Article
Environmental Sciences
Xiao-peng Lu, Jia-huan Liu, Xiang-yun Fu, Feng-jin Wang, Hua Wu, Hua Weng, Zhi-qing Ma
Summary: The compound wilforine from Tripterygium wilfordii shows excellent insecticidal activity against Mythimna separata. Study on the plasma membrane calcium transporting ATPase (PMCA) and inositol 1,4,5-trisphosphate receptor (IP3R) of M. separata reveals high homology with insects and potential novel targets for insecticides. Silencing the PMCA gene decreases susceptibility to wilforine, indicating PMCA as a possible target, while silencing the IP3R gene does not affect susceptibility, suggesting wilforine may not interact with IP3R.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Food Science & Technology
Gabriela Cristina Soares Rodrigues, Mayara dos Santos Maia, Andreza Barbosa Silva Cavalcanti, Renata Priscila Costa Barros, Luciana Scotti, Carlos L. Cespedes-Acuna, Eugene N. Muratov, Marcus Tullius Scotti
Summary: Pesticides are commonly used to control insects and pests, but the overuse can have negative effects, leading to the search for natural alternatives. This study aimed to identify natural products with insecticidal potential against specific pests, utilizing QSAR models and molecular docking to prioritize promising compounds.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wenliang Zhang, Jingjing Chen, Xiaohua Du
Summary: In order to combat the increase in insecticide resistance, researchers designed and synthesized a series of novel 2-phenylpyridine derivatives containing N-phenylbenzamide moieties. The compounds were easily separated with mild reaction conditions, yielding approximately 85%. The structures were characterized using NMR spectroscopy and HRMS, and the insecticidal activity was analyzed using the leaf dipping method. Five compounds exhibited 100% inhibition against Mythimna separata at a concentration of 500 mg/L, indicating the potential of 2-phenylpyridine moieties in the discovery of novel and effective insecticides.
Article
Multidisciplinary Sciences
Chao Xu, Jichao Ji, Xiangzhen Zhu, Ningbo Huangfu, Hui Xue, Li Wang, Kaixin Zhang, Dongyang Li, Lin Niu, Ran Chen, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: A high-quality chromosome-level genome assembly of the oriental armyworm, Mythimna separata, was successfully achieved in this study. This genome assembly provides a significant genetic resource for future studies of this insect and contributes to the development of management strategies.
Article
Environmental Sciences
Shujie Ma, Ting Ma, Meiru Ren, Hai Li, Zhiqing Ma
Summary: The study investigated the molecular mechanism of wilforine in Mythimna separata through transcriptome analysis and RNA interference, revealing its impact on genes related to calcium signaling pathway and muscle contraction. Downregulation of the Ryanodine receptor gene by wilforine led to inhibited growth and development, reducing susceptibility to the insecticide. Dysfunction of the RyR Ca2+ release channel was proposed as a significant lethal mechanism of wilforine based on previous toxic symptoms and muscle tissue lesions studies.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Saif ul Malook, Yuxing Xu, Jinfeng Qi, Jing Li, Lei Wang, Jianqiang Wu
Summary: This study investigated the priming induction in maize systemic leaves by herbivory, showing that both duration of simulated herbivory and perception of insect oral secretions play important roles in activating primed defenses in systemic leaves. This research sheds new light on the regulation and ecological function of priming in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Entomology
Chao Xu, Junyu Luo, Li Wang, Xiangzhen Zhu, Hui Xue, Ningbo Huangfu, Xueke Gao, Dongyang Li, Kaixin Zhang, Ran Chen, Jichao Ji, Changying Niu, Jinjie Cui
Summary: This study reveals that exposure to insect-resistant transgenic maize reduces gut bacterial diversity and alters the gene expression of M. separata. Furthermore, Enterobacter cloacae and Enterococcus mundtii enhance the susceptibility of M. separata to Bt toxin.
