Article
Biochemistry & Molecular Biology
Nastaran Moradi, Samad Muhammadnejad, Hamid Delavari, Negin Pournoori, Mohammad Ali Oghabian, Hossein Ghafouri
Summary: The conjugation of anti-CD3 monoclonal antibody with Fe3O4 coated by carboxymethyl dextran (CMD) using cyanogen bromide (CNBr) was shown to efficiently separate CD3+ T lymphocytes from whole blood with high purity. The method can be utilized for cell sorting and targeted MRI imaging in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Jin-Bao Tang, Hong-Ming Yang, Xiao-Yi Gao, Xian-Zhong Zeng, Feng-Shan Wang
Summary: A novel antibody conjugation strategy was developed to immobilize antibodies in a directional and covalent manner, significantly improving the sensitivity of solid-phase immunoassay. This approach can be used for surface functionalization to detect various biomarkers with increased sensitivity.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Biomedical
Marcos Gil-Garcia, Salvador Ventura
Summary: The study presents a novel approach to create highly functional, non-amyloid, and fluorescent inclusion bodies (IBs) using natural coiled-coil self-assembly properties. These protein-based nanoparticles effectively capture antibodies and are utilized for specific and simultaneous targeting of two unrelated cell types, potentially valuable in bioimaging and immunotherapy applications.
ACTA BIOMATERIALIA
(2021)
Article
Pharmacology & Pharmacy
Vineela Parvathaneni, Snehal K. Shukla, Nishant S. Kulkarni, Vivek Gupta
Summary: Repurposing old drugs for cancer treatment can provide an alternative to the challenging process of new drug discovery, with potential benefits in reducing dose and side effects. Targeting potential of transferrin-conjugated inhalable nanoparticles loaded with amodiaquine was investigated against non-small cell lung cancer, showing significant cytotoxicity and growth inhibition in cancer cells. The use of scalable nanoparticle techniques for drug delivery holds promise in improving cancer treatment outcomes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Materials Science, Multidisciplinary
Jong-Gu Choi, Mahbub Hasan, Hafeza Akter, Sang-Suk Lee
Summary: Conjugation of magnetic nanoparticles (MNPs) with biomolecules can be achieved through covalent or non-covalent bonding and chemisorption. In this study, we successfully conjugated mouse monoclonal antibody (Ab) with amine-functionalized dextran-coated MNPs by glutaraldehyde cross-linking. The characterization of the antibody-conjugated MNPs (Ab-MNPs) was conducted through buffer separation using low-gradient magnet and centrifugation.
CURRENT APPLIED PHYSICS
(2023)
Article
Medicine, Research & Experimental
Yu J. Cao, Chenfei Yu, Kuan-Lin Wu, Xuechun Wang, Dong Liu, Zeru Tian, Lijun Zhao, Xuexiu Qi, Axel Loredo, Anna Chung, Han Xiao
Summary: The pClick technology offers a simple and efficient method to prepare homogeneous antibody conjugates from native antibodies without the need for additional engineering or treatments. The resulting conjugates exhibit excellent antitumor activity and cytotoxic effects, providing a convenient strategy for developing novel antibody conjugates.
Article
Chemistry, Analytical
Yuanli Song, Jing Gao, Qian Meng, Feng Tang, Yuqiu Wang, Yue Zeng, Wei Huang, Hong Shao, Hu Zhou
Summary: Antibody-drug conjugates (ADCs) are crucial for drug development but assessing their quality attributes remains challenging due to structural heterogeneity. This study focuses on the impact of fragmentation mode on conjugation site characterization using hybrid EThcD. Results show that EThcD generates more fragment ions compared to conventional HCD, aiding in pinpointing correct conjugation sites with complex linker payloads. This study provides valuable insights for quality control of ADCs in preclinical and clinical settings.
ANALYTICA CHIMICA ACTA
(2023)
Review
Pharmacology & Pharmacy
Chawapon Pipatwatcharadate, Poornima Ramesh Iyer, Dakrong Pissuwan
Summary: This review summarizes recent developments in the use of MNPs for separating CTCs or non-CTCs in blood samples. Various approaches for magnetic-based cell separation for CTC detection, including the combination of MNPs with other particles, are discussed. Different methods for synthesizing MNPs are also included in this review.
