Article
Food Science & Technology
Lifang Zou, Xia Yu, Kezhou Cai, Baocai Xu, Conggui Chen, Guiran Xiao
Summary: This study identified six antioxidant peptides from in vitro digestion products of pork sausage that could activate the Keap1-Nrf2-ARE pathway, with DWWGSTVR showing the strongest activity. Further research demonstrated that DWWGSTVR can facilitate Nrf2 transfer to the nucleus and increase the expression of antioxidant enzymes. These peptides can potentially be used as functional foods for preventing and treating oxidative stress-induced diseases.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Martiniano Bello
Summary: Experimental findings have suggested that the antioxidative and protective activities of silymarin (SM) extract could be due to their ability to activate nuclear factor erythroid 2-related factor 2 (Nrf2). However, information about the structural and energetic basis of the inhibitory mechanism of SM compounds on the Nrf2-Keap1 pathway is lacking. This study evaluated the binding properties of SM compounds and predicted that silybinA and 2,3-dehydrosilybin are potential activators of Nrf2.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2023)
Article
Food Science & Technology
Xinqi Ma, Yuqiu Li, Chengyu Lv, Bin Liu, Cuiping Yuan, Wei Huang, Quan Luo, Yechen Xiao, Chang Sun, Tiezhu Li, Jie Zhang
Summary: This study demonstrates that oxyresveratrol inhibits oxidative damage by activating the Keap-Nrf2-ARE signaling pathway, and has the potential as an antioxidant.
Article
Chemistry, Medicinal
Dilip Narayanan, Kim T. Tran, Jakob S. Pallesen, Sara M. O. Solbak, Yuting Qin, Elina Mukminova, Martina Luchini, Kristina O. Vasilyeva, Dorleta Gonzalez Chichon, Georgia Goutsiou, Cecilie Poulsen, Nanna Haapanen, Grzegorz M. Popowicz, Michael Sattler, David Olagnier, Michael Gajhede, Anders Bach
Summary: This study utilized fluorescence polarization, thermal shift assay, and surface plasmon resonance to screen 2500 small-molecule fragments, resulting in the identification of 28 high-priority hits. The binding modes of these hits were validated through saturation transfer difference NMR, providing insights into the development of novel small-molecule Keap1-Nrf2 protein-protein interaction inhibitors.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Huijie Chen, Jianqing Chen, Xu Shi, Lu Li, Shiwen Xu
Summary: Naringenin (NRG) inhibits oxidative stress and mitochondrial apoptosis induced by BPA in swine testis (ST) cells by targeting Keap1/Nrf2 signaling pathway.
Article
Cell Biology
Tahar Aboulkassim, Xiaohong Tian, Qiang Liu, Dinghong Qiu, Mark Hancock, Jian Hui Wu, Gerald Batist
Summary: NRF2 is a regulator of protective responses in healthy tissues, but its activation in tumor cells can lead to drug resistance. The study introduces a small-molecule NRF2 inhibitor, R16, which selectively binds KEAP1 mutants and restores their NRF2-inhibitory function. This enhances the sensitivity of tumor cells with KEAP1 mutations to chemotherapy drugs. Furthermore, the authors develop a biosensor system to detect and classify KEAP1 mutations.
Article
Biochemistry & Molecular Biology
Mingjie Zhou, Wenzhu Zhao, Wenjun Xue, Jingbo Liu, Zhipeng Yu
Summary: This study clarified the antihypertensive mechanisms of QIGLF on spontaneously hypertensive rats by using a serum proteomic approach. QIGLF was found to potentially exert antihypertensive effects by inhibiting Na+ reabsorption and oxidative stress, and restoring gap junction and tight junction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Feilong Chen, Mei Xiao, Jing Feng, Reziyamu Wufur, Keli Liu, Shaofan Hu, Yiguo Zhang
Summary: Nrf2 and Keap1 play crucial roles in cellular antioxidant, detoxification, cytoprotective responses, as well as cancer metabolism. The different isoforms of Keap1 have distinct inhibitory effects on Nrf2 and its target genes, and they also possess similar but different functions as revealed by transcriptome sequencing and multiple in vivo and in vitro experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Federica Tonolo, Alessandro Grinzato, Alberto Bindoli, Maria Pia Rigobello
Summary: The increasing need for redox imbalance in chronic diseases has led to a focus on compounds with antioxidant activity. Bioactive peptides, as natural molecules with health-promoting effects, have gained interest. Recent studies have explored the effects of bioactive peptides in the cellular environment and found that they can modulate specific pathways. This review focuses on the structure-function correlation of antioxidant bioactive peptides, considering their molecular mechanism and the activation of specific signaling pathways. The correlation between molecular docking analysis and cellular effects is highlighted. This knowledge is important for proposing the use of bioactive peptides in functional foods or nutraceuticals.
