Article
Plant Sciences
Xiuhong Gou, Chengcheng Zhong, Peiling Zhang, Liru Mi, Yilin Li, Wenqin Lu, Jie Zheng, Junjie Xu, Yuling Meng, Weixing Shan
Summary: Oomycetes, as diploid eukaryotic microorganisms, pose a serious threat to crop production. This study investigates the roles of miR398b and its cis-NAT, AtC2GnT, in plant immunity against oomycete pathogens. The results show that miR398b targets CSD1 and CSD2 genes to suppress their expression, leading to decreased plant disease resistance. AtC2GnT inhibits the miR398b-CSDs module by downregulating pri-miR398b expression, resulting in elevated plant resistance to the pathogen. These findings reveal the positive role of AtC2GnT and the negative regulatory loop formed by miR398b and AtC2GnT in regulating plant resistance to oomycetes.
Article
Multidisciplinary Sciences
Weichao Wang, Jiayin Pang, Fenghua Zhang, Lupeng Sun, Lei Yang, Tingdong Fu, Liang Guo, Kadambot H. M. Siddique
Summary: Salinity is a major constraint on crop growth and productivity, but understanding the molecular mechanisms of salt-stress adaptation in canola can improve salt tolerance and promote its cultivation in saline lands. In this study, DEGs involved in signal transduction and ROS scavenging were highly expressed under salt stress, and different stages of salt stress affected canola transcript expression differently. Additionally, several transcription factor families associated with salt tolerance were identified. These findings provide a basis for further research on salt stress adaptation in canola.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Gang Lei, Kun-Hua Zhou, Xue-Jun Chen, Yue-Qin Huang, Xin-Jie Yuan, Ge-Ge Li, Yuan-Yuan Xie, Rong Fang
Summary: This study investigated the molecular response of pepper to Phytophthora capsici infection through transcriptome and metabolome analysis. The findings showed that more genes and metabolites were induced in the resistant genotype, indicating a prompt activation of defense responses. The resistant genotype exhibited fine regulation mediated by calcium and salicylic acid signaling pathways, leading to the activation of downstream defense responses. Genes and metabolites involved in flavonoid biosynthesis pathways were uniquely activated in the resistant genotype, highlighting the importance of these pathways in pepper resistance to P. capsici.
Article
Plant Sciences
Jinfang Li, Fengyan Deng, Hongmei Wang, Xiaoyu Qiang, Yuling Meng, Weixing Shan
Summary: The study identified a novel plant susceptibility factor, a Raf-like kinase Raf36, which regulates Arabidopsis susceptibility to Phytophthora parasitica upstream of MKK2 and directly targets it to negatively regulate plant resistance to P. parasitica.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Cell Biology
Mingyue Li, Miao Bai, Yulun Wu, Shuo Yang, Lihua Zheng, Luguo Sun, Chunlei Yu, Yanxin Huang
Summary: Gastric adenocarcinoma (GAC) is a highly lethal malignant tumor with unclear pathogenesis. This study analyzed the gene expression profiles of GAC tumor tissues and adjacent normal tissues, identifying 465 differentially expressed genes (DEGs). Further analysis revealed enrichment of DEGs in pathways related to extracellular matrix, protein digestion and absorption, and gastric acid secretion. Six key genes associated with poor GAC prognosis were identified, including P4HA3 and MATN3, which have rarely been reported in relation to GAC. Functional analysis demonstrated that upregulation of P4HA3 enhanced the proliferation and migration ability of GAC cells. This study provides new insights for the selection of prognostic markers and therapeutic targets for GAC.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Plant Sciences
Yun-Yao Yang, Bo Ma, Ying-Ying Li, Ming-Zheng Han, Jing Wu, Xiao-Feng Zhou, Ji Tian, Wen-He Wang, Ping-Sheng Leng, Zeng-Hui Hu
Summary: In this study, the molecular mechanism of monoterpene synthesis in Lilium was investigated. The content of three main monoterpenes was examined and RNA sequencing of flower development stages was conducted. Differentially expressed genes involved in plant hormone signal transduction and monoterpenoid biosynthesis were identified. Silencing of the key factor LiMYB305 resulted in a decrease in monoterpene synthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Respiratory System
Delphine Guillotin, Adam R. Taylor, Manuela Plate, Paul F. Mercer, Lindsay M. Edwards, Ross Haggart, Gino Miele, Robin J. McAnulty, Toby M. Maher, Robert E. Hynds, Mariam Jamal-Hanjani, Richard P. Marshall, Andrew J. Fisher, Andy D. Blanchard, Rachel C. Chambers
Summary: This study aimed to identify major transcriptional programs in idiopathic pulmonary fibrosis (IPF) by profiling myofibroblasts in fibrotic foci using novel data mining strategies and weighted gene co-expression network analysis (WGCNA). The findings revealed clusters of genes associated with cell cycle, inflammation/differentiation, translation, and cytoskeleton/cell adhesion, with key signaling pathways like TGF-beta 1, RhoA kinase, and the TSC2/RHEB axis influencing collagen gene expression. Functional studies using CRISPR-Cas9 gene-edited cells demonstrated the potential role of the TSC2/RHEB axis in regulating excessive matrix deposition in IPF and other fibrotic conditions.
