Article
Environmental Sciences
Xiao-peng Lu, Jia-huan Liu, Xiang-yun Fu, Feng-jin Wang, Hua Wu, Hua Weng, Zhi-qing Ma
Summary: The compound wilforine from Tripterygium wilfordii shows excellent insecticidal activity against Mythimna separata. Study on the plasma membrane calcium transporting ATPase (PMCA) and inositol 1,4,5-trisphosphate receptor (IP3R) of M. separata reveals high homology with insects and potential novel targets for insecticides. Silencing the PMCA gene decreases susceptibility to wilforine, indicating PMCA as a possible target, while silencing the IP3R gene does not affect susceptibility, suggesting wilforine may not interact with IP3R.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Physiology
Hong-Jia Yang, Meng-Yao Cui, Xiao-Hui Zhao, Chun-Yu Zhang, Yu-Shuo Hu, Dong Fan
Summary: In this study, the Trehalose-6-phosphate synthase (TPS) gene in Mythimna separata (MsTPS) was cloned and its expression patterns in different developmental stages and tissues were analyzed. The inhibition of MsTPS expression resulted in decreased trehalose content and changed expression of chitin synthase genes. It also had negative effects on M. separata larvae, including decreased weight, feeding capacity, and increased mortality and malformation rates.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yan-fang Guo, Jia-ren Qiu, Tao Chen, San-ji Gao, Bu Su-hong, Ran Wang, Jin-da Wang
Summary: The study identified that the MsOA2B2 gene in Mythimna separata has different expression levels in various developmental stages, with higher expression in egg stages and the second and fourth instars. Further analysis revealed that the gene is expressed at a higher level in the head of sixth instar larvae. Additionally, dietary ingestion of dsMsOA2B2 led to reduced expression levels of MsOA2B2 and decreased mortality following amitraz treatment.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Meimei Li, Boliao Li, Qi Yang, Yanying Li, Junxiang Wu, Xiangli Xu
Summary: In this study, we identified 50 neuropeptides including neuropeptide Y (NPY) of Mythimna separata using transcriptome sequencing. The spatial and temporal expression profile of NPY indicated its important role in feeding regulation and energy metabolism. Knockdown of NPY significantly inhibited food uptake and body weight, delayed developmental duration, and altered energy storage in M. separata larvae.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Entomology
Fuyuan Li, Gaoliang Xing, Yixuan Li, Peng Chen, Qin Hu, Ming Chen, Yiyu Li, Haiqun Cao, Yong Huang
Summary: In this study, 79 cuticular protein (CP) genes were identified from the larval integuments of Mythimna separata. Among them, 57 genes belonged to the family with conserved Rebers & Riddiford (R&R) consensus (CPR family). RT-qPCR analysis showed that most RR-1 genes were highly expressed in larval stages and integuments. RNAi experiments revealed that knockdown of MsCPR2 resulted in abnormal integument formation, while knockdown of MsCPR3 and MsCPR4 led to molting failure. Therefore, this study provides new insights into the expression and function of RR-1 genes in the development of integuments in M. separata.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Mei-Mei Li, Qi Yang, Li-Hui Chen, Yan-Ying Li, Jun-Xiang Wu, Xiang-Li Xu
Summary: The study identified and cloned sNPF and its receptors from Mythimna separata, an infamous pest that threatens cereal crops. It was found that sNPF signaling plays a vital role in the feeding regulation of this pest, suggesting it as a potential target for pest control.
