Article
Multidisciplinary Sciences
Ruifang Han, Juan Gao, Liming Wang, Peng Hao, Xi Chen, Yuchuan Wang, Zhixin Jiang, Li Jiang, Ting Wang, Lin Zhu, Xuan Li
Summary: MiR-146a plays a role in regulating corneal inflammation in dry eye disease and negatively regulates inflammation in human corneal epithelial cells through the IRAK1/TRAF6/NF-κB pathway. This study provides a potential therapeutic approach for the treatment of dry eye disease.
SCIENTIFIC REPORTS
(2023)
Review
Medicine, General & Internal
Weiming Tang, Yusha Tao, Emmanuel Fajardo, Elena Ivanova Reipold, Roger Chou, Joseph D. Tucker, Philippa Easterbrook
Summary: This study evaluated the diagnostic performance of point-of-care (PoC) HCV viral load assays and found that they demonstrate excellent performance in various settings and populations. There was a slight reduction in sensitivity for fingerstick samples compared to serum or plasma, but the specificity was similar between frozen and fresh samples.
Article
Medicine, Research & Experimental
Liang He, Zhuoran Wang, Rui Zhou, Wei Xiong, Yuqiao Yang, Ning Song, Jinqiao Qian
Summary: Myocardial ischemia/reperfusion (I/R) injury is a common cause of mortality. Cardiac miR-146a is emerging as a potent regulator of myocardial function. Dexmedetomidine preconditioning provides cardioprotective effects, of which mechanisms related to miR-146a-3p targeting IRAK1 and TRAF6 through inhibition of NF-kappa B signaling pathway and ROS emission.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Xinying Zhou, Zelin Zhang, Hui Xu, Bo Zhu, Lijie Zhang, Linmiao Lie, Yingqi Huang, Xialin Du, Honglin Liu, Yanfen Li, Yulan Huang, Shengfeng Hu, Chaoying Zhou, Qian Wen, Mailkel P. Pepplenbosch, Li Ma
Summary: Viperin protein is increased in abundance in patients with lymphatic and pulmonary tuberculosis (TB), and its deficiency reduces mycobacterium tuberculosis bacterial loads and enhances macrophage responses. Viperin promotes Mtb infection by inhibiting host innate immune responses in macrophages.
Article
Biochemistry & Molecular Biology
Suyun Jeong, Young-seok Lee, Kiyoon Kim, Ji-su Yoon, Sungsoo Kim, Joohun Ha, Insug Kang, Wonchae Choe
Summary: The compound 2-O-methylhonokiol was found to inhibit HCV replication by activating the host immune response pathway. This effect was achieved through the upregulation of innate immune responses and the suppression of autophagy caused by HCV. The results indicate that 2-O-methylhonokiol enhances the host immune response and suppresses HCV replication via TRAF6-mediated NF-kB activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Peifeng Han, Keisuke Sunada-Nara, Nobuyuki Kawashima, Mayuko Fujii, Shihan Wang, Thoai Quoc Kieu, Ziniu Yu, Takashi Okiji
Summary: This study found that miR-146b-5p can suppress inflammation in human dental pulp cells stimulated by LPS, and its synthesis is controlled via the NF-κB/IL6/STAT3 signaling cascade. The study also found that miR-146b-5p down-regulates the expression of pro-inflammatory mediators by targeting TRAF6, IRAK1, and RELA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Maximilian Bunz, Michael Ritter, Michael Schindler
Summary: This article summarizes the current knowledge related to the export pathway of hepatitis C virus (HCV) particles. In addition to the prototypical anterograde transport, possible routes of HCV release include ER-to-endosomal transport, secretory autophagy, and poorly described mechanisms of unconventional protein secretion.
TRENDS IN MICROBIOLOGY
(2022)
Article
Oncology
Rongqi Wang, Suxian Zhao, Jinghua Du, Jie Qiao, Wencong Li, Yuemin Nan
Summary: The study revealed a significant decrease in serum hepcidin levels in HCV-infected patients, indicating a potential iron metabolism disorder. Evaluating patient conditions by measuring hepcidin levels and regulating iron metabolism may improve prognosis for HCV patients.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Virology
Jianguo Yan, Yuting Zhang, Yan Su, Linlin Tian, Peifang Qin, Xingfeng Xu, Yali Zhou
Summary: Hepatitis C virus can evade immune surveillance by activating miR-125a and suppressing interferon signaling pathway, allowing the virus to persist in hosts. Targeting this pathway may offer an efficient approach to treating HCV and boosting antiviral immune responses in infected patients.
