Review
Biotechnology & Applied Microbiology
Zhiyao Yan, Li-Guo Zhu, Kun Meng, Wanxia Huang, Qiwu Shi
Summary: In this review, the authors discuss the research progress of Terahertz (THz) medical imaging for in vivo applications. They focus on the development of imaging contrast agents and novel imaging methods suitable for living organisms, and propose a roadmap for taking THz medical imaging from in vitro to in vivo.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Kai Wang, Wei Wang, Shi-Yu Chen, Jia-Cheng Guo, Jia-Heng Li, Yu-Shun Yang, Xiao-Ming Wang, Chen Xu, Hai-Liang Zhu
Summary: A Near-Infrared (NIR) fluorescent probe L was designed for real-time monitoring of sulfite in living cells and zebrafish, demonstrating high selectivity, a short response time, low cytotoxicity, and potential clinical diagnostic applications.
Article
Biophysics
Qian Zhang, Yanxia Wu, Qinfeng Xu, Fei Ma, Chun-yang Zhang
Summary: DNA methylation is a vital epigenetic modification associated with various human diseases. Novel biosensors have been developed for sensitive detection of DNA methylation in recent years.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Spectroscopy
Dan Chang, Zhonghua Zhao, Wenjing Niu, Lihong Shi, Yongxing Yang
Summary: This study introduces an eco-friendly synthesis method for carbon dots and a label-free sensing platform for ultrasensitive Fe3+ recognition. The CDs prepared through hydrothermal strategy exhibit exceptional stability and biocompatibility, making them suitable for multicolour imaging applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Analytical
Danni Wang, Wanyi Xue, Xiuyan Ren, Zhangrun Xu
Summary: This review focuses on the general sensing mechanisms behind advanced methods for detecting telomerase activity, which are predominantly classified into three categories: hybridization reaction between DNA probes and extended primers, guanine-rich tail of extended primers, and electronegativity of extended primers. Critically reviewed are advanced telomerase assays corresponding to each sensing mechanism, along with discussions on current limitations and future research directions in the field of telomerase activity analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tonghui Huang, Shirong Yan, Yongbo Yu, Yunsheng Xue, Yanyan Yu, Cuiping Han
Summary: In this study, a dual-responsive ratiometric fluorescent probe PTZ-H was designed and synthesized for the separate and simultaneous detection of hypochlorite and peroxynitrite. The probe exhibited high sensitivity, specificity, and biocompatibility, allowing for targeted imaging of the two species in living cells, zebrafish, and tissues.
ANALYTICAL CHEMISTRY
(2022)
Review
Cell Biology
Adam Shellard, Roberto Mayor
Summary: Researchers have proposed durotaxis as a mechanism driving directed migration, where cells follow gradients of extracellular mechanical stiffness. Despite the lack of evidence for its existence in vivo, durotaxis has become a active field of research. Further studies are needed to determine the extent to which durotaxis may occur in vivo.
DEVELOPMENTAL CELL
(2021)
Article
Chemistry, Multidisciplinary
Amanda K. East, Melissa Y. Lucero, Jefferson Chan
Summary: In vivo imaging is a powerful tool for studying biological processes and guiding the development of diagnostics and therapies for diseases. Recent advancements in in vivo ABS applications are focusing on activity-based sensing and near-infrared imaging modalities.
Review
Immunology
Dennis R. Burton
Summary: Neutralizing antibodies (nAbs) have become increasingly important in preventing and treating viral infections and guiding vaccine development. In vivo, nAbs can block viral entry to target cells and exert antiviral effects against viruses and infected cells. Interpreting in vivo results based on in vitro assays is challenging but crucial in choosing the most effective antibody and vaccine strategies. This review discusses the assays for and mechanisms of neutralizing antibodies against viruses as tools to understand and predict their activity in vivo when administered passively or induced by vaccination or infection.
NATURE REVIEWS IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Xiaoting Liu, Liyan Zhang, Ling Lu, Wei Jiang, Nan Zhang
Summary: A 3D DNAzyme walker was developed for in situ imaging and sensitive detection of telomerase activity, demonstrating successful application in different cell lines and discrimination between cancer cells and normal cells, as well as screening of telomerase inhibitors.
