Article
Plant Sciences
Haodong Huang, Xianpeng Yang, Minglu Zheng, Shiyou Lu, Huayan Zhao
Summary: This study reveals the regulatory roles of KCS3 and KCS12 in cuticle biosynthesis. Mutations in KCS3 have a greater impact on wax production, while mutations in KCS12 exert more severe effects on cutin synthesis. KCS12 and KCS3 have additive effects on cuticle formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Entomology
Gary J. Blomquist, Matthew D. Ginzel
Summary: Insect cuticular hydrocarbons (CHCs) have evolved to serve both waterproofing and signaling functions, playing critical roles in chemical communication and life histories. The profiles of CHCs are influenced by diet and environment, and recent advances in understanding hydrocarbon biosynthesis have utilized molecular biology tools such as genomics and RNA interference.
ANNUAL REVIEW OF ENTOMOLOGY, VOL 66, 2021
(2021)
Article
Food Science & Technology
Wenqi Shi, Jie Li, Yanfang Chen, Yefu Chen, Xuewu Guo, Dongguang Xiao
Summary: Metabolic engineering strategies were utilized to enhance the production of C6-C10 fatty acid ethyl esters in Saccharomyces cerevisiae, including improving precursor accumulation, increasing alcohol acyltransferase activity, and facilitating ester synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Karolis Petkevicius, Eleni Koutsoumpeli, Petri Christina Betsi, Bao-Jian Ding, Kanchana Rueksomtawin Kildegaard, Hilbert Jensen, Nora Mezo, Andrea Mazziotta, Anders Gabrielsson, Christina Sinkwitz, Bettina Lorantfy, Carina Holkenbrink, Christer Lofstedt, Dimitris Raptopoulos, Maria Konstantopoulou, Irina Borodina
Summary: This study developed a biotechnological method to produce the sex pheromone of the European corn borer using engineered yeast, achieving high titers of the pheromone component. The research opens up possibilities for fermentation-based production of ECB pheromones for pest management.
BIOTECHNOLOGY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Sylwia Szrok-Jurga, Aleksandra Czumaj, Jacek Turyn, Areta Hebanowska, Julian Swierczynski, Tomasz Sledzinski, Ewa Stelmanska
Summary: Fatty acid metabolism, especially beta-oxidation, plays a crucial role in human physiology and pathology. Beta-oxidation is not only involved in energy metabolism but also contributes to the synthesis of various important compounds. This review provides an overview of the importance of mitochondrial and peroxisomal beta-oxidation in different organs and cells and discusses the diseases associated with fatty acid oxidation disturbances. The review also highlights the abnormalities of fatty acid oxidation in cancer cells and diseases caused by mutations in enzymes involved in fatty acid oxidation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Laura A. St Clair, Stephanie A. Mills, Elena Lian, Paul S. Soma, Aritra Nag, Caroline Montgomery, Gabriela Ramirez, Nunya Chotiwan, Rebekah C. Gullberg, Rushika Perera
Summary: During infection with dengue viruses, the lipid landscape within host cells is altered to support viral replication and particle assembly. Fatty acyl-CoAs, which are key intermediates in lipid biosynthesis, play a role in this process. Loss of function of certain enzymes involved in fatty acyl-CoA metabolism leads to increased viral replication and infectious particle release, while loss of others results in decreased protein translation, genome replication, and virus release. The specific location of these enzymes within the cell may contribute to the differences observed in dengue virus infection. Loss of mitochondrial ACOT2 and ACOT7 expression also affects the expression of other ACOTs located in different organelles within the cell, suggesting a complex relationship between ACOTs in the virus lifecycle.
Article
Biochemistry & Molecular Biology
Kento Otsuka, Megumi Sawai-Ogawa, Akio Kihara
Summary: Fatty acyl-CoA reductases (FARs) play a crucial role in the synthesis of meibum lipids, which are important for tear stability and eye health. This study reveals the specific functions of two FAR isozymes (FAR1 and FAR2) in the production of different chain lengths of fatty alcohols (FAls), and highlights the importance of FAl-containing meibum lipids in the formation of a functional tear film lipid layer.