Article
Biochemistry & Molecular Biology
Anupam Gogoi, Erik Lysoe, Havard Eikemo, Arne Stensvand, Jahn Davik, May Bente Brurberg
Summary: In this study, comparative transcriptome analysis revealed several candidate disease resistance genes in the diploid strawberry species F. vesca that provide resistance to P. cactorum. These genes mainly encode disease resistance proteins. Specifically, 35-38 genes in the RPc-1 locus of F. vesca showed differential expression in response to P. cactorum. These findings provide new insights into the plant defense mechanisms against P. cactorum and offer potential targets for further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Zhanchao Cheng, Changhong Mu, Xiangyu Li, Wenlong Cheng, Miaomiao Cai, Chongyang Wu, Jutang Jiang, Hui Fang, Yucong Bai, Huifang Zheng, Ruiman Geng, Junlei Xu, Yali Xie, Yuping Dou, Juan Li, Shaohua Mu, Jian Gao
Summary: In this study, a modified protocol for protoplast preparation was used to investigate the single-cell transcriptome of the root system in moso bamboo. Four cell types were identified, and new cell-type-specific marker genes for the basal root were defined. The developmental trajectories of the root cap, epidermis, and ground tissues were reconstructed, and key factors regulating cell fate determination were elucidated. Furthermore, the study revealed the divergence of root developmental pathways between moso bamboo and rice and identified key regulatory factors for cell differentiation.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Hanwen Yu, Mengli Liu, Minzhen Yin, Tingyu Shan, Huasheng Peng, Jutao Wang, Xiangwei Chang, Daiyin Peng, Liangping Zha, Shuangying Gui
Summary: Through comprehensive transcriptome analysis using different sequencing technologies, genes related to triterpenoid saponin biosynthesis were identified in various tissues of Platycodon grandiflorus. The study also validated the association of certain genes with triterpenoid saponin biosynthesis.
Article
Environmental Sciences
Youngjae Pyo, Heewon Moon, Adji Baskoro Dwi Nugroho, Seong Wook Yang, Il Lae Jung, Dong-Hwan Kim
Summary: Strontium, particularly its radioactive isotope Sr-90, poses a global threat to the environment and human health. The molecular mechanisms underlying plant response to Sr toxicity remain largely unknown. This study used RNA-seq analysis to compare gene expression profiles between control and Sr-treated plants, revealing that the JA biosynthesis and/or signaling pathway may play important roles in plant response to Sr stress, maintaining proper growth and development.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Jinsen Cai, Songguang Yang, Wenrui Liu, Jinqiang Yan, Biao Jiang, Dasen Xie
Summary: In this study, the gene expression profiles of two Benincasa hispida cultivars with different resistance to Phytophthora melonis were analyzed. The results showed that phenylpropanoid biosynthesis, plant-pathogen interaction, the MAPK signaling pathway-plant, and plant hormone signal transduction played important roles in the response of Benincasa hispida to Phytophthora melonis infection.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Li Li, Yanfang Wang, Mingzhu Zhao, Kangyu Wang, Chunyu Sun, Lei Zhu, Yilai Han, Ping Chen, Jun Lei, Yi Wang, Meiping Zhang
Summary: At least five PgOSC genes are involved in ginsenoside biosynthesis, including three newly identified genes and two published genes. These genes show significant correlations with ginsenoside contents and their mutations influence ginsenoside levels. The regulation analysis using MeJA further supports the role of these genes in ginsenoside biosynthesis.
Article
Immunology
Yi Xiang, Quzhe Emu, Li Wang, Yong Wei, Liu Xing, Ling Zhang, Haipeng Wang
Summary: This study revealed the immune response mechanism of the spleen in A. nidulan-infected mice and identified differentially expressed genes related to immune response. The findings are important for a better understanding of the pathogenic mechanisms of A. nidulans.