Article
Chemistry, Analytical
Alexander N. Vaneev, Petr Gorelkin, Olga O. Krasnovskaya, Roman A. Akasov, Daniil Spector, Elena Lopatukhina, Roman Timoshenko, Anastasiia S. Garanina, Yanjun Zhang, Sergey Salikhov, Christopher R. W. Edwards, Natalia L. Klyachko, Yasufumi Takahashi, Alexander G. Majouga, Yuri E. Korchev, Alexander S. Erofeev
Summary: This paper introduces a technique for the detection of chemotherapy drugs using platinized carbon nanoelectrodes. Through studies on various biological models, it is found that this technique has the potential to be applied in advanced tumor models.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Binbin Chu, Ajun Wang, Liang Cheng, Runzhi Chen, Huayi Shi, Bin Song, Fenglin Dong, Houyu Wang, Yao He
Summary: The fluorescence detection and imaging of ATP in living cells, body fluids, and mouse tumor models can be achieved through a newly developed fluorescent ATP nanoprobes. These nanoprobes demonstrate high sensitivity and a wide dynamic range, providing a powerful tool for the diagnosis and treatment of diseases related to abnormal fluctuation of ATP concentration.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Shulong Wang, Liangliang Zhang, Yanni Luo, Yulong Bai, Yong Huang, Shulin Zhao
Summary: Drug toxicity is a long-term concern in medical research, especially when it causes liver injury. However, improving the diagnosis and treatment of drug-induced liver injury remains a major challenge. This study presents a new dual-mode nanoprobe that can be used for both circular dichroism and photoacoustic imaging to monitor drug-induced liver injury.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Changyong Gao, Yong Wang, Zihan Ye, Zhihua Lin, Xing Ma, Qiang He
Summary: Self-propelled micro- and nanomotors have great potential in biomedical applications, but challenges such as overcoming biological barriers and in vivo navigation still need to be addressed. With continued attention and innovation, it is believed that they will improve targeted drug delivery efficiency in the future.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Analytical
Kexin Zhang, Yongchen Dai, Qiuyan Li, Yingying Su, Yi Lv
Summary: The study successfully designed a simple unimolecular chemo-fluoro-luminescent probe for simultaneous detection of NaClO and ONOO-, demonstrating high sensitivity and selectivity for in vitro and in vivo imaging. Additionally, the potential of using CL kinetic curves for discrimination of multiplex analytes was shown.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Fei Ma, Ting-ting Wang, Longhe Jiang, Chun-yang Zhang
ANALYTICA CHIMICA ACTA
(2019)
Review
Pathology
Fei Ma, Su Jiang, Chun-yang Zhang
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2019)
Article
Chemistry, Multidisciplinary
Fei Ma, Qian Zhang, Chun-yang Zhang
Article
Biophysics
Fei Ma, Su Jiang, Chun-yang Zhang
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Meng Liu, Chen-chen Li, Xiliang Luo, Fei Ma, Chun-yang Zhang
ANALYTICAL CHEMISTRY
(2020)
Article
Biophysics
Qian Zhang, Yanxia Wu, Qinfeng Xu, Fei Ma, Chun-yang Zhang
Summary: DNA methylation is a vital epigenetic modification associated with various human diseases. Novel biosensors have been developed for sensitive detection of DNA methylation in recent years.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Ya-ru Meng, Dandan Zhang, Xiaoran Zou, Fei Ma, Qi Kang, Chun-yang Zhang
Summary: Developed a label-free and sensitive method for NAD(+) assay using a trifunctional split dumbbell probe and ligation-triggered isothermal rolling circle amplification. The method exhibits high sensitivity with a detection limit of 85.6 fM, good selectivity, and potential applications in biological research and clinical diagnosis related to NAD(+).
Review
Nanoscience & Nanotechnology
Meng Liu, Jian-Ge Qiu, Fei Ma, Chun-Yang Zhang
Summary: Single-molecule detection represents the ultimate sensitivity in measurement science, and with the integration of functional nanomaterials, novel single-molecule fluorescent nanosensors with excellent performance have been constructed.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Jinping Hu, Ya-ting Zhang, Yun Han, Fei Ma, Chen-zhong Li, Lin Cui, Chun-yang Zhang
Summary: This study demonstrates a novel nanosensor for antibody- and enzyme-free monitoring of m6A in clinical tissues. It has the potential to provide valuable insights into the physiological and pathological functions of m6A.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Su Jiang, Meng Liu, Wei Tantai, Qinfeng Xu, Xiaoran Zou, Fei Ma, Chun-yang Zhang
Summary: We have developed an aptamer-mediated rolling circle amplification approach for the label-free and sensitive detection of histone-modifying enzyme activity. This method can achieve femtomolar sensitivity for the assay of histone acetyltransferase Tip60, and can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yan Zhang, Jinping Hu, Xiaoran Zou, Fei Ma, Jian-Ge Qiu, Chun-yang Zhang
Summary: The development of a new fluorescent biosensor for sensitive DNA methylation assay integrates single-molecule detection with endo IV-assisted signal amplification. The biosensor shows good selectivity and high sensitivity with a detection limit of 7.3 x 10(-17) M, capable of distinguishing as low as 0.01% methylation level and analyzing genomic DNA methylation even in a single cancer cell.
CHEMICAL COMMUNICATIONS
(2021)
Review
Materials Science, Biomaterials
Fei Ma, Qian Zhang, Chun-yang Zhang
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Chemistry, Multidisciplinary
Lijian Zhang, Fei Ma, Ao Qi, Lulu Liu, Junjie Zhang, Simin Xu, Qisheng Zhong, Yusen Chen, Chun-yang Zhang, Chun Cai
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Meng Liu, Di Zhang, Xuechong Zhang, Qinfeng Xu, Fei Ma, Chun-yang Zhang
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Fei Ma, Meng Liu, Chun-yang Zhang
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.
