Review
Biochemistry & Molecular Biology
Sylwia Szrok-Jurga, Aleksandra Czumaj, Jacek Turyn, Areta Hebanowska, Julian Swierczynski, Tomasz Sledzinski, Ewa Stelmanska
Summary: Fatty acid metabolism, especially beta-oxidation, plays a crucial role in human physiology and pathology. Beta-oxidation is not only involved in energy metabolism but also contributes to the synthesis of various important compounds. This review provides an overview of the importance of mitochondrial and peroxisomal beta-oxidation in different organs and cells and discusses the diseases associated with fatty acid oxidation disturbances. The review also highlights the abnormalities of fatty acid oxidation in cancer cells and diseases caused by mutations in enzymes involved in fatty acid oxidation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jing Quan, Can Cheng, Yue Tan, Nian Jiang, Chaoliang Liao, Weihua Liao, Ya Cao, Xiangjian Luo
Summary: Cancer cells frequently undergo metabolic reprogramming, and alterations in fatty acid metabolism have received increasing concerns. Recent research shows that ACSL3 plays a role in energy metabolic reprogramming and promotes metastasis in colorectal carcinoma cells through FAO pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yuxun Zhang, Eric Goetzman
Summary: Mitochondrial trifunctional protein (TFP) plays a crucial role in chain-shortening long-chain fatty acids within the mitochondria. Despite being heavily modified by acetyllysine and succinyllysine post-translational modifications, the functional significance of these modifications on TFP remains unclear. Research suggests that TFP in the membrane-bound state may be protected from lysine acylation, which could impact its enzymatic activity.
Article
Biochemistry & Molecular Biology
Huili Li, Jessica Seessle, Simone Staffer, Sabine Tuma-Kellner, Gernot Poschet, Thomas Herrmann, Walee Chamulitrat
Summary: Evidence suggests that a deficiency of FATP4 in liver cells can lead to a shift in TG metabolism, resulting in increased lipolysis and lipoprotein secretion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Plant Sciences
Huayan Zhao, Dylan K. Kosma, Shiyou Lu
Summary: Fatty acids play crucial roles in plants as components of lipid membranes, sources of energy, and signaling molecules. Changes in LACS activity can lead to pleiotropic phenotypes. This review provides a comprehensive analysis of LACS family enzymes and highlights areas for future research.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Food Science & Technology
Wenqi Shi, Jie Li, Yanfang Chen, Yefu Chen, Xuewu Guo, Dongguang Xiao
Summary: Metabolic engineering strategies were utilized to enhance the production of C6-C10 fatty acid ethyl esters in Saccharomyces cerevisiae, including improving precursor accumulation, increasing alcohol acyltransferase activity, and facilitating ester synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Karolis Petkevicius, Eleni Koutsoumpeli, Petri Christina Betsi, Bao-Jian Ding, Kanchana Rueksomtawin Kildegaard, Hilbert Jensen, Nora Mezo, Andrea Mazziotta, Anders Gabrielsson, Christina Sinkwitz, Bettina Lorantfy, Carina Holkenbrink, Christer Lofstedt, Dimitris Raptopoulos, Maria Konstantopoulou, Irina Borodina
Summary: This study developed a biotechnological method to produce the sex pheromone of the European corn borer using engineered yeast, achieving high titers of the pheromone component. The research opens up possibilities for fermentation-based production of ECB pheromones for pest management.
BIOTECHNOLOGY JOURNAL
(2021)
Review
Physiology
Kelsie R. S. Doering, Glafira Ermakova, Stefan Taubert
Summary: The genome of Caenorhabditis elegans contains 284 nuclear hormone receptor genes, with NHR-49 being one of the most well-studied. It is closely related to mammalian hepatocyte nuclear factor 4a and peroxisome proliferator-activated receptor a, and plays diverse roles in development, physiology, lipid metabolism, stress response, and innate immune response.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Horticulture
Yifei Wang, Jin Zhao, Hong Chen, Qing Zhang, Yusheng Zheng, Dongdong Li
Summary: In this study, a full-length cDNA encoding EgLACS9 was cloned from oil palm mesocarp and its subcellular localization in the endoplasmic reticulum (ER) was confirmed. Overexpression of EgLACS9 was shown to rescue LACS gene deficiency in S. cerevisiae and regulate fatty acid accumulation in oil palm, indicating its important role in lipid metabolic pathways.
SCIENTIA HORTICULTURAE
(2021)
Review
Biochemistry & Molecular Biology
Isha Kashyap, Rachayeeta Deb, Abhigna Battineni, Shirisha Nagotu
Summary: Beta-oxidation is an important metabolic pathway that is shared by mitochondria and peroxisomes in mammalian cells. In yeast, peroxisomes are the only site for fatty acid degradation. The enzyme acyl CoA oxidase, located in the peroxisomal matrix, catalyzes the first reaction of this pathway. Studies have shown the conserved function of this protein in fatty acid oxidation across different organisms. Its importance is demonstrated by the lethal conditions caused by altered function in humans. This review discusses various aspects, including gene expression, structure, folding, and import of the protein in both yeast and human cells, as well as recent findings on its role in human health, aging, and associated mutations in patients.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Virology
Laura A. St Clair, Stephanie A. Mills, Elena Lian, Paul S. Soma, Aritra Nag, Caroline Montgomery, Gabriela Ramirez, Nunya Chotiwan, Rebekah C. Gullberg, Rushika Perera
Summary: During infection with dengue viruses, the lipid landscape within host cells is altered to support viral replication and particle assembly. Fatty acyl-CoAs, which are key intermediates in lipid biosynthesis, play a role in this process. Loss of function of certain enzymes involved in fatty acyl-CoA metabolism leads to increased viral replication and infectious particle release, while loss of others results in decreased protein translation, genome replication, and virus release. The specific location of these enzymes within the cell may contribute to the differences observed in dengue virus infection. Loss of mitochondrial ACOT2 and ACOT7 expression also affects the expression of other ACOTs located in different organelles within the cell, suggesting a complex relationship between ACOTs in the virus lifecycle.
