Journal
RSC ADVANCES
Volume 7, Issue 52, Pages 32508-32517Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra03752j
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Funding
- National Natural Science Foundation of China [21374022, 31470764]
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alpha-Synuclein (alpha-Syn) aggregates are the major component of Lewy bodies (LB), which is a pathological hallmark in the brain tissue of Parkinson's disease (PD) patients. It has been reported that (-)-epigallocatechin-3-gallate (EGCG) is biologically able to penetrate the blood-brain barrier and inhibit the fibrillation of amyloid proteins. This study aimed to provide insight into the possible mechanism of EGCG as a potential candidate agent for the prevention and treatment of PD on the basis of the interaction between a-Syn and EGCG. In the present study, the effects of EGCG on the fibrillation and disaggregation of alpha-Syn were investigated by thioflavin T (ThT) fluorescence spectroscopy, circular dichroism spectroscopy (CD), nuclear magnetic resonance (NMR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM) on a molecular level. In addition, on the cellular level, we investigated the protective effects of EGCG on alpha-Syn-induced cell death in the transduced PC12 cells which overexpressed alpha-Syn, using the techniques of 3-(4,5-dimethylthiazol-2-yl)2,5- diphenyltetrazolium bromide (MTT) assay, 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) assay, western blot and confocal laser scanning microscopy. It was found that EGCG not only significantly inhibited the conformational transition of alpha-Syn from random coil to beta-sheet conformers through binding to Ile, Phe and Tyr amino residues, but also disaggregated the amyloid fibrils of alpha-Syn in a dose-dependent manner, through binding to Leu, His, Phe and Tyr amino residues. It is also demonstrated that EGCG can protect PC12 cells against alpha-Syn-induced damage by inhibiting the overexpression and fibrillation of alpha-Syn in the cells.
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