Article
Endocrinology & Metabolism
Martha Hanschkow, Nathalie Boulet, Elena Kempf, Anne Bouloumie, Wieland Kiess, Robert Stein, Antje Korner, Kathrin Landgraf
Summary: MSCA1 and CD36 are associated with obesity-related alterations in children's adipose tissue, with CD36 expression predicting the adipogenic potential of SVF cells.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Cell & Tissue Engineering
Rongmei Qu, Kai He, Tingyu Fan, Yuchao Yang, Liyao Mai, Zhiwei Lian, Zhitao Zhou, Yan Peng, Asmat Ullah Khan, Bing Sun, Xiaolan Huang, Jun Ouyang, Xinghua Pan, Jingxing Dai, Wenhua Huang
Summary: The study used single-cell RNA sequencing to investigate the chemically induced osteogenesis process of human adipose MSCs, identifying some cells intrinsically prepared for osteogenesis and a set of genes related to cell stress response.
Article
Oncology
Zheng Wang, Chengyan Chai, Rui Wang, Yimei Feng, Lei Huang, Yiming Zhang, Xia Xiao, Shijie Yang, Yunfang Zhang, Xi Zhang
Summary: This study utilized advanced single-cell RNA sequencing to construct a comprehensive atlas of over 130,000 single-MSC transcriptomes across multiple tissues and donors, revealing seven tissue-specific and five conserved MSC subpopulations with distinct gene expression signatures. The study demonstrated the significant contribution of extracellular matrix to MSC heterogeneity and identified potential improvements in MSC-based therapeutic efficacy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Multidisciplinary Sciences
Lei Han, Xiaoyu Wei, Chuanyu Liu, Giacomo Volpe, Zhenkun Zhuang, Xuanxuan Zou, Zhifeng Wang, Taotao Pan, Yue Yuan, Xiao Zhang, Peng Fan, Pengcheng Guo, Yiwei Lai, Ying Lei, Xingyuan Liu, Feng Yu, Shuncheng Shangguan, Guangyao Lai, Qiuting Deng, Ya Liu, Liang Wu, Quan Shi, Hao Yu, Yunting Huang, Mengnan Cheng, Jiangshan Xu, Yang Liu, Mingyue Wang, Chunqing Wang, Yuanhang Zhang, Duo Xie, Yunzhi Yang, Yeya Yu, Huiwen Zheng, Yanrong Wei, Fubaoqian Huang, Junjie Lei, Waidong Huang, Zhiyong Zhu, Haorong Lu, Bo Wang, Xiaofeng Wei, Fengzhen Chen, Tao Yang, Wensi Du, Jing Chen, Shibo Xu, Juan An, Carl Ward, Zongren Wang, Zhong Pei, Chi-Wai Wong, Xiaolei Liu, Huafeng Zhang, Mingyuan Liu, Baoming Qin, Axel Schambach, Joan Isern, Liqiang Feng, Yan Liu, Xiangyu Guo, Zhen Liu, Qiang Sun, Patrick H. Maxwell, Nick Barker, Pura Munoz-Canoves, Ying Gu, Jan Mulder, Mathias Uhlen, Tao Tan, Shiping Liu, Huanming Yang, Jian Wang, Yong Hou, Xun Xu, Miguel A. Esteban, Longqi Liu
Summary: Studying tissue composition and function in non-human primates is crucial for understanding the nature of human species. This study presents a large-scale cell transcriptomic atlas of the non-human primate Macaca fascicularis, providing a vast annotated resource for studying a species closely related to humans. The atlas has been used to reconstruct cell-cell interaction networks, map the distribution of receptors for viruses causing human infectious diseases, and establish potential clinical associations with human genetic diseases.
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Wee Kiat Ong, Smarajit Chakraborty, Shigeki Sugii
Summary: Human ASCs exhibit heterogeneity due to factors such as nomenclature, tissue depots, donor characteristics, species differences, study design, and tools used, with actual differences in resident cell types. Efforts to understand and standardize this heterogeneity are crucial for supporting therapeutic strategies and regulatory considerations in clinical use of ASCs.
