Review
Chemistry, Multidisciplinary
Yi Xie, Huimin Li, Fumin Chen, Srisruthi Udayakumar, Khyati Arora, Hui Chen, Yang Lan, Qinqin Hu, Xiaonong Zhou, Xiaokui Guo, Leshan Xiu, Kun Yin
Summary: Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. This review highlights the recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis, including detection mechanisms, microfluidic devices, and improvement strategies.
Review
Biotechnology & Applied Microbiology
Tien Van Vu, Duong Thi Hai Doan, Jihae Kim, Yeon Woo Sung, Mil Thi Tran, Young Jong Song, Swati Das, Jae-Yean Kim
Summary: The ultimate goals of genetic engineering are to achieve gene editing and allele introgression with absolute precision and control. Various approaches have been developed in plants for precision genome editing, with significant advancements in HR-mediated editing frequencies through CRISPR/Cas technology. Despite this progress, further research is needed for commercially viable applications of precise genome editing, potentially requiring alternative innovative methods.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Behavioral Sciences
Arshad Mehmood, Wajid Ali, Zaheer Ud Din, Shuang Song, Muhammad Sohail, Wahid Shah, Jiangyuan Guo, Ruo-Yi Guo, Ikram Ilahi, Suleman Shah, Fadhl Al-Shaebi, Liaqat Zeb, Ernest Amponsah Asiamah, Zaid Al-Dhamin, Hazrat Bilal, Bin Li
Summary: Genome-editing technology such as CRISPR/Cas9 has bridged the translational gap in gene therapy treatments, particularly in the experimental and therapeutic applications for central nervous system diseases. Studies have identified genetic mutations and duplications related to Parkinson's disease pathogenesis, suggesting that gene editing through CRISPR technology could be beneficial for PD treatment. This highlights the importance and potential of CRISPR technology in research.
BRAIN AND BEHAVIOR
(2021)
Article
Genetics & Heredity
Hua Jiang, Kun Duan, Xu Han, Jun Wang, Xiao Liu, Maoxiao Yan, Yunxiu Wang, Hongyan Liu, Huiling Shi, Xiaoqing Gao, Chuan Ouyang, Xue Fu, Xinxin Zhang, Chao Liu
Summary: In this study, Cas13a was utilized for the detection of genetic mutations by isothermally amplifying mitochondrial point mutations from human genomic DNA. The approach involved the use of transcription-mediated amplification to generate CRISPR RNA, demonstrating the potential for rapid mutation detection within 90 minutes. Various designs of CRISPR DNA were explored to enhance assay specificity, with the possibility of a one-step single-tube reaction for mutation detection and the potential for automated applications in screening mitochondrial diseases.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong, Xiaoming Zhou
Summary: CRISPR-based diagnoses, derived from gene-editing technology, have unique features such as recognition at physiological temperature, excellent specificity, and high-efficiency signal amplification capabilities. Micro/nanotechnologies play key roles in the advancement of CRISPR diagnosis, including the use of nanoprobes, nanochips, microfluidic technology, and lateral flow strips, leading to new opportunities and improvements such as equipment-free detection, more compact detection systems, improved sensitivity, and quantitative capabilities. Despite significant progress, CRISPR diagnosis has not yet reached its full potential, with upcoming opportunities and improvements expected to be driven by micro/nanotechnologies.
Article
Biochemistry & Molecular Biology
Jen-Hui Tsou, Hongjie Liu, Sanford A. Stass, Feng Jiang
Summary: This study developed a rapid CRISPR test for sensitive detection of SARS-CoV-2 without the need for RNA extraction, allowing for completion within one hour. The test is simple to operate, requiring minimal equipment, and results can be visualized immediately using a UV light illuminator or paper strips.
