Review
Agriculture, Dairy & Animal Science
Ehsan Ahmadifar, Hamideh Pourmohammadi Fallah, Morteza Yousefi, Mahmoud A. O. Dawood, Seyed Hossein Hoseinifar, Hossein Adineh, Sevdan Yilmaz, Marina Paolucci, Hien Van Doan
Summary: Medicinal herbs as alternative additives in aquaculture have significant effects on animal performances, including promoting growth, stimulating the immune system, reducing oxidative stress, and enhancing disease resistance. However, the exact mechanisms of action of these herbs are not well understood.
Article
Fisheries
Alberto Medina, Jorge Garcia-Marquez, Miguel Angel Morinigo, Salvador Arijo
Summary: Some probiotics, such as Vibrio proteolyticus DCF12.2, have the potential to activate the immune response and produce antibodies that react with pathogens in aquaculture. The DCF12.2 strain showed the ability to activate fish immune response genes and increase antibody production against Photobacterium damselae subsp. piscicida and Vibrio harveyi. It provided protection against both pathogens, with relative percent survival (RPS) rates of 30-40% and 67% respectively. This study suggests that DCF12.2 could be used as a live vaccine for disease prevention in aquaculture.
Article
Genetics & Heredity
Hege Lund, Anne Bakke, Preben Boysen, Sergey Afanasyev, Alexander Rebl, Farah Manji, Gordon Ritchie, Aleksei Krasnov
Summary: The assessment of immune competence in farmed Atlantic salmon during smoltification and early sea life is crucial. Recent tools were used to analyze gene expression, antibody levels, and IgM transcripts in salmon from different farming systems. The study found differences in immune gene expression and antibody levels between the two cohorts, suggesting a more agitated immune system in RAS-raised salmon initially, but leveling off over time.
Article
Fisheries
Mehwish Faheem, Rafia Jamal, Nadia Nazeer, Saba Khaliq, Seyed Hossein Hoseinifar, Hien Van Doan, Marina Paolucci
Summary: The present study found that supplementation of Spirulina in the diet of grass carp has positive effects on growth, digestive enzymes, antioxidants, and innate immunity. Specifically, adding 1% and 5% Spirulina in the diet promotes growth, increases intestinal digestive enzyme activity, reduces hepatic lipid peroxidation, enhances hepatic antioxidant enzyme activity and glutathione levels, and boosts immune function in the skin mucus of fish.
Article
Fisheries
Jos'e Antonio Mata-Sotres, Carolina Flores-Salas, Arleta Krystyna Skrzynska, Aurora Tinajero, Bruno Cavalheiro Araujo, Maria Teresa Viana
Summary: This study aimed to evaluate the catabolism and retention of lipids in Seriola dorsalis juveniles under optimum levels of restriction. The lipid metabolism genes involved were determined. The results showed that saturated fatty acids were mainly oxidized, followed by monounsaturated fatty acids and linoleic acid, while long-chain polyunsaturated fatty acids were only oxidized in excess. The gene expression analysis revealed differences in the activation and uptake of fatty acids. The study suggests that 13% crude fat is the ideal amount for S. dorsalis.
Article
Agriculture, Dairy & Animal Science
Yuhao Peng, Hongjuan Shi, Yuqi Liu, Yang Huang, Renchi Zheng, Dongneng Jiang, Mouyan Jiang, Chunhua Zhu, Guangli Li
Summary: This study analyzed the gill transcriptomes of greater amberjack under different salinity stress and explored the regulatory networks of salinity-related pathways. The results provide valuable insights into the molecular basis of salinity adaptation in greater amberjack. The study also identified differentially expressed genes involved in various biological processes such as steroid biosynthesis, lipid metabolism, ion transporters, and immune response, and showed enrichment in important pathways.