Article
Biochemistry & Molecular Biology
Kuo-An Chu, Chang-Ching Yeh, Chun-Hsiang Hsu, Chien-Wei Hsu, Fu-Hsien Kuo, Pei-Jiun Tsai, Yu-Show Fu
Summary: This study compared the effectiveness of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) versus adipose tissue-derived mesenchymal stem cells (ADMSCs) for reversing pulmonary fibrosis in rats. The results showed that HUMSCs had better effects on lung function improvement and collagen degradation, as well as alveolar regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Andreina Bruno, Caterina Di Sano, Hans-Uwe Simon, Pascal Chanez, Angelo Maria Patti, Serena Di Vincenzo, Paola Dino, Vittoria D'Esposito, Pietro Formisano, Francesco Beguinot, Elisabetta Pace
Summary: The study found that leptin and TGF-beta directly regulate the expression of PREP1 in ASCs and mature adipocytes, with effects on immune receptors and cytokines limited to mature adipocytes. This suggests that modulating immune responses may depend on the differentiation state of adipose-derived stem cells. Further research is needed to understand the potential for new therapeutic approaches in obesity-related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Luminita Labusca, Dumitru-Daniel Herea, Anca Emanuela Minuti, Cristina Stavila, Camelia Danceanu, Petru Plamadeala, Horia Chiriac, Nicoleta Lupu
Summary: Iron oxide based magnetic nanoparticles (MNP) have versatile applications in biology and medicine. This study compared the chondrogenic conversion of ADSC-MNP and WJMSC under different conditions, finding that ADSC-MNP displayed superior proliferation and chondrogenic potential, with MF exposure further enhancing chondrogenesis in ADSC-MNP. Loading ADSC with MNP may be a promising approach for cartilage engineering in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cadenas-Martin Marta, Moratilla Adrian, Fernandez-Delgado Jorge, Arnalich-Montiel Francisco, Maria P. De Miguel
Summary: Corneal disease affects millions worldwide and standard treatment involves transplantation, but there is a shortage. Lamellar endothelial keratoplasty and directed differentiation of adult stem cells show promise in addressing the issue. Efforts are ongoing to find new sources of corneal endothelial cells for transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Andrew D. Hildreth, Feiyang Ma, Yung Yu Wong, Ryan Sun, Matteo Pellegrini, Timothy E. O'Sullivan
Summary: This study provides a comprehensive analysis of the cellular composition in healthy and obese human white adipose tissue, revealing changes in immune cell populations and inflammatory pathways in obese individuals. The findings highlight the importance of immune cell regulation in metabolic diseases and offer insights into new cellular networks and differentiation trajectories in white adipose tissue.
Article
Veterinary Sciences
Faezeh Rezaei, Samira Khasaf, Samaneh Ghasemi, Abbas Parham, Pezhman Mirshokraei
Summary: This study evaluates the impact of different conditions on MSCs derived from adipose tissue and finds suitable transportation methods to minimize changes in MSCs.
BMC VETERINARY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Shiva Shariati, Ehsan Seyedjafari, Fatemeh Sadat Mahdavi, Amirhosein Maali, Elaheh Ferdosi-Shahandashti
Summary: In this study, NiFe2O4/ZnO-coated PLLA scaffold was used to evaluate the osteogenic differentiation capability of hAMSCs. The results showed that the NiFe2O4/ZnO-coated PLLA scaffold could enhance the adhesion, proliferation, and differentiation of hAMSCs, as well as promote the expression of osteogenic markers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nutrition & Dietetics
Paul Petrus, Peter Arner
PROCEEDINGS OF THE NUTRITION SOCIETY
(2020)
Article
Endocrinology & Metabolism
Mikael Ryden, Hui Gao, Peter Arner
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Medicine, General & Internal
B. M. Lichtenberger, M. Kasper
Summary: Healthy tissues contain cells with cancer-causing mutations, but the immediate environment of these mutant cells greatly influences their potential for malignancy. Understanding the cellular heterogeneity and interactions in health and disease is crucial for developing new cancer therapies.
