Article
Chemistry, Analytical
In Young Kim, Jae Won Choi, Ik Hwan Kwon, Seonae Hwangbo, Song-Hwa Bae, Minjeong Kwak, Jaeseok Kim, Tae Geol Lee, Min Beom Heo
Summary: In this study, the cytotoxicity of SiO2 nanoparticles was compared in different cell culture systems and scaffold materials. The results showed that the cytotoxicity of SiO2 nanoparticles can be influenced by both the culture system and scaffold type.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Multidisciplinary
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Summary: The study discusses the importance of vascularization techniques in tissue engineering and the current research progress. By using different strategies and materials, more biologically relevant and organ-specific 3D models can be established, which are expected to be used for regenerative medicine and drug development in the future.
Article
Cell Biology
Jeremy Dufau, Joanne X. Shen, Morgane Couchet, Thais De Castro Barbosa, Niklas Mejhert, Lucas Massier, Elena Griseti, Etienne Mouisel, Ez-Zoubir Amri, Volker M. Lauschke, Mikael Ryden, Dominique Langin
Summary: Adipocytes play diverse roles in physiology and pathology, with white, beige, and brown adipocytes differing in their lipid handling and thermogenic capacity. Cultured differentiated preadipocyte cell lines and primary mature adipocytes are used to mimic different types of adipocytes, but challenges remain in culture conditions and limitations. Advances in three-dimensional culture, such as adipose spheroids, provide a more in vivo-like model for studying adipocyte function and morphology.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Bangheng Liu, Min Jin, Dong-An Wang
Summary: A porous alginate hydrogel-based 3D culture system is effective in maintaining the phenotype and renewability of hematopoietic stem cells (HSCs). It outperforms traditional suspension or non-porous alginate hydrogel culture in terms of HSC expansion efficiency.
ACTA BIOMATERIALIA
(2023)
Article
Biochemical Research Methods
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Toerne, Stefanie M. Hauck, Matthias Meier
Summary: This study developed a microfluidic chip platform to simulate adipose tissue in vitro and revealed the differences in adipocytes under low/high glucose conditions using proteomics analysis. This in vitro model can be used to investigate the molecular and functional mechanisms of adipose tissue under normal and pathological conditions.
Article
Biotechnology & Applied Microbiology
Ho-Joon Lee, Seon Ju Mun, Cho-Rok Jung, Hyun-Mi Kang, Jae-Eun Kwon, Jae-Sung Ryu, Hyo-Suk Ahn, Ok-Seon Kwon, Jiwon Ahn, Kyung-Sik Moon, Myung Jin Son, Kyung-Sook Chung
Summary: This study established and characterized a novel human hepatic stellate cell line (LSC-1) and used it in various three-dimensional co-culture systems with differentiated HepaRG cells. Comparisons with a commercially available HSC line on monolayer culture showed that LSC-1 exhibited upregulation of fibrogenic genes and increased levels of matrix and adhesion proteins. LSC-1 also had positive effects on co-cultured differentiated HepaRG and showed enhanced responsiveness to transforming growth factor beta 1 exposure in 3D spheroid culture.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Yufei He, Zhuokai Liang, Jing Wang, Haojing Tang, Jian Li, Junrong Cai, Yunjun Liao
Summary: Excess and dysfunctional adipose tissue plays an important role in metabolic diseases. This study describes a novel ceiling culture-based model of human mature white adipocytes that can transdifferentiate into beige adipocytes. The use of rosiglitazone and rapamycin can modulate transdifferentiation and enhance the expression of beige adipocyte-specific genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chikahiro Imashiro, Kai Yamasaki, Ryu-ichiro Tanaka, Yusuke Tobe, Katsuhisa Sakaguchi, Tatsuya Shimizu
Summary: This study introduces a novel system that enhances transportation in cell tissues through diffusion and convection, improving tissue viability and promoting glucose consumption, lactate production, and oxygen transport. The proposed system potentially sets a new standard for 3D cell cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Meaghan E. Harley-Troxell, Madhu Dhar
Summary: Neural injuries disrupt normal nervous system function, but neurospheres have the potential to advance regenerative medicine and neural tissue engineering. By optimizing conditions, we successfully developed primary neural cell-derived neurospheres using a stress-free, 3D bioreactor, providing a necessary baseline for future studies and therapeutic use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Vaishali Chaurasiya, Dan Duc Pham, Jukka Harju, Anne Juuti, Anne Penttila, Sharath Kumar Goud Emmagouni, Van Dien Nguyen, Birong Zhang, Sanni Perttunen, Salla Keskitalo, You Zhou, Kirsi H. Pietilainen, P. A. Nidhina Haridas, Vesa M. Olkkonen
Summary: Communication between adipocytes and endothelial cells plays an important role in the metabolic function of white adipose tissue. This study developed a method to isolate adipose microvascular endothelial cells (AMvEC) from visceral adipose tissue (VAT) and established a co-culture system to study their communication. Various experiments validated the identity and functionality of the isolated cells. Additionally, the co-culture system can be used for drug screening.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Serena Calamaio, Marialaura Serzanti, Jennifer Boniotti, Annamaria Fra, Emirena Garrafa, Manuela Cominelli, Rosanna Verardi, Pietro Luigi Poliani, Silvia Dotti, Riccardo Villa, Giovanna Mazzoleni, Patrizia Dell'Era, Nathalie Steimberg
Summary: The process of developing a new drug is time-consuming and expensive, and hepatotoxicity is a major concern. Animal models have limitations due to interspecies differences, but human induced pluripotent stem cells (hiPSCs) offer new possibilities for drug testing. We miniaturized the process of generating hepatic organoids using hiPSC-derived mesenchymal stem cells, hepatocytes, and endothelial cells. These organoids were cultivated in vitro using static and dynamic cultures, and could be maintained for up to 1 week. This hiPSC-derived liver organoid model could potentially replace animal testing in pharmacological and toxicological studies.
