Article
Biochemistry & Molecular Biology
Takaaki Hashimoto, Takaaki Sugihara, Tsutomu Kanda, Tomoaki Takata, Hajime Isomoto
Summary: The study demonstrates the therapeutic potential of 5-ALA in treating hepatic steatosis by alleviating ER stress and lipoapoptosis through HO-1 induction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Feng Wang, Lili Han
Summary: SGK1 alleviates doxorubicin-induced cardiotoxicity and cardiomyopathy by repressing endoplasmic reticulum stress (ERS).
Article
Multidisciplinary Sciences
Qi Yang, Anju Kelkar, Anirudh Sriram, Ryoma Hombu, Thomas A. Hughes, Sriram Neelamegham
Summary: N-linked glycans on the SARS-CoV-2 Spike protein play a critical role in viral function and infectivity, making them potential drug targets for COVID-19.
Article
Cell Biology
Ruiyuan Xu, Jianlu Song, Rexiati Ruze, Yuan Chen, Xinpeng Yin, Chengcheng Wang, Yupei Zhao
Summary: This study reveals squalene epoxidase (SQLE) as a novel oncogene that promotes pancreatic cancer (PC) growth by mitigating endoplasmic reticulum stress and activating lipid raft-regulated Src/PI3K/Akt signaling pathway. SQLE facilitates cell proliferation, induces cell cycle progression, and inhibits apoptosis in vitro, while promoting tumor growth in vivo. SQLE inhibitors effectively suppress PC cell proliferation and xenograft tumor growth, highlighting the potential of SQLE as a therapeutic target for PC.
CELL DEATH & DISEASE
(2023)
Review
Virology
Tengyue Hu, Jiayi Wang, Weixiu Li, Miao Liu, Ning Han, Man Yuan, Lingyao Du, Hong Tang
Summary: This review article discusses the process initiated by hepatocytes through ER stress to combat HBV and HCV infection, and explains how this information can be beneficial in the treatment of HBV/HCV-related diseases.
Article
Multidisciplinary Sciences
Manoj Kumar, Harshit Garg, Nidhi Gupta, Alpana Sharma, Seema Kaushal, Rajeev Kumar, Amit Kumar Dinda
Summary: The study found that the mRNA and protein expression of GRP78 in tumor tissue samples of RCC patients were significantly higher compared to controls, as were the circulatory levels of GRP78 in serum samples. The expression of GRP78 correlated significantly with the pathological tumor stage and grade of RCC, suggesting its prognostic potential.
Article
Hematology
Malgorzata Furmanik, Rick van Gorp, Meredith Whitehead, Sadia Ahmad, Jayanta Bordoloi, Alexander Kapustin, Leon J. Schurgers, Catherine M. Shanahan
Summary: This study reveals that ER stress induces vascular calcification by increasing release of Grp78-loaded EVs, and for the first time links warfarin to promoting calcification by increasing EV release. The results of the study provide a novel mechanism of action for warfarin and the role of ER stress in cargo loading of EVs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Microbiology
Maria Guadalupe Martinez, Emmanuel Combe, Aurore Inchauspe, Philippe Emmanuel Mangeot, Elodie Delberghe, Fleur Chapus, Gregory Neveu, Antoine Alam, Kara Carter, Barbara Testoni, Fabien Zoulim
Summary: CRISPR-Cas9 targeting of HBV DNA leads to effective disruption of viral replication and the appearance of episomal HBV DNA variants. Sustainable effects induced by Cas9 post RNP degradation suggest permanent changes in the HBV genome. This study provides insights into the potential of CRISPR-Cas9 as a therapeutic approach for chronic hepatitis B by targeting the viral minichromosome.
