Article
Multidisciplinary Sciences
Christopher Thomas, Benjamin Wetherall, Mark D. Levasseur, Rebecca J. Harris, Scott T. Kerridge, Jonathan M. G. Higgins, Owen R. Davies, Suzanne Madgwick
Summary: The study reveals an excess of securin over separase in mouse oocytes during meiosis I, with a mechanism promoting securin destruction in prometaphase I. This destruction mechanism relies on specific residues within securin that are exposed when not bound to separase. The authors suggest that this mechanism is crucial for successful meiotic progression in mouse oocytes by ensuring the removal of non-separase-bound securin before metaphase.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Guanying Li, Xunwu Hu, Xia Wu, Ye Zhang
Summary: By synthesizing peptide-based rotor molecules with the potential to self-assemble, researchers were able to target microtubules and inhibit cancer cell proliferation without inducing obvious neurotoxicity, showcasing a novel approach for cancer therapy.
Article
Food Science & Technology
Cong Ma, Zhuonan Yang, Shouxin Zhang, Xueke Zhang, Siyuan Wang, Huiru Cheng, Yang Liu, Hongzhen Ruan, Zuying Xu, Chunmei Liang, Dan Liang, Zhiming Ding, Yajing Liu, Yunxia Cao
Summary: CBZ exposure negatively affects the maturation of mouse oocytes, leading to disruptions in polar body extrusion, cell cycle distribution, spindle assembly, chromosomal alignment, and kinetochore microtubule attachment.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Felipe Mora-Bermudez, Philipp Kanis, Dominik Macak, Jula Peters, Ronald Naumann, Lei Xing, Mihail Sarov, Sylke Winkler, Christina Eugster Oegema, Christiane Haffner, Pauline Wimberger, Stephan Riesenberg, Tomislav Maricic, Wieland B. Huttner, Svante Paabo
Summary: Since the separation of modern humans from Neanderthals, around 100 amino acid substitutions have spread to almost all modern humans. Research has shown that certain modern human-specific amino acid substitutions can improve the accuracy of chromosome segregation in stem cells of the developing neocortex.
Article
Biochemistry & Molecular Biology
Da-Jie Deng, Xi Wang, Kai-Ye Yue, Yamei Wang, Quan-Wen Jin
Summary: This study investigated the role of fission yeast PP2A in SAC silencing. The results showed that the absence of two B56 regulatory subunits of PP2A only slightly delayed SAC inactivation. Overexpression of individual PP2A subunits could only slightly suppress the SAC silencing defects in PP1 deletion cells. Therefore, fission yeast PP2A is not a key regulator actively involved in SAC inactivation.
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Xiao-Han Li, Meng-Hao Pan, Yi Xu, Yao Xu, Ming-Hong Sun, Shao-Chen Sun
Summary: Mps1 plays crucial roles in regulating the spindle assembly checkpoint and DNA repair during mouse early embryo development. Loss of Mps1 activity leads to abnormal early cleavage, disrupted spindle organization, increased DNA damage, oxidative stress, and activation of apoptosis and autophagy pathways.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Cell Biology
Stanislau Yatskevich, Jessie S. Kroonen, Claudio Alfieri, Thomas Tischer, Anna C. Howes, Linda Clijsters, Jing Yang, Ziguo Zhang, Kaige Yan, Alfred C. O. Vertegaal, David Barford
Summary: SUMOylation of the APC/C rearranges the structure of the WHB domain of the cullin subunit, leading to reduced affinity of APC/C-Cdc20 for the mitotic checkpoint complex and allowing for more efficient ubiquitination of substrates in the presence of the MCC, contributing to timely anaphase onset.
Article
Cell Biology
Wonkyung Oh, Ting Ting Wu, Seo-Yeon Jeong, Ho Jin You, Jung-Hee Lee
Summary: CtIP plays a critical role in regulating mitosis by interacting with TPX2 to control spindle dynamics and regulate the concentration of Aurora A and microtubule intensity at the spindle poles. Depletion of CtIP may lead to improper execution of mitosis and result in chromosomal instability.
Article
Reproductive Biology
Jie Zhang, Hong-Jie Yuan, Jiang Zhu, Shuai Gong, Ming-Jiu Luo, Jing-He Tan
Summary: Topoisomerase II dysfunction impairs chromatin condensation and chromosome alignment, activates Aurora B, and leads to MI arrest and PB1 abscission failure.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Ana C. Henriques, Patricia M. A. Silva, Bruno Sarmento, Hassan Bousbaa
Summary: Antimitotic drugs can induce cell death by activating the spindle assembly checkpoint, but cancer cells treated with these drugs can escape death through mitotic slippage. By inhibiting SAC silencing and delaying mitotic slippage, the effectiveness of antimitotic drugs can be improved, leading to enhanced cell death. Inhibition of SAC silencing also sensitizes cancer cells to apoptosis-inducing small molecules, suggesting a potential therapeutic strategy for antimitotic therapies.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Aleksandar Mihajlovic, Candice Byers, Laura Reinholdt, Greg Fitzharris
Summary: Chromosome segregation errors in mammalian oocyte meiosis, including SAC ineffectiveness in meiosis-II, contribute to age-related aneuploidy.
Article
Biochemistry & Molecular Biology
Tobias Raisch, Giuseppe Ciossani, Ennio D'Amico, Verena Cmentowski, Sara Carmignani, Stefano Maffini, Felipe Merino, Sabine Wohlgemuth, Ingrid R. Vetter, Stefan Raunser, Andrea Musacchio
Summary: A high-resolution cryo-EM structure of the ROD-Zwilch-ZW10 complex reveals essential features, such as a farnesyl-binding site required for Spindly binding. In vitro assays demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly complex, and the molecular mechanism of phosphorylation-dependent filament nucleation is described.
Article
Biochemistry & Molecular Biology
Jian Liu, Hongsheng Xue, Chunxiao Li, Xiangyu Chen, Jiannan Yao, Dongkui Xu, Haili Qian
Summary: We identified the subcellular distribution of MTA1 in mitotic cancer cells and its role in inhibiting SAC activation. MTA1 is localized in the spindle apparatus throughout mitosis and interacts with TPR. The MTA1-TPR complex represents a distinct SAC-associated complex separate from the TPR-MAD1/MAD2 complex.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Wei Yue, Yue Wang, Tie-Gang Meng, Hong-Yong Zhang, Xin-Ran Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study investigates the role of outer kinetochore protein KNL1 in mouse oocytes and reveals its critical function in ensuring timely and accurate chromosome segregation during oocyte meiotic maturation.
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.