilio Tibaldi, Tiziana A. L. Brevini, Fulvio Gandolfi
Summary: Aquaculture is expanding due to high demand for fish consumption, prompting efforts to find sustainable alternative feeds and understand intestinal morphology and physiology. Research on intestinal stem-cell niches in fish species and the role of telocytes in intestinal homeostasis has potential to improve sustainability and growth performance in trout farming.
Review
Biochemistry & Molecular Biology
Haoming Luo, Mingxing Li, Fang Wang, Yifei Yang, Qin Wang, Yueshui Zhao, Fukuan Du, Yu Chen, Jing Shen, Qianyun Zhao, Jiuping Zeng, Shengpeng Wang, Meijuan Chen, Xiaobing Li, Wanping Li, Yuhong Sun, Li Gu, Qinglian Wen, Zhangang Xiao, Xu Wu
Summary: This article reviews the interplay between intestinal stem cells and gut microbiota in their local environment, and discusses the progress and future directions of intestinal organoid culture.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yuanlong Hou, Wei Wei, Xiaojing Guan, Yali Liu, Gaorui Bian, Dandan He, Qilin Fan, Xiaoying Cai, Youying Zhang, Guangji Wang, Xiao Zheng, Haiping Hao
Summary: The study shows that dietary raffinose metabolism to fructose couples stress-induced gut microbial remodeling to intestinal stem cell renewal and epithelial homeostasis. Dietary impact on stress and gut microbial structure and epithelial integrity could be exploited to alleviate gut disorders. Dietary modulation of microbial metabolism shapes intestinal stem cell turnover, highlighting the interplay between diet, stress, and gut health.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Costanza Zanetti, Rahul Kumar, Joscha Ender, Parimala S. Godavarthy, Mark Hartmann, Joschka Hey, Kersten Breuer, Eva S. Weissenberger, Valentina R. Minciacchi, Christina Karantanou, Zhaohui Gu, Kathryn G. Roberts, Markus Metzler, Wendy Stock, Charles G. Mullighan, Clara D. Bloomfield, Natalie Filmann, Katrin Bankov, Sylvia Hartmann, Robert P. Hasserjian, Antony F. Cousins, Christina Halsey, Christoph Plass, Daniel B. Lipka, Daniela S. Krause
Summary: The study reveals that B-cell acute lymphoblastic leukemia (B-ALL) is more common in children, whereas chronic myeloid leukemia is more frequent in adults. The age of bone marrow micro-environment (BMM) and the influence of BM macrophages play a significant role in the leukemia phenotype. The CXCR5-CXCL13 axis may serve as a prognostic marker and a potential target for the treatment of B-ALL.
Article
Cell Biology
Brisa Palikuqi, Jeremie Rispal, Ophir Klein
Summary: The intestinal epithelium undergoes continuous cellular turnover, and intestinal stem cells (ISCs) can both self-renew and differentiate. Under stress conditions, intestinal epithelial cells are quite plastic, allowing for dedifferentiation and reversal of cell fate. The ISC niche, made up of various cell types, plays a central role in intestinal function.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Article
Engineering, Biomedical
Victoria Barnhouse, Nathan Petrikas, Cody Crosby, Janet Zoldan, Brendan Harley
Summary: The study developed and characterized a three-dimensional perivascular tissue model to investigate the influence of the perivascular secretome on HSC behavior, finding that perivascular conditioned media promoted maintenance of a greater fraction of hematopoietic stem and progenitor cells within a 4-day culture period.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Review
Cell & Tissue Engineering
Zi-Yan Xu, Jin-Jian Huang, Ye Liu, Yun Zhao, Xiu-Wen Wu, Jian-An Ren
Summary: The development of mini-guts organoid, derived from the identification of Lgr5(+) intestinal stem cells and their surrounding signals, provides a high-performance and physiological platform for intestinal research and therapy. However, limitations such as canonical culture pattern hinder the expanding applications of organoids. Breakthroughs in cellular biology and bioengineering contribute to diversifying the reconstitution of stem cell niches.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Cell Biology
Claudia A. Rivera, Ana-Maria Lennon-Dumenil
Summary: This article introduces the intestine as the body's largest proportion of immune cells, continuously exposed to new antigens and immune stimuli from the diet, microbiota, and intestinal pathogens. It describes the main populations of immune cells present in the intestine from both the innate and adaptive immune system. The article later discusses how the intestinal niches significantly impact the phenotype and function of gut immune populations during steady state and infection.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Immunology
Jinzhi Wang, Shuang Zheng, Xin Yang, Ben Huazeng, Qian Cheng
Summary: This study demonstrates the impact of microbial dysbiosis associated with non-IgE-mediated cow's milk-induced proctocolitis on intestinal Treg cells and immune homeostasis, leading to an overactivated Th2 biased immune response. Specific gut microbial phylotypes potentially responsible for this disruption have been identified.
MICROBIAL PATHOGENESIS
(2021)
Article
Medicine, Research & Experimental
Danni Yu, Quanhui Dai, Zixiang Wang, Steven X. Hou, Ling V. Sun
Summary: This study aimed to explore the role of ARF1 in intestinal epithelial cells (IECs) and reveal the possible mechanism. The results showed that ARF1 is essential for ISC proliferation and differentiation. Loss of ARF1 increased susceptibility to DSS-induced colitis and gut microbial dysbiosis. Additionally, RNA-seq analysis revealed alterations in multiple metabolic pathways.
