Article
Oncology
Marco Silvestri, Carolina Reduzzi, Giancarlo Feliciello, Marta Vismara, Thomas Schamberger, Caecilia Koestler, Rosita Motta, Stefano Calza, Cristina Ferraris, Andrea Vingiani, Giancarlo Pruneri, Maria Grazia Daidone, Christoph A. Klein, Bernhard Polzer, Vera Cappelletti
Summary: CTMs represent cancer cells detached from solid tumors and can provide insights into metastatic mechanisms. Analyzing CNAs in CTMs, which often contain unknown fractions of leukocytes, presents challenges. By titrating known numbers of leukocytes into cancer cells, we generated tumor fractions ranging from 0-100%. Through low-pass sequencing and the use of ichorCNA algorithm, we accurately predicted the tumor fraction of CTMs isolated from early-stage breast cancer patients. These CTMs were found to contain cancer cells with a range of 8-48%, with notable differences in CNAs compared to matched primary tumors, suggesting potential implications for metastasis-related genes that should be further explored.
Article
Biochemistry & Molecular Biology
Jacqueline Aparecida Torres, Angelo Borsarelli Carvalho Brito, Virgilio Souza e Silva, Iara Monique Messias, Alexcia Camila Braun, Anna Paula Carreta Ruano, Marcilei E. C. Buim, Dirce Maria Carraro, Ludmilla Thome Domingos Chinen
Summary: Circulating tumor cells (CTCs) and/or circulating tumor microemboli (CTM) from non-small cell lung cancer (NSCLC) patients could serve as a non-invasive tool for prognosis, acting as liquid biopsy. This study found that CTCs/CTMs from NSCLC patients expressed immune evasion markers TGF beta RI/CD47, and the presence of CTMs CD47+ was associated with poor progression-free survival (PFS).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Wojciech Jelski, Barbara Mroczko
Summary: Gastric cancer is a highly malignant tumor with poor prognosis, and early diagnosis and effective monitoring are crucial for reducing disease burden and mortality. Blood-based biomarker assays have the potential for early-stage detection of gastric cancer. However, the currently used tumor marker assays have limitations in sensitivity and specificity. In recent years, multiple markers have been identified and tested for their clinical relevance in gastric cancer management. This review summarizes the available literature on various blood-based biomarkers and discusses their potential for early diagnosis and accurate prediction of therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Miao Jiang, Shuiling Jin, Jinming Han, Tong Li, Jianxiang Shi, Qian Zhong, Wen Li, Wenxue Tang, Qinqin Huang, Hong Zong
Summary: Liquid biopsy is a promising minimally invasive method for early colorectal cancer detection, sampling circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA (miRNA) and extracellular vesicles (EVs) to provide information on tumor dynamics.
BIOMARKER RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Can Li, Wei He, Nan Wang, Zhipeng Xi, Rongrong Deng, Xiyu Liu, Ran Kang, Lin Xie, Xin Liu
Summary: This paper reviews the recent research progress of microfluidic technology in CTCs separation and detection, discusses the potential application of microfluidic technology in the treatment of tumor metastasis, and briefly explores the development prospect of microfluidic biosensors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, Research & Experimental
Qian Deng, Bo Jiang, Haijiao Yan, Jun Wu, Zhenzhen Cao