Review
Plant Sciences
Yuchun Rao, Xi Yang, Chenyang Pan, Chun Wang, Kejian Wang
Summary: CRISPR-Cas9 is a promising gene editing technology with the advantages of simplicity, high efficiency, high specificity, and low cost. This review systematically summarizes its generation, advantages, and three derivative technologies, as well as its applications in crop improvement.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemical Research Methods
Sara Selma, Evi Ceulemans, Alain Goossens, Elia Lacchini
Summary: The plant kingdom is a major source of feedstock, food, and added-value compounds. Engineering plant metabolic pathways is challenging due to complex interaction networks involving multiple genes, enzymatic steps, and metabolites. However, the development of CRISPR-Cas technologies has provided efficient tools for multigene editing in plants, enabling improvements in agronomic and metabolic traits. This article provides an overview of the achievements and perspectives of CRISPR technology in plant metabolic engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Cheng Yang, Wangfeng Shao, Lingling Wei, Lingxiao Chen, Aihua Zhu, Zhiming Pan
Summary: Salmonella as a zoonotic pathogen, especially in transmission between household pets and humans, has attracted worldwide attention. A study conducted in Xuzhou, Jiangsu Province, China, investigated the distribution of dog Salmonella from pet hospitals and breeding base. The results showed that S. Kentucky was the most prevalent serotype, and CRISPR genes have better recognition ability for the same serotype.
Article
Chemistry, Multidisciplinary
Cheng-Yu Li, Bei Zheng, Jiang-Tao Li, Jia-Ling Gao, Yu-Heng Liu, Dai-Wen Pang, Hong-Wu Tang
Summary: The CRISPR/Cas12a system offers an alternative opportunity for designing fluorescence biosensors with outstanding precision in target recognition. By utilizing collateral cleavage and representative transduction pathways, the system can extend sensing coverage and improve assay sensitivity. The developed biosensors not only exhibit ultrasensitive behavior towards non-nucleic acid analytes but also show practical application capacity in real samples.
Review
Chemistry, Multidisciplinary
Mengdi Bao, Qun Chen, Zhiheng Xu, Erik C. Jensen, Changyue Liu, Jacob T. Waitkus, Xi Yuan, Qian He, Peiwu Qin, Ke Du
Summary: CRISPR, with its unique properties, has emerged as a powerful molecular biosensing tool for nucleic acids and biomarkers. However, several key challenges need to be addressed for it to become a new gold standard. This paper reviews the history of biosensors, discusses current challenges and recent breakthroughs in CRISPR-based nucleic acid detection, and focuses on future advancements needed for CRISPR-based molecular biosensors.
Article
Veterinary Sciences
Hung-Yueh Yeh, Amal Awad, Michael J. Rothrock
Summary: The study explored the diversity of CRISPR sequences in C. jejuni isolates from an animal farm, revealing various combinations of CRISPR sequence lengths and spacer numbers. The results indicated that CRISPR genotyping combined with MLST methods could discriminate C. jejuni isolates, highlighting the need for further research on the application of CRISPR in outbreaks.
VETERINARY MEDICINE AND SCIENCE
(2021)
Review
Agricultural Engineering
Tse-Min Lee, Jia-Yi Lin, Tsung-Han Tsai, Ru-Yin Yang, I-Son Ng
Summary: Carbon dioxide is the main greenhouse gas and a critical issue for global warming and climate change. Microalgae, which are inconspicuous microorganisms, account for 40% of carbon fixation in photosynthetic plants and have higher photosynthetic efficiency. They convert carbon into various useful compounds. Genetic engineering and metabolic engineering are used to increase the growth rate and biomass of microalgae to achieve carbon neutrality. CRISPR/Cas9 is an efficient tool to enhance productivity in microalgae by regulating multiple genes simultaneously. This review summarizes the technologies, bioinformatics tools, and systematic engineering approaches for carbon neutrality and circular economy, bringing us closer to a decarbonized society.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Sheng Ding, Yinghua Wei, Gangyi Chen, Feng Du, Xin Cui, Xin Huang, Yi Yuan, Juan Dong, Zhuo Tang
Summary: We report a CRISPR/Cas12a signal expansion platform for rapid and sensitive detection of FEN1. This method utilizes transcription and enzyme activity to detect FEN1 activity. It has high specificity and a low limit of detection, and shows potential in inhibitor screening.
