Article
Cell Biology
Meghali Aich, Asgar Hussain Ansari, Li Ding, Vytautas Iesmantavicius, Deepanjan Paul, Chunaram Choudhary, Souvik Maiti, Frank Buchholz, Debojyoti Chakraborty
Summary: This study reveals that the lncRNA-associated protein TOBF1 regulates the alternative splicing of transcripts necessary for maintaining stem cell identity in mouse ESCs. One of the affected genes is serine/arginine splicing factor 1 (SRSF1), whose splicing leads to global changes in splicing and expression of downstream genes involved in the maintenance of ESC pluripotency. The study shows that TOBF1 chromatin occupancy, pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and splicing converge in specific nuclear territories, collectively contributing to the maintenance of mouse ESC identity.
Article
Biochemistry & Molecular Biology
Dong Keon Kim, Jang-Seok Lee, Eun Young Lee, Hansol Jang, Suji Han, Hee Yeon Kim, In-Young Hwang, Ji-Woong Choi, Hyun Mu Shin, Hye Jin You, Hong-Duk Youn, Hyonchol Jang
Summary: O-GlcNAcylation modification of Sox2 at the T258 site plays a critical role in regulating self-renewal and early cell fate in mouse ESCs, with mutations at this site leading to reduced self-renewal capabilities.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Lucy LeBlanc, Mijeong Kim, Aparna Kambhampati, Albert J. Son, Nereida Ramirez, Jonghwan Kim
Summary: Cell density impacts gene expression and differentiation in mouse embryonic stem cells; β-catenin is primarily at adherens junctions at high density but translocates to the nucleus at low density; Esrrb maintains pluripotency gene expression at high density and decreases DNA association at low density.
Review
Cell Biology
Puja Agrawal, Sridhar Rao
Summary: Cell fate decisions rely on gene expression changes mediated by regulatory elements like enhancers and CTCF binding sites. These elements play crucial roles in early mammalian development and impact cell-type specific gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yi Hao, Xiang Li, Ke Qin, Yujie Shi, Yanwen He, Che Zhang, Bo Cheng, Xiwen Zhang, Guangyu Hu, Shuyu Liang, Qi Tang, Xing Chen
Summary: The O-GlcNAc modification is found to affect the self-renewal and differentiation of embryonic stem cells (ESCs), and the core pluripotency transcription factor NANOG is regulated by this modification. During neuronal lineage differentiation, the O-GlcNAc modification decreases, and suppressing O-GlcNAcylation can upregulate neuron-related genes, thus influencing the fate determination of ESCs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Cell & Tissue Engineering
Martin Bergert, Sergio Lembo, Sumana Sharma, Luigi Russo, Danica Milovanovic, Kristjan H. Gretarsson, Mandy Boermel, Pierre A. Neveu, Jamie A. Hackett, Evangelia Petsalaki, Alba Diz-Munoz
Summary: Cell membrane mechanics play a crucial role in the exit from naive pluripotency of mouse embryonic stem cells, with a decrease in membrane-to-cortex attachment identified as an essential cell-intrinsic mechanism for differentiation.
Article
Biochemistry & Molecular Biology
Dan Li, Jihong Yang, Vikas Malik, Yuting Huang, Xin Huang, Hongwei Zhou, Jianlong Wang
Summary: RNA helicase eIF4A3 plays a crucial role in maintaining pluripotency of stem cells by post-transcriptionally controlling the cell cycle, particularly the efficient nuclear export of Ccnb1 mRNA.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell & Tissue Engineering
Laura Garcia-Prat, Kerstin B. Kaufmann, Florin Schneiter, Veronique Voisin, Alex Murison, Jocelyn Chen, Michelle Chan-Seng-Yue, Olga Gan, Jessica L. McLeod, Sabrina A. Smith, Michelle C. Shoong, Darrien Parris, Kristele Pan, Andy G. X. Zeng, Gabriela Krivdova, Kinam Gupta, Shin-Ichiro Takayanagi, Elvin Wagenblast, Weijia Wang, Mathieu Lupien, Timm Schroeder, Stephanie Z. Xie, John E. Dick
Summary: This study reveals that transcription factor EB-mediated control of lysosomal activity plays a central role in regulating the fate determination of stem cells. TFEB induces the endolysosomal pathway to limit LT-HSC activation, promoting quiescence and self-renewal, while MYC engages biosynthetic processes to drive stem cell activation.