Review
Food Science & Technology
A. Cimbalo, M. Frangiamone, G. Font, L. Manyes
Summary: This review highlights recent advances in using transcriptomics and proteomics as key tools to understand the molecular toxicity of mycotoxins. The most studied mycotoxin using transcriptomics is deoxynivalenol (DON), followed by aflatoxins (AFs) and zearalenone (ZEA). In contrast, proteomics mostly focuses on AFs, with some research on ZEA and DON. However, fewer studies have investigated the toxicological effects of emerging mycotoxins such as patulin, ochratoxin A, T-2 toxin, alternariol, and amino-14,16-dimethyloctadecan-3-ol. Transcriptomics commonly studies changes in gene expression related to the immune system, while proteomics focuses on cellular processes. The most commonly used techniques are RT-qPCR for gene expression analysis and liquid chromatography coupled with mass spectrometry for proteomics assays. The data gathered indicate an increased interest in using these omic approaches in the past five years. However, in vitro models are prioritized over in vivo and ex vivo models. Therefore, there is a need to enhance the use of in vivo models and alternative methods to better understand the mode of action of mycotoxins on animal and human health.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Fisheries
Jose Carlos Campos-Sanchez, Javier Mayor-Lafuente, Daniel Gonzalez-Silvera, Francisco A. Guardiola, Maria Angeles Esteban
Summary: The study evaluated the expression of inflammation-related genes in fish skin injected with carrageenin, finding it to be a good stimulator for studying skin inflammation in gilthead seabream. This method could potentially be used to study the modulation of fish inflammatory processes caused by internal or external factors.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sandra Garcia-Mulero, Maria Henar Alonso, Luis P. del Carpio, Rebeca Sanz-Pamplona, Josep M. Piulats
Summary: Uveal melanoma (UM) is a common malignant eye tumor with poor prognosis, characterized by a unique immunosuppressive microenvironment. Our study found that high immune and stromal scores in the UM microenvironment were associated with poor prognosis, while lymphocytic infiltration was related to poor prognosis and only B cells were associated with a more favorable prognosis. Samples with high angiogenic and inflammatory phenotypes exhibited poor prognosis and metabolic activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Wei Wang, Taja Lozar, Athena E. Golfinos, Denis Lee, Ellery Gronski, Ella Ward-Shaw, Mitchell Hayes, Justine Y. Bruce, Randall J. Kimple, Rong Hu, Paul M. Harari, Jin Xu, Aysenur Keske, Paul M. Sondel, Megan B. Fitzpatrick, Huy Q. Dinh, Paul F. Lambert
Summary: This study investigates the association between high expression of stress keratin 17 (K17) and poor survival and resistance to immunotherapy in human head and neck squamous cell carcinoma (HNSCC). Through the use of a mouse model and patients with HNSCC, it is found that K17 expression plays an important role in regulating the tumor microenvironment and immune responsiveness. Knocking out K17 results in slower tumor growth, increased infiltration of immune cells, and elimination of tumors upon immune-checkpoint blockade treatment. High K17 expression is associated with poor response to pembrolizumab treatment in HNSCC patients.
CLINICAL CANCER RESEARCH
(2022)
Article
Environmental Sciences
Karina Godoy, Cristian Sandoval, Claudio Vasquez, Carlos Manterola-Barroso, Barbara Toledo, Joel Calfuleo, Carolina Beltran, Marion Bustamante, Sebastian Valderrama, Mariana Rojas, Luis A. Salazar
Summary: In this study, we evaluated the gene expression during bone formation and regeneration and their relationship with mineralization in rainbow trout with mandibular deformation. The results showed marked elevation of bone morphogenetic protein 2 (Bmp2) and distinct expression patterns for transcriptional factors. This deformation was characterized by demineralization, increased porosity, and a moderate decrease in bone mineral content.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Cell Biology
Hairui Fan, Zhanshi Ren, Chao Xu, Haifei Wang, Zhengchang Wu, Zia Ur Rehman, Shenglong Wu, Ming-an Sun, Wenbin Bao
Summary: Deoxynivalenol (DON) is a common environmental toxin secreted by fusarium fungi, which can induce cytotoxic effects and disrupt hormone secretion in ovarian granulosa cells (GCs). The effects may involve alterations in multiple signaling pathways and genes.
Article
Pharmacology & Pharmacy
T. A. Reis, S. M. Tralamazza, E. Coelho, P. Zorzete, D. I. T. Favaro, B. Correa
Summary: This study investigated the production of aflatoxins by Aspergillus nomiae in Brazil nuts and found that gene expression of the aflatoxin biosynthesis pathway in A. nomiae starts before toxin production. These findings are important for predicting the production of aflatoxins in Brazil nuts.
Article
Microbiology
Manon Fallet, Caroline Montagnani, Bruno Petton, Luc Dantan, Julien de Lorgeril, Sebastien Comarmond, Cristian Chaparro, Eve Toulza, Simon Boitard, Jean-Michel Escoubas, Agnes Vergnes, Jacqueline Le Grand, Ingo Bulla, Yannick Gueguen, Jeremie Vidal-Dupiol, Christoph Grunau, Guillaume Mitta, Celine Cosseau
Summary: This study found that early microbial exposure in Pacific oysters can significantly improve their survival rate against a pathogen causing mortality syndrome. The protection provided by microbial exposure is inheritable across generations and is associated with changes in epigenetic marks and immune gene expression. This discovery suggests that microbial exposure may be an important strategy for protecting oyster aquaculture from infectious diseases.
