Article
Cell Biology
Joo-Ok Min, Hoang-Anh Ho, Wonjae Lee, Byung Chul Jung, Sung Jun Park, Seokjoong Kim, Seung-Jae Lee
Summary: Cell-to-cell propagation of protein aggregates is involved in the progression of neurodegenerative diseases. We screened a small-molecule library and identified simvastatin as a potential agent that suppresses the propagation of α-synuclein and mutant huntingtin. Simvastatin effectively improved behavioral deficits and reduced α-synuclein accumulation in a synucleinopathy model, suggesting the importance of inhibiting brain cholesterol synthesis in its effects.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Shiori Awa, Genjiro Suzuki, Masami Masuda-Suzukake, Takashi Nonaka, Minoru Saito, Masato Hasegawa
Summary: This study investigated the early phase of phosphorylated alpha-synuclein accumulation by injecting alpha-synuclein fibrils into the brains of wild-type mice. The results showed that phosphorylated alpha-synuclein appeared within a few days after injection, initially in presynaptic regions and subsequently extending its localization to axons and cell bodies.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yunxia Ma, Desiree Charlotte Schroeder, Miljana Nenkov, Maryam Noor Rizwan, Mohamed Abubrig, Juergen Sonnemann, Jose M. Murrieta-Coxca, Diana M. Morales-Prieto, Martin Westermann, Nikolaus Gassler, Yuan Chen
Summary: The study suggests that decreased EMP1 expression is significantly associated with increased tumor size in NSCLC, while overexpression of EMP2 can suppress tumor cell growth, migration, and invasion, and affect cell cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Valentina Vapore, Corrado Mazzaglia, Diego Sibilia, Mara Del Vecchio, Gernot Fruhmann, Marta Valenti, Elena Miranda, Teresa Rinaldi, Joris Winderickx, Cristina Mazzoni
Summary: The study found that expression of human wild-type NS and the polymerogenic variant G392E NS in yeast led to the formation of protein aggregates mainly localized within the endoplasmic reticulum (ER). NS inclusions delayed the exit from the lag phase, causing cellular stress, and showed higher resistance following mild oxidative stress treatments.
Review
Biochemistry & Molecular Biology
Anna Ainslie, Wouter Huiting, Lara Barazzuol, Steven Bergink
Summary: Genome instability and loss of protein homeostasis are fundamental characteristics of age-related diseases, particularly neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, and ALS. These events are interconnected and contribute to the development of neurological diseases.
Article
Biochemistry & Molecular Biology
Nima Sayyadi, Sareh Zhand, Sajad Razavi Bazaz, Majid Ebrahimi Warkiani
Summary: The study proposes a novel strategy of using Affibody functionalized microbeads (AffiBeads) for exosome detection with a high degree of efficiency. Results showed that the Affibody probe had a detection limit of 15.6 ng exosomes per mL (~12 exosomes per AffiBead), providing a basis for early-stage cancer diagnosis by sensitive detection of low expression level markers on tumor-derived exosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Hadi Yari, Maria Mikhailova, Mahsa Mardasi, Mohsen Jafarzadehgharehziaaddin, Somayeh Shahrokh, Lakshmi Thangavelu, Hosein Ahmadi, Navid Shomali, Yoda Yaghoubi, Majid Zamani, Morteza Akbari, Samira Alesaeidi
Summary: Accumulating evidence suggests that the pleiotropic effects of mesenchymal stromal cells (MSCs) are mainly mediated by the releases of soluble paracrine mediators, rather than their differentiation competencies. MSC-derived exosomes hold great potential for the treatment of neurodegeneration-associated neurological diseases due to their unique immunomodulatory and regenerative attributes. These exosomes can cross the blood-brain barrier (BBB) and efficiently deliver their cargo to damaged brain tissue, influencing various biological processes in neurons, oligodendrocytes, and astrocytes.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Alessandra Bigi, Roberta Cascella, Cristina Cecchi
Summary: The misfolding and aggregation of a-synuclein is the key feature of synucleinopathies, such as Parkinson's disease and dementia with Lewy bodies. Soluble oligomeric assemblies formed during the early stages of aggregation are toxic to neurons, while fibrillar conformers contribute to the spreading of the pathology. Recent studies have also shown that a-synuclein fibrils release soluble and highly toxic oligomeric species, leading to immediate dysfunction in recipient neurons. This review discusses the mechanisms of cellular dysfunction caused by a-synuclein oligomers and fibrils in synucleinopathies.
