Article
Microbiology
Sinchan Banerjee, Anna Bedics, Erika Toth, Balazs Kriszt, Andre R. Soares, Karoly Boka, Andras Tancsics
Summary: Members of the genus Pseudomonas are widely distributed in hydrocarbon contaminated environments due to their ability to degrade various petroleum hydrocarbons. In this study, a novel bacterium designated as MAP12(T) was isolated, which showed efficient degradation of xylene under both aerobic and microaerobic conditions. The analysis of 16S rRNA gene sequence and genome confirmed that MAP12(T) represents a new species of the genus Pseudomonas with distinct characteristics.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Ernestina Hauptfeld, Jordi Pelkmans, Terry T. Huisman, Armin Anocic, Basten L. Snoek, F. A. Bastiaan von Meijenfeldt, Jan Gerritse, Johan van Leeuwen, Gert Leurink, Arie van Lit, Ruud van Uffelen, Margot C. Koster, Bas E. Dutilh
Summary: Biodegradation is a sustainable and cost-effective solution for groundwater pollution. In this study, the microbial populations involved in the biodegradation of poly-contaminants in a heavily contaminated groundwater pipeline were investigated. The microbial communities were analyzed using genome-resolved metagenomic analysis, revealing the changing microbial communities in a highly effective groundwater treatment system.
Article
Engineering, Environmental
Ernestina Hauptfeld, Jordi Pelkmans, Terry T. Huisman, Armin Anocic, Basten L. Snoek, F. A. Bastiaan von Meijenfeldt, Jan Gerritse, Johan van Leeuwen, Gert Leurink, Arie van Lit, Ruud van Uffelen, Margot C. Koster, Bas E. Dutilh
Summary: This study investigates the microbial communities involved in the biodegradation of poly-contaminants in a groundwater treatment system. The genome-resolved metagenomic analysis revealed significant differences in microbial populations between the plant and the contaminated park groundwater. The findings provide valuable insights into the efficacy of microbial biodegradation in treating complex groundwater contaminants.
Article
Environmental Sciences
Marleen Schuebl, Aleksandra Kiecak, Katrin Hug, Jutta Lintelmann, Ralf Zimmermann, Christine Stumpp
Summary: Pharmaceutically active compounds have been detected in groundwater worldwide, but their fate in the environment has not been thoroughly investigated. This study characterized the transport behavior of five selected compounds in different sediments, showing that pharmaceutical transport is influenced by sediment type and redox conditions.
JOURNAL OF CONTAMINANT HYDROLOGY
(2021)
Article
Environmental Sciences
Xuexia Chen, Shuai Zhang, Lijin Yi, Zhengwei Liu, Xiangyu Ye, Bo Yu, Shuai Shi, Xiaoxia Lu
Summary: The contamination of BTEX in the soil and groundwater of petrochemical sites near the Yangtze River poses a threat to ecosystems and human health. This study evaluated the biodegradation of BTEX in the subsurface environment, providing scientific basis for bioremediation. Molecular analysis of field samples and laboratory microcosm studies were conducted. The results showed that certain bacteria were dominant in highly or slightly contaminated areas, and the relative abundances of predicted functions were higher in the highly contaminated samples. The biodegradation of BTEX occurred in microcosms with different electron acceptors, and the rate constants varied.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Gurpreet Kaur, Joanna Lecka, Magdalena Krol, Satinder Kaur Brar
Summary: This study examined the feasibility of using geothermal heating to enhance the bioremediation of BTEX pollutants. Bacterial strains with high degradation ability were isolated from soil at different depths and identified as novel BTEX-degrading strains, except for Bacillus subtilis. Results showed that higher temperatures can stimulate biodegradation, suggesting that geothermal heating could be a potential stimulant for in-situ bioremediation.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Yufei Shi, Guochen Shen, Jinju Geng, Yingying Fu, Shengnan Li, Gang Wu, Liye Wang, Ke Xu, Hongqiang Ren
Summary: Pharmaceutically active compounds (PhACs) such as carbamazepine (CBZ), erythromycin (ERY), atenolol (ATL), and clofibric acid (CA) are frequently detected in aquatic environments, raising concerns due to their persistence and ecological risk. The UV/H2O2 advanced oxidation process is effective for removing pharmaceuticals from wastewater. Different degradation rates were observed for the four PhACs studied, and a kinetic model combining steady state concentrations successfully predicted the degradation process of pharmaceuticals.