Article
Biochemistry & Molecular Biology
Ravi R. Sonani, Artur Blat, Grzegorz Dubin
Summary: This study solved the crystal structures of human ACOX1a, revealing its homodimeric form and the binding of two FAD molecules at the active site. It also found that the substrate binding cleft of ACOX1a is wider compared to other fatty acid oxidation enzymes. Furthermore, the study analyzed the impact of mutations causing dysfunctionality of ACOX1a on its structure and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Medicinal
Kyohei Hayashi, Noriyasu Kondo, Naoki Omori, Ryo Yoshimoto, Megumi Hato, Shuhei Shigaki, Ayumi Nagasawa, Mana Ito, Takayuki Okuno
Summary: Long-chain acyl-CoA synthetase-1 (ACSL1) is believed to play essential roles in lipid metabolism. Compound 13, as the first selective and highly potent ACSL1 inhibitor, showed remarkable inhibitory activity against ACSL1 and excellent selectivity for other ACSL isoforms, as well as demonstrated an in vivo suppression effect against the production of long-chain acyl-CoAs in mice.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Microbiology
Mason H. H. Lee, Gangqing Hu, Rita V. M. Rio
Summary: Tsetse flies are the only vectors of African trypanosomes, and they harbor essential Wigglesworthia glossinidia bacteria. The differential expression of these bacteria and the interaction between miRNAs and mRNAs in tsetse flies offer potential for population control strategies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuxun Zhang, Eric Goetzman
Summary: Mitochondrial trifunctional protein (TFP) plays a crucial role in chain-shortening long-chain fatty acids within the mitochondria. Despite being heavily modified by acetyllysine and succinyllysine post-translational modifications, the functional significance of these modifications on TFP remains unclear. Research suggests that TFP in the membrane-bound state may be protected from lysine acylation, which could impact its enzymatic activity.
Article
Pharmacology & Pharmacy
Hiroki Hashizume, Tatsuki Fukami, Kanji Mishima, Hiroshi Arakawa, Kenji Mishiro, Yongjie Zhang, Masataka Nakano, Miki Nakajima
Summary: This study aimed to clarify the human ACS isoforms responsible for CoA-conjugation of NSAIDs by considering the hepatic expression levels of ACS isoforms. Among the 10 types of NSAIDs studied, propionic acid-class NSAIDs were found to be conjugated with CoA in the human liver, while NSAIDs in other classes did not exhibit this reaction. ACSL1 was identified as the most highly expressed ACS isoform in the human liver, and could catalyze the CoA conjugation of propionic acid-class NSAIDs, which may lead to toxicity through protein adduct formation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Veronika Mater, Sabine Eisner, Cornelia Seidel, Dirk Schneider
Summary: Tuberculosis, a common infectious disease, infects about one-fourth of the global population. The causative agent, Mycobacterium tuberculosis, can enter a dormant state and display antibiotic resistance. The study investigates the membrane association and substrate specificity of MbFACL6, a potential target for treating resistant strains. MbFACL6 is found to be a peripheral membrane-attached protein and shows a broad spectrum of substrate tolerance.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Franck Paris, Yuko Krzyzaniak, Christian Gauvrit, Frank Jamois, Frederic Domergue, Jerome Joubes, Vincent Ferrieres, Marielle Adrian, Laurent Legentil, Xavier Daire, Sophie Trouvelot
PHYSIOLOGIA PLANTARUM
(2016)
Article
Plant Sciences
Daniela Hegebarth, Christopher Buschhaus, May Wu, David Bird, Reinhard Jetter
