Article
Plant Sciences
Shuang Liu, Meixuezi Tong, Lifang Zhao, Xin Li, Ljerka Kunst
Summary: The outer epidermal cell walls of plant shoots are covered with a continuous lipid structure called a cuticle, providing protection from various environmental factors. Cuticular wax synthesis is tightly regulated by transcriptional and post-transcriptional mechanisms, as well as via the ubiquitin-26S proteasome system. An E3 ubiquitin ligase, AtARRE, has been identified as a negative regulator of wax biosynthesis in Arabidopsis, affecting the alkane-forming branch of the pathway.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Saet Buyl Lee, Mi Chung Suh
Summary: This study summarizes the regulatory mechanisms underlying cuticular wax biosynthesis in plants, including transcriptional, post-transcriptional, post-translational, and epigenetic levels. The advances in our understanding of these regulatory mechanisms have improved our knowledge of how plants balance carbon metabolic pathways between optimal growth and development and defense against abiotic stresses.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Melissa S. Traver, Bonnie Bartel
Summary: Lipid droplets are conserved organelles that regulate energy homeostasis by storing and releasing neutral lipids. This study identified MIEL1 as a protein that modulates lipid droplet dynamics by ubiquitinating and degrading the seed lipid droplet protein OLE1. The human homolog of MIEL1, PIRH2, was also found to be involved in lipid catabolism and peroxisome biology in mammals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Huimin Sun, Jiatao Li, Xu Li, Qian Lv, Liuping Chen, Bingxin Wang, Liqun Li
Summary: TaSADR1 gene was found to negatively regulate drought stress response in wheat and achieved this function by regulating the expression of stress-related genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Article
Biochemistry & Molecular Biology
Jiayi Yu, Lu Kang, Yuanyuan Li, Changai Wu, Chengchao Zheng, Pei Liu, Jinguang Huang
Summary: This study identified that RGLG1 and RGLG2 negatively regulate the drought stress tolerance mediated by MAPKKK18 in Arabidopsis by promoting the degradation of MAPKKK18 through ubiquitination. Deletion of RGLG1 and RGLG2 stabilizes MAPKKK18 and enhances drought stress tolerance in MAPKKK18-overexpression plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
Camilla Stanton, Jorge Rodriguez-Celma, Ute Kramer, Dale Sanders, Janneke Balk
Summary: Mutation of two E3 ligases that suppress iron uptake in roots also confers tolerance to zinc toxicity, identifying a regulatory point of interaction between iron and zinc homeostasis. Loss of function of BTSL1 and BTSL2, which encode E3 ubiquitin ligases, leads to Arabidopsis thaliana having tolerance to excess zinc. These mutants have similar zinc accumulation as the wild type, but lower iron accumulation in roots.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Maria Cecilia Terrile, Nuria Malena Tebez, Silvana Lorena Colman, Julieta Lisa Mateos, Esperanza Morato-Lopez, Nuria Sanchez-Lopez, Alicia Izquierdo-Alvarez, Anabel Marina, Luz Irina A. Calderon Villalobos, Mark Estelle, Antonio Martinez-Ruiz, Diego Fernando Fiol, Claudia Anahi Casalongue, Maria Jose Iglesias
Summary: E3 ubiquitin ligases are crucial in plant biology and play a key role in the perception and signaling of hormones. The redox modification of proteins through S-nitrosation serves as an important regulatory mechanism in hormonal signaling pathways. This study confirmed the S-nitrosation of TIR1 and COI1, highlighting the functional relevance of this modification in the response to auxins and jasmonates.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Seung-Heon Baek, Yeun-Kyu Jang
Summary: AMBRA1 negatively regulates ALDH1B1 through noncanonical ubiquitination, mediated by cooperation with E3 ligases like TRAF6. It controls the K27- and K33-linked ubiquitination of ALDH1B1, impacting its expression and self-association ability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Aiqin Sun, Jun Zhu, Song Xia, Yanling Li, Tiantian Wu, Genbao Shao, Wannian Yang, Qiong Lin
Summary: The study revealed that NEDD4 interacts with MEKK5 through a conserved WW3 domain, but MEKK5 is not a ubiquitination substrate of NEDD4 and instead negatively regulates NEDD4-mediated ubiquitination. Overexpression of MEKK5 significantly reduces NEDD4-promoted lung cancer cell migration.
Article
Plant Sciences
Katrina J. Linden, Mon Mandy Hsia, Yi-Tze Chen, Judy Callis
Summary: The E3 ligase BRIZ in Arabidopsis thaliana plays a crucial role in plant hormone signaling pathways, particularly in suppressing ABA signaling. Mutants of briz exhibit hypersensitivity to ABA, and BRIZ acts by inhibiting ABA signaling to affect seed development and germination.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