Article
Immunology
Aline Ignacio, Kathleen Shah, Jeremiah Bernier-Latmani, Yasmin Koeller, Gillian Coakley, Mati Moyat, Romain Hamelin, Florence Armand, Nick C. Wong, Hena Ramay, Carolyn A. Thomson, Regula Burkhard, Haozhe Wang, Antoine Dufour, Markus B. Geuking, Braedon McDonald, Tatiana Petrova, Nicola L. Harris, Kathy D. McCoy
Summary: This study reveals the important role of intestinal eosinophils in the mutualistic interactions between the host and microbiota. Eosinophil-deficient mice showed various abnormalities including villus blunting, altered extracellular matrix, decreased epithelial cell turnover, increased gastrointestinal motility, and decreased lipid absorption.
Article
Gastroenterology & Hepatology
Bailey C. E. Peck, Matthew Weiser, Saangyoung E. Lee, Gregory R. Gipson, Vishal B. Iyer, Ryan B. Sartor, Hans H. Herfarth, Millie D. Long, Jonathan J. Hansen, Kim L. Isaacs, Dimitri G. Trembath, Reza Rahbar, Timothy S. Sadiq, Terrence S. Furey, Praveen Sethupathy, Shehzad Z. Sheikh
INFLAMMATORY BOWEL DISEASES
(2015)
Article
Biochemistry & Molecular Biology
Bailey C. E. Peck, John Sincavage, Sydney Feinstein, Amanda T. Mah, James G. Simmons, P. Kay Lund, Praveen Sethupathy
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Sara Irani, Xiaoyue Pan, Bailey C. E. Peck, Jahangir Iqbal, Praveen Sethupathy, M. Mahmood Hussain
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Ajeet P. Singh, Julie F. Foley, Mark Rubino, Michael C. Boyle, Arpit Tandon, Ruchir Shah, Trevor K. Archer
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Bailey C. E. Peck, Amanda T. Mah, Wendy A. Pitman, Shengli Ding, P. Kay Lund, Praveen Sethupathy
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Oncology
Ajeet Pratap Singh, Julie Foley, Arpit Tandon, Dhiral Phadke, H. Karimi Kinyamu, Trevor K. Archer
Article
Endocrinology & Metabolism
C. Lisa Kurtz, Bailey C. E. Peck, Emily E. Fannin, Carine Beysen, Ji Miao, Stuart R. Landstreet, Shengli Ding, Vandana Turaga, P. Kay Lund, Scott Turner, Sudha B. Biddinger, Kasey C. Vickers, Praveen Sethupathy
Article
Genetics & Heredity
Holly Rutledge, David L. Aylor, Danielle E. Carpenter, Bailey C. Peck, Peter Chines, Lawrence E. Ostrowski, Elissa J. Chesler, Gary A. Churchill, Fernando Pardo-Manuel de Villena, Samir N. P. Kelada
Article
Biochemistry & Molecular Biology
Ajeet Pratap Singh, Trevor K. Archer
NUCLEIC ACIDS RESEARCH
(2014)
Article
Developmental Biology
Jeannine M. Basta, Ajeet P. Singh, Lynn Robbins, Lisa Stout, Michelle Pherson, Michael Rauchman
DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ajeet P. Singh, Huacheng Luo, Meghana Matur, Melanie A. Eshelman, Karina Hamamoto, Arati Sharma, Julia Lesperance, Suming Huang
Summary: MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play crucial roles in regulating gene expression. The interaction between HOTTIP lncRNA and miRNAs in leukemogenesis is not well understood. Research shows that HOTTIP directly controls certain miRNA genes, impacting HOX cluster miRNAs expression and promoting leukemogenesis through chromatin remodeling. Inactivation of HOTTIP or miR-196b induces apoptosis and affects FAS gene expression in AML cells.
Article
Medicine, Research & Experimental
Ki-Suk Kim, Bailey Ce Peck, Yu-Han Hung, Kieran Koch-Laskowski, Landon Wood, Priya H. Dedhia, Jason R. Spence, Randy J. Seeley, Praveen Sethupathy, Darleen A. Sandoval
Summary: This study found that vertical sleeve gastrectomy (VSG) increases the number of hormone-secreting enteroendocrine cells (EECs) in the intestine through bile acid signaling, which drives intestinal stem cell fate towards EEC differentiation.
Article
Gastroenterology & Hepatology
Ajeet P. Singh, Yu-Han Hung, Michael T. Shanahan, Matt Kanke, Alessandro Bonfini, Michael K. Dame, Mandy Biraud, Bailey C. E. Peck, Oyebola O. Oyesola, John M. Freund, Rebecca L. Cubitt, Ennessa G. Curry, Liara M. Gonzalez, Gavin A. Bewick, Elia D. Tait-Wojno, Natasza A. Kurpios, Shengli Ding, Jason R. Spence, Christopher M. Dekaney, Nicolas Buchon, Praveen Sethupathy
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2020)