Article
Fisheries
Piyatida Outama, Chinh Le Xuan, Supreya Wannavijit, Chompunut Lumsangkul, Nguyen Vu Linh, Napatsorn Montha, Sudaporn Tongsiri, Chanagun Chitmanat, Hien Van Doan
Summary: This study investigated the effects of mango peel powder (MGPP) on the growth, innate immunity, and immune-antioxidant related gene expression of Nile tilapia. The results showed that dietary inclusion of MGPP 25 significantly enhanced immune response and immune related gene expressions, but had no significant effect on growth performance and antioxidant gene expressions. These findings suggest that MGPP could be used as an immunostimulant in Nile tilapia culture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
C. Pietsch, P. Burkhardt-Holm
WORLD MYCOTOXIN JOURNAL
(2015)
Article
Food Science & Technology
Constanze Pietsch, Susanne Kersten, Hana Valenta, Sven Daenicke, Carsten Schulz, Patricia Burkhardt-Holm, Ranka Junge
Article
Biotechnology & Applied Microbiology
Constanze Pietsch, Ranka Junge, Patricia Burkhardt-Holm
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Mycology
Constanze Pietsch, Barbara A. Katzenback, Erick Garcia-Garcia, Carsten Schulz, Miodrag Belosevic, Patricia Burkhardt-Holm
MYCOTOXIN RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Constanze Pietsch, Ranka Junge
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2016)
Article
Food Science & Technology
Linda Tschirren, Seraina Siebenmann, Constanze Pietsch
Article
Fisheries
Sophia Egloff, Constanze Pietsch
DISEASES OF AQUATIC ORGANISMS
(2018)
Article
Mycology
Constanze Pietsch
MYCOTOXIN RESEARCH
(2020)
Article
Food Science & Technology
Constanze Pietsch, Georg Mueller, Sulayman Mourabit, Simon Carnal, Kasun Bandara
Article
Agriculture, Dairy & Animal Science
Alexander Burren, Constanze Pietsch
Summary: The aquaculture sector plays a significant role in global seafood consumption, but lacks clear guidelines for responsible practices and animal wellbeing. Gene expression studies are important for understanding animal welfare, and this study applied statistical methods to evaluate the impact of stress on fish gene expression levels, identifying potential markers for adverse conditions.
Review
Food Science & Technology
Paraskevi Koletsi, Johan W. Schrama, Elisabeth A. M. Graat, Geert F. Wiegertjes, Philip Lyons, Constanze Pietsch
Summary: This study evaluated the occurrence of mycotoxin patterns in feedstuffs and fish feeds, and summarized the effects of DON on farmed fish species. The risk of DON exposure in fish was assessed, with results indicating that the risk of DON contamination is underestimated in the aquaculture industry. The importance of regular state-of-the-art mycotoxin analyses to prevent detrimental effects on farmed fish was emphasized.
Article
Fisheries
Paulina Pawlak, Alexander Burren, Andreas Seitz, Gaetan Glauser, Constanze Pietsch
Summary: This study investigated gene expression patterns in different brain regions of koi carp during stress responses. The results showed that various stressors can affect the expression of multiple genes, and genes related to the stress axis play a crucial role in the stress process.
AQUACULTURE RESEARCH
(2022)
Article
Green & Sustainable Science & Technology
T. Stadtlander, J. Bandy, D. Rosskothen, C. Pietsch, F. Tschudi, M. Sigrist, A. Seitz, F. Leiber
Summary: Duckweed, as a potential nitrogen collector and protein source, provides opportunities for circular economy systems. The study shows that both species of duckweed have optimal biomass and protein yield at a slurry dilution of 1:8 with a total ammonia nitrogen (TAN) concentration of 19 mg l(-1).
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Multidisciplinary Sciences
Paulina Pawlak, Alexander Burren, Andreas Seitz, Constanze Pietsch
Summary: Our current understanding of stress responses and the specific roles of different regulatory pathways is still incomplete. This study aimed to investigate the regulation of appetite genes in fish, particularly in response to different stressors. The study conducted a stress trial on koi carp and evaluated the effects of tank manipulation, air exposure, and feed rewarding on appetite gene regulation. The results revealed pronounced responses in different brain regions, including the hypothalamus, to the various stressors.
ROYAL SOCIETY OPEN SCIENCE
(2023)
