Article
Cell Biology
Rui Wu, Junfeng Zhan, Bo Zheng, Zhen Chen, Jianbo Li, Changrong Li, Rong Liu, Xinhua Zhang, Xiaoyan Huang, Mengcheng Luo
Summary: The study reveals that SYMPK plays a critical role in male germ cells development, and its knockout leads to male infertility in mice. Deletion of SYMPK affects spermatogenesis and meiotic progression by influencing alternative splicing and gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Xiaohong Yao, Chaofan Wang, Longjie Sun, Lu Yan, Xuexue Chen, Zheng Lv, Xiaomei Xie, Shuang Tian, Wenbo Liu, Lei Li, Hua Zhang, Jiali Liu
Summary: In this study, it was discovered that BCAS2 protein regulates pre-mRNA splicing in granulosa cells, affecting their proliferation and survival. BCAS2 participates in the PRP19 complex to mediate alternative splicing of E2f3 and Flt3l mRNA, thereby inhibiting the cell cycle. These findings reveal the function of BCAS2 in granulosa cells.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jiaqi Zhang, Wenbo Liu, Guangyue Li, Chengpeng Xu, Xiaoqing Nie, Dandan Qin, Qizhi Wang, Xukun Lu, Jianqiao Liu, Lei Li
Summary: Alternative splicing is an important mechanism for regulating organogenesis and fertility. BCAS2 plays a crucial role in mouse oocytes, participating in oocyte development through regulating splicing and influencing follicular development.
Article
Veterinary Sciences
Junfeng Zhan, Jianbo Li, Yuerong Wu, Panfeng Wu, Ziqi Yu, Peng Cui, Mofan Zhou, Yumin Xu, Tingyu Jin, Ziye Du, Mengcheng Luo, Cong Liu
Summary: Sugp2 is a chromatin-associated candidate protein that plays a role in spermatogenesis. Depletion of Sugp2 by CRISPER-Cas9 technology revealed that it controls a network of genes related to metal ion and ATP binding.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kazashi Kato, Rina Udagawa, Yuki Hayashi, Masayoshi Maki, Makiko Yanagida, Sae Higashiura, Reina Yagishita, Haruka Shimamoto, Keiji Kimura
Summary: This study demonstrates the mitotic functions of SF3B14 in controlling mitotic chromosome behavior by regulating the pre-mRNA splicing of TUBGCP6. SF3B14 depletion leads to prolonged mitosis and various mitotic defects, suggesting its crucial role in mitotic processes. Although predominantly localized in the nucleoplasm during interphase, SF3B14 does not colocalize with specific mitotic structures during mitosis, indicating a potential indirect regulation mechanism.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Cell Biology
Saad Aldalaqan, Caroline Dalgliesh, Sara Luzzi, Chileleko Siachisumo, Louise N. Reynard, Ingrid Ehrmann, David J. Elliott
Summary: Recent findings highlight the essential role of RNA binding protein RBMXL2 in male meiosis, acting as a guardian of the transcriptome to prevent aberrant splice sites and maintain gene expression. This protective mechanism in meiosis may have evolved to ensure sperm production and male fertility, with implications for understanding neurodegenerative diseases like ALS and Alzheimer's.
Article
Biochemistry & Molecular Biology
Mingtian Deng, Baobao Chen, Zifei Liu, Yongjie Wan, Dongxu Li, Yingnan Yang, Feng Wang
Summary: YBX1 plays important roles in maternal mRNA decay, alternative splicing, and transcriptional activity during early embryogenesis. These findings broaden our understanding of YBX1 functions.
CELL AND BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
James D. D. Fackenthal
Summary: Cancer is a leading cause of death worldwide. Alternative mRNA splicing has recently been recognized as a major contributor to cancer initiation and progression. Cancer cells exhibit different distribution of splicing variants compared to non-cancer cells, and they are more sensitive to drugs targeting the splicing regulatory network. Certain recurring splicing variants have been shown to contribute to tumor progression, while some disruption events result from mutations in splicing regulatory sequences or shifts in balance among naturally occurring splicing variants of mRNAs involved in cell cycle progression and apoptosis regulation. This review explores cancer-related alternative splicing events and potential therapies.
