Article
Cell Biology
Jin Hong Kim, Abdo J. Najy, Jian Li, Xu Luo, Hyeong-Reh C. Kim, Mohammad Haroon Asif Choudry, Yong J. Lee
Summary: This study investigated the crosstalk between the ferroptotic agent-induced endoplasmic reticulum (ER) stress response and TRAIL-induced apoptosis, and found that Bid plays a critical role in this process.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Bo Pan, Jing Sun, Ziyu Liu, Lingxiao Wang, Huixia Huo, Yunfang Zhao, Pengfei Tu, Wei Xiao, Jiao Zheng, Jun Li
Summary: In this study, LTC was found to protect against ischemia/reperfusion injury by inhibiting ER stress and MAPK-mediated mechanisms in PC12 cells and animal models. The anti-apoptotic effect of LTC was demonstrated by suppressing the cleavage of PARP, caspase-3, and caspase-9, as well as inhibiting the activation of PERK/eIF2α and IRE1/TRAF2 pathways.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Medicine, Research & Experimental
Federica Laudisi, Teresa Pacifico, Claudia Maresca, Anderson Luiz-Ferreira, Sara Antonelli, Angela Ortenzi, Alfredo Colantoni, Antonio Di Grazia, Eleonora Franze, Marco Colella, Davide Di Fusco, Giuseppe S. Sica, Ivan Monteleone, Giovanni Monteleone, Carmine Stolfi
Summary: Colorectal cancer is a major cause of cancer-related death, and current treatments are not effective against advanced disease. Researchers have found that the anthelmintic drug rafoxanide can enhance the sensitivity of colorectal cancer cells to TRAIL, a targeted therapy. This discovery suggests that rafoxanide could be a potential treatment option for overcoming TRAIL resistance in colorectal cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Sumit Sen Santara, Dian-Jang Lee, Angela Crespo, Jun Jacob Hu, Caitlin Walker, Xiyu Ma, Ying Zhang, Sourav Chowdhury, Karla F. Meza-Sosa, Mercedes Lewandrowski, Haiwei Zhang, Marjorie Rowe, Arthur McClelland, Hao Wu, Caroline Junqueira, Judy Lieberman
Summary: Natural killer (NK) cells can recognize externalized calreticulin (ecto-CRT) as an endogenous ligand for the activating receptor NKp46. Recognition of ecto-CRT by NKp46 triggers NK cell signaling and leads to the elimination of ER-stressed cells. This recognition mechanism plays a crucial role in immune surveillance and cancer prevention.
Article
Chemistry, Multidisciplinary
Shoaib Manzoor, Daniyah A. Almarghalani, Antonisamy William James, Md Kausar Raza, Tasneem Kausar, Shahid M. Nayeem, Nasimul Hoda, Zahoor A. Shah
Summary: This study found that triazole-pyrimidine hybrid compounds exhibit neuroprotective and anti-inflammatory properties. Several compounds showed significant anti-neuroinflammatory activity by inhibiting the production of nitric oxide and tumor necrosis factor-alpha in human microglia cells. Additionally, several compounds demonstrated promising neuroprotective activity in human neuronal cells by reducing the expression of endoplasmic reticulum chaperone BIP and apoptosis marker cleaved caspase-3.
PHARMACEUTICAL RESEARCH
(2023)
Article
Environmental Sciences
Yuanyuan Zhou, Hanyu Liao, Shanshan Yin, Pengqiao Wang, Xiaoqing Ye, Jianyun Zhang
Summary: Organophosphate esters (OPEs) are widely used man-made chemicals detected in various human-related samples. This study evaluated the toxicity and mechanisms of nine commonly detected OPEs on human hepatocellular carcinomas. The OPEs induced apoptosis, oxidative stress, endoplasmic reticulum stress, and NLRP3 inflammasome activation. Different types of OPEs showed varying effects on ROS overproduction, DNA damage, and cytotoxicity.
