Article
Chemistry, Multidisciplinary
Beatrice Campanella, Laura Colombaioni, Riccardo Nieri, Edoardo Benedetti, Massimo Onor, Emilia Bramanti
Summary: Metabolomic profiling of the immortalized hippocampal neurons HN9.10e using a targeted approach revealed valuable insights into the extracellular metabolic environment of the cells. The study utilized a high-throughput reversed-phase liquid chromatography method and gas chromatography-mass spectrometry to analyze the metabolic reproducibility and starting conditions of the cell line. This research highlights the importance of understanding the metabolic profile of neural cells for future therapeutic interventions.
FRONTIERS IN CHEMISTRY
(2021)
Article
Neurosciences
Shaoqing Ma, Zhiwei Li, Shixiang Gong, Chengbiao Lu, Xiaoli Li, Yingwei Li
Summary: This study investigates the effect of 0.138 THz radiation on neuronal growth and synaptic transmission efficiency. The cumulative radiation promotes neuronal growth and improves synaptic transmission efficiency. This modulation can continue even after the radiation ends and is associated with an increase in dendritic spine density.
Article
Multidisciplinary Sciences
Angelo Forli, Michael M. Yartsev
Summary: This article examines hippocampal activity in groups of bats engaged in collective spatial behavior and finds that many hippocampal neurons are tuned to key features of group dynamics. Social responses are anatomically distributed and robustly represented at the population level.
Article
Neurosciences
Valentina Licheri, Giuseppe Talani, Giovanni Biggio, Enrico Sanna
Summary: It is well known that ethanol affects neuronal function through its interaction with membrane proteins and intracellular pathways. This study investigated the effect of ethanol on HCN channels in CA3 pyramidal neurons and found that ethanol modulates HCN-mediated Ih in a concentration-dependent and bi-directional manner. This modulation was mimicked by forskolin and antagonized by drugs that interfere with the AC/cAMP/PKA pathway and selective HCN inhibitor ZD7288.
Article
Multidisciplinary Sciences
Harris A. Weisz, Deborah R. Boone, William S. Coggins, Gabrielle A. Edwards, Hannah E. Willey, Steven G. Widen, Dionicio Siegel, Andrew T. Nelson, Donald S. Prough, Helen L. Hellmich
Summary: Evaluating the neuroprotective effects of novel compounds in animal models of traumatic brain injury (TBI) is time-consuming and expensive. This study demonstrates a rapid approach using an in vitro model to assess the pro-survival effects of three bioactive compounds, two isolated from natural products (clovanemagnolol [CM], vinaxanthone [VX]) and one dietary compound (pterostilbene [PT]). By examining gene and microRNA expression, the study provides mechanistic insights into the neuroprotective properties of these compounds. This approach could expedite the discovery of new TBI therapeutics by quickly assessing multiple natural products for their neuroprotective potential.
Article
Pharmacology & Pharmacy
Ying Zhang, Hai-Yan Yin, Patrizia Rubini, Peter Illes, Yong Tang
Summary: There is a dispute about whether CNS neurons possess ATP-sensitive P2X7 receptors. By genetically deleting P2X7 receptors in astrocytes and oligodendrocytes, it was found that these receptors indirectly modulate the neuronal input to neighboring neurons.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Mychael V. Lourenco, Guilherme B. de Freitas, Icaro Raony, Sergio T. Ferreira, Fernanda G. De Felice
Summary: Irisin promotes the accumulation of brain-derived neurotrophic factor (BDNF) and activates protective pathways in hippocampal neurons. Human hippocampal FNDC5 mRNA shows a trend of reduction with aging and Tau pathology.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Adi Doron, Alon Rubin, Aviya Benmelech-Chovav, Netai Benaim, Tom Carmi, Ron Refaeli, Nechama Novick, Tirzah Kreisel, Yaniv Ziv, Inbal Goshen
Summary: This study used two-photon microscopy to investigate the real-time calcium activity of astrocytes in the hippocampus of awake mice. The researchers found that astrocytes exhibit persistent ramping activity towards the reward location in a familiar environment, but not in a new one. Shifting the reward location within a familiar environment also resulted in diminished ramping. After additional training, the ramping activity was re-established.
Article
Neurosciences
Karen Safaryan, Mayank R. Mehta
Summary: Hippocampal theta rhythm plays a vital role in neuroplasticity, learning, and memory, with variations across species. Running in virtual reality greatly amplifies theta rhythmicity and can even lead to the emergence of a novel rhythm, indicating that multisensory experience influences hippocampal rhythms. Virtual reality can be utilized to enhance or regulate brain rhythms and influence neural dynamics, connections, and plasticity.