JOURNAL OF INTERNAL MEDICINE
(2021)
Article
Endocrinology & Metabolism
Regine A. Jersin, Divya Sri Priyanka Tallapragada, Andre Madsen, Linn Skartveit, Even Fjaere, Adrian McCann, Laurence Dyer, Aron Willems, Jan-Inge Bjune, Mona S. Bjune, Villy Vage, Hans Jorgen Nielsen, Havard Luong Thorsen, Bjorn Gunnar Nedrebo, Christian Busch, Vidar M. Steen, Matthias Blueher, Peter Jacobson, Per-Arne Svensson, Johan Ferno, Mikael Ryden, Peter Arner, Ottar Nygard, Melina Claussnitzer, Stale Ellingsen, Lise Madsen, Jorn V. Sagen, Gunnar Mellgren, Simon N. Dankel
Summary: This study reveals that the small neutral amino acid transporter SLC7A10 plays a significant role in regulating adipocyte resilience to nutrient and oxidative stress, reducing reactive oxygen species generation and lipid accumulation, and improving mitochondrial respiration, ultimately leading to decreased adipocyte hypertrophy, insulin resistance, and type 2 diabetes.
Article
Oncology
Yuan-Hung Lo, Kevin S. Kolahi, Yuhong Du, Chiung-Ying Chang, Andrey Krokhotin, Ajay Nair, Walter D. Sobba, Kasper Karlsson, Sunny J. Jones, Teri A. Longacre, Amanda T. Mah, Bahar Tercan, Alexandra Sockell, Hang Xu, Jose A. Seoane, Jin Chen, Ilya Shmulevich, Jonathan S. Weissman, Christina Curtis, Andrea Califano, Haian Fu, Gerald R. Crabtree, Calvin J. Kuo
Summary: Mutations in ARID1A are common in human cancer, but the oncogenic consequences of these mutations remain unclear. Using CRISPR/Cas9, researchers induced dysplasia, tumorigenicity, and mucinous differentiation in human gastric organoids, providing insights into the pathways affected by ARID1A mutation. Further analysis showed vulnerability of ARID1A-deficient organoids to inhibition of BIRC5/survivin, highlighting this pathway as crucial for early-stage gastric tumorigenesis.
Article
Biology
Carl Herdenberg, Pascal M. Mutie, Ola Billing, Ahmad Abdullah, Rona J. Strawbridge, Ingrid Dahlman, Simon Tuck, Camilla Holmlund, Peter Arner, Roger Henriksson, Paul W. Franks, Hakan Hedman
Summary: Herdenberg et al. demonstrate that adipogenesis and BMP signaling are disrupted in mouse cells lacking LRIG (Leucine-rich repeats and immunoglobulin-like domains) proteins. They also show that mutant LRIG/sma-10 variant worms exhibit defects in lipid storage and human LRIG1 variants are linked to higher body mass index while protecting against type 2 diabetes. This study suggests a conserved role of LRIG proteins in lipid metabolism and BMP signaling across different species.
COMMUNICATIONS BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Pauline Morigny, Jeremie Boucher, Peter Arner, Dominique Langin
Summary: White adipocyte metabolism plays a crucial role in systemic metabolism regulation and is often disrupted in conditions like cancer and type 2 diabetes mellitus. This review provides an overview of lipid metabolism in white adipocytes and discusses how these pathways can serve as therapeutic targets in metabolic disorders.