ENVIRONMENTAL POLLUTION
(2023)
Article
Endocrinology & Metabolism
Min-min Guo, Sheng-biao Qu, Hui-ling Lu, Wen-bo Wang, Mu-Liang He, Jian-Lin Su, Jian Chen, Yong Wang
Summary: Our study demonstrates that biochanin A can alleviate cerebral I/R-induced damage by suppressing apoptosis, ER stress, and p38 MAPK signaling.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biology
Ying Jia, Lian Yi, Qianqian Li, Tingjiao Liu, Shanshan Yang
Summary: This study found that MALAT1 expression was significantly increased in cerebral ischemia/reperfusion injury, and it promoted neuronal injury through the miR-195a-5p/HMGA1 pathway. This discovery reveals the mechanism of MALAT1 involvement in cerebral ischemia/reperfusion injury.
BIOLOGICAL RESEARCH
(2021)
Article
Neurosciences
Jiang-Wen Yin, Jia Li, Yi-Min Ren, Yan Li, Rui-Xue Wang, Sheng Wang, Yun-Xia Zuo
Summary: The study evaluated the effect of combination therapy with dexmedetomidine and Netrin-1 against ischemic brain injury, finding that the combination therapy can reduce infarct volume and neural damage, decrease apoptosis, and improve OPS recovery rate. The combination therapy also lowers the expression of GRP78 and CHOP, increases MEF2A expression, and promotes ERK5 phosphorylation.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jinghuan Wang, Jie Xu, Yejun Dong, Zhenghua Su, Haibi Su, Qianwen Cheng, Xinhua Liu
Summary: Ischemic stroke leads to protein misfolding, calcium imbalance, impaired ER function, and activation of the UPR. This study demonstrates that PARP16 plays a crucial role in the post-ischemic UPR and knocking down PARP16 alleviates brain injury after ischemic stroke. PARP16 functions as an ADP-ribosyltransferase to modulate the UPR through PERK and IRE1α, and its overexpression promotes ER stress-mediated cell damage.
Article
Integrative & Complementary Medicine
Wen Jiang, Wei Zhang, Yuxiang Zhang, Hao Yang, Xiaomei Pan, Qiang Chen, Junhui Chen
Summary: The study investigated the effects of tilianin extracted from Xiangqinglan on the apoptosis of H9c2 cells after oxygen glucose deprivation/reoxygenation (OGD/R), and found that it could inhibit OGD/R-induced cell apoptosis through the mitochondrial pathway and endoplasmic reticulum stress.
JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2023)
Article
Ophthalmology
Xiaorui Wang, Xinxing Zhu, Guangqian Huang, Lili Wu, Zhiyong Meng, Yuyu Wu
Summary: This study investigated the relationship between endoplasmic reticulum stress and Muller glial cells during retinal ischemia and hypoxia. The downregulation of PERK expression had an anti-apoptotic effect on OGD-conditioned rMC-1 cells, suggesting a possible mechanism of MG cell apoptosis during retinal ischemic injury.
Article
Biochemistry & Molecular Biology
Teresa Montiel, Juan Carlos Gomora-Garcia, Cristian Geronimo-Olvera, Yessica Heras-Romero, Berenice N. Bernal-Vicente, Xochitl Perez-Martinez, Luis B. Tovar-y-Romo, Lourdes Massieu
Summary: Ischemic stroke is a main cause of disability worldwide, and there is no simple treatment for ischemic brain injury. However, the D enantiomer of beta-hydroxybutyrate (BHB) has a protective effect by stimulating autophagic flux and inhibiting the activation of the unfolded protein response (UPR), while the L enantiomer has no significant effect.
JOURNAL OF NEUROCHEMISTRY
(2023)
Review
Immunology
Jana Messias Sandes, Regina Celia Bressan Queiroz de Figueiredo
Summary: The endoplasmic reticulum (ER) is an important organelle in higher eukaryotic cells responsible for protein folding and assembly. It undergoes strict quality control to ensure proper processing of proteins, with unfolded proteins being targeted for degradation through the ER-associated degradation (ERAD) complex. Additionally, the ER responds to stress through the unfolded protein response (UPR) to restore homeostasis or induce cell death. Protozoan parasites such as Trypanosoma cruzi, Trypanosoma brucei, and Leishmania spp. also have similar ER structures and stress response mechanisms, making them attractive targets for chemotherapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Xiaoping Song, Xiangting Xu, Jiali Lu, Xiaoyuan Chi, Yue Pang, Qingwei Li
Summary: The novel Lamprey immune protein (LIP) derived from Lampetra japonica efficiently kills lung cancer cells by inducing cell membrane decomposition and activating the ER stress pathway, leading to apoptosis. Moreover, injection of LIP significantly inhibits tumor growth in nude mice, suggesting its potential application in lung cancer treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medical Laboratory Technology
Ashok K. Dilly, Brendon D. Honick, Robin Frederick, Anulekha Elapavaluru, Sachin Velankar, Hima Makala, T. Kevin Hitchens, Lesley M. Foley, Jianxia Guo, Jan H. Beumer, Lora Heather Rigatti, Yong J. Lee, David L. Bartlett, Haroon A. Choudry
Summary: In patients with appendiceal mucinous carcinoma peritonei, abundant intraperitoneal accumulation of extracellular mucus causes organ dysfunction and hampers chemotherapeutic drug delivery. Combination of bromelain and N-acetylcysteine efficiently lyses extracellular mucus, reduces tumor-related symptoms, and enhances the efficacy of chemotherapy.