Article
Cell Biology
Feng Yue, Stephanie N. Oprescu, Jiamin Qiu, Lijie Gu, Lijia Zhang, Jingjuan Chen, Naagarajan Narayanan, Meng Deng, Shihuan Kuang
Summary: In skeletal muscle satellite cells, the dynamic distribution of lipid droplets determines cell fate, with LDLow cells outperforming LDHigh cells in regeneration and self-renewal. Interventions in LD biogenesis and catabolism disrupt cell fate balance and impair the regenerative capacity of SCs.
Article
Cell & Tissue Engineering
Fuquan Chen, Xiaomin Li, Xiao Feng, Tingting Gao, Weiyu Zhang, Zhi Cheng, Xuan Zhao, Ruiqing Chen, Xinyi Lu
Summary: In this study, lncRNA Lx8-SINE B2 was identified as a novel regulator of ESC proliferation, cell cycle, and metabolism through working with Eno1.
Article
Biochemistry & Molecular Biology
Sofia Hu, Emily Metcalf, Dig Bijay Mahat, Lynette Chan, Noor Sohal, Meenakshi Chakraborty, Maxwell Hamilton, Arundeep Singh, Abhyudai Singh, Jacqueline A. Lees, Phillip A. Sharp, Salil Garg
Summary: This study characterizes the regulatory program underlying heterogeneity in murine embryonic stem cell (mESC) states, showing that antagonism between factors at enhancers results in gene expression heterogeneity and formation of cell states. The results suggest that the regulation of enhancer binding can dampen variation in gene expression and alter the kinetics of switching between states.
Review
Cell & Tissue Engineering
Heyao Zhang, Xuepeng Wang, Jingsheng Li, Ronghua Shi, Ying Ye
Summary: This review summarizes the functional and mechanistic studies of the BAF complex in mouse ESCs and early embryonic development. It highlights the discussion on the distinct phenotypes resulting from the deletion of different BAF subunits in ESCs and embryos.
STEM CELLS INTERNATIONAL
(2021)
Editorial Material
Biochemistry & Molecular Biology
Natalia Y. Martynova, Elena A. Parshina, Andrey G. Zaraisky
Summary: Zyxin, initially recognized as a minor actin cytoskeleton protein, not only regulates actin filaments assembly and repair but also plays a crucial role in gene expression and cell differentiation, particularly during embryogenesis process.
Article
Multidisciplinary Sciences
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Summary: Genome remethylation is crucial for mammalian development, with a study showing that deficiencies in Dnmt3a and Dnmt3b lead to ES cells developing into trophoblast cells due to failed suppression of Ascl2. Regulating the expression of Ascl2 can impact the transdifferentiation and contribution of ES cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell & Tissue Engineering
Ram Prosad Chakrabarty, Navdeep S. Chandel
Summary: Mitochondrial metabolism plays a crucial role in determining stem cell fate and function, beyond its traditional roles in ATP production and support for stem cell survival and growth. This review focuses on key conceptual ideas regarding how mitochondria control mammalian stem cell fate and function through mechanisms such as reactive oxygen species generation and TCA cycle metabolite production.