Article
Materials Science, Multidisciplinary
Changqing Zhang, Jianwei Lin, Yunru Yu, Dawei Deng, Ying Yu, Dagan Zhang, Qifeng Zhong
Summary: The enrichment technology of circulating tumor cells (CTCs) is a medical approach used for capturing and isolating target tumor cells, which has gained significant attention for its applications in cancer diagnosis and prognosis. Magnetic nanoparticles have become an important platform for CTCs capture and isolation. However, the inert surface properties of these nanoparticles pose a challenge in analyzing the molecular phenotype of CTCs and achieving high identification sensitivity.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Nanoscience & Nanotechnology
Jiahe Liu, Xiaoyan Lyu, Ziwei Zhou, Lin Yang, Jie Zeng, Yao Yang, Zhenghuan Zhao, Rui Chen, Xin Tong, Jiaqi Li, Hailan Liu, Yuan Zou
Summary: The ability to encapsulate and manipulate droplets with a picoliter volume of samples and reagents shows great potential for practical applications in chemistry, biology, and materials science. Magnetic control is a promising approach for droplet manipulation due to its ability for wireless control and its ease of implementation. However, it is challenged by the poor biocompatibility of magnetic materials in aqueous droplets, and current droplet technology is problematic because of molecule leakage between droplets.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Medicinal
Ahmad Hussain, Armin Grimm, Wenjie Sheng, Chaoyu Zhang, Marwah Al-Rawe, Karen Braeutigam, Mobarak Abu Mraheil, Felix Zeppernick, Ivo Meinhold-Heerlein
Summary: Antibodies have revolutionized cancer treatment with their unique ability to recognize specific antigens and various therapeutic mechanisms. Modification techniques have allowed for the development of new antibody-conjugate-based diagnostic and therapeutic agents, but overcoming the heterogeneity generated by bioconjugation approaches remains a challenge. This review highlights enzyme-based site-specific conjugation methods to improve antibody properties.
Article
Pharmacology & Pharmacy
Shahram Parvaneh, Fatemeh Khademi, Gisya Abdi, Abdolhamid Alizadeh, Ali Mostafaie
Summary: This study developed new antibody-conjugated magnetic NPs for detecting HBsAg using an efficient activation and conjugation strategy, demonstrating the binding capacity comparable to commercially available products. The method designed for conjugating anti-HBsAg antibody to magnetic nanoparticles opens up the potential for producing high-capacity magnetic NPs.
Article
Chemistry, Multidisciplinary
Hongfei Chen, Hong-Chai Fabio Wong, Jiaming Qiu, Biquan Li, Dingdong Yuan, Hao Kong, Yishu Bao, Yu Zhang, Zhiyi Xu, Ying-Lung Steve Tse, Jiang Xia
Summary: Targeted immunotherapies use the binding capabilities of antibodies to enhance immune responses for long-term tumor destruction. A chemoenzymatic reaction has been developed to specifically modify a single tyrosine residue in therapeutic antibodies, allowing the attachment of fluorescent molecules or bioorthogonal groups. This reaction can be used to construct monofunctionalized antibody-drug conjugates and antibody/nanobody-conjugated effector cells, improving targeted cancer immunotherapy outcomes.
Article
Chemistry, Multidisciplinary
Mona Atabakhshi-Kashi, Monica Carril, Hossein Mahdavi, Wolfgang J. Parak, Carolina Carrillo-Carrion, Khosro Khajeh
Summary: The successful conjugation of antibodies onto a fluorescent NP assembly demonstrated that the NP assembly retained the targeting ability of the antibodies in cellular uptake, while also preserving its intrinsic properties such as fluorescence.
Review
Environmental Sciences
Nisar Ali, Buame Jacinta Hellen, Chuansong Duanmu, Yong Yang, Shahid Nawaz, Adnan Khan, Farman Ali, Xiaoyan Gao, Muhammad Bilal, Hafiz M. N. Iqbal
Summary: The use of magnetic nanoparticles in treating hydrocarbon-contaminated wastewater is considered effective, with potential for use as demulsifiers. These materials offer the ability to separate emulsions efficiently and save resources, although they face several challenges in practical application.