Article
Biochemistry & Molecular Biology
Johannes W. Kung, Anne-Katrin Meier, Max Willistein, Sina Weidenweber, Ulrike Demmer, Ulrich Ermler, Matthias Boll
Summary: This study presents the structure and function of an ACAD from anaerobic bacteria catalyzing a novel 1,4-elimination reaction. The unorthodox reactivity is explained by the presence of a catalytic aspartate base at C3 and by eliminating the catalytic glutamate base at C1. The results provide insights into the extended catalytic repertoire of ACADs and offer new biocatalytic options for the synthesis of cyclic 1,3-diene building blocks.
Article
Microbiology
Meixia He, Rui Guo, Gongshui Chen, Chao Xiong, Xiaoxia Yang, Yunlin Wei, Yuan Chen, Jingwen Qiu, Qi Zhang
Summary: Yeast cells have developed strategies to protect themselves from the negative impact of glucose starvation, such as producing more reactive oxygen species and inducing antioxidant systems. Transcriptome analysis revealed gene expression rearrangement and alterations in alternative carbon metabolic pathways under glucose starvation. Fatty acid beta-oxidation can increase the acetyl-CoA and carotenoid concentration.
Article
Biochemistry & Molecular Biology
Kento Otsuka, Megumi Sawai-Ogawa, Akio Kihara
Summary: Fatty acyl-CoA reductases (FARs) play a crucial role in the synthesis of meibum lipids, which are important for tear stability and eye health. This study reveals the specific functions of two FAR isozymes (FAR1 and FAR2) in the production of different chain lengths of fatty alcohols (FAls), and highlights the importance of FAl-containing meibum lipids in the formation of a functional tear film lipid layer.
Article
Cell Biology
Sembulingam Tamilzhalagan, Muthulakshmi Muthuswami, Jayaprakash Periasamy, Ming Hui Lee, Sun Young Rha, Patrick Tan, Kumaresan Ganesan
CELLULAR SIGNALLING
(2015)
Article
Biophysics
Kavitha Kandiah, Rajendran Venkatachalam, Chunyan Wang, Suresh Valiyaveettil, Kumaresan Ganesan
COLLOIDS AND SURFACES B-BIOINTERFACES
(2015)
Article
Chemistry, Multidisciplinary
Mathiyazhagan Arun Divakar, Velnathan Sudhamani, Sivakumar Shanmugam, Thillaichidambaram Muneeswaran, Sembulingam Tamilzhalagan, Muthiah Ramakritinan, Kumaresan Ganesan
Article
Oncology
Ramani Gopal, Usha Rani, Ram Murugesan, Kirushna Kumar, Ganesh Sanjeev, Kumaresan Ganesan
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2016)
Article
Genetics & Heredity
Vignesh Ramesh, Kumaresan Ganesan
Article
Cell Biology
Vignesh Ramesh, Kumaresan Ganesan
Article
Oncology
Vignesh Ramesh, Kumaresan Ganesan
INTERNATIONAL JOURNAL OF CANCER
(2016)
Article
Biochemistry & Molecular Biology
Sembulingam Tamilzhalagan, Muthulakshmi Muthuswami, Kumaresan Ganesan
MOLECULAR CARCINOGENESIS
(2017)
Article
Oncology
Vignesh Ramesh, Karthikeyan Selvarasu, Jaishree Pandian, Soundarajan Myilsamy, Chidambaranathan Shanmugasundaram, Kumaresan Ganesan
Article
Biochemistry & Molecular Biology
Tamilzhalagan Sembulingam, Dhanasekaran Rathinam, Kumaresan Ganesan
MOLECULAR CARCINOGENESIS
(2017)
Article
Chemistry, Multidisciplinary
Isaivani Dhinakaran, Vediappen Padmini, Kumaresan Ganesan, Karthikeyan Selvarasu
Article
Toxicology
Swathika Subburaman, Kumaresan Ganesan, Murugesan Ramachandran
JOURNAL OF ENVIRONMENTAL PATHOLOGY TOXICOLOGY AND ONCOLOGY
(2014)
Article
Multidisciplinary Sciences
Soundara Viveka Thangaraj, Vidyarani Shyamsundar, Arvind Krishnamurthy, Pratibha Ramani, Kumaresan Ganesan, Muthulakshmi Muthuswami, Vijayalakshmi Ramshankar
Article
Oncology
Kalaivani Kalamohan, Dhanasekaran Rathinam, Ponmathi Panneerpandian, Kumaresan Ganesan
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2017)