NATURE NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
J. Terreros-Roncal, E. P. Moreno-Jimenez, M. Flor-Garcia, C. B. Rodriguez-Moreno, M. F. Trinchero, F. Cafini, A. Rabano, M. Llorens-Martin
Summary: Disrupted hippocampal performance is a major factor contributing to psychiatric comorbidities and cognitive impairments in patients with neurodegenerative disorders. Adult hippocampal neurogenesis (AHN) plays a crucial role in several neurodegenerative diseases, with abnormalities found in adult-born dentate granule cells. Aging and diseases alter the neurogenic niche, reduce microglial phagocytic capacity, trigger astrogliosis, and change the microvasculature of the dentate gyrus, leading to enhanced vulnerability of AHN to neurodegeneration.
Article
Cell Biology
Barnabas Kocsis, Sergio Martinez-Bellver, Richard Fiath, Andor Domonkos, Katalin Sviatko, Daniel Schlingloff, Peter Bartho, Tamas F. Freund, Istvan Ulbert, Szabolcs Kali, Viktor Varga, Balazs Hangya
Summary: Episodic learning and memory retrieval are dependent on the GABAergic network of the medial septum, which achieves theta synchrony through synchronization of pacemakers and glutamatergic neurons. These findings provide experimental and theoretical support for a frequency-synchronization mechanism for pacing hippocampal theta.
Article
Toxicology
Azeezat A. Awoyemi, Christina Borchers, Langni Liu, Yanfang Chen, Christine M. Rapp, Chad A. Brewer, Ramzi Elased, Jeffrey B. Travers
Summary: Ethanol has been found to modulate the generation and release of larger microvesicle particles, providing a novel mechanism for ethanol-mediated effects, which could be relevant in the significant toxicity associated with thermal burn injury.
TOXICOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Valquiria Campos Alencar, Juliana de Fatima dos Santos Silva, Renata Ozelami Vilas Boas, Vinicius Manganaro Farnezio, Yara N. L. F. de Maria, David Aciole Barbosa, Alex Tramontin Almeida, Emanuel Maltempi de Souza, Marcelo Muller-Santos, Daniela L. Jabes, Fabiano B. Menegidio, Regina Costa de Oliveira, Tiago Rodrigues, Ivarne Luis dos Santos Tersariol, Adrian R. Walmsley, Luiz R. Nunes
Summary: AI-2 can induce QS responses in bacteria, and Z. mobilis responds to AI-2 by modulating gene expression related to QS, promoting ethanol production and adapting to the high energy demand of N-2 fixation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ruiyi Yuan, Younghee Hahn, Max H. Stempel, David K. Sidibe, Olivia Laxton, Jessica Chen, Aditi Kulkarni, Sandra Maday
Summary: Neurons and astrocytes have unique requirements for quality control pathways, such as autophagy and the UPS, to maintain proper function and survival. This study investigated the relationship between autophagy and the proteasome in primary astrocytes and neurons, and found that proteasome inhibition did not robustly upregulate autophagy. Instead, astrocytes formed Ub-positive aggregates in response to UPS inhibition, but these structures were not efficiently degraded by autophagy. Additionally, neurons were more vulnerable to proteotoxic stress compared to astrocytes.
Article
Engineering, Environmental
Angel Mario Lopez-Hidalgo, Gabriela Magana, Felicia Rodriguez, Antonio De Leon-Rodriguez, Arturo Sanchez
Summary: This study investigates the environmental and economic sustainability of a lignocellulose-based biorefinery by co-producing hydrogen and ethanol via dark fermentation using genetically engineered Escherichia coli. Results indicate that co-production schemes could be a viable alternative for lignocellulosic ethanol biorefineries.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Food Science & Technology
Luisa Pozzo, Cristina Alcantara, Marta Selma-Royo, Izaskun Garcia-Mantrana, Emilia Bramanti, Vincenzo Longo, Maria Carmen Collado, Laura Pucci
Summary: This study evaluated the impact of the fermentation process on the chemical composition and SCCs levels of spelt from Garfagnana, as well as its effect on in vitro microbial growth and metabolism. The fermentation process altered the composition of spelt and modulated the intestinal microbiota, potentially promoting gut health in both normal weight and obese individuals.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Environmental Sciences
Beatrice Campanella, Tommaso Lomonaco, Edoardo Benedetti, Massimo Onor, Riccardo Nieri, Federica Marmorino, Chiara Cremolini, Emilia Bramanti
Summary: This study proposes a method for direct determination of DHU in saliva for non-invasive screening and disease monitoring. Analysis of samples confirms that DHU is not the main metabolite in saliva and can be easily monitored using common, low-cost instrumentation.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Materials Science, Multidisciplinary
Emilia Bramanti, Lucio Bonaccorsi, Beatrice Campanella, Carlo Ferrari, Angela Malara, Angelo Freni
Summary: In this study, fibrous composites of TEOS/PVP were prepared using a sol-electrospinning process, and the correlation between their morphology, thermal stability, and spectroscopic features were analyzed. The results demonstrated that the addition of TEOS in the PVP/ethanol system enhanced the thermal stability of the microfibers.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Medicine, General & Internal