Article
Plant Sciences
Dong-jiang Lin, Yong Fang, Ling-yun Li, Li-zhao Zhang, San-ji Gao, Ran Wang, Jin-da Wang
Summary: The oriental armyworm Mythimna separata is a significant agricultural pest that can feed on the leaves of many crops, causing extensive damage and severe losses. This study found that chlorogenic acid has a significant lethal effect on the larvae of M. separata, and the mortality increases with higher concentrations of chlorogenic acid. Additionally, chlorogenic acid at a sublethal concentration affects the growth and development of the larvae, reducing their survival rate, pupation rate, eclosion rate, sex ratio, and oviposition amount. Transcriptome analysis revealed that several MsCYP450 genes are up-regulated in response to chlorogenic acid, and the dietary ingestion of dsMsCYP450 reduces the mRNA level of these genes and increases mortality in the presence of chlorogenic acid.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Ya-ru Wang, Jia-song Zhang, Ran Wang, You-ming Hou, Hu-aying Fu, Yuan Xie, San-ji Gao, Jin-da Wang
Summary: The study investigated changes in gene expression and metabolic processes in sugarcane plants fed on by Mythimna separata larvae for 12 and 24 hours through transcriptome and metabolomic analysis. A total of 13,662 genes and 55 metabolites were found to be differentially regulated in sugarcane plants under attack. The activation of genes related to phytohormones, transcription factors, and kinases, as well as the regulation of metabolism compounds like carbohydrates, amino acids, and ferulic substances, were observed in sugarcane defense response against herbivorous insects.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Dong-Jiang Lin, Ya-Xin Zhang, Yong Fang, San-Ji Gao, Ran Wang, Jin-Da Wang
Summary: Chlorogenic acid is a potential botanical insecticide that naturally occurs in plants and can control the armyworm Mythimna separata. Proteomic analysis of saliva from M. separata exposed to chlorogenic acid revealed the enrichment of proteins involved in glutathione metabolism and the pentose phosphate pathway. Furthermore, carboxylesterase proteins were found to be highly expressed and associated with increased enzyme activity and susceptibility to chlorogenic acid.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Chao Xu, Jichao Ji, Xiangzhen Zhu, Ningbo Huangfu, Hui Xue, Li Wang, Kaixin Zhang, Dongyang Li, Lin Niu, Ran Chen, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: A high-quality chromosome-level genome assembly of the oriental armyworm, Mythimna separata, was successfully achieved in this study. This genome assembly provides a significant genetic resource for future studies of this insect and contributes to the development of management strategies.
Article
Plant Sciences
Saif ul Malook, Yuxing Xu, Jinfeng Qi, Jing Li, Lei Wang, Jianqiang Wu
Summary: This study investigated the priming induction in maize systemic leaves by herbivory, showing that both duration of simulated herbivory and perception of insect oral secretions play important roles in activating primed defenses in systemic leaves. This research sheds new light on the regulation and ecological function of priming in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Physiology
Wen-Bo Chen, Li-Xiao Du, Xiao-Yan Gao, Long-Long Sun, Lin-Lin Chen, Gui-Ying Xie, Shi-Heng An, Xin-Cheng Zhao
Summary: A large number of chemosensory genes have been identified in the peripheral sensory organs of Mythimna separata, and their expression in various tissues, including the brain, was examined. The results showed sex- and tissue-specific expression of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in the brain, and most of them could also be detected in the antennae. The findings expand our understanding of the expression patterns of OBPs and CSPs in sensory and non-sensory tissues, providing valuable reference data for exploring their functions and developing pest control strategies.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenbo Chen, Xiaoyan Gao, Huixin Wang, Guiying Xie, Shiheng An, Yongkun Du, Xincheng Zhao
Summary: This study provides a comprehensive characterization of the 5-HT7 receptors in the oriental armyworm, an important agricultural pest. Two molecularly distinct isoforms of the 5-HT7 receptor were identified, and both isoforms were activated by 5-HT and synthetic agonists. The isoforms showed differential expression in the brain and chemosensory organs. These findings offer important insights for further understanding the function of the 5-HT7 receptors and may have implications for the development of new pesticides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Sibo Wang, Hongjia Yang, Yushuo Hu, Chunyu Zhang, Dong Fan
Summary: High-density environment of Mythimna separata leads to melanization and overeating behavior, causing serious damage to crops. This study investigated the impact of population density on M. separata through transcriptome and metabolome analysis, revealing the molecular mechanism of phase change and overeating behavior under high population density. The insulin-like signaling pathway plays a crucial role in this process.
Article
Entomology
Tingting Chen, Xue Zhou, Jing Wu, Gaoliang Xing, Haiqun Cao, Yong Huang
Summary: The function of CSPs in the development of Mythimna separata larvae was investigated. Two CSP genes, MsCSP17 and MsCSP18, were identified with different expression patterns among developmental stages and tissues. Both genes were highly expressed in larval head, thorax, integument, and mandible.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2023)