Article
Multidisciplinary Sciences
Janith Wanigasekara, Lara F. Carroll, Patrick Cullen, Brijesh Tiwari, James Curtin
Summary: Three-dimensional (3D) cell culture models can effectively simulate the natural in vivo environment, shape, tissue stiffness, stressors, gradients, and cellular response, bridging the gap between in vitro cell cultures and in vivo responses, without the associated costs and ethical concerns of animal models.
Review
Oncology
Unnati Modi, Pooja Makwana, Rajesh Vasita
Summary: The review focuses on the role of molecular cross-talk between stromal cells and cancer cells in promoting metastasis through 3D cancer spheroid co-culture in vitro platform. This state-of-art approach highlights the potential for exploring effective treatments and personalized medicines to combat cancer metastasis and progression.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2021)
Article
Cell & Tissue Engineering
Takayoshi Oba, Satoshi Okamoto, Yasuharu Ueno, Megumi Matsuo, Tomomi Tadokoro, Shinji Kobayashi, Kazunori Yasumura, Shintaro Kagimoto, Yutaka Inaba, Hideki Taniguchi
Summary: In this study, a novel in vitro scaffold-free method for elastic cartilage reconstruction using human auricular perichondrial chondroprogenitor cells was developed. Inducing chondrogenesis with microscopic spheroids increased the chondrogenic potential. Craniofacial transplantation in immunodeficient mice showed that the reconstructed tissue was morphologically stable.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Pharmacology & Pharmacy
Sigrid A. Langhans
Summary: There has been a rapid increase in 3D cell culture technologies in recent years aimed at closely mimicking in vivo physiology. Each 3D cell culture system has its own strengths and weaknesses when it comes to in vivo tumor growth and the tumor microenvironment, which requires careful consideration when choosing a system for drug discovery based on factors like drug target and tumor origin.
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Review
Endocrinology & Metabolism
Margo P. Emont, Hui Yu, Jun Wu
JOURNAL OF ENDOCRINOLOGY
(2015)
Article
Cell Biology
Margo P. Emont, Stelios Mantis, Jonathan H. Kahn, Michael Landeche, Xuan Han, Robert M. Sargis, Ronald N. Cohen
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Abdul Soofi, Katherine I. Wolf, Margo P. Emont, Nathan Qi, Gabriel Martinez-Santibanez, Edward Grimley, Wesam Ostwani, Gregory R. Dressler
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Endocrinology & Metabolism
Juan Jiang, Margo P. Emont, Heejin Jun, Xiaona Qiao, Jiling Liao, Dong-il Kim, Jun Wu
METABOLISM-CLINICAL AND EXPERIMENTAL
(2017)
Review
Biochemistry & Molecular Biology
Margo P. Emont, Dong-il Kim, Jun Wu
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2019)
Article
Biochemistry & Molecular Biology
Heejin Jun, Hui Yu, Jianke Gong, Juan Jiang, Xiaona Qiao, Eric Perkey, Dong-Il Kim, Margo P. Emont, Alexander G. Zestos, Jung-Sun Cho, Jianfeng Liu, Robert T. Kennedy, Ivan Maillard, X. Z. Shawn Xu, Jun Wu
Article
Multidisciplinary Sciences
Marcella K. Vaicik, Jill Thyboll Kortesmaa, Sofia Moverare-Skrtic, Jarkko Kortesmaa, Raija Soininen, Goran Bergstrom, Claes Ohlsson, Li Yen Chong, Bjorn Rozell, Margo Emont, Ronald N. Cohen, Eric M. Brey, Karl Tryggvason
Article
Cell Biology
Heejin Jun, Yingxu Ma, Yong Chen, Jianke Gong, Shanshan Liu, Jine Wang, Alexander J. Knights, Xiaona Qiao, Margo P. Emont, X. Z. Shawn Xu, Shingo Kajimura, Jun Wu
DEVELOPMENTAL CELL
(2020)
Article
Cell Biology
Gregory P. Westcott, Margo P. Emont, Jin Li, Christopher Jacobs, Linus Tsai, Evan D. Rosen
Summary: Recent studies have shown that visceral adipose tissue (VAT) is not derived from mesothelial cells as previously believed. The developmental origin and identity of adipocyte progenitor cells remain active areas of research.