MICROBIAL PATHOGENESIS
(2022)
Article
Plant Sciences
Fen Zhang, Wei He, Qingyun Yuan, Kang Wei, Li Ruan, Liyuan Wang, Hao Cheng
Summary: This research analyzed the effect of nitrogen starvation and resupply on nitrogen uptake in tea plants, identifying key genes in the CsNRT gene family for nitrogen uptake. The overexpression of CsNRT2.4 in transgenic Arabidopsis thaliana improved nitrate uptake efficiency, suggesting its role as a key gene for nitrogen uptake in tea plant roots. The study provides valuable insights into enhancing nitrogen usage efficiency in tea plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Evolutionary Biology
Anne Dievart, Nicolas Gilbert, Gaetan Droc, Agnes Attard, Matthieu Gourgues, Emmanuel Guiderdoni, Christophe Perin
BMC EVOLUTIONARY BIOLOGY
(2011)
Article
Biotechnology & Applied Microbiology
Agnes Attard, Edouard Evangelisti, Naima Kebdani-Minet, Franck Panabieres, Emeline Deleury, Cindy Maggio, Michel Ponchet, Mathieu Gourgues
Article
Plant Sciences
Agnes Attard, Mathieu Gourgues, Eric Galiana, Franck Panabieres, Michel Ponchet, Harald Keller
JOURNAL OF PLANT PHYSIOLOGY
(2008)
Review
Biochemistry & Molecular Biology
Sophie Hok, Agnes Attard, Harald Keller
MOLECULAR PLANT-MICROBE INTERACTIONS
(2010)
Article
Plant Sciences
Agnes Attard, Mathieu Gourgues, Nicolas Callemeyn-Torre, Harald Keller
Article
Plant Sciences
Edouard Evangelisti, Benjamin Govetto, Naima Minet-Kebdani, Marie-Line Kuhn, Agnes Attard, Michel Ponchet, Franck Panabieres, Mathieu Gourgues
Article
Plant Sciences
Sophie Hok, Etienne G. J. Danchin, Valerie Allasia, Franck Panabieres, Agnes Attard, Harald Keller
PLANT CELL AND ENVIRONMENT
(2011)
Article
Plant Sciences
Marco Uwe Reitz, Jeff Kweku Bissue, Kathleen Zocher, Agnes Attard, Ralph Hueckelhoven, Katja Becker, Jafargholi Imani, Ruth Eichmann, Patrick Schaefer
Article
Plant Sciences
Sophie Hok, Valerie Allasia, Emilie Andrio, Elodie Naessens, Elsa Ribes, Franck Panabieres, Agnes Attard, Nicolas Ris, Mathilde Clement, Xavier Barlet, Yves Marco, Erwin Grill, Ruth Eichmann, Corina Weis, Ralph Hueckelhoven, Alexandra Ammon, Jutta Ludwig-Mueller, Lars M. Voll, Harald Keller
Article
Plant Sciences
Romain Castilleux, Barbara Plancot, Bruno Gugi, Agnes Attard, Corinne Loutelier-Bourhis, Benjamin Lefranc, Eric Nguema-Ona, Mustapha Arkoun, Jean-Claude Yvin, Azeddine Driouich, Maite Vicre
Article
Microbiology
Christian Froschel, Jaqueline Komorek, Agnes Attard, Alexander Marsell, William A. Lopez-Arboleda, Joelle Le Berre, Elmar Wolf, Niko Geldner, Frank Waller, Arthur Korte, Wolfgang Droge-Laser
Summary: The research revealed that plant roots have genetically defined response programs in different cell layers when facing various microbial infections, highlighting the importance of radial organization in root-microbe interactions. The study utilized cell-layer translatomes to gain insights into the different colonization strategies of pathogens and mutualists, providing a valuable resource for crop improvement strategies.
CELL HOST & MICROBE
(2021)
Article
Biochemistry & Molecular Biology
Ilaria Bassani, Marie Larousse, Quang D. Tran, Agnes Attard, Eric Galiana
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2020)
Article
Plant Sciences
MH Balesdent, A Attard, AL Kühn, T Rouxel
Article
Genetics & Heredity
A Attard, L Gout, S Ross, F Parlange, L Cattolico, MH Balesdent, T Rouxel
FUNGAL GENETICS AND BIOLOGY
(2005)