Article
Multidisciplinary Sciences
Wenxin Li, Yanwen Zhou, Xiaoqian Zhang, Ying Yang, Songsong Dan, Tong Su, Shiqi She, Weilai Dong, Qingwei Zhao, Jia Jia, Hangping Yao, Min Zheng, Bo Kang, Ying-Jie Wang
SCIENTIFIC REPORTS
(2017)
Article
Oncology
Chao Ye, Xiaoqian Zhang, Xinyu Chen, Qingyi Cao, Xiaobing Zhang, Yanwen Zhou, Wenxin Li, Liangjie Hong, Haiyang Xie, Xiaoli Liu, Hongcui Cao, Ying-Jie Wang, Bo Kang
Article
Medicine, Research & Experimental
Ning Zhou, Xiao-Bing Zhang, Cheng Chen, Xin-Yu Chen, Bo Kang, Jian-Qin He, Guo-Zhong Gong, Ying-Jie Wang, Yan-Wen Zhou
Article
Chemistry, Multidisciplinary
Anhao Tian, Bo Kang, Baizhou Li, Biying Qiu, Wenhong Jiang, Fangjie Shao, Qingqing Gao, Rui Liu, Chengwei Cai, Rui Jing, Wei Wang, Pengxiang Chen, Qinghui Liang, Lili Bao, Jianghong Man, Yan Wang, Yu Shi, Jin Li, Minmin Yang, Lisha Wang, Jianmin Zhang, Simon Hippenmeyer, Junming Zhu, Xiuwu Bian, Ying-Jie Wang, Chong Liu
Article
Multidisciplinary Sciences
Songsong Dan, Yuelin Song, Xiaotao Duan, Xiao Pan, Cheng Chen, Shiqi She, Tong Su, Jingchao Li, Xinyu Chen, Yanwen Zhou, Wenjie Chen, Xiaobing Zhang, Xiaoyun Pan, Ying-Jie Wang, Bo Kang
Summary: LSD1-mediated demethylation of OCT4 plays a crucial role in restricting the 'locked-in' mode binding of OCT4 PORE-homodimers to the PORE-motif-containing genes, thereby maintaining pluripotency.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Wenjie Chen, Xinyu Chen, Xiaobing Zhang, Cheng Chen, Songsong Dan, Jianwen Hu, Bo Kang, Ying-Jie Wang
Summary: The study revealed the crucial role of SOX2 interaction with DNA repair proteins in regulating neural progenitor cells, and significant up-regulation of biomarkers transcriptionally suppressed by the SOX2/DNA repair protein complexes can be achieved by down-regulating two DNA repair proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Cheng Chen, Xiaobing Zhang, Yisha Wang, Xinyu Chen, Wenjie Chen, Songsong Dan, Shiqi She, Weiwei Hu, Jie Dai, Jianwen Hu, Qingyi Cao, Qianyu Liu, Yinghua Huang, Baoming Qin, Bo Kang, Ying-Jie Wang
Summary: RNA processing and translation are identified as the most differentially regulated processes between naive and primed human embryonic stem cells (hESCs) through quantitative proteomics, phosphoproteomics, and acetylproteomics analyses. Naive hESCs have a more compact proteome and can translate selective mRNAs through both eIF4E-dependent and eIF4E-independent/eIF4A2-dependent pathways. These findings contribute to a better understanding of the potential of naive hESCs and the optimization of conditions for clinical applications.
Article
Oncology
Bo Kang, Xiaobing Zhang, Weibing Wang, Shiqi She, Wenjie Chen, Cheng Chen, Yisha Wang, Xiaoyun Pan, Ouyuan Xu, Yingjie Wang
Summary: This study found that the combination therapy of the novel drug PB-020 with mebendazole (MBZ) or anti-PD-1 antibodies has a synergistic effect in colorectal cancer (CRC). It reduces cell viability and blocks tumor progression, as well as suppressing signaling pathway genes associated with drug resistance. These findings provide preclinical evidence for the combined treatment of CRC with PB-020 and clinically approved anticancer drugs.
Article
Plant Sciences
Shi-Qi She, Yang Zhang, Jia Yang, Chun-Mei Pang, Yue-Liang Xu, Zheng-Hai Chen
Summary: In this study, a new species called Allium tianmuense is described based on morphological and molecular analyses, and its taxonomic relationships are discussed. It is found in the understorey of deciduous broad-leaved forests at an elevation of 1000-1500 m in Tianmu Mountain, Zhejiang, China. Morphological analysis reveals that A. tianmuense belongs to A. sect. Sacculiferum and differs from common species like A. thunbergii and A. chinense in terms of leaves, umbel, filaments, and phenology. Phylogenetic analysis of nuclear ribosomal DNA internal transcribed spacer (ITS) confirms the inclusion of A. tianmuense in A. sect. Sacculiferum and identifies it as a distinct species within this section.