NEURAL REGENERATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Hyosuk Kim, Eun Hye Kim, Gijung Kwak, Sung-Gil Chi, Sun Hwa Kim, Yoosoo Yang
Summary: Exosomes are nanovesicles secreted by cells that can mediate intercellular communication and serve as promising drug delivery vehicles due to their stability, biocompatibility, and targeting abilities. They can carry therapeutic signaling molecules from specific cell types, impacting cancer treatment significantly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Zixian Yu, Shujing Lin, Fangfang Xia, Yanlei Liu, Di Zhang, Fei Wang, Yanpu Wang, Qichao Li, Jiaqi Niu, Chengxi Cao, Daxiang Cui, Nengquan Sheng, Jiazi Ren, Zhigang Wang, Di Chen
Summary: The ExoSD chip is a highly integrated platform for exosome separation and detection, capable of efficiently separating and detecting gastric cancer cell-derived exosomes with high recovery and purity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Cell Biology
Jixian Liu, Ruixing Luo, Junbin Wang, Xinyu Luan, Da Wu, Hua Chen, Qinghua Hou, Guangxian Mao, Xiaoqiang Li
Summary: The study identified that tumor cell-derived exosomal miR-770 inhibited M2 macrophage polarization via targeting MAP3K1, thus suppressing the invasion and growth of NSCLC cells. This provides a new strategy for the treatment of NSCLC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Henry Patrick Oamen, Nathaly Romero Romero, Philip Knuckles, Juha Saarikangas, Marta Radman-Livaja, Yuhong Dong, Fabrice Caudron
Summary: This study found that the natural lipid tripentadecanoin can delay the formation of protein aggregates and extend cellular lifespan, offering a potential treatment for neurodegenerative diseases. Tripentadecanoin can also protect neurons from amyloid toxicity and promote the recovery of retinal cells.
Article
Biochemistry & Molecular Biology
Merve Avar, Daniel Heinzer, Alana M. Thackray, Yingjun Liu, Marian Hruska-Plochan, Stefano Sellitto, Elke Schaper, Daniel P. Pease, Jiang-An Yin, Asvin K. K. Lakkaraju, Marc Emmenegger, Marco Losa, Andra Chincisan, Simone Hornemann, Magdalini Polymenidou, Raymond Bujdoso, Adriano Aguzzi
Summary: A genome-wide RNAi screening using the high-throughput prion measurement method QUIPPER identified 1,191 modulators of prion propagation. Among them, 20 genes were found to increase prion production when suppressed. Furthermore, a ribonucleoprotein Hnrnpk was identified as a prominent limiter of prion propagation.
Review
Clinical Neurology
Terouz Pasha, Anna Zatorska, Daulet Sharipov, Boris Rogelj, Tibor Hortobagyi, Frank Hirth
Summary: Neurodegenerative proteinopathies are characterized by abnormalities in nucleocytoplasmic transport mediated by karyopherins, which can lead to mislocalization and aggregation of disease-related proteins, potentially playing a crucial role in the onset and progression of these diseases. Targeting karyopherins pharmacologically represents a promising new avenue for disease-modifying treatments in neurodegenerative proteinopathies, as evidenced in clinical trials for other diseases like cancer and viral infections.
Article
Biochemistry & Molecular Biology
Chaoyi Chen, Hongfei Yu, Fengyan Han, Xuan Lai, Kehong Ye, Siqin Lei, Minglang Mai, Maode Lai, Honghe Zhang
Summary: The study reveals that circRHOBTB3 is upregulated in serum and downregulated in tissue samples of CRC patients, with the downregulation associated with poor prognosis. CircRHOBTB3 acts as a tumor suppressor by repressing metabolic pathways and intracellular ROS production. SNF8 is identified as the protein responsible for sorting circRHOBTB3 into exosomes, and CRC cells secrete more circRHOBTB3 compared to normal cells. Antisense oligonucleotides targeting regulatory elements of circRHOBTB3 can increase its expression and inhibit its exosomal secretion, thereby inhibiting CRC growth and metastasis.
