Review
Biochemistry & Molecular Biology
Ruipeng Wang, Tiankang Guo, Junliang Li
Summary: Peritoneal adhesion (PA) is a fibrotic tissue that connects abdominal or visceral organs to the peritoneum, and its formation can lead to various clinical diseases. Damage to peritoneal mesothelial cells (PMCs) is believed to be one of the causes of PA. This review aims to understand the mechanism of action of PMCs in PA and provide a theoretical foundation for the treatment and prevention of PA.
Article
Biochemistry & Molecular Biology
Guoqing Qian, Oluwaseun Adeyanju, Saptarshi Roy, Christudas Sunil, Ann Jeffers, Xia Guo, Mitsuo Ikebe, Steven Idell, Torry A. Tucker
Summary: DOCK2 plays an important role in pleural fibrosis by mediating mesothelial to mesenchymal transition (MesoMT) and deposition of neomatrix. DOCK2 may represent a novel target for the prevention or treatment of pleural fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yue Pu, Yao Wu, Yan Zhou, Li-hong Wan
Summary: Azithromycin (AZM) targets RACK1 to inhibit TGF-beta 1 mediated EMT in bronchial epithelial cells, leading to the suppression of cell migration and reversal of EMT.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Chemistry, Medicinal
Yifan Wang, Yixiang Wang, Yang Wu, Yiqing Wang
Summary: The study aimed to investigate the effect of dulaglutide on intrauterine adhesion (IUA) and elucidate its mechanism for potential clinical treatment of IUA. The findings showed that dulaglutide improved uterine cavity shape, increased endometrial thickness and gland number, and significantly reduced collagen fiber deposition in the endometrium. It also reduced inflammatory responses, inhibited M1 macrophage polarization and inflammatory factor release, and ameliorated fibrosis via inhibiting epithelial-mesenchymal transition (EMT) through the TGF-beta/Smad2 signaling pathway.
Article
Oncology
Hyun-Wook Lee, Cynthia C. Jose, Suresh Cuddapah
Summary: Nickel compounds, prevalent in the atmosphere due to industrial processes and fossil fuel burning, are associated with chronic inflammatory lung diseases, including asthma and lung cancer, primarily through the mechanism of epithelial-mesenchymal transition (EMT).
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Article
Biochemistry & Molecular Biology
Lauren A. Griggs, Christopher A. Lemmon
Summary: Epithelial to Mesenchymal Transition (EMT) is a process in which epithelial cells transition to a motile and invasive mesenchymal phenotype. The EMT process is associated with an increase in fibronectin (FN) assembly and the localization of Transforming Growth Factor-beta 1 (TGF-beta 1) to FN fibrils. This study investigated the spatial gradients of EMT and FN fibrillogenesis in mammary epithelial cells cultured on square micropatterns in response to TGF-beta 1. The results showed that increasing TGF-beta 1 concentration induced a spatial gradient of both E-Cadherin and FN fibrils, and inhibition of FN fibril assembly globally reduced both E-Cadherin-containing adherens junctions and FN fibrillogenesis, but did not eliminate the spatial gradients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Le Phuong Hoang Anh, Ken Nishimura, Akihiro Kuno, Nguyen Thuy Linh, Tetsuo Kato, Manami Ohtaka, Mahito Nakanishi, Eiji Sugihara, Taka-Aki Sato, Yohei Hayashi, Aya Fukuda, Koji Hisatake
Summary: Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). In this study, the researchers found that the downregulation of Osr2 is crucial for efficient reprogramming by using a cell-based model. Osr2 acts as a novel EMT regulator and its downregulation diminishes TGF-beta signaling and activates Wnt signaling, promoting MET and acquiring pluripotency.
Review
Biochemistry & Molecular Biology
Margherita Sisto, Domenico Ribatti, Sabrina Lisi
Summary: Recent advances in understanding the molecular pathways linking inflammation with organ fibrosis and autoimmune diseases have identified EMT as a common association. The chronic inflammatory microenvironment plays a crucial role in inducing pathological EMT, with TGF-beta being a key player in fibrosis induction. Further research aims to target EMT-dependent fibrosis in autoimmune diseases through a better understanding of molecular responses to inflammatory signals.
Article
Medicine, Research & Experimental
Ning Zhao, Minwei He, Wei Chen, Peng Jin, Lulu Cao, Jinhai Deng, Xu Cheng, Lu Wang
Summary: Our study identified FAM96A as a crucial regulator of tumor metastasis and epithelial-mesenchymal transition (EMT) by inhibiting the TGF-beta signaling pathway.
Article
Multidisciplinary Sciences
Shunhao Wang, Jingchao Li, Mei Chen, Liting Ren, Wenya Feng, Lining Xu, Xiaolan Chen, Tian Xia, Nanfeng Zheng, Sijin Liu
Summary: Palladium nanoplates inhibit lung metastasis by suppressing EMT signaling in breast cancer cells and interacting with TGF-beta proteins, leading to reduced downstream signaling activation. NIR irradiation enhances the inhibitory effects of PdPL on both primary tumors and metastasis.