JOURNAL OF PEST SCIENCE
(2023)
Article
Plant Sciences
Dong-jiang Lin, Yong Fang, Ling-yun Li, Li-zhao Zhang, San-ji Gao, Ran Wang, Jin-da Wang
Summary: The oriental armyworm Mythimna separata is a significant agricultural pest that can feed on the leaves of many crops, causing extensive damage and severe losses. This study found that chlorogenic acid has a significant lethal effect on the larvae of M. separata, and the mortality increases with higher concentrations of chlorogenic acid. Additionally, chlorogenic acid at a sublethal concentration affects the growth and development of the larvae, reducing their survival rate, pupation rate, eclosion rate, sex ratio, and oviposition amount. Transcriptome analysis revealed that several MsCYP450 genes are up-regulated in response to chlorogenic acid, and the dietary ingestion of dsMsCYP450 reduces the mRNA level of these genes and increases mortality in the presence of chlorogenic acid.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Entomology
Aneela Younas, Muhammad Irfan Waris, Muhammad Shaaban, Muhammad Tahir ul Qamar, Man-Qun Wang
Summary: The study revealed that MsepCSP14 shows significant expression and high binding affinity in female Mythimna separata. This chemosensory protein is likely to mediate chemosensory functions in M. separata.
Article
Biochemistry & Molecular Biology
Qiuyuan Zhang, Yicheng Zhou, Yunfei Li, Bahar Ali, Zhihui Zhu
Summary: Toxin-antitoxin systems were characterized in insect cells, revealing high toxicity of the toxins and limited toxicity neutralization by the antitoxins. Both toxins induced apoptosis and necrosis in the cells, while the antitoxins partially neutralized their toxicity in a dose-dependent manner. The MazF-MazE system showed promising potential for application in insects, such as for developing new sterile insect technique (SIT).
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Food Science & Technology
Yueqin Wang, Jing Yang, Tiantao Zhang, Shuxiong Bai, Zhenying Wang, Kanglai He
Summary: This study investigates the mode of inheritance and fitness costs associated with resistance to Vip3Aa19 in a laboratory selected Mythimna separata strain. The results suggest that the resistance is inherited as a maternal and multigenic trait, and has significant fitness costs.
Article
Food Science & Technology
Yudong Quan, Jing Yang, Yueqin Wang, Patricia Hernandez-Martinez, Juan Ferre, Kanglai He
Summary: Laboratory selection can help understand the potential and mechanisms of insect populations' resistance, showing that a field population of Mythimna separata rapidly evolved high levels of resistance to Vip3Aa but not to Cry1Ab or Cry1F. Studies on binding mechanisms did not reveal differences between susceptible and resistant populations, suggesting altered binding may not be the main resistance mechanism.
Article
Entomology
Xiang-Wen Li, Li-Xiao Du, Lei Zhang, Yu-Fa Peng, Hong-Xia Hua, Jorg Romeis, Yun-He Li
Summary: The study showed that damage by Mythimna separata caterpillars on Bt corn plants reduced the fitness of Rhopalosiphum maidis aphids, leading to lower population growth rates. Aphids performed better on Bt corn when plants were infested with M. separata caterpillars. Therefore, reduced caterpillar infestation could benefit aphid development in Bt corn, which may be commercialized in China in the near future, necessitating monitoring of aphids and other non-target pests in farming fields.
Article
Physiology
Hong-Jia Yang, Meng-Yao Cui, Xiao-Hui Zhao, Chun-Yu Zhang, Yu-Shuo Hu, Dong Fan
Summary: In this study, the Trehalose-6-phosphate synthase (TPS) gene in Mythimna separata (MsTPS) was cloned and its expression patterns in different developmental stages and tissues were analyzed. The inhibition of MsTPS expression resulted in decreased trehalose content and changed expression of chitin synthase genes. It also had negative effects on M. separata larvae, including decreased weight, feeding capacity, and increased mortality and malformation rates.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Entomology
Kakeru Yokoi, Seiichi Furukawa, Rui Zhou, Akiya Jouraku, Hidemasa Bono
Summary: In this study, a reference genome of the oriental armyworm was constructed, and the quality of the genome was found to be sufficient for use as a reference. The number of predicted genes and coding sites were also determined. Through functional gene annotation, important genes such as the Toll and Immune Deficiency pathways and C-type lectins were identified. These research findings will contribute to molecular and genome-level research on the oriental armyworm and lepidopteran insects.