TRANSLATIONAL RESEARCH
(2021)
Article
Engineering, Biomedical
Haozhe Huang, Yixian Huang, Yuang Chen, Zhangyi Luo, Ziqian Zhang, Runzi Sun, Zhuoya Wan, Jingjing Sun, Binfeng Lu, Lin Zhang, Jing Hu, Song Li
Summary: COX inhibition plays a crucial role in cancer therapy, and repurposing 5-ASA as a COX inhibitor in combination with DOX can improve antitumor activity. The development of FASA/DOX may represent a new and effective immunochemotherapy for various types of cancers.
Article
Biochemistry & Molecular Biology
Rochelle Fletcher, Jingshan Tong, Denise Risnik, Brian J. Leibowitz, Yi-Jun Wang, Fernando Concha-Benavente, Jonathan M. DeLiberty, Donna B. Stolz, Reet K. Pai, Robert L. Ferris, Robert E. Schoen, Jian Yu, Lin Zhang
Summary: The study reveals that NSAIDs induce endoplasmic reticulum stress and activate BID protein to suppress tumor formation, and the immunogenic cell death effect induced by NSAIDs plays a crucial role in tumor suppression.
Article
Oncology
Jingshan Tong, Xiao Tan, Xiangping Song, Man Gao, Denise Risnik, Suisui Hao, Kaylee Ermine, Peng Wang, Hua Li, Yi Huang, Jian Yu, Lin Zhang
Summary: This study found that CDK4/6 inhibitors promote immunogenic cell death of cancer cells by regulating the expression of p73 and DR5, enhancing the effects of chemotherapy and immune therapy.
Review
Biochemistry & Molecular Biology
Kalishwaralal Kalimuthu, Chenicheri K. Keerthana, Manikandan Mohan, Jaison Arivalagan, Johnson Retnaraj Samuel Selvan Christyraj, Michael A. Firer, Mohammad Haroon Asif Choudry, Ruby John Anto, Yong J. Lee
Summary: This review critically analyzes the molecular mechanisms of selenium metabolism and its potential as a therapeutic target for cancer, highlighting SECISBP2 as a novel prognostic predictor and an alternative target for cancer. Strategies to develop a novel selenium metabolism-based approach for cancer therapy are emphasized, with expectations for extensive clinical use of SECISBP2 as a specific biomarker in cancer therapy. Scientists face additional challenges, including research on anticancer peptides targeting the intracellular protein SECISBP2.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jingshan Tong, Xiao Tan, Denise Risnik, Man Gao, Xiangping Song, Kaylee Ermine, Liangfang Shen, Shaomeng Wang, Jian Yu, Lin Zhang
Summary: The study shows that inducing BET protein degradation through PROTAC approach can effectively suppress the growth of colorectal cancer by activating DR5-mediated immunogenic cell death. Additionally, DR5 is crucial for the potent antitumor effect of combining BET degradation and anti-PD-1 antibody.
Article
Multidisciplinary Sciences
Brian J. Leibowitz, Guangyi Zhao, Liang Wei, Hang Ruan, Michael Epperly, Lujia Chen, Xinghua Lu, Joel S. Greenberger, Lin Zhang, Jian Yu
Summary: The cGAS-STING-dependent type I interferon response plays a critical role in driving intestinal regeneration and aiding in recovery from radiation injury. Deficiency in the STING pathway impairs epithelial IFN-beta production, inflammation, transcriptional response, and subsequent crypt regeneration, leading to impaired intestinal regeneration and survival.