Article
Multidisciplinary Sciences
Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori, Angelica Di Vincenzo, Anja M. Jorgensen, Hesam Dashti, Adam Stefek, Elizabeth McGonagle, Sophie Strobel, Samantha Laber, Saaket Agrawal, Gregory P. Westcott, Amrita Kar, Molly L. Veregge, Anton Gulko, Harini Srinivasan, Zachary Kramer, Eleanna De Filippis, Erin Merkel, Jennifer Ducie, Christopher G. Boyd, William Gourash, Anita Courcoulas, Samuel J. Lin, Bernard T. Lee, Donald Morris, Adam Tobias, Amit Khera, Melina Claussnitzer, Tune H. Pers, Antonio Giordano, Orr Ashenberg, Aviv Regev, Linus T. Tsai, Evan D. Rosen
Summary: White adipose tissue, once considered bland, is now seen as dynamic, plastic and involved in various biological processes. High-fat feeding and other metabolic stressors lead to significant changes in adipose tissue, linked to insulin resistance, dyslipidemia and type 2 diabetes. Research provides detailed cellular atlases of human and mouse adipose tissue, highlighting interactions between different cell types in lean and obese states.
Article
Cell Biology
Suraj J. Patel, Nan Liu, Sam Piaker, Anton Gulko, Maynara L. Andrade, Frankie D. Heyward, Tyler Sermersheim, Nufar Edinger, Harini Srinivasan, Margo P. Emont, Gregory P. Westcott, Jay Luther, Raymond T. Chung, Shuai Yan, Manju Kumari, Reeby Thomas, Yann Deleye, Andre Tchernof, Phillip J. White, Guido A. Baselli, Marica Meroni, Dario F. De Jesus, Rasheed Ahmad, Rohit N. Kulkarni, Luca Valenti, Linus Tsai, Evan D. Rosen
Summary: This study reveals the important role of hepatic interferon regulatory factor 3 (IRF3) in regulating glucose homeostasis through the transcription factor Ppp2r1b. Elimination or reduction of IRF3 protects against steatosis and dysglycemia in obese mice. The study also suggests that obesity-induced insulin resistance and the restoration of glucose homeostasis can be reversed by inhibiting IRF3 expression. In addition, the activation of liver IRF3 in obese humans is enhanced and can be improved after bariatric surgery.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Haopeng Xiao, Luiz H. M. Bozi, Yizhi Sun, Christopher L. Riley, Vivek M. Philip, Mandy Chen, Jiaming Li, Tian Zhang, Evanna L. Mills, Margo P. Emont, Wenfei Sun, Anita Reddy, Ryan Garrity, Jiani Long, Tobias Becher, Laura Potano Vitas, Dina Laznik-Bogoslavski, Martha Ordonez, Xinyue Liu, Xiong Chen, Yun Wang, Weihai Liu, Nhien Tran, Yitong Liu, Yang Zhang, Aaron M. Cypess, Andrew P. White, Yuchen He, Rebecca Deng, Heiko Schoder, Joao A. Paulo, Mark P. Jedrychowski, Alexander S. Banks, Yu-Hua Tseng, Paul Cohen, Linus T. Tsai, Evan D. Rosen, Samuel Klein, Maria Chondronikola, Fiona E. McAllister, Nick Van Bruggen, Edward L. Huttlin, Bruce M. Spiegelman, Gary A. Churchill, Steven P. Gygi, Edward T. Chouchani
Summary: By studying a genetically diverse cohort of mice, researchers have identified the functional architecture of brown adipose tissue (BAT) proteome and discovered key regulators of BAT. They also found proteins that are associated with protection from or sensitivity to metabolic diseases. These findings provide important insights into the conserved mechanisms of BAT regulation over metabolic physiology.
Correction
Multidisciplinary Sciences
Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori, Angelica Di Vincenzo, Anja M. Jorgensen, Hesam Dashti, Adam Stefek, Elizabeth McGonagle, Sophie Strobel, Samantha Laber, Saaket Agrawal, Gregory P. Westcott, Amrita Kar, Molly L. Veregge, Anton Gulko, Harini Srinivasan, Zachary Kramer, Eleanna De Filippis, Erin Merkel, Jennifer Ducie, Christopher G. Boyd, William Gourash, Anita Courcoulas, Samuel J. Lin, Bernard T. Lee, Donald Morris, Adam Tobias, Amit V. Khera, Melina Claussnitzer, Tune H. Pers, Antonio Giordano, Orr Ashenberg, Aviv Regev, Linus T. Tsai, Evan D. Rosen
Article
Endocrinology & Metabolism
Jiling Liao, Juan Jiang, Heejin Jun, Xiaona Qiao, Margo P. Emont, Dong-il Kim, Jun Wu