Article
Cell Biology
Kalishwaralal Kalimuthu, Jin Hong Kim, Yong Seok Park, Xu Luo, Lin Zhang, Ja-Lok Ku, M. Haroon A. Choudry, Yong J. Lee
Summary: The combination treatment of glucose deprivation and TRAIL can induce apoptosis in tumor cells through the activation of various caspases, involving the ER stress response pathway and the mitochondria-dependent pathway.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pamela J. McFie, Paulos Chumala, George S. Katselis, Scot J. Stone
Summary: The study utilized CRISPR/Cas9 gene editing to add three consecutive FLAG epitopes to the C-terminus of endogenous DGAT2 in HepG2 cells, allowing successful detection of the protein at its endogenous locus within the cell.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Virology
Yueyuan Shi, Xin Jin, Shuang Wu, Junye Liu, Hongpeng Zhang, Xuefei Cai, Yuan Yang, Xiang Zhang, Jie Wei, Miao Luo, Hua Zhou, Huihao Zhou, Ailong Huang, Deqiang Wang
Summary: In this study, the mechanism of hepatitis B virus (HBV) enveloped particle release was investigated. Glucose-regulated protein (GRP)78 was identified as a specific binding target for preS1, and its interaction with preS1 was found to positively regulate the release of HBV-enveloped particles. Additionally, peptides inhibiting enveloped-particle release were identified as potential therapeutic agents for inhibiting HBV infection.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Cell Biology
Muntadher Al Zaidi, Vanessa Marggraf, Elena Repges, Georg Nickenig, Dirk Skowasch, Adem Aksoy, Carmen Pizarro
Summary: Cellular stress and inflammation play a role in pulmonary diseases, and GRP78 appears to be involved in the pathogenesis of these diseases. The study aimed to investigate the relevance of serum GRP78 in pulmonary disorders.
CELL STRESS & CHAPERONES
(2023)
Article
Oncology
Youngsic Jeon, Taejung Kim, Hyukjoon Kwon, Jeong-Kook Kim, Young-Tae Park, Jungyeob Ham, Young-Joo Kim
Summary: This study provides evidence that the combination treatment of cabozantinib and CBD leads to increased apoptosis in HCC cells. This enhanced apoptosis is mediated, at least in part, through the induction of ER stress and subsequent activation of the p53 pathway. However, there is no association found between the combination treatment and CNR.
Article
Agriculture, Dairy & Animal Science
Xueping Chen, Muhammad Ishfaq, Jian Wang
Summary: This study aimed to investigate the role of ceramide in the pathogenesis of Mycoplasma gallisepticum (MG). The results showed that MG infection induced ceramide accumulation in DF-1 cells, and inhibiting ceramide synthesis significantly inhibited MG proliferation and inflammatory injury. MG infection also caused endoplasmic reticulum stress, and inhibiting this stress prevented ceramide accumulation and MG proliferation, alleviating the inflammatory injury. The expression of STIM1 was elevated in response to MG infection, leading to calcium overload and oxidative stress. Treatment with baicalin downregulated STIM1 expression, restored calcium homeostasis, mitigated oxidative stress, and ameliorated the inflammatory injury caused by MG.
Article
Cell Biology
Philma Glora Muthuraj, Prakash K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan
Summary: ZIKV infection induces apoptosis in placental trophoblasts, possibly through triggering endoplasmic reticulum stress and activating the JNK pathway, while inhibition of JNK significantly prevents ZIKV-induced apoptosis.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Terunao Iwanaga, Tetsuhiro Chiba, Masato Nakamura, Tatsuya Kaneko, Junjie Ao, Na Qiang, Yaojia Ma, Jiaqi Zhang, Tadayoshi Kogure, Sae Yumita, Takamasa Ishino, Keita Ogawa, Motoyasu Kan, Miyuki Nakagawa, Kisako Fujiwara, Naoto Fujita, Takafumi Sakuma, Hiroaki Kanzaki, Keisuke Koroki, Yuko Kusakabe, Masanori Inoue, Kazufumi Kobayashi, Naoya Kanogawa, Soichiro Kiyono, Takayuki Kondo, Ryo Nakagawa, Sadahisa Ogasawara, Shingo Nakamoto, Ryosuke Muroyama, Jun Kato, Tatsuo Kanda, Hitoshi Maruyama, Naoya Mimura, Takuya Honda, Toshihiko Murayama, Hiroyuki Nakamura, Naoya Kato
Summary: This study demonstrates that miglustat can suppress liver fibrosis by inhibiting the TGF-5/Smad pathway, reducing the expression of extracellular matrix components such as collagen. The research also shows that miglustat can both prevent the occurrence of liver fibrosis and reverse established fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Keiichi Fujiwara, Shin Yasui, Takayuki Kondo, Masato Nakamura, Makoto Arai, Tatsuo Kanda, Osamu Yokosuka, Masayuki Ohtsuka, Ryuzo Abe, Naoya Kato
Summary: The prognosis of patients with fulminant hepatitis and late-onset hepatic failure in Japan has not improved over the past 32 years, despite advancements in clinical management and liver transplantation. The increasing proportion of patients with poor prognosis and difficult-to-treat etiologies, as well as the aging population, may contribute to this lack of improvement.