Summary: Circulating tumor cells (CTCs) are shed from primary tumor or metastatic sites into the bloodstream and subsequently seed into distant tissues, serving as precursors of metastases. Gastric cancer (GC) is highly heterogeneous, making secondary pathological biopsy difficult and invasive examination harmful. In recent years, CTCs have shown great potential for tumor diagnosis, prognosis prediction, efficacy detection, and treatment guidance, although their role in GC remains limited. This review focuses on landmark studies demonstrating the technical approaches of CTCs monitoring in GC and highlights the clinical application of CTCs numbers and phenotypes in monitoring therapeutic efficacy and predicting patient prognosis through sequential blood analyses.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Review
Oncology
Zuxi Li, Meijuan Song, Shangjun Han, Chuanwei Jin, Jing Yang
Summary: Circulating tumor cells (CTC) are associated with the prognosis of patients with gastric cancer. Gastric cancer patients with positive CTC may have a poorer prognosis compared to those with CTC-negative tumors. CTC-positive patients have more advanced TNM staging, poorer tumor differentiation, and earlier distant metastasis.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jozsef Petrik, Donatella Verbanac, Marija Fabijanec, Andrea Hulina-Tomaskovic, Andrea Ceri, Anita Somborac-Bacura, Roberta Petlevski, Marija Grdic Rajkovic, Lada Rumora, Bozo Kruslin, Mario Stefanovic, Neven Ljubicic, Neven Barsic, Antonija Hanzek, Luka Bockor, Ivana Celap, Alma Demirovic, Karmela Barisic
Summary: This review provides an overview of the research conducted on circulating tumor cells (CTCs) with the aim of finding suitable detection systems and assessing the possibility of clinical applications in patients with colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Hsueh-Yao Chu, Chih-Yung Yang, Ping-Hao Yeh, Chun-Jieh Hsu, Lu-Wei Chang, Wei-Jen Chan, Chien-Ping Lin, You-You Lyu, Wei-Cheng Wu, Chun-Wei Lee, Jen-Kuei Wu, Jeng-Kai Jiang, Fan-Gang Tseng
Summary: CTCs can be used for predicting recurrence in CRC patients. CTC clusters/microemboli have higher correlation with metastasis, and when both the number of CTCs and serum CEA levels are high, the prognosis in CRC patients may be worse.
Article
Engineering, Multidisciplinary
Hsien- Lin, Ying-Chih Chang
Summary: A semiautomated method proposed in this study predicts CRC based on counting CTCs, using features identified by SVM. The wrapping degree of cytoplasm around the nucleus is introduced to improve results. Semiautomated identification was found to be nearly as accurate and much faster than manual identification for detecting CRC.
AIN SHAMS ENGINEERING JOURNAL
(2021)
Article
Medicine, General & Internal
Joon Hyung Jhi, Gwang Ha Kim, Su Jin Park, Dong Uk Kim, Moon Won Lee, Bong Eun Lee, Chae Hwa Kwon, Yoon-Kyoung Cho
Summary: This study found that high levels of CTCs and TWIST (+) CTCs in patients with metastatic gastric cancer are associated with worse overall survival.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Oncology
Yu-Ping Yang, Teresa M. Giret, Richard J. Cote
Summary: Circulating tumor cells (CTCs) are rare in blood, but play a crucial role in metastasis and have potential as a biomarker for cancer diagnosis, prognosis, and treatment monitoring. Advancements in CTC isolation techniques have improved detection sensitivity and accuracy, leading to increased focus on molecular characterization and understanding interactions between CTCs and associated cells for precision medicine. Challenges and opportunities in clinical utilization of CTCs are being addressed for future advancements in cancer research.
Article
Oncology
Yuanrui Liu, Rong Zhao, Zaichun Xie, Zhiyu Pang, Shengjie Chen, Qian Xu, Zhanfeng Zhang
Summary: In this study, circulating tumor cells (CTCs) were detected in the peripheral blood of tumor patients, and their numbers and subtypes were found to be related to different tumor diseases and stages. CTCs with mesenchymal phenotypes were closely associated with lymph node or vascular metastasis. CTC detection can aid in early diagnosis of tumor diseases and monitoring of disease progression.