Article
Biochemistry & Molecular Biology
Weilin Liu, Hongqi Chen, Zhi Zhu, Zuqiang Liu, Congrong Ma, Yong J. Lee, David L. Bartlett, Zong-Sheng Guo
Summary: The study found that the combination of the ferroptosis activator Erastin with the oncolytic vaccinia virus could effectively inhibit tumor growth, prolong host survival, and enhance antitumoral immunity in hepatocellular carcinoma and colon cancer models.
Article
Cell Biology
Jin Hong Kim, Abdo J. Najy, Jian Li, Xu Luo, Hyeong-Reh C. Kim, Mohammad Haroon Asif Choudry, Yong J. Lee
Summary: This study investigated the crosstalk between the ferroptotic agent-induced endoplasmic reticulum (ER) stress response and TRAIL-induced apoptosis, and found that Bid plays a critical role in this process.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Oncology
Chaoyuan Kuang, Jingshan Tong, Kaylee Ermine, Manbo Cai, Fujun Dai, Suisui Hao, Francis Giles, Yi Huang, Jian Yu, Lin Zhang
Summary: The study demonstrates that NEO2734 more potently suppresses CRC cell growth than first generation BET inhibitors, inducing CRC cell apoptosis via both the intrinsic and extrinsic pathways. NEO2734 treatment simultaneously upregulates PUMA and DR5 to induce cell death, effectively suppressing CRC growth.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jingshan Tong, Xiao Tan, Suisui Hao, Kaylee Ermine, Xinyan Lu, Zhaojin Liu, Anupma Jha, Jian Yu, Lin Zhang
Summary: Targeting cyclin-dependent kinases (CDKs) has shown promising therapeutic potential against cancer. A recent study reveals that selective CDK4/6 inhibitors induce Death Receptor 5 (DR5) via p73, leading to increased sensitivity of colorectal cancer cells to therapy-induced apoptosis. This finding provides valuable insights into the anticancer mechanisms of CDKIs and their potential clinical applications in colorectal cancer.
Article
Multidisciplinary Sciences
Suisui Hao, Jingshan Tong, Anupma Jha, Denise Risnik, Darleny Lizardo, Xinyan Lu, Ajay Goel, Patricia L. Opresko, Jian Yu, Lin Zhang
Summary: Synthetic lethality can be used to target oncogenic drivers in cancer, and in this study, it was found that WRN helicase is required for the survival of MSI CRC cells. Depletion of WRN induces p53 and PUMA, leading to apoptosis in MSI CRC cells. The vulnerability of MSI CRCs to WRN loss is mediated by p53/PUMA-dependent apoptosis. WRN depletion or treatment with ML216 suppresses the growth of MSI CRCs in vitro and in vivo in a p53/PUMA-dependent manner.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Kaylee Ermine, Jian Yu, Lin Zhang
Summary: The RIP kinase family plays a crucial role in cell survival and cell death signaling. Dysregulation of RIP kinases is associated with various pathological conditions including inflammatory diseases, neurological diseases, and cancer. Alterations of RIP kinases in cancer cells contribute to tumor progression, therapeutic resistance, and escape from immune response. Targeting RIP kinases has potential implications for cancer treatment.
Article
Biochemistry & Molecular Biology
Hang Ruan, Brian J. Leibowitz, Yingpeng Peng, Lin Shen, Lujia Chen, Charlie Kuang, Robert E. Schoen, Xinghua Lu, Lin Zhang, Jian Yu
Summary: Mutant KRAS plays a crucial role in colorectal cancer by promoting Myc translation and Myc-dependent stress adaptation and proliferation. The combination of two FDA-approved drugs, Bortezomib and Everolimus, has been found to be highly effective against mutant KRAS colorectal cancer cells. This combination rapidly depletes Myc protein and induces cell death through various pathways. The study also shows that mutant KRAS colorectal cancer cells with elevated basal Myc and p-eIF2 alpha are more sensitive to the BR combination and exhibit characteristics of stress adaptation and cell death.
MOLECULAR BIOMEDICINE
(2022)