Article
Environmental Sciences
Yang Yang, Juan Li, Ningqing Lv, Haizhou Wang, Hao Zhang
Summary: Light non-aqueous phase liquids (LNAPL) pose a risk due to their multiple chemical ingredients that can release dissolved and vapor-phase plumes. The migration and transformation of benzene, toluene, ethylbenzene, and o-xylene (BTEX) in petrochemical polluted sites are influenced by groundwater table fluctuation (GTF). Using the TMVOC model, the BTEX multiphase migration and transformation pattern was simulated under stable and fluctuating groundwater table conditions, showing increased pollution depth, area, and mass under GTF. GTF promotes the conversion of NAPL-phase pollutants to water pollutants, and impacts the transport flux and range of gaseous pollutants at the atmospheric boundary.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Multidisciplinary
Necati Menek, Ceren Ugurlar, Okan Ucarh, Yeliz Karaman, Sasha Omanovic, Saloumeh Ghasemian
Summary: The degradation of Calmagite in H2O2/UV/US, H2O2/US, H2O2, and US processes were investigated and optimized, achieving over 98% degradation in 20-60 minutes. The proposed degradation pathways were established from (OH)-O-center dot mediated degradation of the azo dye, with first-order kinetics fitting the degradation best.
ALEXANDRIA ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Amin Mojiri, John L. Zhou, Harsha Ratnaweera, Shahabaldin Rezania, Mansoureh Nazari
Summary: This study focuses on the occurrence of PPCPs in natural water bodies globally and examines the impact of various factors on the fluxes of pollutants into receiving waterways. High concentrations of PPCPs in water bodies have harmful effects on aquatic organisms, while algae-based systems are found to be effective in removing certain PPCPs.
Article
Environmental Sciences
Marton Papai, Tibor Benedek, Andras Tancsics, Till L. V. Bornemann, Julia Plewka, Alexander J. Probst, Daood Hussein, Gergely Maroti, Ofir Menashe, Balazs Kriszt
Summary: Diclofenac, ibuprofen, and carbamazepine are commonly found pharmaceutical residues in aquatic ecosystems. This study found bacteria that may be involved in their degradation and their genomes were reconstructed and annotated. The results showed the potential involvement of certain bacterial genera in the biodegradation of these pharmaceutical compounds. Some bacterial isolates were able to eliminate diclofenac and ibuprofen with a concentration reduction of over 90%.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Dong-Jin Son, Chang-Soo Kim, Jun-Won Park, Jae-Ho Lee, Soo-Hyung Lee, Sun-Kyoung Shin, Dong-Hwan Jeong
Summary: Overconsumption of pharmaceuticals is causing negative impacts on human and wildlife endocrine systems. Previous studies have investigated the occurrence and fate of pharmaceuticals in municipal wastewater treatment plants (WWTPs), but the understanding of their removal mechanism in both liquid and solid phases is limited. This study assessed pharmaceuticals in both liquid and solid phases in three municipal WWTPs with different treatment configurations, providing valuable information for the evaluation of the fate of pharmaceuticals in biological processes of WWTPs.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Genetics & Heredity
Michael O. Eze
Summary: This study successfully isolated a bacterial consortium capable of degrading diesel fuel hydrocarbons through successive enrichment of microorganisms, revealing the specific roles of different microbial populations and metabolic pathways in the degradation of BTEX. Metagenome analysis identified 105 putative coding DNA sequences responsible for BTEX activation and central metabolism, indicating the potential of the consortium for environmental remediation of petroleum hydrocarbons with approximately 70% biodegradation efficiency achieved in contaminated soils.
Article
Environmental Sciences
T. Sohrabi, M. Shakiba, F. Mirzaei, A. A. Pourbabaee
Summary: This study investigates the ability of Bacillus sp. strain PS to degrade BTEX under saline and hypoxic conditions. The results show that the initial concentration of BTEX and nitrate are the most influential factors in BTEX biodegradation.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hui-jun Wu, Xian-yuan Du, Wen-jing Wu, Jin Zheng, Jia-yu Song, Jia-cai Xie
Summary: Petroleum hydrocarbon contamination is a major concern for the environment and public health. In this study, a microbial consortium capable of degrading petroleum hydrocarbons was enriched using crude oil. 16S rRNA gene analysis revealed the structural characteristics of this community, while metagenomic analysis identified the specific microbial organisms and metabolic pathways involved in the degradation of cyclohexane and BTEX components. The results demonstrated the synergistic interactions between different bacterial genera during the hydrocarbon degradation process.