ANALYTICAL CHEMISTRY
(2022)
Article
Agricultural Engineering
Min Seo Jeon, Sang-Il Han, Minsol Jeon, Yoon-E Choi
Summary: Genetic manipulation of Porphyridium sp. increased the production of phycoerythrin via a CRISPR/Cas9 RNP delivery system targeting the Chs1 gene. Mutants showed enhanced phycoerythrin contents without affecting growth or polysaccharide production, indicating a potential for commercial production if Porphyridium is chosen as the source.
BIORESOURCE TECHNOLOGY
(2021)
Article
Oncology
Jue Jiang, Jingchao Wang, Ming Yue, Xiaolian Cai, Tianci Wang, Chao Wu, Hexiu Su, Yanwu Wang, Meng Han, Yingchi Zhang, Xiaofan Zhu, Peng Jiang, Peng Li, Yonghua Sun, Wuhan Xiao, Hui Feng, Guoliang Qing, Hudan Liu
Article
Biochemistry & Molecular Biology
Yonghua Sun, Bo Zhang, Lingfei Luo, De-Li Shi, Han Wang, Zongbin Cui, Honghui Huang, Ying Cao, Xiaodong Shu, Wenqing Zhang, Jianfeng Zhou, Yun Li, Jiulin Du, Qingshun Zhao, Jun Chen, Hanbing Zhong, Tao P. Zhong, Li Li, Jing-Wei Xiong, Jinrong Peng, Wuhan Xiao, Jian Zhang, Jihua Yao, Zhan Yin, Xianming Mo, Gang Peng, Jun Zhu, Yan Chen, Yong Zhou, Dong Liu, Weijun Pan, Yiyue Zhang, Hua Ruan, Feng Liu, Zuoyan Zhu, Anming Meng, Yonghua Sun, Luyuan Pan, Houpeng Wang, Weixun Xie, Mudan He, Ding Ye, Kuoyu Li, Feng Xiong, Liyue Liu, Linglu Li, Yun Zhang, Bo Zhang, Da Liu, Zhenchao Cheng, Yingying Hu, Qian Wu, Zhou Luo, Yutian Zhang, Yingdan Wu, Wenyuan Li, Lingfei Luo, Jianlong Ma, Dashuang Mo, Pengcheng Cai, Jinzi Chen, Junhui Sun, Yang Zhou, Chuan Wu, Rui Ni, De-Li Shi, Ming Shao, Yan-Jun Zhang, Li-Jun Feng, Xiao-Ning Cheng, Ji-Tong Li, Yan-Yi Xing, Yan Zhang, Ya-Ping Meng, Bei-Bei Yu, Han Wang, Jian Huang, Shuqing Zhang, Cheng Ji, Yicheng Tan, Jingjing Wang, Fanmiao Zhang, Mingyong Wang, Guodong Huang, Zhaomin Zhong, Wei Zhang, Zongbin Cui, Yong Long, Guili Song, Xiaohui Li, Xixi Li, Kai Chen, Jing Ren, Junjun Yan, Qing Li, Honghui Huang, Hua Ruan, Shicheng Zhu, Xuejiao Chen, Jingjing Pan, Faming Jiang, Jiehui Chen, Chao Huang, Guoping Dong, Xiaogui Yi, Ying Cao, Ruikun Hu, Weilai Huang, Jiangfang Liu, Hong WangXiao, Dong Shu, Jianhong Xia, Shaoyang Zhao, Pengtao Wang, Xiuhua Wu, Wenqing Zhang, Yiyue Zhang, Mengchang Xu, Wei Liu, Zhibing Huang, Qing Lin, Mei Wu, Jianfeng Zhou, Aibo Sheng, Peipei Tang, Xiaoxia Gong, Wei Mou, Congcong Zu, Yun Li, Ling Lu, Yunzhang Liu, Xiaozhi Rong, Jianyang Chen, Jiulin Du, Jiwen Bu, Xufei Du, Tingting Liu, Shanye Gu, Qingshun Zhao, Zhangji Dong, Xiaohua Dong, Shasha Cao, Yunyun Yue, Chun Gu, Meijing Liu, Jun Chen, Hongjian Gong, Hanbing Zhong, Xuanjun Yang, Jiahao Chen, Qiushi Xu, Tao P. Zhong, Daqin Jin, Peilu She, Jianjian Sun, Kaa Seng Lai, Li Li, Yu Zhang, Yanyan Shi, Fangying Zhao, Jing-Wei Xiong, Meijun Pang, Lu Gao, Lei Lei, Jinrong Peng, Jane Lo, Wuhan