Review
Chemistry, Multidisciplinary
Wenpeng Liu, Fei Yue, Luke P. Lee
Summary: The global outbreaks of deadly infectious diseases have urged the need for rapid and accurate pathogen detection. While nucleic acid amplification tests provide a shorter turnaround time to identify pathogens, the complex sample preparation steps remain a bottleneck for modern molecular diagnostic devices.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Biophysics
Hau Van Nguyen, Vu Minh Phan, Tae Seok Seo
Summary: The portable genetic analyzer presented is equipped with an integrated centrifugal disc for analyzing major feline upper respiratory tract disease pathogens, with the capability of performing loop-mediated isothermal amplification (LAMP) or polymerase chain reaction (PCR) within 1.5 hours.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Yujia Liu, Lawrence Kulinsky, Roya Shiri, Marc Madou
Summary: In this study, a novel method of inward pumping using elastic membranes was introduced to centrifugal microfluidic platforms, allowing the liquid to be returned from the periphery to the center of the disk. By controlling the disk's angular velocity and the fluidic resistance in two channels, efficient inward pumping was achieved, demonstrating enhanced fluid mixing and the utility for multi-step assays on the disk.
Article
Chemistry, Analytical
Hau Van Nguyen, Vu Minh Phan, Tae Seok Seo
Summary: The proposed prototype portable genetic analyzer can perform RNA extraction, RT-LAMP amplification, and fluorescence detection for ten samples simultaneously in an automatic and high-throughput manner. It utilizes zigzag aliquoting channels and passive valves for sample partitioning and extraction operations.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Peter Juelg, Elena Kipf, Mara Specht, Marion Fillies, Cornelia Eckert, Nils Paust, Roland Zengerle, Michael Lehnert, Tobias Hutzenlaub
Summary: This study introduces an automated, highly sensitive, and multiplexed qPCR quantification method using centrifugal microfluidics, showing promising results in clinical applications for disease monitoring. The approach demonstrated high accuracy in generating standard curves, specificity controls, and quantifying patient samples, providing a potential solution for reproducible gene quantification in moderate-throughput settings.
Article
Chemistry, Applied
Wenjie Yu, Yanjing Chen, Zhiying Wang, Lu Qiao, Ruibin Xie, Juan Zhang, Suying Bian, Hui Li, Yan Zhang, Ailiang Chen
Summary: A real-time fluorescent multiplex LAMP assay based on a centrifugal microfluidic chip was developed to simultaneously detect milk from cow, camel, horse, goat, and yak. The assay simplifies the detection process, reduces false-positive results, and has a genomic detection limit that meets the authentication requirements for detecting and differentiating common milk species.
Article
Biophysics
Hanh An Nguyen, Nae Yoon Lee
Summary: A paper device integrated with a power-free DNA detection strategy using polydopamine aggregation has been developed for detecting pathogens with loop-mediated isothermal amplification (LAMP). This device enables the naked-eye detection of infectious pathogens within a short period of time, making it ideal for resource-limited environments.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
T. Mounika, P. S. Girish, M. Shashi Kumar, Aparana Kumari, Sujatha Singh, Nagappa S. Karabasanavar
Summary: The study developed a rapid sheep meat identification technique using DNA extraction and LAMP technology with high specificity and sensitivity. The detection method is simple, rapid, suitable for under-resourced laboratories, and can be used for sheep meat authentication in a short period of time.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Chemistry, Analytical
Amin Dehghan, Ali Gholizadeh, Mahdi Navidbakhsh, Hossein Sadeghi, Esmail Pishbin
Summary: Micromixers are essential microfluidic modules for fabricating integrated lab-on-chip devices for point-of-care applications. In this study, a magnetic stirrer on a rotating micro-structured disk is presented to mix liquids of a wide range of viscosities in low rotational velocities. The on-disk magnetic stirrer can be implemented in integrated micmfluidic platforms without affecting other modules, and it is a convenient choice for the cell lysis process due to high mixing indexes and considerable viscous stresses.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Ruben R. G. Soares, Ahmad S. Akhtar, Ines F. Pinto, Noa Lapins, Donal Barrett, Gustaf Sandh, Xiushan Yin, Vicent Pelechano, Aman Russom
Summary: This study aimed to develop a cost-effective viral load detection system for point-of-care COVID-19 diagnostics. By developing an integrated modular centrifugal microfluidic platform, rapid amplification and detection of viral RNA from nasopharyngeal swab samples with high specificity and sensitivity were achieved.
Article
Biochemistry & Molecular Biology
Xiangyu Xiong, Jieping Liang, Zhenxiang Li, Ben-Qiang Gong, Jian-Feng Li
Summary: The study introduces a gene activator called dCas9-TV, which can simultaneously upregulate the OsGW7 and OsER1 genes in rice, achieving multi-gene coactivation and demonstrating widely applicable strategies for boosting transcriptional activation efficiency in plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Igor P. Oscorbin, Georgiy Yu. Shevelev, Ksenia A. Pronyaeva, Andrey A. Stepanov, Darya V. Shamovskaya, Olga V. Mishukova, Dmitrii V. Pyshnyi, Maksim L. Filipenko
Summary: Loop-mediated isothermal amplification (LAMP) is a stable nucleic acid amplification method that is resistant to DNA amplification inhibitors, allowing for multiplexing and suitable for SARS-CoV-2 viral RNA detection with high accuracy and short analysis time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Hau Van Nguyen, Tae Seok Seo
Summary: The novel centrifugal disc is capable of purifying nucleic acid in a high-throughput manner, processing 30 forensic samples in a single run. It automatically distributes washing solution and elution buffer, featuring a superhydrophobic zigzag-shaped structure for efficient division. The entire process is operated by a portable workstation, enabling quick completion of genetic sex-typing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Mei Xie, Tianlan Chen, Xiong Xin, Zongwei Cai, Cheng Dong, Bo Lei
Summary: A method using a digital microfluidic chip and loop-mediated isothermal amplification technology was developed for multiplex detection of foodborne pathogens. The method showed higher sensitivity, simpler operation, higher efficiency, and smaller size compared to the commercial benchtop LAMP method.
Article
Biophysics
Yujin Xiao, Shunji Li, Zheng Pang, Chao Wan, Lina Li, Huijuan Yuan, Xianzhe Hong, Wei Du, Xiaojun Feng, Yiwei Li, Peng Chen, Bi-Feng Liu
Summary: MDCM technology allows rapid and accurate dispensing of multiple reagents and samples, making it a high-potential platform for POCT.
BIOSENSORS & BIOELECTRONICS
(2022)