Article
Environmental Sciences
Jaroslav Mosko, Michael Pohorely, Tomas Cajthaml, Michal Jeremias, Ana A. Robles-Aguilar, Siarhei Skoblia, Zdenek Beno, Petra Innemanova, Lucie Linhartova, Klara Michalikova, Erik Meers
Summary: The study focused on using slow pyrolysis to treat organic pollutants in stabilized sewage sludge, finding that temperatures above 600 degrees Celsius effectively removed the majority of organic pollutants. It was proposed that high temperature (>600 degrees Celsius) slow pyrolysis can satisfactorily remove organic pollutants from resulting sludge-char, making it a safe soil improver.
Article
Pharmacology & Pharmacy
Kamila Sredlova, Simona Silhavecka, Lucie Linhartova, Jaroslav Semerad, Klara Michalikova, Martin Pivokonsky, Tomas Cajthaml
Summary: Secondary metabolites of cyanobacteria and algae released during algal blooms can have toxic effects, with microcystins being a common concern. This study aimed to compare the sensitivity of different ecotoxicological assays towards cellular algal organic matter (COM) from the toxin-producing cyanobacterium Microcystis aeruginosa. Multiple endpoints were investigated, and results showed toxicity to certain cell lines and organisms, indicating the presence of unknown factors contributing to the toxicity of M. aeruginosa.
Article
Environmental Sciences
Jaroslav Semerad, Alena Sevcu, Nhung H. A. Nguyen, Pavel Hrabak, Roman Spanek, Katerina Bobcikova, Kristyna Pospiskova, Jan Filip, Ivo Medrik, Josef Kaslik, Ivo Safarik, Alena Filipova, Jaroslav Nosek, Martin Pivokonsky, Tomas Cajthaml
Summary: The study investigated the remediation of chlorinated ethenes in groundwater using biochar-based composites containing nanoscale zero-valent iron (nZVI/BC) and natural resident microbes, supported by whey addition. The materials were able to decrease the original TCE concentration by 99% in 30 days, with biotic and abiotic transformation mechanisms involved in the process. The addition of whey increased bacterial biomass in the groundwater samples, aiding in the transformation of CEs and formation of less chlorinated products, demonstrating the potential and environmental safety of this nanobioremediation approach.
Article
Environmental Sciences
Michal Seres, Petra Innemanova, Tereza Hnatkova, Milos Rozkosny, Alexandros Stefanakis, Jaroslav Semerad, Tomas Cajthaml
Summary: The decrease in precipitation has negatively impacted agriculture, with the reuse of treated wastewater potentially offering a solution. Experimental results in the Czech Republic demonstrate that wastewater irrigation significantly enhances crop growth, yet the associated risks of contamination require careful attention when implementing wastewater irrigation practices.
Article
Microbiology
Andrea Zubrova, Klara Michalikova, Jaroslav Semerad, Michal Strejcek, Tomas Cajthaml, Jachym Suman, Ondrej Uhlik
Summary: The bacterial aromatic ring-hydroxylating dioxygenases (ARHDs) are involved in the degradation of aromatic pollutants and natural plant-derived aromatics. This study supports the hypothesis that ARHDs participate in ecological processes mediated by secondary plant metabolites (SPMs). The involvement of biphenyl 2,3-dioxygenase (BPDO) in the metabolism of SPMs in soil bacterium Pseudomonas alcaliphila JAB1 has been demonstrated, with a wide range of SPMs inducing the bphA gene in the strain JAB1.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Eva Otyepkova, Katarina Skladanova, Martin Pykal, Barbora Blahova Prudilova, Josef Kaslik, Klara Cepe, Pavel Banas, Petr Lazar, Michal Otyepka
Summary: The study found that the adsorption of organic molecules on the hBN surface is influenced by coverage, with polar molecules exhibiting strong affinity at low coverage. Calculations revealed that the diffusion barriers of molecules on the hBN surface were low, indicating behavior similar to a 2D gas.
