期刊
NANOTECHNOLOGY
卷 28, 期 13, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/aa5f63
关键词
PLA; PLGA; nanoencapsulation; synthetic peptides; antibacterial activity; biopolymers
资金
- Vicerrectoria de Investigacion y Extension (VIE) of Universidad Industrial de Santander
- Colombian government through COLCIENCIAS
- COLCIENCIAS [110265740828]
Nanocarrier systems are currently being developed for peptide, protein and gene delivery to protect them in the blood circulation and in the gastrointestinal tract. Polylactic acid (PLA) and poly(lactic-co-glycolic) acid (PLGA) nanoparticles loaded with a new antimicrobial GIBIM-P5S9K peptide were obtained by the double emulsion solvent extraction/evaporation method. PLA-and PLGA-NPs were spherical with sizes between 300 and 400 nm for PLA and 200 and 300 nm for PLGA and <0.3 polydispersity index as determined by dynamic light scattering and scanning electron microscopy), having the zeta potential of >20 mV. The peptide-loading efficiency of PLA-NP and PLGA-NPs was 75% and 55%, respectively. PLA-and PLGA-NPs released around 50% of this peptide over 8 h. In 10% human sera the size of peptide loaded PLA-and PLGA-NPs increased between 25.2% and 39.3%, the PDI changed from 3.2 to 5.1 and the surface charge from -7.15 to 14.6 mV. Both peptide loaded PLA-and PLGA-NPs at 0.5 mu M peptide concentration inhibited the growth of Escherichia coli O157:H7 (E. coli O157:H7), methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas. aeruginosa (P. aeruginosa). In contrast, free peptide inhibited at 10 mu M but did not inhibit at 0.5 and 1 mu M. These PLA-and PLGA-NPs presented <10% hemolysis indicating that they are hemocompatible and promising for delivery and protection system of GIBIM-P5S9K peptide.
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