Journal
NANOTECHNOLOGY
Volume 28, Issue 13, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/aa5f63
Keywords
PLA; PLGA; nanoencapsulation; synthetic peptides; antibacterial activity; biopolymers
Funding
- Vicerrectoria de Investigacion y Extension (VIE) of Universidad Industrial de Santander
- Colombian government through COLCIENCIAS
- COLCIENCIAS [110265740828]
Ask authors/readers for more resources
Nanocarrier systems are currently being developed for peptide, protein and gene delivery to protect them in the blood circulation and in the gastrointestinal tract. Polylactic acid (PLA) and poly(lactic-co-glycolic) acid (PLGA) nanoparticles loaded with a new antimicrobial GIBIM-P5S9K peptide were obtained by the double emulsion solvent extraction/evaporation method. PLA-and PLGA-NPs were spherical with sizes between 300 and 400 nm for PLA and 200 and 300 nm for PLGA and <0.3 polydispersity index as determined by dynamic light scattering and scanning electron microscopy), having the zeta potential of >20 mV. The peptide-loading efficiency of PLA-NP and PLGA-NPs was 75% and 55%, respectively. PLA-and PLGA-NPs released around 50% of this peptide over 8 h. In 10% human sera the size of peptide loaded PLA-and PLGA-NPs increased between 25.2% and 39.3%, the PDI changed from 3.2 to 5.1 and the surface charge from -7.15 to 14.6 mV. Both peptide loaded PLA-and PLGA-NPs at 0.5 mu M peptide concentration inhibited the growth of Escherichia coli O157:H7 (E. coli O157:H7), methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas. aeruginosa (P. aeruginosa). In contrast, free peptide inhibited at 10 mu M but did not inhibit at 0.5 and 1 mu M. These PLA-and PLGA-NPs presented <10% hemolysis indicating that they are hemocompatible and promising for delivery and protection system of GIBIM-P5S9K peptide.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available