Article
Cell Biology
Yanfei Wang, Cuiqiao Zhang, Wu Peng, Haibo Du, Yuehui Xi, Zhigang Xu
Summary: This study further explored the role of RBM24 in hearing and balance through conditional knockout mice and found that the loss of RBM24 results in severe deficits. It was observed that the development of hair cells was significantly affected, leading to degeneration and disorganization of the hair bundles. RNAseq analysis also identified several genes whose mRNA stability or pre-mRNA alternative splicing is affected by RBM24 knockout.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Weiwei Liang, Fangfang Sun
Summary: This study identified 1005 survival-associated alternative splicing events in ACC patients and constructed a prediction model for prognostic genes, providing biomarkers for disease progression and potentially rich source of novel therapeutic targets. A nomogram integrating clinical-pathological factors and riskscore was developed for predicting 1- and 3-year survival rate.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Oncology
Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee K. Kim
Summary: Alternative pre-mRNA splicing plays a critical role in generating multiple mRNA and increasing protein diversity. Specific splicing isoforms have been found to be important in cellular processes, particularly in regulating cell numbers. Abnormal expression of splicing isoforms and factors, as well as disruptions in splicing caused by genetic mutations, are implicated in cancer development and progression. Alternative splicing has potential as a therapeutic target for cancer.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Zhenyu Ma, Mingyue Li, Huaning Zhang, Baihui Zhao, Zihui Liu, Shuonan Duan, Xiangzhao Meng, Guoliang Li, Xiulin Guo
Summary: High temperature reduces crop production, especially wheat. A novel splice variant, TaHsfA2-7-AS, was induced by heat and positively regulated thermotolerance in wheat. TaHsfA2-7-AS encodes a truncated isoform and its expression is up-regulated during heat shock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick, P. Hande Ozdinler, Douglas A. Gray, Kelsey Fisher-Wellman, Brian P. Hermann, Christopher B. Geyer
Summary: We have developed a facile in vitro differentiation and meiotic initiation system that can be manipulated using chemical agents that cannot be administered to live animals. Additionally, we introduced a transgenic mouse model enabling the isolation of specific developmental stages of spermatogonia and meiotic spermatocytes.
Article
Immunology
Elena Putscher, Michael Hecker, Brit Fitzner, Nina Boxberger, Margit Schwartz, Dirk Koczan, Peter Lorenz, Uwe Klaus Zettl
Summary: This study found that genetic variants from MS risk loci affect pre-mRNA splicing. These findings substantiate the role of alternative splicing events in the genetics of MS. Further research on how disease-causing genetic variants modify the interactions between splicing regulatory sequence elements and RNA-binding proteins can help deepen our understanding of the genetic susceptibility to MS.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaohong Yao, Chaofan Wang, Weiran Yu, Longjie Sun, Zheng Lv, Xiaomei Xie, Shuang Tian, Lu Yan, Hua Zhang, Jiali Liu
Summary: This study found that SRSF1 plays an important role in mouse granulosa cells. Specific knockout of SRSF1 leads to inhibition of follicular development, decreased granulosa cell proliferation, and increased apoptosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Genetics & Heredity
Yunlong Xiang, Yu Zhang, Qianhua Xu, Chen Zhou, Bofeng Liu, Zhenhai Du, Ke Zhang, Bingjie Zhang, Xiaoxiao Wang, Srimonta Gayen, Ling Liu, Yao Wang, Yuanyuan Li, Qiujun Wang, Sundeep Kalantry, Lei Li, Wei Xie
Article
Multidisciplinary Sciences
Xiaohua Lei, Yujing Cao, Baohua Ma, Yunfang Zhang, Lina Ning, Jingjing Qian, Liwen Zhang, Yongcun Qu, Tao Zhang, Dehong Li, Qi Chen, Junchao Shi, Xudong Zhang, Chiyuan Ma, Ying Zhang, Enkui Duan
NATIONAL SCIENCE REVIEW
(2020)
Article
Genetics & Heredity
Jun Wang, Xueying Xie, Junchao Shi, Wenjun He, Qi Chen, Liang Chen, Wanjun Gu, Tong Zhou
GENOMICS PROTEOMICS & BIOINFORMATICS
(2020)
Article
Cell Biology
Xiaoxiao Wang, Yunlong Xiang, Yang Yu, Ran Wang, Yu Zhang, Qianhua Xu, Hao Sun, Zhen-Ao Zhao, Xiangxiang Jiang, Xiaoqing Wang, Xukun Lu, Dandan Qin, Yujun Quan, Jiaqi Zhang, Ng Shyh-Chang, Hongmei Wang, Naihe Jing, Wei Xie, Lei Li
Summary: This study reported the establishment of self-renewing formative pluripotent stem cells (fPSCs) that exhibit features of epiblast cells poised for gastrulation, and can be derived from different cell sources. fPSCs show distinct transcriptomic features of formative pluripotency compared to naive ESCs and primed EpiSCs, and have unique epigenetic states similar to E6.5 epiblast cells.
Editorial Material
Cell & Tissue Engineering
Ng Shyh-Chang, Lei Li
Summary: Two papers in this issue of Cell Stem Cell demonstrate that polarized epithelial and germcell-competent formative PSCs can be maintained under modified activin-dependent conditions. These findings challenge previous debates on stabilizing "formative" pluripotency in PSC lines.