NATURE REVIEWS ENDOCRINOLOGY
(2021)
Article
Biochemical Research Methods
Anton J. M. Larsson, Christoph Ziegenhain, Michael Hagemann-Jensen, Bjorn Reinius, Tina Jacob, Tim Dalessandri, Gert-Jan Hendriks, Maria Kasper, Rickard Sandberg
Summary: This study investigates the relationship between monoallelic gene expression and transcriptional bursting based on single-cell RNA analysis, demonstrating that transcriptional bursting process can explain the amount of monoallelic expression. The burst frequency largely determines the frequency of monoallelic observations, while the burst size has minimal impact on monoallelic observations. The findings reinforce the idea that studies of allelic imbalance can be affected by the inherent noise in transcriptional bursting.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Enrichetta Mileti, Kelvin H. M. Kwok, Daniel P. Andersson, Anthony Mathelier, Amitha Raman, Jesper Backdahl, Jutta Jalkanen, Lucas Massier, Anders Thorell, Hui Gao, Peter Arner, Niklas Mejhert, Carsten O. Daub, Mikael Ryden
Summary: Selective hepatic insulin resistance is a feature of obesity and type 2 diabetes. This study showed that white adipose tissue in obese individuals also displays a selective insulin response, which is normalized by weight loss in most genes.
Article
Endocrinology & Metabolism
Christel Bjork, Narmadha Subramanian, Jianping Liu, Juan Ramon Acosta, Beatriz Tavira, Anders B. Eriksson, Peter Arner, Jurga Laurencikiene
Summary: This study conducted a comprehensive analysis of TFs affected by metabolic conditions in human WAT, revealing their significant roles in adipogenesis and identifying JARID2 as a crucial TF. These findings provide important insights into the understanding of adipocyte generation and adipose tissue expansion.
Article
Cell Biology
Katie Cockburn, Karl Annusver, David G. Gonzalez, Smirthy Ganesan, Kailin R. Mesa, Kyogo Kawaguchi, Maria Kasper, Valentina Greco
Summary: In the adult epidermis of the skin, there are molecularly heterogeneous subpopulations within the stem cell compartment. These subpopulations undergo a series of transcriptional changes to gradually differentiate into terminally differentiated cells.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sara Gallini, Karl Annusver, Nur-Taz Rahman, David G. Gonzalez, Sangwon Yun, Catherine Matte-Martone, Tianchi Xin, Elizabeth Lathrop, Kathleen C. Suozzi, Maria Kasper, Valentina Greco
Summary: Healthy skin consists of a mixture of wild-type and mutant clones. After injury, wild-type cells suppress the abnormal growth induced by mutant Ras cells through the activation of the EGFR pathway. This injury-induced switch in competitive balance prevents the expansion of oncogenic mutant cells.
Article
Multidisciplinary Sciences
Oliver J. Mainwaring, Holger Weishaupt, Miao Zhao, Gabriela Rosen, Anna Borgenvik, Laura Breinschmid, Annemieke D. Verbaan, Stacey Richardson, Dean Thompson, Steven C. Clifford, Rebecca M. Hill, Karl Annusver, Anders Sundstrom, Karl O. Holmberg, Maria Kasper, Sonja Hutter, Fredrik J. Swartling
Summary: In this study, a transgenic mouse model of Group 3 medulloblastoma was generated by MYC overexpression, and it was shown that MYC suppresses ARF to drive tumorigenesis. Computational models and clinical data were used to identify drugs targeting MYC-driven tumors with a suppressed but functional ARF pathway. CDKN2A loss and p53 mutations were rare in MYC-driven Group 3 medulloblastomas.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Oncology
Yuan-Hung Lo, Kevin S. Kolahi, Yuhong Du, Chiung-Ying Chang, Andrey Krokhotin, Ajay Nair, Walter D. Sobba, Kasper Karlsson, Sunny J. Jones, Teri A. Longacre, Amanda T. Mah, Alexandra Sockell, Jose A. Seoane, Jin Chen, Jonathan S. Weissman, Christina Curtis, Andrea Califano, Haian Fu, Gerald R. Crabtree, Calvin J. Kuo
Correction
Endocrinology & Metabolism
Agne Kulyte, Veroniqa Lundback, Cecilia M. Lindgren, Jian'an Luan, Luca A. Lotta, Claudia Langenberg, Peter Arner, Rona J. Strawbridge, Ingrid Dahlman
MOLECULAR METABOLISM
(2020)