Review
Materials Science, Multidisciplinary
Kutay Sagdic, Fatih Inci
Summary: This review discusses the broad applications of smart material-integrated systems in isolating and profiling rare circulating tumor cells and circulating tumor microemboli, highlighting the importance of advanced techniques in exploring tumor evolution, heterogeneity, and metastatic characteristics. It also emphasizes the challenges of tracking and isolating rare cells using conventional methods, and the significant impact of smart material-based technologies in cell detection and isolation for downstream analyses.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Biophysics
Syed Harris Hussain, Cesar S. Huertas, Arnan Mitchell, Anne-Laure Deman, Emmanuelle Laurenceau
Summary: Analysis of circulating tumor cells (CTCs) and their associated biomarkers can provide valuable information for lung and prostate cancer, aiding in early detection and personalized treatment selection. Biosensors offer an elegant alternative to conventional methods for real-time, multiplexed analysis of cancer biomarkers at lower concentrations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Oncology
Xiaofan Pu, Chaolei Zhang, Guoping Ding, Hongpeng Gu, Yang Lv, Tao Shen, Tianshu Pang, Liping Cao, Shengnan Jia
Summary: This study demonstrated the potential utility of the sEV-miRNA d-signature in the diagnosis of PDAC via machine learning methods. A novel sEV biomarker, miR-664a-3p, was identified for the diagnosis of PDAC. It can also potentially promote angiogenesis and metastasis, provide insight into PDAC pathogenesis, and reveal novel regulators of this disease.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Jiaping Wang, Zhijuan Xu, Yanli Lai, Yanli Zhang, Ping Zhang, Qitian Mu, Shujun Yang, Yongcheng Sun, Lixia Sheng, Guifang Ouyang
Summary: This study demonstrates the significance of PD-1 in EBV-infected lymphoma cells. Silencing PD-1 enhances the tumor targeting effect of EBV-specific killer T cells on B lymphocytes and attenuates the immune escape effect.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Qiliang Peng, Jialong Tao, Yingjie Xu, Yi Shen, Yong Wang, Yang Jiao, Yiheng Mao, Yaqun Zhu, Yulong Liu, Ye Tian
Summary: This study investigates the potential role of lipid metabolism-associated genes (LMAGs) in neoadjuvant chemoradiotherapy (nCRT) and immunotherapy for rectal cancer. The results suggest that the SREBF2 gene is a highly predictive factor for nCRT in rectal cancer and is associated with favorable prognosis. SREBF2 is also closely associated with immune cell infiltration and immunotherapy-related genes.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Shiquan Li, Nan Zhang, Yongping Yang, Tongjun Liu
Summary: This study investigated the potential molecular mechanism of SPDEF in immune evasion of colorectal cancer (CRC) and found that it suppresses immune evasion by activating CCL28 through the modulation of M2 polarization of macrophages. These findings provide a new research direction and potential therapeutic target for immunotherapy in CRC.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Manas Sehgal, Soundharya Ramu, Joel Markus Vaz, Yogheshwer Raja Ganapathy, Srinath Muralidharan, Sankalpa Venkatraghavan, Mohit Kumar Jolly
Summary: This study investigates the relationship between gene expression patterns and phenotypic plasticity and heterogeneity in colorectal cancer (CRC). The results demonstrate the interconnectedness between different Consensus Molecular Subtypes (CMS) of CRC and specific phenotypes such as epithelial and mesenchymal characteristics. Additionally, the study reveals correlations between metabolic pathways and phenotypic scores, as well as between PD-L1 activity and mesenchymal phenotype. Single-cell RNA sequencing analysis further confirms the heterogeneity of different CMS subtypes. These findings have important implications for understanding CRC heterogeneity and developing targeted therapies.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Yutong Zou, Siyao Guo, Yan Liao, Weidong Chen, Ziyun Chen, Junkai Chen, Lili Wen, Xianbiao Xie
Summary: This study found that ceramide metabolism is associated with the progression and clinical outcome of osteosarcoma by analyzing data from osteosarcoma patients. The gene ST3GAL1 plays an important role in osteosarcoma, regulating the tumor immune microenvironment and affecting T cell function. It may become a new target for the treatment of osteosarcoma.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Chuanhui Chen, Mengzhi Wan, Xiong Peng, Qing Zhang, Yu Liu