Article
Chemistry, Medicinal
Mengjiao Shi, Jian Zhang, Miaomiao Li, Yaping Zhao, Ying Guo, Jiayi Xu, Rongrong Liu, Zongfang Li, Dongmei Ren, Pengfei Liu
Summary: This study investigated the protective effects of liquiritigenin (LQ), a natural flavonoid, against oxidative hepatotoxicity and the underlying mechanism. It was found that LQ eliminated reactive oxygen species in liver cells through NRF2 activation, involving both canonical and non-canonical signaling pathways. LQ-induced NRF2 activation was partially dependent on KEAP1-C151 and was also mediated by the blockage of autophagic flux and upregulation of p62, which competitively bound with KEAP1. Overall, this study reveals a novel molecular mechanism underlying the hepatoprotection of LQ, providing new insights into the pathogenesis and therapeutic strategies for oxidative liver injury.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Yifan Xu, Jing Li, Zhiwei Lin, Weiquan Liang, Lijie Qin, Jiabin Ding, Shuqi Chen, Luqian Zhou
Summary: This study found that isorhamnetin can significantly alleviate the inflammatory response in COPD mice induced by cigarette smoke, mainly by affecting the Nrf2/Keap1 pathway. It improves lung function without discernible side effects, making it a potential drug candidate for COPD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Li Wang, Xinyi Chen, Xiang Li, Dongli Liu, Xiaojun Wang, Xiaohong Chang, Youmin Guo
Summary: This study introduces a novel therapeutic approach against COPD by altering metabolism and counteracting oxidative stress through Nrf2 activation, demonstrating significant effects on the disease progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Nutrition & Dietetics
Hui Chen, Huizhen Zheng, Tiejun Li, Qihong Jiang, Shulai Liu, Xuxia Zhou, Yuting Ding, Xingwei Xiang
Summary: This study aimed to analyze the potential effect of oyster peptide (OP) on cyclophosphamide-induced oxidative damage in the mouse intestine. The results showed that OP intake significantly improved intestinal oxidative stress and mediated this effect through the antioxidant Nrf2-Keap1 signaling pathway.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Medicinal
Xinyi Qi, Xu Zhang, Junjun Meng, Jingshuai Wu, Wei Cheng, Jian Huang, Wenhan Lin
Summary: Chemoinformatic and bioassay-guided fractionation of Junceella juncea led to the discovery of 45 briarane-type diterpenoids, including 16 new analogues. These compounds showed significant inhibition against osteoclast differentiation in BMMs. Praelolide, one of the active analogues, activated the Nrf2 signaling pathway and rescued bone loss in prednisone-induced zebrafish.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Plant Sciences
Hao Yu, Siru Yan, Meiyu Jin, Yunfei Wei, Lilei Zhao, Jiaqi Cheng, Lu Ding, Haihua Feng
Summary: The study aimed to investigate the role of aescin (Aes) in treating non-alcoholic fatty liver disease (NAFLD) and its underlying mechanism. It was found that Aes can promote autophagy, activate the Nrf2 pathway, and ameliorate lipid accumulation and oxidative stress both in vitro and in vivo. However, Aes lost its therapeutic effect on NAFLD in Atg5 and Nrf2 knockout mice.
