4.7 Article

Bioactive peptides in beef: Endogenous generation through postmortem aging

Journal

MEAT SCIENCE
Volume 123, Issue -, Pages 134-142

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2016.09.015

Keywords

Bioactive peptides; Postmortem aging; Tenderness; Beef; Collagen

Funding

  1. Future Food Innovation
  2. regional consortium of Central Denmark
  3. Graduate School of Science and Technology at Aarhus University

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The present research was performed to investigate endogenous release of bioactive peptides in beef during postmortem aging times (1, 10 and 20 days). Gradually decreased Warner-Bratzler shear force (WBSF) values of longissimus thoracic (LT) and semitendinosus (ST) muscles were observed and the degradation of structural proteins and collagen led to release of low-molecular weight (<3 kDa) peptides. These peptides exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, ACE- and renin-inhibitory activities. The peptide sequences were identified by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). In silico analysis (PeptideRanker and BIOPEP) of their bioactivity potentials demonstrated peptides with the predicted bioactivity scores (>0.8) as well as collagen peptides with bioactivity scores (0.6-0.8). The present findings provide insights on development of healthy beef through postmortem aging at 4 degrees C (C) 2016 Elsevier Ltd. All rights reserved.

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