Xiao, Xiaolian Cai, Gang Ouyang, Jian Zhang, Weirui Ma, Liyun Miao, Jihua Yao, Yu Hu, Zhan Yin, Xianming Mo, Xue Li, Gang Peng, Cuizhen Zhang, Fenghua Wang, Jun Zhu, Ruimeng Yang, Hao Yuan, Yan Chen, Yi Pan, Yong Zhou, Dong Liu, Feng Zhao, Weijun Pan, Feng Liu, Yanyan Ding, Yuanyuan Xue, Lu Wang, Yifan Zhang, Dongyuan Ma, Xinyan Lu, Shuai Gao, Jun Xia, Zuoyan Zhu, Anming Meng, Xingfeng Liu, Juhui Qiu, Bo Gong, Luxi Chen, Cong Xiong, Likun Yao, Cencan Xing, Yixia Wang, Weimin Shen, Jiawei Sun
Article
Endocrinology & Metabolism
Qifeng Zhang, Ding Ye, Houpeng Wang, Yaqing Wang, Wei Hu, Yonghua Sun
Article
Fisheries
Yousef Mohammed, Ding Ye, Mudan He, Houpeng Wang, Yanhua Li, Danxiang Han, Zuoyan Zhu, Yonghua Sun
Summary: The study successfully engineered fish cells and zebrafish embryos to synthesize ketocarotenoids, with significantly increased content in fish models both in vitro and in vivo, demonstrating the potential for animals to manufacture these valuable molecules.
Article
Endocrinology & Metabolism
Yaqing Wang, Ding Ye, Fenghua Zhang, Ru Zhang, Junwen Zhu, Houpeng Wang, Mudan He, Yonghua Sun
Summary: This study demonstrates the functional importance of cyp11a2 in early oogenesis and differentiation of SSCs in zebrafish. Depletion of cyp11a2 results in steroidogenic deficiencies, sex reversal, and infertility in males. The mutation leads to apoptosis of oocytes in developing ovaries and dysfunction of Sertoli cells and Leydig cells in developing testes.
Article
Biology
Fenghua Zhang, Yongkang Hao, Xianmei Li, Yi Li, Ding Ye, Ru Zhang, Xiaosi Wang, Mudan He, Houpeng Wang, Zuoyan Zhu, Yonghua Sun
Summary: With an optimized SSCT procedure, researchers successfully obtained genome-edited sperm derived from Chinese rare minnow in zebrafish, demonstrating the feasibility of surrogate production of gametes from a different subfamily using surrogate reproduction techniques. Knockout of pou5f3 and chd genes in Gr SSCs also led to the successful production of genome-edited sperm. This study opens up new possibilities for the future breeding of commercial fish and livestock.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Binbin Tao, Hongling Hu, Ji Chen, Lu Chen, Daji Luo, Yonghua Sun, Feng Ge, Zuoyan Zhu, Vance L. Trudeau, Wei Hu
Summary: Recent research has identified a new subunit of the SIN3A-HDAC complex, called Sinhcaf, that plays a crucial role in germ plasm aggregation and primordial germ cell (PGC) specification. Sinhcaf-dependent histone deacetylation is essential for proper PGC development, as mutations in sinhcaf result in defects in germ plasm aggregation and decreased PGC abundance. Sinhcaf achieves this by regulating the acetylation status of the kif26ab promoter, which affects its transcription and ultimately PGC specification.