Review
Food Science & Technology
Paz Otero, M. Carpena, P. Garcia-Oliveira, J. Echave, A. Soria-Lopez, P. Garcia-Perez, M. Fraga-Corral, Hui Cao, Shaoping Nie, Jianbo Xiao, J. Simal-Gandara, M. A. Prieto
Summary: Consumers nowadays are increasingly aware of the relationship between diet and health, leading to a preference for natural products. Seaweeds have emerged as a potential source of bioactive carbohydrates, with numerous polysaccharides arousing the interest of the scientific community due to their biological activities. Environmentally friendly extraction technologies and chemical modification methods have been developed to improve the yield, quality, and applications of seaweed polysaccharides. This review aims to compile current information about seaweed polysaccharides, including extraction and modification methods, biological activities, and future trends for their industrial applications.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Food Science & Technology
Song Li, Jielun Hu, Haoyingye Yao, Fang Geng, Shaoping Nie
Summary: This review explores the effects of different galactans on gut microbiota and the production of short-chain fatty acids. It summarizes the structural characteristics of four types of galactans and discusses the ability of gut microbiota to utilize them. The findings suggest potential applications of galactans as functional foods.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Multidisciplinary
Ting Zhang, Yuanhu Tang, Huifang Ge, Deju Zhang, Ting Li, Dongkun Cheng, Jingbo Liu, Yiding Yu
Summary: The storage temperature and time significantly impact the functional properties of egg white powder (EWP), and a low temperature of 4°C is the most suitable storage condition for prolonging the shelf-life of EWP.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agriculture, Multidisciplinary
Tongwen Huang, Ke Zhang, Junqiao Wang, Kaihong He, Xingtao Zhou, Shaoping Nie
Summary: This study found that quercetin can alleviate the hepatotoxicity caused by acrylamide generated in heat-treated foods. Experiments on mice showed that quercetin can reduce the oxidative stress levels induced by acrylamide and protect the liver from damage. The results demonstrated that quercetin suppressed acrylamide-induced ferroptosis by inhibiting oxidative stress-driven autophagy, leading to the mitigation of liver injury.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Jinjin Liu, Junyi Yin, Xiaojun Huang, Cencen Liu, Liuyun Hu, Yansheng Huang, Fang Geng, Shaoping Nie
Summary: This study aimed to remove the controversial anthraquinones from Cassia obtusifolia seed water extracts (CWEs) using baking treatment (BT), stir-frying treatment (ST), and adsorption treatment (AT). Results showed that AT had the best removal effect on total anthraquinones and increased the content of neutral sugars in CWEs. However, AT decreased the antioxidant activity of CWEs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Chunhua Chen, Yanli Zhang, Hongmei Yin, Jielun Hu, Yadong Zhong, Shaoping Nie, Mingyong Xie
Summary: This study comprehensively investigated and compared the fermentation characteristics of indigestible glucans (IGs) with different structural properties and their effects on the gut microbiota. The results showed that different IGs were utilized by the gut microbiota at varying rates, and some remained unfermented after 48 hours. During fermentation, IGs released mono-, di-, or trisaccharides and lowered the pH of the broth, while producing short-chain fatty acids. The analysis also revealed that different IGs selectively promoted the proliferation of specific bacteria in the gut.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Yu Wu, Wengan Zhou, RuTing Feng, Duoduo Zhang, Yongkang Yu, Zuanyuan Huang, Junyi Yin, Xingtao Zhou, Shaoping Nie
Summary: The immunomodulatory effects of glucan from fruit body of Dictyophora rubrovolvata (DRP) were evaluated in this study. It was found that DRP can recover CTX-induced immunosuppression and activate dendritic cells (DCs) to increase cytokine secretion. The study also revealed that DRP regulates the AKT-STAT1 signaling pathway via Mincle in DCs to induce CD4(+) T cell proliferation and differentiation, thereby ameliorating CTX-induced immunosuppression.
Article
Food Science & Technology
Shanshan Zhang, Yonggan Sun, Qixing Nie, Jielun Hu, Wenwen Su, Zheyu Guo, Yanli Zhang, Shaoping Nie
Summary: Polysaccharides are commonly used as carriers for colon-targeted drugs, but their degradation and effects on gut microbiota in colitis conditions are unclear. In this study, the fermentation of four polysaccharides using colonic content microbiota from mice with colitis was performed. The results show that pectin and guar gum have a greater degree of degradation and promote the production of beneficial bacteria, while carrageenan and xanthan gum promote the proliferation of harmful bacteria.
Article
Chemistry, Multidisciplinary
Anqi Xie, Haihua Ji, Zheyi Liu, Yiqun Wan, Xuecong Zhang, Huihuang Xiong, Shao-Ping Nie, Hao Wan
Summary: Oral administration of probiotics is a promising method to alleviate inflammatory bowel diseases (IBDs), but sensitivity to the gastrointestinal environment and poor intestinal colonization hinder their effectiveness. In this study, a modified prebiotic-based shield (Fe-TA@mGN) was developed to enhance the stress resistance ability and intestinal colonization of Escherichia coli Nissle 1917 (EcN). The shield improved the survival rate of EcN and its retention in the intestine, leading to efficient alleviation of colitis. Additionally, the shield synergized with EcN to regulate gut microbiota and promote the production of short-chain fatty acids, further enhancing the alleviation of colitis.