Article
Cell Biology
Zhongfei Zhang, Xiaoxiong Zou, Run Zhang, Yu Xie, Zhiming Feng, Feng Li, Jianbang Han, Haitao Sun, Qian Ouyang, Shiting Hua, Bingke Lv, Tian Hua, Zhizheng Liu, Yingqian Cai, Yuxi Zou, Yanping Tang, Xiaodan Jiang
Summary: The study showed that hUMSC-Exos can attenuate microglia-mediated neuroinflammation and improve neural deficits following ischemic stroke. MiR-146a-5p plays a crucial role in this process.
Article
Medicine, Research & Experimental
Guang Yang, Yuan Zhao
Summary: The study demonstrated that miR-490-3p overexpression significantly inhibited LPS-induced ALI and inflammatory responses by targeting and attenuating IRAK1 expression, thus inactivating the LPS-mediated TRAF6/NF-kappa B pathway. MiR-490-3p was found to be downregulated in the LPS-induced ALI model, while pro-inflammatory factors and IRAK1 were upregulated, negatively correlated with miR-490-3p expression. Overexpression of miR-490-3p inhibited lung epithelial cell injury and inflammatory response.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Medicine, Research & Experimental
Thomas Hoyler, Bettina Bannert, Cedric Andre, Damian Beck, Thomas Boulay, David Buffet, Nadja Caesar, Thomas Calzascia, Janet Dawson, Diego Kyburz, Robert Hennze, Christine Huppertz, Amanda Littlewood-Evans, Pius Loetscher, Kirsten D. Mertz, Satoru Niwa, Gautier Robert, James S. Rush, Giulia Ruzzante, Sophie Sarret, Thomas Stein, Ismahane Touil, Grazyna Wieczorek, Geraldine Zipfel, Stuart Hawtin, Tobias Junt
Summary: IL-1 receptor-activated kinase 1 (IRAK1) plays a significant role in joint inflammation and neutrophil chemoattraction. Targeting IRAK1 may be a potential option for treating inflammatory arthritis.
Article
Microbiology
Jakob Tschaepe, Anika Cobernuss-Rahn, Sean Boyle, Neil Parkin, Ben LaBrot, Shagufta Aslam, Stephen Young, Peter Gohl
Summary: The cobas 5800 system is a new nucleic acid testing system that can perform qualitative and quantitative testing, including viral load determination. The system has been compared to the cobas 6800 system and found to have equivalent performance. This study demonstrates that the cobas 5800 system is a viable option for low- to mid-throughput laboratories looking to optimize their viral molecular testing efficiency.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Medicinal
Liqiang Fu, Jing Zhang, Bin Shen, Linglong Kong, Yingtao Liu, Wangyang Tu, Wenqian Wang, Xin Cai, Xiaotao Wang, Na Cheng, Mingxuan Xia, Tianyuan Zhou, Qian Liu, Yanping Xu, Jennifer Yang, Paul Gavine, Ulrike Philippar, Ricardo Attar, James P. Edwards, Jennifer D. Venable, Xuedong Dai
Summary: The study discusses the potent anti-proliferative effects of IRAK1 degraders on ABC DLBCL cells with MyD88 mutation, as well as their inhibitory effects on IRAK1 downstream signaling pathways, indicating the potential of these IRAK1 degraders for treating cancers dependent on IRAK1 scaffolding function.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Yucel Aydin, Ali Riza Koksal, Venu Reddy, Dong Lin, Hanadi Osman, Zahra Heidari, Sadeq Mutlab Rhadhi, William C. Wimley, Mansour A. Parsi, Srikanta Dash
Summary: This study elucidates the impact of impaired autophagic degradation and blocking extracellular vesicle release on persistent HCV infection, revealing potential antiviral strategies. Inhibition of extracellular vesicle release severely suppresses virus replication without affecting host cell viability, and triggers interferon lambda 1 secretion, suggesting it as an innate immune escape mechanism promoting persistent HCV infection.
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.