HEPATOLOGY RESEARCH
(2023)
Article
Virology
Reina Sasaki-Tanaka, Ryota Masuzaki, Hiroaki Okamoto, Toshikatsu Shibata, Mitsuhiko Moriyama, Hirofumi Kogure, Tatsuo Kanda
Summary: This study identified nicotinamide as a potential therapeutic drug for HAV infection by inhibiting HAV replication through suppressing AP-1 activity and HAV IRES-mediated translation.
JOURNAL OF VIROLOGY
(2023)
Article
Surgery
Hayato Abe, Kazu Shibutani, Shintaro Yamazaki, Tatsuo Kanda, Mitsuhiko Moriyama, Masahiro Okada, Masahiko Sugitani, Shingo Tsuji, Tadatoshi Takayama, Yukiyasu Okamura
Summary: This study aimed to investigate the predictive value of preoperative tumor stiffness measured by magnetic resonance elastography on overall survival and recurrence-free survival in patients with hepatocellular carcinoma. The results showed that higher tumor stiffness was associated with increased risk of recurrence and death.
Article
Gastroenterology & Hepatology
Satoshi Takakusagi, Hitoshi Takagi, Yuichi Yamazaki, Takashi Kosone, Shigeo Nagashima, Masaharu Takahashi, Kazumoto Murata, Hiroaki Okamoto
Summary: The article describes a case of autochthonous chronic hepatitis E in a woman in her late 70s who was initially misdiagnosed as having autoimmune hepatitis, but was later found to be caused by hepatitis E virus infection.
CLINICAL JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Oncology
Keita Ogawa, Tetsuhiro Chiba, Masato Nakamura, Jun Arai, Jiaqi Zhang, Yaojia Ma, Na Qiang, Junjie Ao, Sae Yumita, Takamasa Ishino, Motoyasu Kan, Terunao Iwanaga, Miyuki Nakagawa, Kisako Fujiwara, Takafumi Sakuma, Hiroaki Kanzaki, Keisuke Koroki, Yuko Kusakabe, Kazufumi Kobayashi, Naoya Kanogawa, Soichiro Kiyono, Takayuki Kondo, Ryo Nakagawa, Sadahisa Ogasawara, Ryosuke Muroyama, Shingo Nakamoto, Tatsuo Kanda, Hitoshi Maruyama, Jun Kato, Shoji Matsumoto, Takayoshi Arai, Shinichiro Motohashi, Naoya Kato
Summary: In this study, the inhibition of ADAM9 was found to prevent the release of MICA and activate the immune function of NK cells. Through the screening of a chemical library, a compound called CCL347 with five benzene rings was identified, which significantly reduced sMICA levels in HCC cell lines and enhanced NK cell cytotoxicity, indicating its potential as a novel therapeutic drug for HCC.
ANTICANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Reina Sasaki-Tanaka, Toshikatsu Shibata, Mitsuhiko Moriyama, Hirofumi Kogure, Asuka Hirai-Yuki, Hiroaki Okamoto, Tatsuo Kanda
Summary: In this study, HuhT7-HAV/Luc cells were established as a suitable model for anti-HAV drug screening. Masitinib, a tyrosine kinase inhibitor, was found to reduce HAV replication and internal ribosomal entry-site (IRES) activity. It may be a potential treatment for severe HAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shini Kanezawa, Mitsuhiko Moriyama, Tatsuo Kanda, Akiko Fukushima, Ryota Masuzaki, Reina Sasaki-Tanaka, Akiko Tsunemi, Takahiro Ueno, Noboru Fukuda, Hirofumi Kogure
Summary: Metabolic-dysfunction-associated fatty-liver disease (MAFLD) is the leading cause of liver disease worldwide. Nonalcoholic steatohepatitis (NASH) patients have a higher prevalence of small-intestinal bacterial overgrowth (SIBO). In this study, we investigated the differences in gut microbiota between stroke-prone spontaneously hypertensive-5 rats (SHRSP5) fed a normal diet (ND) or a high-fat- and high-cholesterol-containing diet (HFCD). We found an association between MAFLD and gut microbiota alteration, suggesting that targeting gut microbiota may be a potential therapeutic approach for MAFLD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Putu Prathiwi Primadharsini, Shigeo Nagashima, Toshinori Tanaka, Suljid Jirintai, Masaharu Takahashi, Kazumoto Murata, Hiroaki Okamoto
Summary: HCV-1, a major cause of acute viral hepatitis, has been difficult to study due to its poor replication in cultured cells. A cDNA clone was successfully constructed and an infectious virus was produced, providing a useful tool for studying HCV-1 replication and severe hepatitis in infected pregnant women, as well as for developing safer treatment options.