Letter
Cell Biology
Yongcun Qu, Qi Chen, Shanshan Guo, Chiyuan Ma, Yonggang Lu, Junchao Shi, Shichao Liu, Tong Zhou, Taichi Noda, Jingjing Qian, Liwen Zhang, Xili Zhu, Xiaohua Lei, Yujing Cao, Wei Li, Nicolas Plachta, Martin M. Matzuk, Masahito Ikawa, Enkui Duan, Ying Zhang, Hongmei Wang
Article
Cell Biology
Junchao Shi, Yunfang Zhang, Dongmei Tan, Xudong Zhang, Menghong Yan, Ying Zhang, Reuben Franklin, Marta Shahbazi, Kirsty Mackinlay, Shichao Liu, Bernhard Kuhle, Emma R. James, Liwen Zhang, Yongcun Qu, Qiwei Zhai, Wenxin Zhao, Linlin Zhao, Changcheng Zhou, Weifeng Gu, Jernej Murn, Jingtao Guo, Douglas T. Carrell, Yinsheng Wang, Xuemei Chen, Bradley R. Cairns, Xiang-lei Yang, Paul Schimmel, Magdalena Zernicka-Goetz, Sihem Cheloufi, Ying Zhang, Tong Zhou, Qi Chen
Summary: Shi et al. profiled small non-coding RNAs (sncRNAs) through PANDORA-seq, identifying tissue-specific transfer RNA- and ribosomal RNA-derived small RNAs, as well as sncRNAs with dynamic changes during induced pluripotent stem cell reprogramming.
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jennifer Cable, Edith Heard, Tetsuro Hirose, Kannanganattu Prasanth, Ling-Ling Chen, Jonathan E. Henninger, Sofia A. Quinodoz, David L. Spector, Sarah D. Diermeier, Allison M. Porman, Dhiraj Kumar, Mark W. Feinberg, Xiaohua Shen, Juan Pablo Unfried, Rory Johnson, Chun-Kan Chen, Jeremy E. Wilusz, Adelheid Lempradl, Sean E. McGeary, Lamia Wahba, Anna Marie Pyle, Amanda E. Hargrove, Matthew D. Simon, Marco Marcia, Roza K. Przanowska, Howard Y. Chang, Samie R. Jaffrey, Lydia M. Contreras, Qi Chen, Junchao Shi, Joshua T. Mendell, Lin He, Erwei Song, John L. Rinn, Mukesh Kumar Lalwani, Murat Can Kalem, Edward B. Chuong, Lynne E. Maquat, Xuhang Liu
Summary: The human transcriptome contains a variety of noncoding RNAs that play important roles in transcriptional regulation, epigenetic modifications, translation, and cell signaling. Research on noncoding RNAs in disease mechanisms and as potential drug targets is ongoing. The Keystone eSymposium on Noncoding RNAs aimed to accelerate the understanding of RNA basic biology and facilitate the translation of findings into clinical applications by bringing together researchers in RNA biology, structure, and technologies.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jiaqi Zhang, Wenbo Liu, Guangyue Li, Chengpeng Xu, Xiaoqing Nie, Dandan Qin, Qizhi Wang, Xukun Lu, Jianqiao Liu, Lei Li
Summary: Alternative splicing is an important mechanism for regulating organogenesis and fertility. BCAS2 plays a crucial role in mouse oocytes, participating in oocyte development through regulating splicing and influencing follicular development.
Review
Biochemistry & Molecular Biology
Qi Chen, Xudong Zhang, Junchao Shi, Menghong Yan, Tong Zhou
Summary: tsRNA, derived from tRNA cleavage, are ancient small RNAs with versatile roles in fundamental biological processes. These roles stem from sequence conservation, RNA modifications, and protein-binding abilities, suggesting the need for expanding tsRNA research into broader areas for precision medicine.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Cell Biology
Junchao Shi, Tong Zhou, Qi Chen
Summary: This article discusses recent approaches for discovering sncRNAs, limitations associated with sncRNA expression analyses, and emerging methods for direct and simultaneous detection of multiple RNA modifications.
NATURE CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yujun Quan, Meijiao Wang, Chengpeng Xu, Xiaoxiao Wang, Yu Wu, Dandan Qin, Yuxuan Lin, Xukun Lu, Falong Lu, Lei Li
Summary: Mammalian early epiblasts undergo pluripotency transitions from naive to formative states, involving extensive transcriptome changes. The deadenylase Cnot8 of Ccr4-Not complex is found to play crucial roles in this transition. Knock out of Cnot8 leads to embryonic lethality in mice, but Cnot8 knockout embryonic stem cells (ESCs) can be established. Compared to normal ESCs, Cnot8 knockout cells exhibit highly expressed genes during their differentiation into the formative state.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Wanjun Gu, Junchao Shi, Hui Liu, Xudong Zhang, Jin J. Zhou, Musheng Li, Dandan Zhou, Rui Li, Jingzhu Lv, Guoxia Wen, Shanshan Zhu, Ting Qi, Wei Li, Xiaojing Wang, Zhaohua Wang, Hua Zhu, Changcheng Zhou, Kenneth S. Knox, Ting Wang, Qi Chen, Zhongqing Qian, Tong Zhou
Article
Neurosciences
Xudong Zhang, Fatima Trebak, Lucas A. C. Souza, Junchao Shi, Tong Zhou, Patrick G. Kehoe, Qi Chen, Yumei Feng Earley
NEUROBIOLOGY OF DISEASE
(2020)
Article
Medicine, Research & Experimental
Shumei Miao, Dandan Zhao, Xiaoxiao Wang, Xuan Ni, Xing Fang, Miao Yu, Lingqun Ye, Jingsi Yang, Hongchun Wu, Xinglong Han, Lina Qu, Lei Li, Feng Lan, Zhenya Shen, Wei Lei, Zhen-Ao Zhao, Shijun Hu