APPLIED SURFACE SCIENCE
(2021)
Article
Microbiology
Jachym Suman, Michal Strejcek, Andrea Zubrova, Jan Capek, Jiri Wald, Klara Michalikova, Miluse Hradilova, Kamila Sredlova, Jaroslav Semerad, Tomas Cajthaml, Ondrej Uhlik
Summary: This study assessed the diversity of bphA genes in microbial populations in legacy PCB-contaminated soil through stable isotope probing, identifying a high homology sequence variant with potential substrate specificity related properties. This BphA variant was further investigated for its involvement in the metabolism of flavonoid compounds, providing new insights into the interactions between plants, microbes, and anthropogenic compounds in soil.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Vishwas G. Chandrashekhar, Thirusangumurugan Senthamarai, Ravishankar G. Kadam, Ondrej Malina, Josef Kaslik, Radek Zboril, Manoj B. Gawande, Rajenahally Jagadeesh, Matthias Beller
Summary: Development of an iron-based catalyst for hydrogenation of nitriles, capable of promoting the conversion of various nitriles into primary amines under scalable and industrially viable conditions.
Article
Engineering, Environmental
Miroslav Brumovsky, Jana Oborna, Vesna Micic, Ondrej Malina, Josef Kaslik, Daniel Tunega, Miroslav Kolos, Thilo Hofmann, Frantisek Karlicky, Jan Filip
Summary: The article discusses the significant enhancement in reactivity and corrosion resistance of zerovalent iron nanoparticles (nZVI) through nitriding. The study demonstrates the outstanding catalytic performance of iron nitrides (FexN) in various applications and their potential as a tool for trichloroethylene (TCE) dechlorination. The research findings provide valuable insights into the application of nitriding nanoparticles in environmental remediation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Jaroslav Semerad, Petra Horka, Alena Filipova, Jaroslav Kukla, Katerina Holubova, Zuzana Musilova, Katerina Jandova, Jan Frouz, Tomas Cajthaml
Summary: This study assessed the geographical trends of fish contamination by per- and polyfluorinated alkyl substances (PFASs) in the Czech section of the Elbe River and its tributary. Long chain PFASs were found in higher concentrations, while short-chain PFASs and Gen-X were detected in lower quantities. The study also revealed that PFAS concentrations increased downstream and were positively correlated with δ N-15 and negatively correlated with δ C-13. Additionally, molecular mass and the number of fluorine substituents were found to play crucial roles in PFAS bioaccumulation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Jana Steinova, Hakon Holien, Alica Kosuthova, Pavel Skaloud
Summary: This study reports a case of a three-species complex of red-fruited Cladonia lichens where mycobiont markers failed to separate the species, but photobiont identity corresponded better with lichen phenotype. This could either be an unusual case of lichen photomorphs or an example of recent speciation, where phenotypic differentiation precedes the separation of molecular markers. The authors hypothesize that association with different photobionts, possibly related to habitat differentiation, may have triggered speciation in the mycobiont species.
Article
Environmental Sciences
Petra Najmanova, Jana Steinova, Marie Czinnerova, Jan Nemecek, Roman Spanek, Vladislav Knytl, Martin Halecky
Summary: In this study, the degradation of TCE was investigated using various methods, including monitoring changes in dechlorinating bacteria, observing functional genes and specific bacteria, and analyzing the effects of elevated temperature and whey addition. The results showed that elevated temperature and whey addition significantly enhanced TCE dechlorination. This study demonstrates the feasibility and effectiveness of biological dechlorination of TCE with elevated temperature and whey addition.
Article
Engineering, Environmental
Jaroslav Semerad, Ondrej Lhotsky, Alena Filipova, Ondrej Urban, Katerina Sirova, Jana Bohackova, Michael Komarek, Tomas Cajthaml
Summary: In this study, a sequence of anoxic/oxic chemico-biological treatments was used to successfully degrade HCHs in extremely contaminated soil. The best results were achieved with mZVI/nZVI, grass cuttings, and oxygen-releasing compounds, with 80% removal of HCHs achieved in 129 days and 98% degradation achieved after 1106 days. Switching between aerobic and anaerobic phases, along with the addition of grass, created suitable conditions for the biodegradation of HCHs and monochlorobenzene/benzene by microbes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Ivana Cernajova, Jana Steinova, Zuzana Skvorova, Pavel Skaloud
Summary: Cladonia luteoalba's chemotype coincides with that of the associated Cladonia thalli, but our data did not support its distinction as a separate species. Therefore, the true nature of C. luteoalba remains unclear.
Article
Chemistry, Analytical
Tereza Cerna, Klara Michalikova, Jaroslav Semerad, Tomas Cajthaml
Summary: The study compared two analytical methods, LC-MS/MS and GC-MS/MS, for detecting 19 estrogenic EDCs in wastewater treatment plant effluents. LC-MS/MS showed lower detection limits, better stability, and met the requirements of EU regulations.
ENVIRONMENTAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.