Edoardo Benedetti, Fabrizio Mavilia, Claudia Barate, Emilia Bramanti, Riccardo Morganti, Giulia Cervetti, Enrico Capochiani, Benedetto Bruno, Matteo Pelosini, Salvatore Massimo Stella, Sara Galimberti
Summary: This study suggests that ultrasound is a reliable, radiation-free tool for assessing superficial lymphnodes and splenic involvement in CLL patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Food Science & Technology
Teresa Grande, Aymen Souid, Marco Ciardi, Clara Maria Della Croce, Stefania Frassinetti, Emilia Bramanti, Vincenzo Longo, Luisa Pozzo
Summary: This study investigated the phenolic compounds, antioxidant activities, antimicrobial activity, and effect on Lactobacillus brevis growth of eight whole flours obtained from four ancient and modern wheat genotypes of Italian Triticum genus. The results showed quantitative differences in antioxidant activities, total phenolic content, and concentrations of five phenolic acids among the wheat genotypes. Wheat extracts had significant inhibitory effects on pathogenic bacteria and promoted the growth of L. brevis. The phenolic profile and antioxidant activities were influenced by the genotypic characteristics of the studied varieties, with the ancient Saragolla wheat standing out for its interesting phenolic profile.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Agricultural Engineering
Jose Gonzalez-Rivera, Beatrice Campanella, Elena Pulidori, Emilia Bramanti, Maria Rosaria Tine, Luca Bernazzani, Massimo Onor, Paolo Barberi, Celia Duce, Carlo Ferrari
Summary: Novel green extraction method (US-IMWAE) using simultaneous ultrasound and microwave irradiation allows for full exploitation of aromatic herbs while minimizing energy consumption and extraction cost. This method has successfully yielded essential oils with appealing composition profiles for cosmetic and medical industries, as well as valuable solid residues for alternative fuel production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Physical
Elena Pulidori, Simone Micalizzi, Nikos Koutsomarkos, Emilia Bramanti, Maria Rosaria Tine, Giovanni Vozzi, Carmelo De Maria, Maria Chatzinikolaidou, Celia Duce
Summary: This study investigates the structure and correlations between structure, mechanical, and biological properties of gelatin-based materials. Gelatin electro-spun materials have higher content of ordered structure compared to gelatin casted films, which may be attributed to the random coil - alpha-helix transition during electrospinning. The presence of cross linker (GPTMS) decreases the ordered structures in gelatin casted films, while it does not affect the percentage of ordered structure in electrospun samples. The presence of keratin in gelatin/keratin electrospun biomaterials decreases alpha-helix content and promotes the conversion from antiparallel to parallel beta-sheet.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Multidisciplinary Sciences
Ophelie Ranquet, Celia Duce, Emilia Bramanti, Patrick Dietemann, Ilaria Bonaduce, Norbert Willenbacher
Summary: The authors use a combination of egg yolk and two pigments to investigate how different distribution of proteinaceous binder can control the flow behavior, drying kinetics, and chemistry of oil paints. This study demonstrates that adjusting the distribution of proteinaceous binder can enhance the stiffening effect and prevent undesired absorption of humidity, improving the brushability and preserving invaluable artworks.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Edoardo Benedetti, Claudia Barate, Fabrizio Mavilia, Emilia Bramanti, Riccardo Morganti, Valentina Guerri, Giulia Cervetti, Enrico Capochiani, Ilaria Bertaggia, Salvatore Massimo Stella, Ginevra Traverso, Benedetto Bruno, Sara Galimberti
Summary: Fixed duration VenR treatment showed significant improvements in PFS and uMRD compared to bendamustine-rituximab in relapsed/refractory CLL patients. Ultrasonography and palpation were suggested as possible techniques to evaluate visceral and superficial lymph node involvement outside of clinical trials.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Beatrice Campanella, Stefano Legnaioli, Massimo Onor, Edoardo Benedetti, Emilia Bramanti
Summary: Saliva is a sample that can be easily collected and contains a wealth of biochemical information related to an individual's health status. The fast and straightforward analysis of saliva using vibrational spectroscopy provides a good basis for large-scale preclinical studies. This study explored the effects of saliva collection (spitting/swab) and processing (deproteinization procedures) and found that special care is needed when using swabs due to the significant interaction between the polymeric material and saliva components. Additionally, analyzing saliva before and after deproteinization by FTIR and Raman spectroscopy provides complementary biological information.
Article
Chemistry, Analytical
Michelle Buoso, Beatrice Campanella, Massimo Onor, Emanuela Pitzalis, Alessandro D'Ulivo
Summary: The use of BH intermediates as derivatization reagents significantly improves the tolerance to interference from transition metal ions in the generation of SbH3 and BiH3, and shows high tolerance in a wide range of acidity. The method has been successfully applied to the determination of Sb and Bi in complex samples, providing further understanding of the chemical behavior of borohydrides.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)