Article
Chemistry, Applied
Siwen Lyu, Meiru Chen, Ying Wang, Deju Zhang, Songning Zhao, Jingbo Liu, Fengguang Pan, Ting Zhang
Summary: This study investigated the structural changes, physico-chemical properties and foaming ability of egg white protein hydrolysates with different hydrolysis times. The results showed that neutral protease-mediated hydrolysis for 15 minutes increased the foaming ability from 69.67% to 108.33%. The study also revealed that hydrolysis increased the proportion of polypeptides with small molecular weight and caused unfolding of the protein's structure, leading to increased adsorption to the air-water interface.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Hedi Wen, Deju Zhang, Zhenzhen Ning, Zihao Li, Yan Zhang, Jingbo Liu, Ting Zhang
Summary: This study investigated the effect of polyphenol structure on the foaming properties of protein by developing ovalbumin-polyphenol complexes. The results showed that the presence of fewer hydroxyl groups and ortho-aromatic structures in the polyphenols positively correlated with higher foaming properties of the complexes. Additionally, the addition of polyphenols promoted the adsorption and rearrangement of ovalbumin on the liquid-gas interface, thereby increasing its foaming properties.
FOOD HYDROCOLLOIDS
(2023)
Review
Agriculture, Multidisciplinary
Qi Yang, Siwen Lyu, Menglei Xu, Shengrao Li, Zhiyang Du, Xuanting Liu, Xiaomin Shang, Zhipeng Yu, Jingbo Liu, Ting Zhang
Summary: The study aims to review the effects of egg white protein (EWP) and its bioactive peptides on intestinal structural repair. The potential modulation mechanisms by which EWP and their peptides regulate the gut microbiota and intestinal barrier include restoring the structure of the intestinal barrier, enhancing the intestinal immune system, alleviating inflammation and oxidative damage, and increasing the abundance of beneficial bacteria and metabolites. Further research is needed to understand the coregulation of multiple signaling pathways and evaluate the combined effects of these mechanisms in experimental models. Human trials can provide new directions for development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Qi Yang, Xiaomin Shang, Ting Li, Siwen Lyu, Xuanting Liu, Zhiyang Du, Jingbo Liu, Ting Zhang
Summary: In this study, the effects of two different levels of Maillard reaction products (MRPs) of ovalbumin (OVA) on metabolites in colitis mice were explored using untargeted metabolomics techniques. It was found that MR could affect protein metabolites in vivo and MRPs of OVA could reduce the concentrations of IL-6 and IL-1β and improve intestinal permeability. Metabolomics results revealed that the degree of MR influenced the abundance of oligopeptides and bile acids in vivo. This study demonstrated that MRPs can regulate the abundance of metabolites such as taurocholic acid and putrescine, and repair the intestinal barrier in colitis mice through signaling pathways such as secondary bile acid biosynthesis, bile secretion, and ABC transporters.
Article
Biochemistry & Molecular Biology
Qianhuang Xiao, Wenqi Huang, Quanyong Wu, Hedi Xu, Yanli Zhang, Jingrui Yang, Shuigen Bian, Huizi Tan, Shaoping Nie
Summary: A sedentary lifestyle, unhealthy diet, and antibiotic use have been associated with an increased incidence of metabolic disorders and inflammation. This study focused on the effects of pectin, an edible polysaccharide, with different esterification degrees on mice treated with a high-fat diet and low-dose antibiotics. The results showed that different types of pectin had differential effects on metabolic health, inflammatory markers, gut microbiota, and serum metabolome.
Article
Agriculture, Multidisciplinary
Shi-Kang Chen, Yu-Hao Li, Xin Wang, Yu-Qing Guo, Xiao-Xiao Song, Shao-Ping Nie, Jun-Yi Yin
Summary: In this study, the structural order of Hericium erinaceus polysaccharides from different regions was evaluated using several analysis methods, revealing various structural types including fucogalactoglucan, glucofucogalactan, fucoglucan, and glucan. The main chains of heterogalactans consist of alpha-(1 -> 6)-Galp residues, which may be substituted by Fucp and Galp residues at O-2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