Article
Biochemistry & Molecular Biology
L. Boureau, A. How-Kit, E. Teyssier, S. Drevensek, M. Rainieri, J. Joubes, L. Stammitti, A. Pribat, C. Bowler, Y. Hong, P. Gallusci
PLANT MOLECULAR BIOLOGY
(2016)
Article
Plant Sciences
Camille Delude, Laetitia Fouillen, Palash Bhar, Marie-Josee Cardinal, Stephanie Pascal, Patricia Santos, Dylan K. Kosma, Jerome Joubes, Owen Rowland, Frederic Domergue
Article
Plant Sciences
Johann Petit, Cecile Bres, Jean-Philippe Mauxion, Fabienne Wong Jun Tai, Laetitia B. B. Martin, Eric A. Fich, Jerome Joubes, Jocelyn K. C. Rose, Frederic Domergue, Christophe Rothan
Article
Plant Sciences
Sarah J. Shumborski, A. Lacey Samuels, David A. Bird
Article
Plant Sciences
Rebecca A. Smith, Mathias Schuetz, Steven D. Karlen, David Bird, Naohito Tokunaga, Yasushi Sato, Shawn D. Mansfield, John Ralph, A. Lacey Samuels
Article
Plant Sciences
Stephanie Pascal, Amelie Bernard, Paul Deslous, Julien Gronnier, Ashley Fournier-Goss, Frederic Domergue, Owen Rowland, Jerome Joubes
Article
Genetics & Heredity
Audrey Creff, Lysiane Brocard, Jerome Joubes, Ludivine Taconnat, Nicolas M. Doll, Anne-Charlotte Marsollier, Stephanie Pascal, Roberta Galletti, Sophy Boeuf, Steven Moussu, Thomas Widiez, Frederic Domergue, Gwyneth Ingram
Review
Cell Biology
Marguerite Batsale, Delphine Bahammou, Laetitia Fouillen, Sebastien Mongrand, Jerome Joubes, Frederic Domergue
Summary: Very-long-chain fatty acids (VLCFA) are crucial molecules in plants, playing roles in membrane lipids, cuticle waxes, root barrier components, and seed germination. Their importance lies in membrane homeostasis, plant adaptation, and seed development.
Article
Multidisciplinary Sciences
Rodrigo Enrique Gomez, Clement Chambaud, Josselin Lupette, Julie Castets, Stephanie Pascal, Lysiane Brocard, Lise Noack, Yvon Jaillais, Jerome Joubes, Amelie Bernard
Summary: Autophagosomes are specialized vesicles that target and deliver cargo to the lytic vacuole. This study reveals the importance of plasma-membrane derived lipid phosphatidylinositol-4-phosphate in the assembly and expansion of autophagosomes in Arabidopsis.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Cell Biology
Rodrigo Enrique Gomez, Julie Castets, Josselin Lupette, Clement Chambaud, Jerome Joubes, Amelie Bernard
Summary: Autophagy is a key mechanism in plants that helps them adapt to various environmental constraints. The molecular mechanisms, especially autophagosome formation, remain poorly understood. A recent study used biochemistry, genetics, cell biology, and high-resolution 3D imaging to uncover the function of lipid phosphatidylinositol-4-phosphate (PtdIns4P) in autophagy in Arabidopsis thaliana, providing new insights into autophagosome assembly in plant cells.
Article
Biochemistry & Molecular Biology
Urszula Luzarowska, Anne-Kathrin Russ, Jerome Joubes, Marguerite Batsale, Jedrzej Szymanski, Venkatesh P. Thirumalaikumar, Marcin Luzarowski, Si Wu, Feng Zhu, Niklas Endres, Sarah Khedhayir, Julia Schumacher, Weronika Jasinska, Ke Xu, Sandra Marcela Correa Cordoba, Simy Weil, Aleksandra Skirycz, Alisdair Robert Fernie, Yonghua Li-Beisson, Corina M. Fusari, Yariv Brotman
Summary: Plant lipids are important as alternative sources of carbon and energy when sugars or starch are limited. The study found that natural allelic variation at the KCS4 gene determines the differential accumulation of polyunsaturated triacylglycerols under stress. KCS4 is a functional enzyme involved in VLCFA synthesis and its allelic mutants and overexpression revealed its differential role in various processes related to lipid metabolism.