Article
Gastroenterology & Hepatology
Tatsuo Kanda, Reina Sasaki-Tanaka, Koji Ishii, Ryosuke Suzuki, Jun Inoue, Atsunori Tsuchiya, Shingo Nakamoto, Ryuzo Abe, Keiichi Fujiwara, Osamu Yokosuka, Tian-Cheng Li, Satoshi Kunita, Hiroshi Yotsuyanagi, Hiroaki Okamoto
Summary: The Japan Agency for Medical Research and Development (AMED) Hepatitis A and E viruses (HAV and HEV) Study Group has reviewed the recent advances in HAV research and clinical practice for HAV infection in Japan and authored comprehensive guidelines. The article covers virological findings, clinical presentation, pathogenicity, diagnostic approaches, complications, risk factors, preventive measures, and treatment modalities for hepatitis A.
HEPATOLOGY RESEARCH
(2023)
Article
Gastroenterology & Hepatology
Yuichi Yoshida, Asami Ito, Hisashi Eto, Akiko Suzuki, Tamami Abe, Kei Endo, Keisuke Kakisaka, Takayoshi Oikawa, Hidekatsu Kuroda, Akio Miyasaka, Takayuki Matsumoto, Masaharu Takahashi, Hiroaki Okamoto
Summary: This study surveyed the current seroprevalence and incidence of hepatitis E virus (HEV) infection among the general population in Iwate Prefecture, Japan. The findings suggest that the incidence of HEV infection is high and it is a leading cause of hepatitis virus infection in this region.
HEPATOLOGY RESEARCH
(2023)
Article
Virology
Shigeo Nagashima, Putu Prathiwi Primadharsini, Takashi Nishiyama, Masaharu Takahashi, Kazumoto Murata, Hiroaki Okamoto
Summary: This study developed a reporter hepatitis E virus (HEV) and demonstrated its efficient replication, genetic stability, and infectivity. The researchers also simplified the measurement of luciferase activity and used it to evaluate the effects of introducing small interfering RNAs and inhibitors on HEV release. These findings contribute to the understanding of the HEV life cycle and provide a more convenient screening platform for candidate drugs targeting the late stage of HEV infection.
JOURNAL OF VIROLOGY
(2023)
Review
Virology
Putu Prathiwi Primadharsini, Shigeo Nagashima, Takashi Nishiyama, Hiroaki Okamoto
Summary: Hepatitis E virus (HEV) is recognized as the main cause of acute hepatitis, and the lack of specific antiviral drugs for HEV is a challenge. Researchers have established reporter systems to target different steps of the HEV life cycle and identified potential drug candidates. In vitro tests using a robust cell culture system confirmed the efficacy of these drugs alone or in combination with existing drugs.
Article
Virology
Masaharu Takahashi, Satoshi Kunita, Tsutomu Nishizawa, Hiroshi Ohnishi, Putu Prathiwi Primadharsini, Shigeo Nagashima, Kazumoto Murata, Hiroaki Okamoto
Summary: This study investigated the infection dynamics and genomic mutations of HEV in domestic pigs on a farrow-to-finish pig farm in Japan between 2012 and 2021. The results showed active viral replication of HEV in pigs and the presence of HEV RNA in the farm environment. All HEV strains belonged to subgenotype 3b, with a gradual decrease in nucleotide identities over time. Importantly, one HEV strain demonstrated infectivity in human hepatoma cells. These findings contribute to our understanding of HEV prevalence, transmission dynamics, and genetic characteristics.