Article
Neurosciences
Sireesha Manne, Naveen Kondru, Monica Hepker, Huajun Jin, Vellareddy Anantharam, Mechelle Lewis, Xuemei Huang, Arthi Kanthasamy, Anumantha G. Kanthasamy
JOURNAL OF NEUROIMMUNE PHARMACOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Dilshan S. Harischandra, Dharmin Rokad, Matthew L. Neal, Shivani Ghaisas, Sireesha Manne, Souvarish Sarkar, Nikhil Panicker, Gary Zenitsky, Huajun Jin, Mechelle Lewis, Xuemei Huang, Vellareddy Anantharam, Arthi Kanthasamy, Anumantha G. Kanthasamy
Article
Toxicology
Chunjuan Song, Adhithiya Charli, Jie Luo, Zainab Riaz, Huajun Jin, Vellareddy Anantharam, Arthi Kanthasamy, Anumantha G. Kanthasamy
TOXICOLOGICAL SCIENCES
(2019)
Article
Toxicology
Nyzil Massey, Sreekanth Puttachary, Sanjana Mahadev Bhat, Anumantha G. Kanthasamy, Chandrashekhar Charavaryamath
TOXICOLOGICAL SCIENCES
(2019)
Article
Immunology
Nikhil Panicker, Souvarish Sarkar, Dilshan S. Harischandra, Matthew Neal, Tae-In Kam, Huajun Jin, Hariharan Saminathan, Monica Langley, Adhithiya Charli, Manikandan Samidurai, Dharmin Rokad, Shivani Ghaisas, Olga Pletnikova, Valina L. Dawson, Ted M. Dawson, Vellareddy Anantharam, Anumantha G. Kanthasamy, Arthi Kanthasamy
JOURNAL OF EXPERIMENTAL MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Dilshan S. Harischandra, Dharmin Rokad, Shivani Ghaisas, Saurabh Verma, Alan Robertson, Huajun Jin, Vellareddy Anantharam, Arthi Kanthasamy, Anumantha G. Kanthasamy
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Review
Neurosciences
Dilshan S. Harischandra, Shivani Ghaisas, Gary Zenitsky, Huajun Jin, Arthi Kanthasamy, Vellareddy Anantharam, Anumantha G. Kanthasamy
FRONTIERS IN NEUROSCIENCE
(2019)
Article
Clinical Neurology
Sireesha Manne, Naveen Kondru, Huajun Jin, Vellareddy Anantharam, Xuemei Huang, Arthi Kanthasamy, Anumantha G. Kanthasamy
MOVEMENT DISORDERS
(2020)
Article
Neurosciences
Shivani Ghaisas, Monica R. Langley, Bharathi N. Palanisamy, Somak Dutta, Kirthi Narayanaswamy, Paul J. Plummer, Souvarish Sarkar, Muhammet Ay, Huajun Jin, Vellareddy Anantharam, Arthi Kanthasamy, Anumantha G. Kanthasamy
Article
Biotechnology & Applied Microbiology
Benjamin W. Schlichtmann, Monica Hepker, Bharathi N. Palanisamy, Manohar John, Vellareddy Anantharam, Anumantha G. Kanthasamy, Balaji Narasimhan, Surya K. Mallapragada
Summary: Synucleinopathies are debilitating neurodegenerative disorders with no clinically approved therapeutic options. Multiple synergistic pathological mechanisms and misfolding of proteins contribute to disease progression, making treatment challenging. Nanocarriers can improve brain delivery of therapeutics and enable multifunctional therapies.
CURRENT OPINION IN CHEMICAL ENGINEERING
(2021)
Article
Cell Biology
Sanjana Mahadev Bhat, Denusha Shrestha, Nyzil Massey, Locke A. Karriker, Anumantha G. Kanthasamy, Chandrashekhar Charavaryamath
Summary: Exposure to organic dust rich in microbial pathogen-associated molecular patterns can induce lung inflammation and mitochondrial stress, potentially contributing to airway diseases. The study showed significant changes in cellular and organelle morphology, as well as the induction of mitophagy, upon organic dust extract exposure. Mitochondrial dysfunction and stress may be partially ameliorated by treatments with ethyl pyruvate or mitoapocynin.
HISTOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Minhong Huang, Dan Lou, Adhithiya Charli, Dehui Kong, Huajun Jin, Gary Zenitsky, Vellareddy Anantharam, Arthi Kanthasamy, Zhibin Wang, Anumantha G. Kanthasamy
Summary: Research shows that mitochondrial dysfunction leads to increased H3K27ac acetylation in dopaminergic neuronal models of PD, opening vulnerable epigenomic loci, which is validated in PD-related neurodegenerative models and postmortem PD brains.