Time-gated detection of protein-protein interactions with transcriptional readout

A light- and calcium-gated transcription factor for imaging and manipulating activated neurons

(2017) Wenjing Wang et al.   NATURE BIOTECHNOLOGY

Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain

(2017) Dongmin Lee et al.   NATURE METHODS

β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle

(2016) Susanne Nuber et al.   NATURE

A split horseradish peroxidase for the detection of intercellular protein–protein interactions and sensitive visualization of synapses

(2016) Jeffrey D Martell et al.   NATURE BIOTECHNOLOGY

β-Arrestin drives MAP kinase signalling from clathrin-coated structures after GPCR dissociation

(2016) K. Eichel et al.   NATURE CELL BIOLOGY

PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome

(2015) Wesley K Kroeze et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome

(2015) Wesley K Kroeze et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Directed evolution of APEX2 for electron microscopy and proximity labeling

(2014) Stephanie S Lam et al.   NATURE METHODS

The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells

(2014) Julia Petschnigg et al.   NATURE METHODS

Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins

(2014) Gurkan Guntas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Zeitlupe Senses Blue-Light Fluence To Mediate Circadian Timing in Arabidopsis thaliana

(2013) Ashutosh Pudasaini et al.   BIOCHEMISTRY

A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast

(2013) Xiaojing Yang et al.   MOLECULAR BIOLOGY OF THE CELL

Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging

(2013) H.-W. Rhee et al.   SCIENCE

Visualization and Quantitative Analysis of G Protein-Coupled Receptor−β-Arrestin Interaction in Single Cells and Specific Organs of Living Mice Using Split Luciferase Complementation

(2012) Hideo Takakura et al.   ACS Chemical Biology

Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing

(2012) Hidehiko K. Inagaki et al.   CELL

Cell Contact-dependent Functional Selectivity of β2-Adrenergic Receptor Ligands in Stimulating cAMP Accumulation and Extracellular Signal-regulated Kinase Phosphorylation

(2012) Ali I. Kaya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy

(2012) Jeffrey D Martell et al.   NATURE BIOTECHNOLOGY

Split-protein systems: beyond binary protein–protein interactions

(2011) Sujan S Shekhawat et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

β-arrestin-mediated receptor trafficking and signal transduction

(2011) Sudha K. Shenoy et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Activation of Specific Apoptotic Caspases with an Engineered Small-Molecule-Activated Protease

(2010) Daniel C. Gray et al.   CELL

Differential Signaling of the Endogenous Agonists at the β2-Adrenergic Receptor

(2010) Susanne Reiner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid blue-light–mediated induction of protein interactions in living cells

(2010) Matthew J Kennedy et al.   NATURE METHODS

Rationally improving LOV domain–based photoswitches

(2010) Devin Strickland et al.   NATURE METHODS

Bimolecular Fluorescence Complementation (BiFC) Analysis as a Probe of Protein Interactions in Living Cells

(2008) Tom K. Kerppola   Annual Review of Biophysics