NATIONAL SCIENCE REVIEW
(2021)
Article
Biophysics
Naba Mukhtar, Eric N. Cytrynbaum, Leah Edelstein-Keshet
Summary: Epithelial-mesenchymal transition (EMT) plays a crucial role in wound healing, embryonic development, and cancer metastasis. This study presents a multiscale model that investigates the role of the protein YAP in promoting EMT and validates the simulation results with experimental observations.
BIOPHYSICAL JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Yingfeng Shi, Min Tao, Jun Ni, Lunxian Tang, Feng Liu, Hui Chen, Xiaoyan Ma, Yan Hu, Xun Zhou, Andong Qiu, Shougang Zhuang, Na Liu
Summary: IL-6-induced HDAC6 not only regulated IL-6 downstream JAK2/STAT3 signaling, but also co-activated TGF-beta/Smad3 signaling, leading to changes in the phenotype and mobility of HPMCs. HDAC6 could be a potential therapeutic target for preventing and treating peritoneal fibrosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yoshiko Hashimoto, Tomoko Tsuzuki-Nakao, Naoko Kida, Yoshiyuki Matsuo, Tetsuo Maruyama, Hidetaka Okada, Kiichi Hirota
Summary: The endometrium undergoes repeated changes during the menstrual cycle, and chronic endometritis can lead to implantation failure and miscarriage. In this study, the effects of inflammatory cytokines, hypoxia-inducible factor (HIF), and inflammation on endometrial epithelial cells were investigated. The results showed that pro-inflammatory factors and hypoxia activated HIF-1 and promoted epithelial-mesenchymal transition (EMT) in endometrial epithelial cells.
Review
Genetics & Heredity
Henriett Butz, Gabor Nyiro, Petra Anna Kurucz, Istvan Liko, Attila Patocs
Summary: Congenital hypogonadotropic hypogonadism (CHH) is a genetically heterogeneous congenital disease with over 40 identified pathogenic genes. High-throughput next-generation sequencing (NGS) allows for efficient analysis, but interpretation of genetic data remains challenging due to the complex genetics of CHH.
Review
Immunology
Vince Kornel Grolmusz, Aniko Bozsik, Janos Papp, Attila Patocs
Summary: The ongoing COVID-19 pandemic, caused by the novel coronavirus, has impacted all aspects of human society, with a special focus on healthcare. SNPs play a crucial role in influencing the viral entry of SARS-CoV-2 and innate immunity, potentially altering susceptibility to infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Henriett Butz, Janos Papp, Aniko Bozsik, Lilla Krokker, Timea Pocza, Edit Olah, Attila Patocs
Summary: The potential pathogenic role of germline BRCA2 c.9976A>T and c.10095delinsGAATTATATCT variants in hereditary breast and ovarian cancer (HBOC) patients was evaluated. Both variants showed no significant association with clinicopathological parameters or elevated risk for HBOC tumors. Families carrying c.9976A>T were more prone to lung cancer, while an increased prevalence of pancreatic cancer was found in families with c.9976A>T and other pathogenic BRCA1 variants. Further research in larger sample cohorts is needed for validation.
Review
Genetics & Heredity
Lilla Krokker, Attila Patocs, Henriett Butz
Summary: The 14q32 miRNAs play a significant role in the tumorigenesis of endocrine organs and have potential as biomarkers in endocrine neoplasms. Different endocrine tumors exhibit distinct characteristics in the expression of 14q32 miRNAs, suggesting further research may aid in diagnosis and prognosis.
Article
Oncology
Balazs Sarkadi, Istvan Liko, Gabor Nyiro, Peter Igaz, Henriett Butz, Attila Patocs
Summary: The study demonstrates the evolution and effectiveness of Next Generation Sequencing (NGS) techniques, particularly whole exome sequencing and targeted panel sequencing, in diagnosing hereditary cancer syndromes. NGS-based methods are efficient and cost-effective tools for molecular genetic diagnosis, especially for assessing mutations in pheochromocytoma/paraganglioma (PPGL) associated genes. Different library preparations, sequencing platforms, and bioinformatical settings significantly impact the diagnostic accuracy, with an in-house developed targeted panel sequencing showing potential for identifying genetic susceptibility of PPGL.
Article
Cell Biology
Luca Pagliaroli, Abel Fothi, Ester Nespoli, Istvan Liko, Borbala Veto, Piroska Devay, Flora Szeri, Bastian Hengerer, Csaba Barta, Tamas Aranyi
Summary: Dyskinesias are abnormal repetitive involuntary movements caused by dysfunctional neuronal activity. While levodopa-induced dyskinesia has no clinical treatment for Parkinson's disease patients, Riluzole shows potential to improve the phenotype by affecting DNA methylation. The study suggests that Riluzole co-treatment may pinpoint specific methylation targets for potential new druggable targets in Levodopa-Induced Dyskinesia.