Summary: This study examines the function and mechanism of the ceRNA network centered around GPR37 in LUAD. The findings show that high expression of GPR37 in LUAD tissue samples is associated with poor prognosis, and it may regulate the expression of downstream target genes by competitively binding to lncRNA DLEU1 and miR-4458.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Junping Li, Hong Hu, Jinping He, Yuling Hu, Manting Liu, Bihui Cao, Dongni Chen, Xiaodie Ye, Jian Zhang, Zhiru Zhang, Wen Long, Hui Lian, Deji Chen, Likun Chen, Lili Yang, Zhenfeng Zhang
Summary: Sequential administration of CDC7 inhibitor XL413 after carboplatin enhances the chemotherapeutic effect of carboplatin on ovarian cancer cells, possibly by inhibiting homologous recombination repair activity and increasing the accumulation of chemotherapy-induced DNA damage.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Madison Catalanotto, Joel Markus Vaz, Camille Abshire, Reneau Youngblood, Min Chu, Herbert Levine, Mohit Kumar Jolly, Ana -Maria Dragoi
Summary: The study demonstrates that loss of FLASH in cancer cells leads to a hybrid E/M phenotype with high epithelial scores, suggesting FLASH acts as a repressor of the epithelial phenotype. Additionally, FLASH expression is inversely correlated with the epithelial score and subsets of mesenchymal markers are distinctly up-regulated in FLASH, NPAT, or SLBP-depleted cells.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Xiaorui Wang, Na Li, Minying Zheng, Yongjun Yu, Shiwu Zhang
Summary: Adipocytes are derived from pluripotent mesenchymal stem cells and histone modifications play a key role in their differentiation. Recent studies have shown that cancer stem cells can differentiate into adipocytes, reducing the malignancy of cancer cells.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Hana Q. Sadida, Alanoud Abdulla, Sara Al Marzooqi, Sheema Hashem, Muzafar A. Macha, Ammira S. Al-Shabeeb Akil, Ajaz A. Bhat
Summary: Cancer heterogeneity and drug resistance are major obstacles to effective cancer treatment, and epigenetic modifications play a pivotal role in these processes. This review explores essential epigenetic modifications, including DNA methylation, histone modifications, and chromatin remodeling, and discusses their complex contributions to cancer biology. However, the interplay of epigenetic and genetic changes in cancer cells presents unique challenges that must be addressed to fully exploit the potential of epigenetic modifications.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Pedro De Marchi, Leticia Ferro Leal, Luciane Sussuchi da Silva, Rodrigo de Oliveira Cavagna, Flavio Augusto Ferreira da Silva, Vinicius Duval da Silva, Eduardo C. A. da Silva, Augusto O. Saito, Vladmir C. Cordeiro de Lima, Rui Manuel Reis
Summary: The TIS and IFN-gamma signatures are predictive biomarkers for identifying NSCLC patients who could potentially benefit from immune checkpoint inhibitor therapies.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Giovanni Marchi, Anna Rajavuori, Mai T. N. Nguyen, Kaisa Huhtinen, Sinikka Oksa, Sakari Hietanen, Sampsa Hautaniemi, Johanna Hynninen, Jaana Oikkonen
Summary: The study shows that ctDNA can adequately represent high-grade serous ovarian carcinoma (HGSC), and the mutations observed at relapse suggest personalized therapy options.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Yuncang Yuan, Jiawei Fan, Dandan Liang, Shijie Wang, Xu Luo, Yongjie Zhu, Nan Liu, Tingxiu Xiang, Xudong Zhao
Summary: This study demonstrates that csGRP78-directed CAR-T cells can selectively kill pancreatic cancer cells, and the combination with chemotherapy enhances cytotoxicity.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Niyati Piplani, Tanusri Roy, Neha Saxena, Shamik Sen
Summary: The glycocalyx, a protective barrier surrounding cells, has been found to play a role in cancer cell proliferation, survival, and metastasis. However, its function in maintaining DNA/nuclear integrity during migration through dense matrices has not been explored. This study shows that the bulkiness of the glycocalyx is inversely associated with nuclear stresses, and highlights its mechanical role in shielding migration-associated stresses.
TRANSLATIONAL ONCOLOGY
(2024)