Article
Genetics & Heredity
Yan Zhao, L. Filipe C. Castro, Oscar Monroig, Xiaojuan Cao, Yonghua Sun, Jian Gao
Summary: This study generated a zebrafish mutant lacking ppar γ with CRISPR/Cas9 technology and investigated its regulatory network. The deletion of ppar γ protected against high-fat diet-induced lipid deposition and involved protein kinases related to lipid metabolism.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2022)
Article
Cell Biology
Haibo Xie, Xiaosi Wang, Minjun Jin, Lanqin Li, Junwen Zhu, Yunsi Kang, Zhe Chen, Yonghua Sun, Chengtian Zhao
Summary: Meiosis is crucial for evolution and genetic diversity, and cilia play a critical role in the meiotic recombination process. Our study reveals the involvement of cilia in meiosis and suggests that extracellular signals may regulate meiotic recombination through this organelle.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yao Xiao, Shengbo Jiao, Miao He, Da Lin, Huanyan Zuo, Jiahao Han, Yonghua Sun, Gang Cao, Zhi Chen, Huan Liu
Summary: This study utilizes an integrated epigenomic dataset to identify functional variants associated with orofacial clefts, providing a framework for risk assessment and understanding of clefts.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ru Zhang, Yi-Xuan Tu, Ding Ye, Zhenglong Gu, Zhen-Xia Chen, Yonghua Sun
Summary: This study identifies zebrafish pld6 as a novel germline-specific gene that plays a crucial role in the maintenance and differentiation of germline stem and progenitor cells (GSPCs). Pld6 knockout mutants develop exclusively into infertile males, as the GSPCs fail to differentiate and undergo apoptosis. Furthermore, pld6-depleted GSPCs display impaired mitochondrial fusion, defects in piRNA biogenesis, and transposon suppression.
Article
Developmental Biology
Mudan He, Shengbo Jiao, Ru Zhang, Ding Ye, Houpeng Wang, Yonghua Sun
Summary: This study demonstrates that maternal Nanog regulates oogenesis and early embryogenesis through translational control of maternal mRNA via a mechanism whereby Nanog acts as a transcriptional repressor to suppress transcription of eef1a1l2. The loss of Nanog leads to defects in egg maturation and increased translational activity, which can be rescued by depleting eef1a1l2.
Article
Zoology
Shan-Shan Xu, Yi Li, Hou-Peng Wang, Wen-Bo Chen, Ya-Qing Wang, Zi-Wei Song, Hui Liu, Shan Zhong, Yong-Hua Sun
Summary: This study investigates the role of Stearyl coenzyme A desaturase (SCD) in lipid metabolism and sexual development using a zebrafish scd mutant. The mutant fish displayed increased levels of saturated fatty acids and decreased levels of monounsaturated fatty acids. They also exhibited abnormalities in body size, fat accumulation in the liver, hepatic mitochondrial dysfunction, and cell apoptosis. The mutant males showed defective mating behavior due to genital papillae defects but had functional mature sperm. Overexpression of scd rescued all the observed defects in the mutant fish.
ZOOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Zhe Zhang, Chun Yang, Zixu Wang, Liwei Guo, Yongpan Xu, Ce Gao, Yonghua Sun, Zhenhai Zhang, Jinrong Peng, Minjie Hu, Li Jan Lo, Zhipeng Ma, Jun Chen
Summary: Food digestion requires coordinated cooperation of different digestive organs. In this study, we demonstrated that Wdr5-mediated H3K4 tri-methylation plays a critical role in regulating cell differentiation, proliferation, and apoptosis during zebrafish organogenesis of three major digestive organs. Our findings provide insights into the common molecular mechanisms of cell fate determination in different digestive organs and shed light on the mechanistic basis of human diseases in these organs.
CELL DEATH DISCOVERY
(2023)
Article
Biotechnology & Applied Microbiology
Yousef Mohammed, Ding Ye, Mudan He, Houpeng Wang, Zuoyan Zhu, Yonghua Sun
Summary: This study successfully engineered animal cells to biosynthesize astaxanthin, with significant implications for transgenic animals and the aquaculture sector. After optimization, the concentration of astaxanthin increased significantly, making it a promising application.
BIOENGINEERING-BASEL
(2023)