Genetic and metabolic engineering in diatoms

Identification of a triacylglycerol lipase in the diatom Phaeodactylum tricornutum

(2016) Frederik Barka et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

The physiology and genetics of CO 2 concentrating mechanisms in model diatoms

(2016) Brian M Hopkinson et al.   CURRENT OPINION IN PLANT BIOLOGY

Diatom Vacuolar 1,6-β-Transglycosylases can Functionally Complement the Respective Yeast Mutants

(2016) Weichao Huang et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Multisignal control of expression of the LHCX protein family in the marine diatomPhaeodactylum tricornutum

(2016) Lucilla Taddei et al.   JOURNAL OF EXPERIMENTAL BOTANY

Identification of genes coding for functional zeaxanthin epoxidases in the diatom Phaeodactylum tricornutum

(2016) Ulrike Eilers et al.   JOURNAL OF PLANT PHYSIOLOGY

Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum

(2016) Mary Hamilton et al.   Marine Drugs

Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation

(2016) Bao-Hua Zhu et al.   New Biotechnology

Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean

(2016) Antonio Emidio Fortunato et al.   PLANT CELL

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

Thylakoid luminal θ-carbonic anhydrase critical for growth and photosynthesis in the marine diatomPhaeodactylum tricornutum

(2016) Sae Kikutani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Addressing various compartments of the diatom model organism Phaeodactylum tricornutum via sub-cellular marker proteins

(2016) Xiaojuan Liu et al.   Algal Research-Biomass Biofuels and Bioproducts

Rapid induction of GFP expression by the nitrate reductase promoter in the diatomPhaeodactylum tricornutum

(2016) Lili Chu et al.   PeerJ

Recent advances in microbial production of fuels and chemicals using tools and strategies of systems metabolic engineering

(2015) Changhee Cho et al.   BIOTECHNOLOGY ADVANCES

Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage

(2015) Marie JJ Huysman et al.   BMC PLANT BIOLOGY

Limitations in the biosynthesis of fucoxanthin as targets for genetic engineering in Phaeodactylum tricornutum

(2015) Ulrike Eilers et al.   JOURNAL OF APPLIED PHYCOLOGY

Biodiversity of NPQ

(2015) Reimund Goss et al.   JOURNAL OF PLANT PHYSIOLOGY

Establishment of Genetic Transformation in the Sexually Reproducing Diatoms Pseudo-nitzschia multistriata and Pseudo-nitzschia arenysensis and Inheritance of the Transgene

(2015) Valeria Sabatino et al.   MARINE BIOTECHNOLOGY

Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum

(2015) Takashi Kadono et al.   Marine Drugs

Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum

(2015) Lina-Juana Dolch et al.   Marine Drugs

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

(2015) Jiao Xue et al.   METABOLIC ENGINEERING

Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms

(2015) Benjamin Bailleul et al.   NATURE

An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatomPhaeodactylum tricornutum

(2015) Orly Levitan et al.   PLANT JOURNAL

Biochemical Characterization of Human Anti-Hepatitis B Monoclonal Antibody Produced in the Microalgae Phaeodactylum tricornutum

(2015) Gaëtan Vanier et al.   PLoS One

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Targeted drug delivery using genetically engineered diatom biosilica

(2015) Bahman Delalat et al.   Nature Communications

Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum

(2015) Takashi Kadono et al.   Scientific Reports

Development of a new constitutive expression system for the transformation of the diatom Phaeodactylum tricornutum

(2015) Seungbeom Seo et al.   Algal Research-Biomass Biofuels and Bioproducts

Towards the Industrial Production of Omega-3 Long Chain Polyunsaturated Fatty Acids from a Genetically Modified Diatom Phaeodactylum tricornutum

(2015) Mary L. Hamilton et al.   PLoS One

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Delta 5 Fatty Acid Desaturase Upregulates the Synthesis of Polyunsaturated Fatty Acids in the Marine Diatom Phaeodactylum tricornutum

(2014) Kun-Tao Peng et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Reduction in Carotenoid Levels in the Marine Diatom Phaeodactylum tricornutum by Artificial MicroRNAs Targeted Against the Endogenous Phytoene Synthase Gene

(2014) Simrat Kaur et al.   MARINE BIOTECHNOLOGY

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

(2014) Wei-Hong Xie et al.   MARINE BIOTECHNOLOGY

The Challenge of Ecophysiological Biodiversity for Biotechnological Applications of Marine Microalgae

(2014) Lucia Barra et al.   Marine Drugs

Characterization of iron-responsive promoters in the marine diatom Phaeodactylum tricornutum

(2014) Ryo Yoshinaga et al.   Marine Genomics

Light acclimation in diatoms: From phenomenology to mechanisms

(2014) Christian Wilhelm et al.   Marine Genomics

A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis

(2014) Kentaro Ifuku et al.   PHOTOSYNTHESIS RESEARCH

Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis

(2014) Philip D. Weyman et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals

(2014) Elodie Mathieu-Rivet et al.   Frontiers in Plant Science

Highly Efficient Transformation of the DiatomPhaeodactylum tricornutumby Multi-Pulse Electroporation

(2013) Mado MIYAHARA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatomPhaeodactylum tricornutum

(2013) Yangmin Gong et al.   FEBS LETTERS

Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum

(2013) Ying-Fang Niu et al.   Marine Drugs

High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation

(2013) Chunye Zhang et al.   Marine Genomics

Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

(2013) Mary L. Hamilton et al.   METABOLIC ENGINEERING

AUREOCHROME1a-Mediated Induction of the Diatom-Specific CyclindsCYC2Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)

(2013) Marie J.J. Huysman et al.   PLANT CELL

Molecular and Photosynthetic Responses to Prolonged Darkness and Subsequent Acclimation to Re-Illumination in the Diatom Phaeodactylum tricornutum

(2013) Marianne Nymark et al.   PLoS One

Aureochrome 1a Is Involved in the Photoacclimation of the Diatom Phaeodactylum tricornutum

(2013) Benjamin Schellenberger Costa et al.   PLoS One

Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth

(2013) E. M. Trentacoste et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetic engineering of algal chloroplasts: Progress and prospects

(2013) S. Purton et al.   RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

Transformation of diatom Phaeodactylum tricornutum by electroporation and establishment of inducible selection marker

(2012) Ying-Fang Niu et al.   BIOTECHNIQUES

Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum

(2012) M. Dambek et al.   JOURNAL OF EXPERIMENTAL BOTANY

Establishment of a Genetic Transformation System for the Marine Pennate Diatom Fistulifera sp. Strain JPCC DA0580—A High Triglyceride Producer

(2012) Masaki Muto et al.   MARINE BIOTECHNOLOGY

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency

(2012) Franziska Hempel et al.   Microbial Cell Factories

The role of C4metabolism in the marine diatomPhaeodactylum tricornutum

(2012) Maya Haimovich-Dayan et al.   NEW PHYTOLOGIST

Silencing of the Violaxanthin De-Epoxidase Gene in the Diatom Phaeodactylum tricornutum Reduces Diatoxanthin Synthesis and Non-Photochemical Quenching

(2012) Johann Lavaud et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

From Fields to Fuels: Recent Advances in the Microbial Production of Biofuels

(2012) Yan Kung et al.   ACS Synthetic Biology

The place of diatoms in the biofuels industry

(2012) Mark Hildebrand et al.   Biofuels-UK

Meganucleases and Other Tools for Targeted Genome Engineering: Perspectives and Challenges for Gene Therapy

(2011) George Silva et al.   CURRENT GENE THERAPY

Cloning and molecular characterization of a novel acyl-CoA:diacylglycerol acyltransferase 1-like gene (PtDGAT1) from the diatom Phaeodactylum tricornutum

(2011) Freddy Guihéneuf et al.   FEBS Journal

Carotenoids in Algae: Distributions, Biosyntheses and Functions

(2011) Shinichi Takaichi   Marine Drugs

Microalgae as bioreactors for bioplastic production

(2011) Franziska Hempel et al.   Microbial Cell Factories

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum

(2011) Franziska Hempel et al.   PLoS One

What’s so special about model organisms?

(2011) Rachel A. Ankeny et al.   STUDIES IN HISTORY AND PHILOSOPHY OF SCIENCE

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

Genetic Engineering of Algae for Enhanced Biofuel Production

(2010) Randor Radakovits et al.   EUKARYOTIC CELL

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum

(2010) Randor Radakovits et al.   METABOLIC ENGINEERING

Evidence for the Existence of One Antenna-Associated, Lipid-Dissolved and Two Protein-Bound Pools of Diadinoxanthin Cycle Pigments in Diatoms

(2010) B. Lepetit et al.   PLANT PHYSIOLOGY

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light

(2010) B. Bailleul et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

FIRST INDUCED PLASTID GENOME MUTATIONS IN AN ALGA WITH SECONDARY PLASTIDS: psbA MUTATIONS IN THE DIATOM PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE) REVEAL CONSEQUENCES ON THE REGULATION OF PHOTOSYNTHESIS1

(2009) Arne C. Materna et al.   JOURNAL OF PHYCOLOGY

Gene silencing in the marine diatom Phaeodactylum tricornutum

(2009) Valentina De Riso et al.   NUCLEIC ACIDS RESEARCH

Diatoms in biotechnology: modern tools and applications

(2008) Andrew Bozarth et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of gene expression system in a marine diatom using viral promoters of a wide variety of origin

(2008) Kunihiro Sakaue et al.   PHYSIOLOGIA PLANTARUM