Article
Biochemistry & Molecular Biology
Henriett Butz, Katalin Meszaros, Istvan Liko, Attila Patocs
Summary: The study found that glucocorticoid treatment led to miRNA expression changes in different cell types. 19.5% of the influenced signaling pathways were common in all experiments, with the Wnt signaling pathway being the most affected. Transcriptome data and tissue-specific target prediction showed similar results, indicating significant alterations in the Wnt pathway in both the GC-producing adrenal gland and all investigated GC target tissue types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Balazs Sarkadi, Eva Saskoi, Henriett Butz, Attila Patocs
Summary: Pheochromocytomas and paragangliomas are the most heritable endocrine tumors, most of which are benign and can be resolved through surgical removal. However, a small percentage are malignant with limited therapeutic options available.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Peter Istvan Turai, Zoltan Herold, Gabor Nyiro, Katalin Borka, Tamas Micsik, Judit Toke, Nikolette Szuecs, Miklos Toth, Attila Patocs, Peter Igaz
Summary: Histological differential diagnosis of adrenocortical adenoma and carcinoma is challenging, but miRNA combinations have been identified as highly sensitive and specific predictors for malignancy, which could supplement histological examination and enable preoperative adrenal biopsy in patients with suspicious tumors.
Article
Oncology
Barbara Matyasi, Gabor Petovari, Titanilla Danko, Henriett Butz, Istvan Liko, Peter Low, Isabelle Petit, Randa Bittar, Dominique Bonnefont-Rousselot, Zsolt Farkas, Tamas Szeniczey, Kinga Molnar, Krisztina Paloczi, Edit Buzas, Mathieu Boissan, Anna Sebestyen, Krisztina Takacs-Vellai
Summary: Communication between cancer and stromal cells through extracellular vesicles (EVs) is important for tumour progression. This study investigates the role of NME1 and NME2, proteins found in EVs, in the interplay between cancer and stromal cells. The findings suggest a link between NME-containing EVs and the regulation of tumour metabolism.
Article
Oncology
Peter Istvan Turai, Gabor Nyiro, Katalin Borka, Tamas Micsik, Istvan Liko, Attila Patocs, Peter Igaz
Summary: Distinguishing between adrenocortical adenoma and carcinoma is challenging, and additional diagnostic methods are needed. This study investigated the expression profiles of circRNAs in adrenocortical tumors using next-generation sequencing and RT-qPCR validation, and found that circPHC3 is overexpressed in carcinoma and adenoma. This is the first study to investigate circRNAs in adrenocortical tumors, and further research is needed to understand their biological and diagnostic significance.
Article
Biochemistry & Molecular Biology
Aniko Bozsik, Henriett Butz, Vince Kornel Grolmusz, Csaba Polgar, Attila Patocs, Janos Papp
Summary: This article investigates a difficult-to-detect APC gene mutation in familial adenomatous polyposis (FAP) and confirms its association with the disease through genome sequencing and RNA-based assays.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Cell Biology
Henriett Butz, Eva Saskoi, Lilla Krokker, Viktoria Vereczki, Alan Alpar, Istvan Liko, Erika Toth, Erika Szocs, Mihaly Cserepes, Katalin Nagy, Imre Kacskovics, Attila Patocs
Summary: This study aimed to uncover the context-dependent action of glucocorticoid receptors (GR) in breast cancer. Through multiple cohorts, it was found that GR expression is higher in ER- breast cancer cells and GR-regulated genes are mainly involved in cell migration. The study also revealed that GR action is independent of ligand availability, highlighting its ligand-independent role in breast cancer. Therefore, caution should be applied in the interpretation of immunohistochemistry results, and the importance of GR in disease progression, particularly in cell migration, is emphasized.
Article
Oncology
Henriett Butz, Petra Nagy, Janos Papp, Aniko Bozsik, Vince Kornel Grolmusz, Timea Pocza, Edit Olah, Attila Patocs
Summary: The detection rate and characteristics of pathogenic/likely pathogenic (P/LP) variants in PALB2 gene may vary between different populations. In this study, PALB2 was analyzed in three independent cohorts and P/LP PALB2 variants were found in 1.4% of breast and/or ovarian cancer patients and in only 0.17% of patients with other cancers. The most common variant in our patients was NM_024675.4:c.509_510delGA.
Article
Cell Biology
Mohamed Kodiha, Nabila Azad, Siwei Chu, Noah Crampton, Ursula Stochaj
Summary: This study reveals that oxidative stress induces the nuclear accumulation of RanBP1 and the signaling pathways mediated by EGFR and PKA play a role in regulating RanBP1 localization during stress. RanBP1 acts as a downstream effector of these signaling routes, positioning it at the intersection of important cellular signaling circuits.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2024)